• 한국양식학회지
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• 제19권4호
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• pp.299-304
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• 2006

넙치 치어용 건조사료의 적정 단백질 및 지질 함량을 조사하기 위하여 사료의 단백질 함량을 50% 및 55%로 조절하고, 각 단백질 함량에 지질 함량을 8%, 12% 및 15%로 조절하여 6종류의 실험 EP 사료를 제조하였다. 21 g의 넙치 치어를 300 L 수조에 3반복으로 무작위로 수용하여, 평균 수온 $20\;^{\circ}C$에서 1일 2회 만복으로 EP 사료를 9주간 공급하였다. 사육실험 결과, 생존율, 증중률 및 일일사료섭취율은 사료의 단백질 및 지질 함량에 영향을 받지 않았다(P>0.05). 사료효율과 단백질효율은 사료의 지질 함량에 유의하게 영향을 받았으며(P<0.001), 사료 지질 함량이 증가함에 따라 증가하는 경향을 보였다. 사료효율은 단백질 50%에 지질 15% 공급구와 단백질 55%에 지질 15% 공급구 사이에 유의차는 없었지만, 다른 실험사료 공급구보다 높았다(P<0.05). 단백질효율도 사료효율과 유사한 경향을 보였고, 단백질 55%에 지질 15% 공급한 실험구가 단백질 50%에 지질 8%, 단백질 55%에 지질 8% 및 지질 12% 공급구보다 높았다(P<0.05). 어체 간의 수분, 단백질 및 지질 함량은 사료의 지질 함량에 유의하게 영향을 받았다(P<0.01). 근육의 일반성분은 실험구간에 유의차가 없었다(P>0.05). 이상의 결과로부터, 본 실험에 적용된 사육조건에서 넙치 치어용 EP 사료의 단백질 함량이 $50{\sim}55%$일 때, 지질 함량을 높여주는 것이 사료이용 효율을 개선시키는데 도움이 될 것으로 판단된다.

• Journal of Nutrition and Health
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• 제34권8호
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• pp.843-849
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• 2001

This study was performed to investigate the effect of n-3 fatty acids and dietary protein levels on renal function. Fifteen-month old male Sprague-Dawley rats were divided into 4 diet groups. Two-month old rats were used as a control group. The experimental diets contained either a% or 25% casein and lipid levels of the diets were 20% by weight. For the control group, the lipid was composed of beef tallow and corn oil on a 1:1 basis, and fish oil was comprised 75% of the fat mixture for the fish oil group. Rats were fed the diets ad libitum for 8 weeks. GFR and urinary protein excretion were higher in high protein groups, while fish oil exhibited no effects. Renal medulla TXB$_2$and PGE$_2$ concentrations tended to be higher in high protein groups and lower in fish oil groups. Light microscopic examinations showed that glomerulosclerosis, tubular atrophy, tubular cast, interstitial inflammation and interstitial fibrosis fended to be higher in aged rats and in high protein groups and lower in fish oil groups. Serum levels of total lipid, triglyceride and total cholesterol were higher in aged rats and lower in fish oil groups while serum HDL-cholesterol level was higher in young rats and in fish oil groups. However, dietary protein level had no effect on serum lipid levels. Serum TBARS concentration was higher in aged rats and in fish oil groups. In conclusion, fish oil caused changes in serum lipid concentrations and eicosanoids metabolism. The effect of fish oil on renal function was less obvious than dietary protein. However, fish oil seemed to be effective in lessening deterioration of renal function due to aging and/or high protein diets through changes in lipid and eicosanoids metabolism.

