• KSBB Journal
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• v.24 no.2
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• pp.207-211
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• 2009

In this study, the sensing membranes for detection of pH and dissolved oxygen(DO) were prepared by immobilizing 6-aminofluorescein or ruthenium complex onto the sol-gel matrixes of GPTMS, MTMS, and TEOS and then recoated with the mixture of hydrophobic sol-gel and graphite for light insulation. The pH and DO sensing membranes recoated with the light insulation layer showed a higher sensitivity than those without light insulation layer. The sensing membranes were immobilized on the wells of 24-well microplate and used to monitor the fluorescence intensity for pH and DO in E.coli JM109 and P.pastoris X-33 fermentation processes. The change of the fluorescence intensity in the DO sensing membrane agreed with the growth patterns of microorganisms, that the membranes are valuable to monitor the DO in fermentation processes. In the case of pH monitoring, the fluorescence intensity has showed good correlation to the off-line pH data, that the pH membranes are valuable to monitor pH values in fermentations.

• Journal of the Korean Chemical Society
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• v.62 no.3
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• pp.181-186
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• 2018

Nitrosamines are the nitrosocompounds which are produced by nitrosation reactions of the secondary amine and nitrite, and has been found to be produced through the vulcanization process during the production of rubber products Recently, nitrosamines have been detected in rubber products and become a major topic. Condoms are disposable medical devices, so safety is important because they come into direct contact with the skin and mucous membranes. In this study, we developed an analytical method for nitrosamines in condoms by applying ISO 29941 method. The samples were eluted by distilled water, and target compounds were extracted by liquid-liquid extraction with dichloromethane. And then after concentrated, and quantitatively analyzed by LC-MS/MS. The accuracies of the analytical method were ranged from 85.8 to 108.7%, precisions were lower than 11.5%, and the detection limits were from 0.11 (NDPA and NDBA) to 0.48 (NPYR) ng/mL. Among the 31 condom samples, NDBA was detected from 2 cases by extraction of distilled water, and NDMA were detected from 1 case, NDEA from 4 cases and NDBA from 26 cases by extraction of artificial saliva (pH 4.5). The total amount of nitrosamines in all samples were less than $500{\mu}g/kg$.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.37 no.1
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• pp.43-59
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• 2017

This study presents the prediction methodology of debris flow occurrence areas using the SINMAP model. Former studies used a single calibration region applying some of the soil test results to predict debris flow occurrence in SINMAP model, which couldn't subdivide the soil properties for the target areas. On the other hands, a multi-calibration region using a detailed soil map and soil strength parameters (c, ${\phi}$) for each soil series to make up for limitation of former studies is proposed. In this process, soils with soil erodibility factor (K) are classified into three types: 1) gravel and gravelly soil. 2) sand and sandy soil, and 3) silt and clay. In addition, T/R estimation method using mean elevation of target area instead of T/R method using actual occurrence time is suggested in this study. The suggested method is applied to Seobyeok-1 ri area, Bonghwa-gun where debris flow occurred. As a result of comparison between two T/R estimation method, both T/R estimations are almost equal. Therefore, the suggested methodologies in this study will contribute to set up the national-wide mitigation plan against debris flow occurrence.

• Clinical and Experimental Reproductive Medicine
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• v.26 no.3
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• pp.419-432
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• 1999

