• Journal of Nutrition and Health
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• v.33 no.7
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• pp.712-716
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• 2000

The Purpose of this study is to investigate the effect of soluble dietary fiber psyllium on insulin sensitivity and skeletal muscle glucose transporter 4(GLUT4) protein expression in stroke-prone hypertensive rats(SHRSP) fed a high-fat diet containing 5% of psyllium or cellulose from five to nine weeks of age. Obtained results were as follows : (1) In the psyllium diet group fasting plasma glucose level was significantly reduced and glucose levels upon oral glucose tolerance test were significantly lower than cellulose diet group at 30 min(p<0.05) and 60 min(p<0.01) (2) Skeletal muscle GLUT4 contents were significantly increased in the soleus(slow twitch) and extensor digitorum longus(fast twitch) muscle of psyllium diet group. (3) However there was no difference in insulin levels in the fasting and oral glucose tolerance test. These results indicated that psyllium diet improves peripheral insulin sensitivity but not insulin secretion. In conclusion our present finding suggest that soluble fiber diet is effective to increase insulin sensitivity in SHRSP. From these results it was suggested that soluble dietary fiber supplementation effectively prevents insulin resistance.

• Journal of Nutrition and Health
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• v.30 no.8
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• pp.895-903
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• 1997

This study was performed to investigate the influence of dietray food from the three CHinese medicinal herbs(Mugwort, Dried organge peel and Duchung) on lipi d metabolism in rats. Male rats of Sprague-Dawley strain were divided into four dietary groups(Cellulose (CL), Mugwort(MW), Dried organge peel9OP), Duchung(DC)) after three weeks of hyperlipidemic dieting and were fed ad libitum each of the experimental diets for six weeks. The fiber sources of the experimental diets were prepared by drying and milling mugwort, orange peel, duchung and cellulose. Each of the Fiber sources was mixed into the diet to achieve 10g% levels of total dietary fiber. Levels of apparent fat absorption exhibited a significant decrease in the MW and DC groups. The OP group also showed a decreasing trend, but to a lesser extent. The levels of serum total lipid were not significantly different among the four experimental groups. Total serum cholesterol levels were significantly lower in the OP grop that in both the CM and MW group. Serum TG and LDL-cholesterol levels were significantly higher in the DL group than other groups. Serum HDL-cholesterol levels were significantly levels were significantly lower in the CL group than in the other groups. Total lipid and total cholesterol in liver were significantly higher in the CL group than in the other groups. Total lipid and total cholesterol in feces displayed a significantly increase in the MW and DC groups when compared with the CL group. The OP group also showed an increase compared to the CL group.

• Korean Journal of Poultry Science
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• v.12 no.2
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• pp.97-105
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• 1985

Fifteen 1-yr old roosters were employed in order to investigate the influence of various dietary fibers on the water and Na metabolism of chicken. Wheat bran, pure cellulose and ground rice straw replaced corn in the control diet at 10% level; Pectin replaced corn at 8% level. Three roosters were allotted per dietary treatment and one rooster per replicate. The limit of probability accepted as being significant was P$\leq$.05. The dry matter metabolizabilities of cellulose and rice straw diets were significantly lower, and those of control and pectin diets were significantly higher than that of the wheat bran diet. Pectin fed roosters seemed to drink more water than did the other birds. Also, excreta from roosters fed the pectin diet were significantly wetter than those from other treatments. However, no difference in water intake and fecal moisture were found when !he roosters were colostomized. It was also found that the colostomized birds drank more water than did intact ones. Data of water holding capacity and bulk density of dried excreta showed that cellulose, rice straw and wheat bran in decreasing order were strong in fibrous property. There were no significant differences in C$_{Na+}$ , Cosm and C$_{{H_2}O}$ among all treatments. When the values of urine volume, urine osmolality and C$_{{H_2}O}$ were put together, it can be concluded that 11 out of 15 roosters developed diuresis. More severe diuretic symptoms were developed in roosters when they were fed more fibrous diets. In conclusion, when the four dietary fibers were graded in terms of fibrous property, it turned out to be pectin, cellulose, rice straw and wheat bran in decreasing order.