• 대한가정학회지
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• 제18권4호
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• pp.103-107
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• 1980

This study was conducted to compare the effect of dietary sucrose and starch on the lipid metabolism of Rat fed by low protein diet for 3 and 6 weeks periods. Forty male weanling Spargue-Dawley rats weighing 52.7 grams each, after being adopted for 2 days with standard diet, were blocked into 8 groups and fed experimental diet as designed. Experimental diet were composed of two different levels of proteins, 6% designed as low protein diet and 15% designed as standard protein diet. In each group, the content of serum lipid, serum cholesterol and liver lipid were measured. The results of this experiment were summerized as follows. 1. liver lipid content was tended to be high in the sucrose group of low protein fed animal. 2. the content of total serum lipid was tended to be high in the sucrose group, and this tendency was showed statistical significance in the animals fed by low and standard protein after 6 weeks of experimental period. 3. The difference in the total serum cholesterol content between the sucrose and starch group was not significant

• Journal of Nutrition and Health
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• 제19권3호
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• pp.177-189
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• 1986

This study was undertalien to investigate the effects of dietary protein sources on protein, lipid, Ca. metabolism and immune response in college women (20), 16 of whom had Jupito constitutions and 4 had Hespero constitutions based on Kwon's theory . They were divided into 3 groups according to the main dietary protein source ; Beef group, Yellow tailrunner group and Soybean durd group. The menu of experimental diet of 3 group were same except their main dietary protein source(beef, yellow tailrunner, soybean curd). They were fed experimental diet for 4 days and their food intake was not restricted. Beef group in Jupito constitutions and yellow tailrunner group in Hespero constitutions were expected to present better effects than the other groups, because beef and yellow tailrunner are good for Jupito constitutions and Hespero constitutions, respectively. Results of Beef group in Jupito constitutions and Hespero constitutions, respectively. Results of Beef group in Jupito constitutions and yellow tailrunner group in Hespero constitutions were as following. 1) Calcium retention rate, Calcium apparent digestibility, serum Complement 3 concentration and LDL+ VLDLconcentration were higher in both groups. 2) Serum HDL concentration and immunoglobulin G concentration were lower in both groups than in the other groups. According to the main dietary protein sources, noted results were as following. 1) Serum total lipid and total cholesterol concentration were the significantly lowest in Soybean curd group. 2) Nitrogen retention rate was the significantly highest in Beef group.

• Fisheries and Aquatic Sciences
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• 제12권2호
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• pp.104-110
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• 2009

Feeding trials were performed at two different water temperatures (summer and winter seasons) to identify suitable protein and lipid (energy) levels to be used in formulating extruded pellets for olive flounder. Experiments were conducted to determine the effects of protein and lipid levels in extruded pellets on the growth and body composition of the flounder in both feeding trials. Six experimental diets were prepared containing three protein levels (46%, 51%, and 56%) and two lipid levels (10% and 17%). In the first experiment, during the summer season ($22{\pm}2.2^{\circ}C$), a triplicate group of fish (initial weight, 114 g) were fed to satiation one of the six diets for 11 weeks. The highest weight gain was observed in fish fed the 56/17 (% protein/% lipid) diet, but this weight gain was not significantly different from that of fish fed the other diets, except for those fed the 46/10 diet. The feed efficiency and protein efficiency ratio of fish fed the 17% lipid diets were higher than those of fish fed the 10% lipid diets at each protein level. In the second experiment, during the winter season ($13{\pm}1.5^{\circ}C$), a triplicate group of fish (initial weight, 107 g) were fed to satiation one of the six diets for 9 weeks. Weight gain was not significantly different among all groups. The feed efficiency and protein efficiency ratio tended to increase with increasing dietary lipid level at each protein level. The whole-body crude lipid content of the of fish fed the 17% lipid diets was significantly higher than that of fish fed the 10% lipid diets at each protein level in both feeding trials. Based on the data obtained in this study, the inclusion of dietary protein at a level of 46% appears to be sufficient to support optimal growth, and increasing the dietary lipid level from 10% to 17% had no beneficial effects on the growth and feed utilization of olive flounder (110-300 g), except for fish fed a 56% protein diet in the summer season.