Objective: To establish the evaluation system of the quality of oocytes on the basis of the incidence of cumulus cells apoptosis, to investigate the relationships beween the incidence of cumulus cells and the outcomes of IVF-ET. Method: Thirth-four cycles undergoing controlled ovarian hyperstimulation for IVF-ET with tubal infertility (23 cycles) or unexplained infertility (11 cycles) were included in this study. Cumulus cell masses surrounding mature oocyte and co-culture of embryos with autologous cumulus cells during IVF-ET process. The incidence of apoptosis in cumulus cells was assessed by apoptosis detection kit fluorescein. The effect of co-culture using cumulus cells and the incidence of cumulus cells apoptosis. Results: The results were as follows: 1. The incidence of apoptosis in cumulus cells markedly increased in patients aged 40 or over, while the fertilization rate was greatly decreased in those age group. 2. Apoptosis in cumulus cells was found in both the fertilized oocytes and unfertilized oocytes, but the incidence of apoptosis was higher in unfertilized oocytes. 3. There is no clear correlation between apoptosis in cumulus cells and the number of oocytes retrieved. However, the incidence of apoptosis was increased when the number of oocytes retrieved was 5 and fewer in comparison with $6{\sim}10$. 4. Embryo grade was significantly affected by the incidence of apoptosis in cumulus cells. 5. Pregnancy rate of IVF-ET per cycle was 29.4%, and the pregnant group had the higher fertilization rate and a significantly lower incidence of apoptosis in cumulus cells compared with the nonpregnant group. 6. When cumulus cells were used as helper cells in the co-culture of the embryo, in vitro activity of cumulus cells based on morphological change and proliferation did not influence the quality of embryo, but was closely associated with the implantation rate and pregnancy rate, which was enhanced when morphological changes and proliferation of cumulus cells was more active. 7. This difference in the outcome of IVF-ET according to in vitro activity of cumulus cells used for co-cultue was not associated with the incidence of apoptosis in cumulus cells; but rather had likely relations with the different secretion pattern of protein, which may be an embryo trophic factor by cumulus cells. Conclusion: These results suggest that the incidence of apoptosis in cumulus cells can be used in predicting oocyte qualities and the outcomes of IVF-ET. And the effect of co-culture largely depends on the in vitro activity of cumulus cells as well.

• Journal of Digital Convergence
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• v.11 no.10
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• pp.217-224
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• 2013

Due to the development of mobile technologies, the carrier (telecommunications) billing service market is rapidly growing. carrier (telecommunications) billing service allows users to make on-line purchases through mobile-billing. Users find this particularly convenient because the payment acts as a credit transaction. Furthermore, the system is commonly believed to be secure through its use of SMS (Short Message Service) authentication and a real-time transaction history to confirm the transaction. Unfortunately, there is a growing number of fraudulent transactions threaten the future of this system. The more well documented types of security breaches involves hackers intercepting the authentication process. By contaminating the device with security breaching applications, hackers can secretly make transactions without notifying users until the end of month phone bill. This study sheds light on the importance of this societal threat and suggests solutions. In particular, "secure" systems need to be more proactive in addressing the methods hackers use to make fraudulent transactions. Our research partially covers specific methods to prevent fraudulent transactions on carrier billing service providers' systems. We discuss about the proposed improvements such as complement of electronic payment systems, active promotion for fraudulent transactions enhanced monitoring, fraud detection and introduce a new authentication service. This research supports a future of secure communications billing services, which is essential to expanding new markets.

• Journal of the Institute of Electronics Engineers of Korea SD
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• v.47 no.2
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• pp.94-99
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• 2010

This paper presents a charge pump architecture for correcting the current mismatch due to the PVT variation. In general, the current mismatch of the charge pump should be minimized to improve the phase noise and spur performance of the PLL. In order to correct the current mismatch of the charge pump, the current difference is detected by the replica charge pump and fed back into the main charge pump. This scheme is very simple and guarantees the high accuracy compared with the prior works. Also, it shows a good dynamic performance because the mismatch is corrected continuously. It is implemented in 0.13um CMOS process and the die area is $100{\mu}m\;{\times}\;160{\mu}m$. The voltage swing is from 0.2V to 1V at supply voltage of 1.2V. The charging and discharging currents are $100{\mu}A$, respectively and the current mismatch due to the PVT variation is less than 1%.

• Journal of the Korean Chemical Society
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• v.55 no.1
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• pp.63-69
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• 2011

This work discusses the fabrication, development and potential response behaviors of $Ag^+$ ion-selective electrodes ($Ag^+$-ISE) based on N,N'-bis(2-hydroxybenzyl)-1,3-diaminopropane (L1) and N,N'-bis(2-hydroxybenzyl)-2,2-dimethyl-1,3-diaminopropane (L2) as carriers. The observations indicated that the resulting electrode based on L1 toward $Ag^+$ showed stable near-Nernst slope approaching 58.7 mV/dec and the optimum potential response characteristics in a linear range at least five orders of magnitude with a detection limit of $1.0{\times}10^{-6}M$. The proposed electrode displayed the preferential selectivity to $Ag^+$ against other tested cations. The excellent potential analytical characteristics could lead to the successful applications of silver assay in significant real samples, indicating that the proposed $Ag^+$-ISE showed a significant advancement of measurement capabilities. But for the electrode based on L2, the poor potential response characteristics were observed in total experiment process.

