• Journal of Life Science
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• v.25 no.11
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• pp.1214-1222
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• 2015

The purpose of this study was to determine the effect of Pueraria lobate-root based combination supplementation containing Rehmannia glutinosa and exercise on histone modification in ovariectomized rat hindlimb skeletal muscle. Sixty rats were fed with high fat diet and randomly assigned into the following groups for 8 weeks: 1)HSV; High fat+Sedentary+Vehicle, 2)HSP; High fat+Sedentary+PR, 3)HSH; High fat+Sedentary+Estradiol, 4)HEV; High fat+Ex+Vehicle, 5)HEP; High fat+Ex+PR, 6)HEH; High fat+Ex+Estradiol. Exercise consisted of low intensity treadmill exercise(1-4th wk:15 m/min for 30 min, 5-8th wk: 18 m/min for 40 min, 5 times/week). The result of this study showed that exercise and Pueraria and Rehmannia glutinosa intake suppressed weight gain. Furthermore, exercise and Pueraria and Rehmannia glutinosa intake increased muscle mass. This study observed H3K9 acetylation and demethylation in plantaris muscle in exercised group, but no difference in soleus muscle. To test whether the decrease in HDAC4, HDAC5 and G9a mRNA levels after exercise and Pueraria/Rehmannia glutinosa intake, HDAC4, HDAC5 and G9a mRNA levels were determined by real-time PCR. Only exercise induced HDAC5 and G9a mRNA reduction in plantaris muscle, but not in soleus muscle. In conclusion, these data demonstrates that exercise and Pueraria/Rehmannia glutinosa intake effect on body compositions. These changes are regulated by epigenetic modifications, such as histone acetylation and methylation. Future studies should focus on gene-specific epigenetics and other epigenetic mechanism for Pueraria/Rehmannia glutinosa intake.

• Journal of Animal Science and Technology
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• v.47 no.4
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• pp.537-546
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• 2005

An experiment was conducted to determine the effect of supplementation of chlortetracycline (CTC,110ppm), copper sulfate(Cu, 125ppm) and two levels of probiotics(Prob I, 0.04%, Prob II, 0.1%), Bacillus licheniformis and Bacillus subtilis) on growth performance, nutrient digestibility and manure characteristics in growing pigs. A total of 50 pigs that averaged 20.78$\pm$0.35kg BW and 50$\pm$2.3d age were allocated in a randomized block design with two pigs per pen and 5 pens per treatment. Pigs and feeders were weighed 10-days interval for the 40-d trials to determine ADG, ADFI and feed:gain ratio(F:G). Average daily gain, feed intake, feed/gain and nutrient digestibility were not improved(P>0.10) by the supplementation of CTC, Cu and two levels of probiotics. There were significant(P<0.05) Cu effects on digestibility of crude protein, and probiotics effects on digestibilities on organic matter, crude protein and energy between first 20 days and subsequent 20 days. Fecal concentrations of copper were highly increased(P<0.001) by the copper supplementation. Total bacteria and coliform counts were not altered by the supplementation of CTC, Cu and two levels of probiotics. Ammonia emission from slurry, measured during first 3 weeks, was reduced (P<0.001) in pigs fed diet with 125 ppm copper from copper sulfate. Results indicate that CTC, Cu and Probiotics supplementation had a little or not positive effect on grower pig performance under sound environmental conditions. Further studies may be warranted to investigate the effects of dietary copper-either reduced or in combination with dietary ammonia control agents-on the ammonia emission characteristics of swine manure.