• 한국양식학회:학술대회논문집
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• 한국양식학회 2003년도 추계학술발표대회 논문요약집
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• pp.17-18
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• 2003

Although flounder is one of the most important marine fish for aquaculture in Korea, feeding the flounder in commercial farms depends mainly on moist pellet in which over 70% frozen fishes (e.g. frozen horse mackerel) are incorporated in its formulation. Therefore, for further expansion of flounder farming, it is essential to employ practical formulated feeds that can support reasonable growth. Development of nutritionally balanced and cost-effective feeds is dependant on the information about nutritional requirement and feed utilization of the species. Nutrient and energy source in feed are needed for the growth and maintenance of fish. Protein is probably the most important nutrient affecting fish growth and feed cost. Therefore, it is essential to determine the optimum dietary protein level for the growth of fish, both its high proportion in the feed and because it is the main factor in determining feed cost. Dietary energy level is also critical because protein source in the feed is utilized as an energy source when the feed deficient in energy is fed to fish, whereas when the feed excess in energy is fed to fish, feed consumption decreased and resulted in growth reduction due to lack of other necessary nutrients for normal growth. Improper dietary protein, energy levels and/or their ratio will lead to an increase of fish production cost and deterioration of water quality resulting from wasted feed; thus, they are important in formulating commercial feed. Dietary lipids play important roles in providing energy and essential fatty acid for normal growth and survival of fish. Although carbohydrates are not essential nutrients for carnivorous fish, these compounds play important roles as a low-cost energy source for protein sparing and also as a feed binder. Nutrition researches for flounder have identified its requirements of protein, lipid and essential fatty acid, vitamin, and minerals for normal growth. Other studies have also been carried out to investigate the utilization of the protein, lipid and carbohydrate sources. Based on these nutritional information obtained, practical feed formulations have been studied for improve aquaculture production of flounder. The results of the researches on utilization of dietary protein, lipid and carbohydrate by flounder are discussed in this review.

• 대한영양사협회학술지
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• 제14권4호
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• pp.371-381
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• 2008

In order to elucidate the effects of a lacto-ovo vegetarian diet on serum lipid levels, 91 male collegians residing in the Seoul area were recruited. The vegetarians did not smoke tobacco nor drink alcohol. Their anthropometric measurements, dietary intakes, and blood lipid concentrations were compared to age-matched omnivorous controls. The subjects were divided into four groups. The four groups were: 1) NSNDV (n=25): Non Smoking Non Drinking Vegetarians, 2) NSNDO (n=10): Non Smoking Non Drinking Omnivores, 3) SDO (n=37): Smoking, Drinking Omnivores, 4) SDM (n=11): Smoking Drinking Meat-eaters. Total serum cholesterol, HDL-cholesterol and hematocrit were analyzed. The results were as follows: Weight (p<0.05) and BMI (p<0.01) were significantly higher in SDM than NSNDV. Dietary protein (p<0.01), iron (p<0.05) and dietary lipids (p<0.01) from animals in NSNDV were significantly lower than the results in the SDM. Interestingly enough, we noted no significant differences between the four groups in terms of average total dietary protein, dietary lipids and dietary iron, total serum cholesterol, or HDL-cholesterol. Dietary fiber intake was significantly higher in NSNDV than in SDM (p<0.05). Weight, BMI, and total cholesterol were significantly positively correlated with animal protein (p<0.05) and animal lipid (p<0.01) intake. These results showed that vegetarian collegians who haven't smoked tobacco nor drunk alcohol evidenced lower body weight, BMI, and dietary animal lipid intake, but higher dietary fiber intake, than the other three groups. Additionally, intake of animal protein (p<0.001) and intake of animal lipids (p<0.001) were significantly positively correlated with body weight, BMI, and total serum cholesterol in male collegians.

• Journal of Nutrition and Health
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• 제23권7호
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• pp.492-503
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• 1990