• The Journal of the Acoustical Society of Korea
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• v.22 no.5
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• pp.375-382
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• 2003

Lip-reading technique based on bimodal is to enhance speech recognition rate in noisy environment. It is most important to detect the correct lip-image. But it is hard to estimate stable performance in dynamic environment, because of many factors to deteriorate Lip-reading's performance. There are illumination change, speaker's pronunciation habit, versatility of lips shape and rotation or size change of lips etc. In this paper, we propose the IIR filtering in time-domain for the stable performance. It is very proper to remove the noise of speech, to enhance performance of recognition by digital filtering in time domain. While the lip-reading technique in whole lip image makes data massive, the Principal Component Analysis of pre-process allows to reduce the data quantify by detection of feature without loss of image information. For the observation performance of speech recognition using only image information, we made an experiment on recognition after choosing 22 words in available car service. We used Hidden Markov Model by speech recognition algorithm to compare this words' recognition performance. As a result, while the recognition rate of lip-reading using PCA is 64%, Time-domain filter applied to lip-reading enhances recognition rate of 72.4%.

• Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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• v.34 no.5
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• pp.503-514
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• 2016

Delineation of accurate object boundaries is crucial to provide reliable spatial information products such as digital topographic maps, building models, and spatial database. In LiDAR(Light Detection and Ranging) data, real boundaries of the buildings exist somewhere between outer-most points on the roofs and the closest points to the buildings among points on the ground. In most cases, areas of the building footprints represented by LiDAR points are smaller than actual size of the buildings because LiDAR points are located inside of the physical boundaries. Therefore, building boundaries determined by points on the roofs do not coincide with the actual footprints. This paper aims to estimate accurate boundaries that are close to the physical boundaries using airborne LiDAR data. The accurate boundaries are determined from the non-gridded original LiDAR data using initial boundaries extracted from the gridded data. The similar method implemented in this paper is also found in demarcation of the maritime boundary between two territories. The proposed method consists of determining initial boundaries with segmented LiDAR data, estimating accurate boundaries, and accuracy evaluation. In addition, extremely low density data was also utilized for verifying robustness of the method. Both simulation and real LiDAR data were used to demonstrate feasibility of the method. The results show that the proposed method is effective even though further refinement and improvement process could be required.

• Membrane Journal
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• v.15 no.4
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• pp.297-309
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• 2005

As a number of potential endocrine disrupting compounds (EDCs) are released into the environment, recently growing attention has been drawn to them. Therefore sensitive and reliable analytical methods are essential to monitor those compounds. In this study, complementary CC-MS and LC-MS were employed to analyze the endocrine disrupters, and the results of two methods were compared for di(2-ethylhexyl)phthalate (DEHP), benzylbutylphthalate (BBP), pentachlorophenol (PCP), and 4,4'-Isopropylidenediphenol (Bisphenol-A, or BPA). The results indicate that it was possible to lower the detection limits of EDCs by LC-MS. Also, LC-MS enabled to identify the EDCs as almost intact molecules. Furthermore, this study presented a nanofiltration membrane (MWCO 250) and a ultrafiltration membrane (MWCO 1,000) filtration system as methods far removing EDCs from drinking water containing $\gamma$-BHC, p,p'-DDE, BBP, p,p'-DDT, DEHP, PCP, and BPA. Cross-flow type nanofiltrations showed $100\%$ removal of EDCs, and the result implies that MWCO 250 nanofilter was sufficient for treatment of EDCs. The ratio of permeate flux to mass transfer coefficient of nanofiltration, high flux ultrafiltration, and low flux ultrafiltration with ultrapure water were 0.67, 3.4, and 0.44, respectively. It was found that nanofiltration and low flux ultrafiltration were operated at a diffusion dominant condition, and the high flux ultrafiltration was operated at a convection dominant condition. Furthermore, a diffusion dominant process attained reasonable rejection of EDCs. The removal in the ultrafiltration was depending on the molecular weight of an EDC, and the filtration was governed by diffusion-dominant hydrodynamic conditions.