• Journal of Nutrition and Health
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• v.38 no.10
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• pp.817-826
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• 2005

Recently it has been reported that vitamin A and retinol binding proteins (RBPs) in blood and urine were changed in the condition of diabetes mellitus or hyperlipidemia. Fruits and vegetables are recommended to consume for the people suffered from these chronic degenerative diseases. The main components of fruits and vegetables are dietary fibers, for example cellulose and pectin, of which function to affect the absorption and excretion of dietary fat and fat-soluble substances. This study was conducted to investigate the effect of dietary fibers on RBPs mRNA expression in liver, small intestine and serum of rat fed high fat diet during 4 weeks. Sprague-Dawley rats, weighing 121g on average, were divided into four groups; (Control; $17\%$ fat & cellulose supplement diet, HF0: $25\%$ fat & fiber free diet, B:.Uc: $25\%$ fat & cellulose supplement diet and HF0: $25\%$ fat & pectin supplement diet) . The rats fed high fat diet groups (HF0, HFC, HFP) tended to consume the food less than the control group, but FER of HF0 groups was significantly higher than the control (p < 0.05) . The weight of adrenal gland in high fat diet groups (HF0, HFC, HFP) was significantly less than the control. Total lipid in feces daily excreted and in liver did not show any significant differences among the groups. Total cholesterol in HFP group was significantly different from that of HFC group. Serum total cholesterol and triglyceride in other group tended to lower than other groups and HDL cholesterol higher. Consequently, AI (atherogenic index) was the lowest in HFP group. Vit A contents in feces daily excreted tended to lower in high fat diet groups (HF0, HFP) compared to the control group. That content in adrenal gland was the lowest in HF0 group, but not in liver. In HFP group were down-regulated cRBPI mRNA in liver and cRBPII mRNA in small intestine and up-regulated RBP and transthyretin expression in serum compared to the other groups. In conclusion, dietary fibers, especially pectin, in high fat diet might down-regulate the expression of CRBP I, CRBP II mRNA in liver and small intestine, but increase the secretion of RBP into serum and therefore inhance the bioavailability of Vit A through the body. (Korean J Nutrition 38(10): 817$\sim$826,2005)

• Journal of the Korean Society of Food Science and Nutrition
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• v.23 no.4
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• pp.541-541
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• 1994

이유기를 지난 웅성흰쥐를 대조식이 (C), 고지방식이 (HF) 및 고지방 고에너지식이 (HFHE) 로 4 주간 사양하고 일부를 희생시킨 후 , HF 군과 HFHE 군 일부에식이섬유 pectin 과 cellulose를 각각 첨가한 HF-P , HF-C, HFHE-P , HFHE-C 식이를 C, HF ,HFHE 식이와 함께 4 주간 더 사양한 후 희생시켜 체중증가량, 총 에너지섭취량, 총식이섭 취량, 식이효율, 간장과 비장의 무게, 혈중 지질의 성상 및 간세포에 미치는 영향을 비교 관 찰한 결과 체중증가는 C 군, HF 군, HFHE 군간의 유의적 차이는 없었으며, 식이섬유 첨가 시 모두 체중이 감소되었다. 총에너지 섭취량은 C 군이 가장 많았고, 식이효율은 HF-HE군 이 가장 높았으며, 식이섬유 첨가에 의해서는 영향을 받지 않았다. 간장의 무게는 C군 , HF 군, HFHE 군 모두 식이의 영향이 없었고, pectin 과 celulose 첨가에 의한 변화도 나타내지 않았다. 비장의 무게는 C군 보다 HF 군과 HFHE 군이 감소하였으나, pectin 과 cellulose 첨가에 의해서는 변화가 없었다. 혈중 총 지질, 총콜레스테롤, 중성지방 및 인지질의 함량은 각 실험군모두 실험기간이 경과함에 따라 증가되었으며, 특히 HFHE 군의 유의성있게 증가 하였고 pectin 과 cellulose를 첨가하였을때는 감소하였으며 pectin이 cellulose 보다 감소효 과가있다.

• Journal of the Korean Society of Food Science and Nutrition
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• v.20 no.2
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• pp.167-172
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• 1991

The contents of dietary fiber and its physical properties of vegetables were determined. The samples selected in this study were soybean sprouts (Glycine max MERILL sprouts) butterber (Petasites japonicus MIQ), taro stem(Colcasia antiquorum SCHOTT stem), bracken (Pteridium aquilinum KUHN)and dried Korean cabbage leaves(Brassica compestris L.leaves). The contents of dietary fiber were 20.96~50.62% of neutral detergent figer, 10.60-38.19% of acid detergent fiber, 1.74-10.88% of lignin, 5.79~33.29% of hemicellulose and 5.79-33.29% of cellulose. Butterber had the highest dietary fiber content. Mean waterholding capacity of samples was 5.65-12.26g.water/g.acetone dried powder and butterber and dried Korean cabbage leaves had higher water holding capacity value than the others. Densities were 140-250mg/ml of direct density and 385-417 mg/ml of bulk density.