This study was performed to investigate nutritional effect of various dietary fibers on lead absorption, and protein and lipid metabolisms in growing rats. Sixty male rats of Sprague-Dawley strain weighing 140$\pm$1.1g were blocked into 10 groups according to body weight and fed 10 kinds of diet different with fiber sources [non-fiber, cellulose, pectin, guar gum or carboxymethylcellulose(CMC)] and lead levels (0 or 1%) for 4 weeks. Results were summerized as follows : 1) Food intake, weight gain, FER and PER were remarkably decreased in lead(Pb)-added groups. Weight gain, FER and PER in Pb-added pectin group were significantly lower than those in Pb-added non-fiber group. 2) Liver and kidney weights, femur weight and length, hematocrit and hemoglobin content were decreased in Pb-added groups. Especially femur and liver weights in pectin groups were the lowest among groups. 3) Total protein content in serum was significantly decreased in Pb-added groups but was not different with dietary fiber sources. Total lipid content in serum was not different with dietary Pb levels and fiber sources, but cholesterol content in serum of guar gum group was significantly decreased by Pb addition. 4) Nitrogen, lipid and cholesteol contents in liver were significantly decreased in Pb-added groups, and lipid content in liver of pectin and CMC groups was lower than other groups. 5) Daily urinary and fecal excretions of nitrogen, kipid and cholesterol were decreased in Pb-added groups, and fecal nitrogen was significantly increased in Pb-added groups, and fecal nitrogen of cellulose and guar gum groups was significantly higher than other groups. Fecal excretions of lipid and cholesterol were increased by dietary fibers, and especially fecal lipid was remarkably increased in pectin and guar hum group. 6) Pb contents in liver and femur were decreased by dietary fibers. Especially Pb contents in liver, kidney and femur were significantly decreased in guar gum group. 7) Daily urinary and fecal excretions of Pb were significantly increased in cellulose and guar gum groups, and fecla excretion of Pb in guar gum group was twice of non-fiber group. Pb absorption ratio was significantly decreased in guar gum group. In conclusion, dietary fibers have effect on protein and lipid metabolisms, and decreased intestinal absorption of Pb by increasing fecal excretion. But the degree of effect was different with dietary fiber sources.

• 동아시아식생활학회지
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• 제8권2호
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• pp.107-115
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• 1998

This study was conducted to investigate the effects of dietary fibers (cellulose ; polydextrose ; pectin ; ricebran) supplementation on the kidney fucntion of hyperlipidemic-induced rats on a high cholesterol diet (5% cholesterol). Serum lipid concentrations were not influenced by dietary fiber sources, but the pectin group was effective in reducing serum lipid levels. Total serum protein and albumin concentrations tended to higher in the polydextrose group, and serum creatinine and urea-N concentrations were higher in the pectin group than those in the other groups. Even though total urinary protein was high in the pectin group, GFR was reduced (18~30% decrease) as compared to other groups. The urea-N level was elevated in the polydextrose group as compared to that of cellulose group(25% increase). Total lipid, triglyceride and cholesterol concentrations in the liver of pectin group were lower than those of other groups. Total lipid and cholesterol concentrations in the kidneys were reduced in pectin and ricebran groups, respectively. The total cholesterol concentration infecal was significantly high in the polydextrose and pectin groups(p<0.05), and the triglyceride was highest in the pectin group. These results indicate no significant effects of dietary fiber supplementation on the kidney function of hyperlipidemic-induced rats on a high cholesterol diet, but pectin was very effective to improve lipid metabolism and to reduce GFR.

• 한국수산과학회지
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• 제42권4호
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• pp.344-348
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• 2009

This study was conducted to investigate effects of dietary lipid level on growth and body composition of juvenile red sea bream in low temperatures. Duplicate groups of fish (initial body weight of 79 g) were fed one of three isonitronic diets (47% crude protein) containing different lipid levels (10%, 17% and 22%) for 25 weeks during the winter season. Weight gain and survival of fish fed 17% lipid diet were significantly (P<0.05) higher than those fish fed the 10% or 22% lipid diet. Protein efficiency ratio, daily feed intake, condition factor, hepatosomatic index and viscerasomatic index were not affected by dietary lipid level, but feed efficiency of fish fed 10% lipid diet was significantly (P<0.05) lower than those fish fed the 17% or 22% lipid diet. Proximate composition of the whole body, liver, viscera and dorsal muscle were not significantly different among all groups except for crude protein content of dorsal muscle. The contents of 16:0, 18:0, 20:4n-3 and 20:5n-3 of the whole body were significantly (P<0.05) affected by dietary lipid level. The results of this study suggest that an increased dietary lipid level from 10% to 17% can improve growth of juvenile red sea bream in low temperature periods.