• Journal of Nutrition and Health
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• v.41 no.6
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• pp.530-538
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• 2008

This study was performed to assess the effects of beverage consumption on nutrient intake and anthropometric measurements. Beverage intake and dietary intake were measured by a beverage frequency and quantity questionnaire and three 24-hour dietary recalls in 160 elementary school students in the Gyeongnam area. The number of drinking moments per month was 93.5 and drinking amount of all beverages was 626.6 mL per day. The amount of milk intake was 253.8 ml per day, which was the highest in all types of beverage consumption, followed by juice(133.6 mL), and carbonated beverage(77.7 mL). The amount of sweetened beverage which was defined as all beverages except milk and soy milk was 359.7 mL per day. Energy intakes from all kinds of beverages and sweetened beverages accounted for 20.3% and for 10.5% of the daily energy intake, respectively. The contributions of sweetened beverage intake to the daily energy intake in girls accounted for 13.5%, whereas in boys it was 7.7% and significantly lower. The number of drinking moments and the contribution of sweetened beverage intake to daily energy intake were negatively correlated with protein, vitamin A and niacin intake, respectively. The consumption of milk and soybean milk combined showed a significant negative correlation with the body fat ratio(%) and it also negatively correlated with waist-hip girth ratio(WHR). However, the sweetened beverage intake was not related any of the anthropometric measurement. In conclusion, consumption of the sweetened beverage dropped the nutritional quality of diet. However it was not related with anthropometric measurements in elementary students.

• Korean Journal of Poultry Science
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• v.38 no.1
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• pp.29-35
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• 2011

An experiment was conducted to investigate the effects of dietary supplementation of a silicate mineral (D Lite) on growth performance, nutrient retention, carcass characteristics and meat quality in broiler chicks. A total of 640 broiler chicks were randomly allotted to four dietary treatments in a randomized complete block design. Each treatment had four replicates comprising 40 broilers in each replicate. Dietary treatments were as follows: control (0% D Lite), and diets supplemented with 0.15%, 0.30% and 0.45% of D Lite. The starter and finisher diets were fed from d 0 to 21 and d 21 to 35, respectively. Body weight gain during starter (p<0.05), finisher (p=0.057) and overall (p<0.001) experimental period and feed intake (p<0.05) during starter period decreased quadratically with increasing dietary D Lite levels. The birds fed 0.15% D Lite showed the greatest (p<0.05) overall weight gain, feed intake and retention of gross energy and calcium. There were no differences (p>0.05) in carcass characteristics, organ weights and breast meat composition. However, as the level of D Lite was increased in the diet there was a linear decrease in the TBARS values of breast meat (p<0.05) at d 12 of storage. These results suggest that D Lite supplementation at the level of 0.15% is shown to be effective in improving performance and nutrient retention in broilers.

• Korean Journal of Soil Science and Fertilizer
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• v.15 no.1
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• pp.2-15
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• 1982

In this report, a total domestic demand for major commercial fertilizer for crop production in Korea up to 1996 was estimated. The agricultural products and area for demand for both 1982 and 1986 was quoted from the estimate of the 5th Five-year Economic plan. And the demands estimated for 1991 and 1996 reflected possible changes of diet from cereal to meat and their indirect effects on the increase of cereal consumption. As the advanced countries followed, consequently, the demands for soybean, corn and other feed grains were expected to be increased as well as the land for growing those crops. 1. Total annual demands for nitrogen, phosphorus and potassium fertilizers were estimated 1,050,000M/T, 1,110,000M/T, 1,280,000M/T and 1,010,000M/T for the year 1982, 1986, 1991, and 1996 respectively. 2. It was assumed that there would be difficulties in self-sufficiency of grains at the cost of the maximum utilization of land and fertilizers in 1996. 3. It was clear that the increase of the productivity per unit area is possible by improving the conditions of arable land which could resulted a self-sufficiency of food in Korea. As a consequence, the demand for fertilizers at that time would exceed the level of estimates. 4. The recent decrease in demand for commercial fertilizers (currently estimated 850,000M/T) was due to an inadequate application of fertilizers for respective crop reqirement. This inadequacy should be checked and encouraged the consumptions of fertilizers to be increased by supporting the price of grain.