• Journal of the East Asian Society of Dietary Life
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• v.10 no.5
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• pp.439-444
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• 2000

호알칼리성 방선균 Streptomyes sp. B-2를 Glucose Isomerse 생성을 위해 토양에서 분리했다. Glucose Isomerase(G.I)는 high fructose glucose syrup과 fructose의 생산을 위해서 식품 공업에서 아주 중요시되고 있는 효소이다. 호알칼리성 방선균 Streptomyces sp. B-2가 생성하는 glucose isomerase(G.I.)를 정제하였다. G.I.는 (NH$_4$)$_2$So$_4$분획, DEAE-cellulose, Sephadex G-200 chromatography하여 순수 분리 하였다. 순수분리된 G.I.는 electrophoresis에 의해 확인을 했다. SDS-acrylamide gel electrophoresis에 의해 정제된 효소는 single band를 보여주었다.

• Journal of the Korean Home Economics Association
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• v.29 no.3
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• pp.47-59
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• 1991

This study wa performed to investigate the effects of dietary protein and fiber on the lead and protein metabolism in lead poisoning rats. Seventy male rats of Sprague-Dawley strain weighing 172$\pm$2g were blocked into 14 gropus according to body weight. Protein(casein) was given at levels of 15 or 40%, and fibers(pectin, cellulose and CMC) were given at levels of 0, 4 or 10%. The results are summarized as follows: 1. Food intake, weight gain and food efficiency ratio(FER) in groups fed high protein diets were higher than those in low protein groups. Liver weight in groups fed no dietary fiber was higher than that of animals fed fiber. Kidney and femur weights were greater in high protein groups. Tibia and femur lengths, and tibia weight were not significantly different among groups. 2. Hemoglobin content and hematocrit values showed no significant differance with dietary factors. 3. Total protein contents of serum and liver showed no significant difference, but tended to increase with increasing dietary protein level. Both daily urinary and fecal nitrogen excretions in high protein groups were higher than those in low protein groups. Especially daily fecal nitrogen excretions in high dietary fiber groups were significantly high. Body nitrogen absorption rate was the highest in animals fed no fiber. 4. Pb levels in blood, liver, kidney and bone tended to decrease with high dietary protein and fiber levels. Especially Pb level of kidney was high in all groups. Daily urinary Pb excretion showed no significant difference with dietary factors, but fecal Pb excretion increased significantly in high protein and fiber groups.

• Korean Journal of Food Science and Technology
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• v.19 no.4
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• pp.317-323
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• 1987

This study was undertaken to examine the applicability of domestic enzymes in the quantitative determination of dietary fibers according to the official enzymatic-gravimetric method of AOAC and to apply it to 4 kinds of fruits (apple, pear, peach and persimmon) and 4 kinds of vegetables (Korean radish, lettuce, Korean cabbage and cabbage Kimchi). With domestic enzymes, an optimum condition was selected to use 1/10 units of enzyme activity and to extent the reaction time two-fold as compared with the recommended method, in the case of fruits and vegetables. On a dry matter basis, fiber contents of fruits were in the range of 9.4-28.8% total dietary fiber, 1.8-7.8% non-cellulosic polysaccharides, 3.7-5.8% cellulose and 1.3-21.3% lignin. Fiber contents of vegetables were 26.0-35.7% total dietary fiber, 11.3-14.4% non-cellulosic polysac-charides, 12.3-19.7% cellulose and 1.4-7.4% lignin. On a dry matter basis, crude fiber contents were 3.5-6.7%in fruits and 9.1-13.8% in vegetables. Therefore, crude fiber contents of fruits and vegetables accounted for only 12-50% of total dietary fibers.

• Korean Journal of Food Science and Technology
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• v.33 no.2
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• pp.216-220
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• 2001

This study was conducted to examine the effect of oxidized cellulose that had been selectively oxidized with TEMPO(2, 2, 6, 6-tetramethyl-1-piperidine oxoammonium ion), NaBr and NaOCl substituting for 10% of the wheat flour in a yellow layer cake formula. Physicochemical and sensory characteristics of a cake containing oxidized cellulose were compared with a control cake and a cake containing non-oxidized cellulose(CNC). The cake sample with oxidized cellulose(COC) had smaller L and a values and greater b value than the control, with a greater cake volume. Results of sensory evaluation showed that grittiness of COC was about the same as that of the control, but weaker than that of CNC, and COC was more moist and less hard than the control. These observations indicated the possibility that the oxidized cellulose could be used as a dietary fiber in a cake without causing detrimental effect on textural characteristics.