Effect of Dietary Fibers on Retinol Binding Protein (RBP, cRBP I, cRBP II) Gene Expression in Rats Fed High Fat Diet

고지방 식이내 식이섬유질이 흰쥐의 레티놀 결합 단백질 (RBP, cRBP I, cRBP II) 유전자 발현에 미치는 영향

  • Jo, Min-Wha (Department of Food and Nutritional Science, Kyungnam University) ;
  • Shin, Dong-Soon (Department of Food and Nutritional Science, Kyungnam University)
  • 조민화 (경남대학교 식품영양학과) ;
  • 신동순 (경남대학교 식품영양학과)
  • Published : 2005.12.01

Abstract

Recently it has been reported that vitamin A and retinol binding proteins (RBPs) in blood and urine were changed in the condition of diabetes mellitus or hyperlipidemia. Fruits and vegetables are recommended to consume for the people suffered from these chronic degenerative diseases. The main components of fruits and vegetables are dietary fibers, for example cellulose and pectin, of which function to affect the absorption and excretion of dietary fat and fat-soluble substances. This study was conducted to investigate the effect of dietary fibers on RBPs mRNA expression in liver, small intestine and serum of rat fed high fat diet during 4 weeks. Sprague-Dawley rats, weighing 121g on average, were divided into four groups; (Control; $17\%$ fat & cellulose supplement diet, HF0: $25\%$ fat & fiber free diet, B:.Uc: $25\%$ fat & cellulose supplement diet and HF0: $25\%$ fat & pectin supplement diet) . The rats fed high fat diet groups (HF0, HFC, HFP) tended to consume the food less than the control group, but FER of HF0 groups was significantly higher than the control (p < 0.05) . The weight of adrenal gland in high fat diet groups (HF0, HFC, HFP) was significantly less than the control. Total lipid in feces daily excreted and in liver did not show any significant differences among the groups. Total cholesterol in HFP group was significantly different from that of HFC group. Serum total cholesterol and triglyceride in other group tended to lower than other groups and HDL cholesterol higher. Consequently, AI (atherogenic index) was the lowest in HFP group. Vit A contents in feces daily excreted tended to lower in high fat diet groups (HF0, HFP) compared to the control group. That content in adrenal gland was the lowest in HF0 group, but not in liver. In HFP group were down-regulated cRBPI mRNA in liver and cRBPII mRNA in small intestine and up-regulated RBP and transthyretin expression in serum compared to the other groups. In conclusion, dietary fibers, especially pectin, in high fat diet might down-regulate the expression of CRBP I, CRBP II mRNA in liver and small intestine, but increase the secretion of RBP into serum and therefore inhance the bioavailability of Vit A through the body. (Korean J Nutrition 38(10): 817$\sim$826,2005)