• Journal of Plant Biotechnology
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• v.38 no.2
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• pp.176-185
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• 2011

Rice is one of the most important crop in the world, in particular for food resources. With its small genome size of 383 Mb, the Oryza sativa is a model plant for genome research. Indeed, it's grain provides human with a source of carbohydrates and proteins. Rice grain has relatively low protein contents (around 8%) compared to other legume seeds (around 40%). Osborne classified seed proteins into water soluble albumin, salt soluble globulin, alcohol soluble prolamin and acidic/alkaline solution soluble glutelin. Glutelin and prolamin are the major storage proteins in rice. For the gene expression study of seed storage proteins, we analyzed 33,192 EST clones at immature stages in a rice cultivar (Oryza sativa L. cv. 'Ilpum'). Based on the expression analysis, we cloned 11 glutelin genes and figured out the 8 genes are located on Chromosome 2. The expression of glutelin genes appears to be about 28.2% of total level in immature seeds. Interestingly, glu-04 is duplicated as inverted sequences on the same chromosomes as far 4.5 kb. Our results indicate that glutelin genes, evolutionarily, were replicated on the chromosome and thus expressed as specific manners. In a whole protein composition analysis, glu05 (type B7) contains the highest lysin contents (4.51%) among the 11 rice glutelin genes. It will be an interesting future work to increase lysin contents by the gene overexpressor strategy with the aim of improved diet nutritionally fortified.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.63 no.2
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• pp.164-173
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• 2018

Elevated atmospheric carbon dioxide concentration ($CO_2$) is a major component of climate change, and this increase can be expected to continue into the crop and food security in the future. In this study, Soil-Plant-Atmosphere-Research (SPAR) chambers were used to examine the effect of elevated $CO_2$, temperature, and drought on the canopy architecture and concentration of macronutrients in potatoes (Solanum tuberosum L.). Drought stress treatments were imposed on potato plants 40 days after emergence. Under AT+2.8C700 (30-year average temperature + $2.8^{\circ}C$ at $700{\mu}mol\;mol^{-1}$ of $CO_2$), at maximum leaf area, elevated $CO_2$, and no drought stress, a significant increase was observed in both the aboveground biomass and tuber, and for the developmental stage. Even though $CO_2$ and temperature had increased, AT+2.8C700DS (30-year average temperature + $2.8^{\circ}C$ at $700{\mu}mol\;mol^{-1}$ of $CO_2$ under drought stress) under drought stress showed that the leaf area index (LAI) and dry weight were reduced by drought stress. At maturity, potatoes grown under $CO_2$ enrichment and no drought stress exhibited significantly lower concentrations of N and P in their leaves, and of N, P, and K in tubers under AT+2.8C700. In contrast, elevated $CO_2$ and drought stress tended to increase the tuber Mg concentration under AT+2.8C700DS. Plants grown in AT+2.8C700 had lower protein contents than plants grown under ATC450 (30-year average temperature at $400{\mu}mol\;mol^{-1}$ of $CO_2$). However, plants grown under AT+2.8C700 showed higher tuber bulking than those grown under AT+2.8C700DS. These findings suggest that the increase in $CO_2$ concentrations and drought events in the future are likely to decrease the macronutrients and protein concentrations in potatoes, which are important for the human diet.

• Applied Biological Chemistry
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• v.20 no.1
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• pp.43-57
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• 1977

