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Monitoring of pH and dissolved oxygen in microorganism fermentation processes using a 24-well microplate  

Kim, Sun-Yong (School of Applied Chemical Engineering, Chonnam National University)
Kim, Chun-Kwang (Department of Material and Biochemical Engineering, Chonnam National University)
Sohn, Ok-Jae (Department of Material and Biochemical Engineering, Chonnam National University)
Rhee, Jong-Il (School of Applied Chemical Engineering, Chonnam National University)
Publication Information
KSBB Journal / v.24, no.2, 2009 , pp. 207-211 More about this Journal
Abstract
In this study, the sensing membranes for detection of pH and dissolved oxygen(DO) were prepared by immobilizing 6-aminofluorescein or ruthenium complex onto the sol-gel matrixes of GPTMS, MTMS, and TEOS and then recoated with the mixture of hydrophobic sol-gel and graphite for light insulation. The pH and DO sensing membranes recoated with the light insulation layer showed a higher sensitivity than those without light insulation layer. The sensing membranes were immobilized on the wells of 24-well microplate and used to monitor the fluorescence intensity for pH and DO in E.coli JM109 and P.pastoris X-33 fermentation processes. The change of the fluorescence intensity in the DO sensing membrane agreed with the growth patterns of microorganisms, that the membranes are valuable to monitor the DO in fermentation processes. In the case of pH monitoring, the fluorescence intensity has showed good correlation to the off-line pH data, that the pH membranes are valuable to monitor pH values in fermentations.
Keywords
monitoring; pH; dissolved oxygen; sol-gel; fermentation process;
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