Keywords

References

  1. National health and nutrition survey. Ministry of Health and Welfare, 2001
  2. Statistics of cause death. Korea National Statistical Office, 2003
  3. Hwang SH, Kim JI, Sung CJ. Assessment of dietary fiber intake in Korean college students. J Korean Soc Food Nutr 25(2): 205-213, 1996
  4. Lim ML. Food and nutrient intakes, body consciousness and self-esteem in obese adolescent. Thesis for master degree, Kyungnam University, 2003
  5. Recommended dietary allowances for Korean. 7th revision. The Korean Nutrition Society, Seoul, 2001
  6. Kim MJ, Lee SS. The effect of dietary fiber on the serum lipid level and bowel function in rats. Korean J Nutr 28(1): 23-32, 1995
  7. Oh HI, Ly SY. A study on nutritional characteristics of common korean dietary fiber rich foods. J Korean Soc Food Nutr 27(2):296-304, 1998
  8. Yang JL, Sub MJ, Song YS. Postprandial plasma lipid levels and digestive enzyme activities after high fat meal in rats adapted to dietary fiber. J Korean Soc Food Nutr 26(1):116-122, 1997
  9. Bownman BA, Russel RM. Present knowledge in nutrition, eighth edition, International life Science, 2001
  10. World Health Organization. The global prevalence of vitamin A deficiency. Micronutrient series document, WHO/NUT/95.3, Geneva, 1995
  11. World Health Organization/UNICEF. Indicators for assessing vitamin A deficiency and their application in monitoring and evaluating intervention programmes. Micronutrient series document, WHOINUT/96.1O. Geneva, 1996
  12. Underwood BA. Vitamin A in animal and human nutrition, In: Sporn NB, Roberts AB, Goodman DS, eds. The Retinoids, Vol. 11, Academic Press, Orlando, 1999
  13. Furr HC, Clark RM. Intestinal absorption and tissue distribution of carotenoids. J Nutr Biochem 8(7): 364-377, 1997 https://doi.org/10.1016/S0955-2863(97)00060-0
  14. Basu TK, Basualdo C. Vitamin A homeostasis and diabetes mellitus. Nutrition 13(9): 804-806, 1997 https://doi.org/10.1016/S0899-9007(97)00192-5
  15. Lu J, Dixon WT, Tsin AT, Basu TK. The metabolic availability of Vitamin A is decreased at the onset of diabetes in BB rats. J Nutr 130: 1958-1962, 2000
  16. Vogel S, Mendelsohn CL, Mertz JR. Characterization of a new member of the fatty acid-binding protein family that binds alltrans-retinol. J Biol Chem 276(2):1353-1360, 2001 https://doi.org/10.1074/jbc.M005118200
  17. Xueping E, Zhang L, Lu J. Increased neonatal mortality in mice lacking cellular retinol-binding protein II. J Biol Chem 277(9): 36617-36623, 2002 https://doi.org/10.1074/jbc.M205519200
  18. Crow JA, Ong DE. Cell-specific immunohistochemical localization of a cellular retinol-binding protein (type two) in the small intestine of rat. Proc Natl Acad Sci USA 82(14): 4707-4711, 1985
  19. Ong DE. Retinoid metabolism during intestinal absorption. J Nutr 123(2):351-355, 1993
  20. Ong DE. Cellular transport and metabolism of vitamin A:roles of the cellular retinoid-binding proteins. Nutr Rev 52(2): 24-31, 1994
  21. Napoli JL. Biosynthesis and metabolism of retinoic acid. J Nutr 123(2): 362-366. 1993
  22. Ross A. Overview of retinoid metabolism. J Nutr 123(2): 346-350, 1993
  23. Bellovino D. Apreda M, Gragnoli S, Massimi M, Gaetani S. Vitamin A transport: in vitro models for the study of RBP secretion. Molecular Aspects of Medicine 24(6): 411-420, 2003 https://doi.org/10.1016/S0098-2997(03)00037-2
  24. Kazuhito S, Kazuki M. Transcriptional regulation of cellular retinol-binding protein, Type II gene expression in small intestine by dietary fat. Arch Biochem Biophysics 362(1):159-166, 1999 https://doi.org/10.1006/abbi.1998.1018
  25. Li E, Tso P. Vitamin A uptake from foods. Current Opinion of Lipidology 14(3):241-247, 2003 https://doi.org/10.1097/00041433-200306000-00003
  26. Folch J, Lees M, Sloanestanley GH. A simple method for the isolation and purification of total lipids from animal tissues. J Bio Chem 226(1): 89-91, 1957
  27. Handbook of experiments in food science and nutrition. The Korean Society of Food Science and Nutrition. Hyoil, Seoul, 2000
  28. Chang HK, Chung DH. Food analysis. Hyungseu, Seoul, 1999
  29. Torten J, Whitaker JR. Evaluation of the biuret and dye-binding determination in meats. J Food Sci 28: 168, 1964
  30. Kusunoki M, Hara T, Tsutsumi K, Nakamure T, Miyata T, Sakakibara F. The lipoprotein lipase activator, NO-1886, suppresses fat accumulation an insulin resistance in rats fed a high-fat diet. Diabetologia 43(7):875-880, 2000 https://doi.org/10.1007/s001250051464
  31. Chang YK, Youn HJ. The effect of dietary fat levels and sources of dietary fiber on serum and liver lipids of rats. Korean J Nutr 17(4): 253-261, 1984
  32. Fernandez ML, Sun DM, Tosca MA, Monarna DJ. Citrus pectin and cholesterol interact to regulate hepatic cholesterol homeostasis and lipoprotein metabolism. A dose-response study in guinea pigs. Am J Clin Nutr 59(4): 869-878, 1994
  33. Kirby RW, Anderson JW, Sieling B, Ress, ED, Chen I.W, Miller RE, Kay RM. Oat bran intake selectively lowers serum lowdensity lipoprotein cholesterol concentration of hypercholesterolmic men. Am J Clin Nutr 34(5): 824-829, 1981
  34. Vigne JL, Lairon D, Bore P. Effect of pectin wheat bran and cellulose on serum lipds and lipoproteins. Br J Nutr 58(3): 405-413, 1987 https://doi.org/10.1079/BJN19870109
  35. Kritchevsky D. Dietary fiber. Ann Rev Nutr 8:301-328, 1988 https://doi.org/10.1146/annurev.nu.08.070188.001505
  36. Ghyselinck NB, Bavik C, Sapin V. Cellular retinol-binding protein I is essential for vitamin A homeostasis. EMBO J 15(18):4903-4914, 1999
  37. Linder MC, Nutrition and metabolism of vitamin, In: Linder MC. ed. Nutritional Biochemistry and Metabolism, pp.70-131, Elsevier New York, 1985