Journal of the East Asian Society of Dietary Life (동아시아식생활학회지)

The East Asian Society of Dietary Life (동아시아식생활학회)
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Purification of Glucose Isomerase from ALkalophilic Streptomyces sp. B-2

호알칼리성 Streptomyces sp.B-2에 의한 Glucose Isomerase의 정제

  • 이은숙 (경산대학교 한의예과) ;
  • 이준우 (경북전문대학 식품가공과)
  • Published : 2000.10.01
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Abstract

호알칼리성 방선균 Streptomyes sp. B-2를 Glucose Isomerse 생성을 위해 토양에서 분리했다. Glucose Isomerase(G.I)는 high fructose glucose syrup과 fructose의 생산을 위해서 식품 공업에서 아주 중요시되고 있는 효소이다. 호알칼리성 방선균 Streptomyces sp. B-2가 생성하는 glucose isomerase(G.I.)를 정제하였다. G.I.는 (NH$_4$)$_2$So$_4$분획, DEAE-cellulose, Sephadex G-200 chromatography하여 순수 분리 하였다. 순수분리된 G.I.는 electrophoresis에 의해 확인을 했다. SDS-acrylamide gel electrophoresis에 의해 정제된 효소는 single band를 보여주었다.

Keywords

References

  1. Fermentation advances Streptomyces glucose isomerase Takasaki, Y.;Kosugi, Y.;D.perlman(ed.)
  2. Agr. Biol. Chem. v.29 Enzymatic conversion of D-glucose to D-fructose.Ⅵ. properties of the enzyme from Streptomyces phaeochromogenus Tsumura, N.;Sato, T.
  3. Science v.125 Enzymatic conversion of D-glucose to D-fructose Marshall, R. O.;Kooi, E. R.
  4. Appl. Biochem. BIoeng. v.2 Glucose isomerase production of high-fructose syrup Richard, L. A.;William, C.;Ben, J. S.
  5. Trans. R. Soc. London B. Biol. Sci. v.300 The production of industrial enzymes. philos Lambert, P. W.;J. L. Meers.
  6. Agr. Biol. Chem v.29 no.12 Enzymatic conversion of D-glucose to D-fructose. Ⅴ. partial purification and properties of the enzyme from Aerobacter cloacae Tsumura, N.;Sato, T.
  7. Agr. Biol. Chem v.30 no.12 Studies on Sugar-isomerizing enzyme. Production and utilization of glucose isomerase from Streptomyces sp. Takasaki, Y.
  8. Methods in Enzymology v.3 Colorimetric analysis of Sugar Ashwell, G.
  9. Biochim. Biophys. Acta. v.151 Purification, crystallization and properties of the D-xylose isomerase from Lactobacillus brevis Yamanaka, K.
  10. Clin. Chem. v.8 Gel electrophoresis Raymond, S.;Nakamichi, M.
  11. Process Biochem. v.June/July Glucose isomerase(a review) Chen, W-P.
  12. Process Biochem. v.August/September Glucose isomerase(a review) Chen, W-p.
  13. Enzyme. Microb. Technol. v.10 D-xylose isomerase from Streptomyces violaceoruber: Structural and Catalytic roles of bivalent metal ions Callens, M.;Kersters-Hilderson, H.;Vangrysperre, W.;De Bruyne, K.
  14. Biochem. J. v.273 Purification and characterization of thermostable glucose isomerase from Clostridium thermosulfurgenes and Thermoanaerobacter strain B6A Lee, Chanyoung;Zeikus, J. Gregory.
  15. Agric. Biol. Chem. v.37 Purification and properties of L-arabinose isomerase from Streptomyces sp. Yamanaka, K.;Isumori, K.