In order to establish methods of potato processing for mixed cooking with rice as main diet, treatment for polyphenol oxidase inactivation in potato tissues, and safety in regard to sanitation were studied. The results were summarized as follows: 1. Cut potato granule which were blanched in boiling water are practical and best in color within $3{\sim}6\;minutes$ dipping. 2. Dipping treatment of potato granules in a saline solution is not pratical because of the resulting color and saline taste. 3. Dipping treatment of potato granules in a boiling saline solution is not practical because of residual salt. 4. Dipping treatment of potato granules in low concentration of hydrochloric acid solution is not effective in color changes but dipping treatment from 0.1N concentration for 4-10 minutes to 0.5N concentration for $1{\sim}10minutes$ shows good effects. 5. Dipping treatment in boiling hydrochloric acid solution of 0.04-0.068N concentration for 3-5 minutes dipping is practical for its resulting color. 6. Dipping treatments in 0.05% of $K_2S_20_5$ solution for $50{\sim}60\;minutes$ and in 0.06% for $40{\sim}60\;minutes$ and 0.065% for $10{\sim}60\;minutes$ shows a small palatability in color but its boiled potato granules shows good color 7. Treatments for any time in $0.01{\sim}0.03%$ of boiling $K_2K_20_5$ solution after predipping in its cold solution for longer time than 30minutes show effective in the color and palatability of the potato granules and their cooked ones. 8. From the view of food sanitation, dipping treatment of the potato granules in the solution of $K_2S_20_5$ is safe because of the few residual amount of $SO_2$. 9. All the methods of treatment that potato granules are dipped for any time at 0.01% of $K_2S_20_5$ solution are safe from the view of fond sanitation. At 0.03% of $K_2S_20_5$ solution, nothing but the methods of treatment that they are dipped within 30 minutes and boiled within 4 minutes are safe.

• Pediatric Gastroenterology, Hepatology & Nutrition
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• v.5 no.1
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• pp.26-32
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• 2002

Purpose: Constipation in childhood is a significant problem, however, understanding of its epidemiology has been limited in Korea. The aim of this study was to investigate the prevalence and risk factors of constipation in school-aged children, helping to manage and prevent it. Methods: This cross-sectional, descripsive observational prevalence study included children (2grade, 1st and 6th grade) who were attending two elementary schools in Gwangju. The prevalence of constipation, associated symptoms, and risk factors were evaluated by questionnaire. A child was considered to be constipated when he or she defecated two or less times per week. Results: The prevalence of constipation was 15.4%. There is no significant difference between both sexes and grades. Of the causes of constipation, there was statistically significant difference in exercise deficiency, frequent intake of convenience food, long-time watching TV, unbalanced diet and enuresis history between constipation and normal group. Especially, long-time watching TV, exercise deficiency, and enuresis history were risk factors for development of constipation. Only 27.1% of children with constipation were treated by laxative and enema. Conclusion: Even though the prevalence of constipation was high, more than half of them was not treated at all. We should endeavor to educate the children and their parents to prevent constipation by evaluating the feeding and behavioral habit completely, and treating them properly.

• FLOWER RESEARCH JOURNAL
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• v.16 no.3
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• pp.168-173
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• 2008

This study was conducted to determine biological characteristics of Acorus calamus L. angustatus by using aqueous, ethanol and methanol extracts from the leaves and the rhizomes of Acorus calamus L. angustatus plants. The highest total phenol contents were found in the extracts from the leaves of Acorus calamus L. angustatus ($68.4mg{\cdot}L^{-1}$) followed by rhizome ($49.3mg{\cdot}L^{-1}$). At $1,000mg{\cdot}L^{-1}$ the free radical scavenging activity of 1, 1-Diphenyl-2-picrylhydrazyl showed the highest activity in the extracts of the leaves and the rhizomes by 86.3% and 86.1%, respectively. Total flavonoid contents at $2,000mg{\cdot}L^{-1}$ extracts showed the greatest amount in the aqueous leaf extracts ($61.1mg{\cdot}L^{-1}$), and the ethanol rhizome extracts ($15.4mg{\cdot}L^{-1}$). Nitrite radical scavenging activity at $2,000mg{\cdot}L^{-1}$ was highest in the methanol extracts from leaves and rhizomes by 75.7% and 77.9%, respectively. Mushroom tyrosinase inhibition activity of leaves and rhizome from the Acorus calamus L. angustatus was very low, showing less than 1.3% regardless of the extract solvent, plant part, and concentration. These results indicated that methanol extracts from leaves exhibited higher biological activities than other extracts from rhizomes.