• 대한의생명과학회지
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• 제14권2호
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• pp.115-118
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• 2008

Salmonella typhi is frequent causes of foodborne illness and its detection is important for monitoring disease progression. In this study, by using general PCR and novel LAMP (Loop Mediated Isothermal Amplification) assay, we evaluated the usefulness of LAMP assay for detection of Salmonella typhi. In this LAMP assay, forward inner primer (FIP) and back inner primer (BIP) was specially designed for recognizing target invA gene. Target DNA was amplified and visualized as ladder-like pattern of bands on agarose gel within 60 min under isothermal conditions at $65^{\circ}C$. When the sensitivity and reproducibility of LAMP were compared to general PCR, there was no difference of reproducibility but sensitivity of LAMP assay was more efficient than PCR (the detection limit of LAMP assay was 30 fg, while the PCR assay was 3 pg). These results indicate that the LAMP assay is a potential and valuable means for detection of Salmonella typhi, especially for its rapidity, simplicity and low cost.

• Journal of Biosystems Engineering
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• 제31권2호
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• pp.128-134
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• 2006

Frequent outbreaks of foodborne illness demand the need for rapid and sensitive methods for detection of these pathogens. Recent development of biosensor technology has a great potential to meet the need for rapid and sensitive pathogens detection from foods. An antibody-based fiber-optic biosensor and an automated reagents supply system to detect Listeria monocytogenes were developed. The biosensor for detection of Listeria monocytogenes in PBS and bacteria spiked food samples was evaluated. The automated reagents supply system eliminated cumbersome sample and detection antibody injection procedures that had been done manually. The biosensor could detect $10^4$ cfu/ml of Listeria monocytogenes in PBS. By using the fiber-optic biosensor, $2x10^8$ cfu/ml of Listeria monocytogenes in the food samples were detectable.

• Bulletin of the Korean Chemical Society
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• 제35권8호
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• pp.2400-2402
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• 2014

Cu (II) detection is of great importance owing to its significant function in various biological processes. In this report, we developed a novel coumarin-based chemosensor bearing the salicylaldimine unit (2) for $Cu^{2+}$ selective detection. The results from fluorescence spectra demonstrated that the sensor could selectively recognize $Cu^{2+}$ over other metal cations and the detection limit is as low as $0.2{\mu}M$. Moreover, the confocal fluorescence imaging in HepG2 cells illustrated its potential for biological applications.

• Asian Pacific Journal of Cancer Prevention
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• 제13권5호
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• pp.1723-1726
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• 2012

Objective: To summarize and evaluate various urinary markers for early detection, diagnosis and follow-up of human bladder cancer. Methods: A MEDLINE and PUBMED search of the latest literature on urinary markers for bladder cancer was performed. We reviewed these published reports and made a critical analysis. Results: Most urinary markers tend to be less specific than cytology, yielding more false-positive results, but demonstrating an advantage in terms of sensitivity, especially for detecting low grade, superficial tumors. Some tumor markers appear to be good candidates for early detection, diagnosis, and follow-up of human bladder cancer. Conclusion: A number of urinary markers are currently available that appear to be a applicable for clinical detection, diagnosis, and follow-up of bladder cancer. However, further studies are required to determine their accuracy and widespread applicability.

• 디지털산업정보학회논문지
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• 제14권3호
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• pp.39-44
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• 2018

Communication in real-time distributed system should have high reliability. To develop group communication Protocol with high reliability, potential fault should be known and when fault occurs, it should be detected and a necessary action should be taken. Existing detection method by Ack and Time-out is not proper for real time system due to load to Ack which is not received. Therefore, group communication messages from real-time distributed processing systems should be communicated to all receiving processors or ignored by the message itself. This paper can make be sure of transmission of reliable message and deadline by suggesting and experimenting fault detection technique applicable in the real time distributed system based on ring, and analyzing its results. The experiment showed that the shorter the cycle of the heartbeat signal, the shorter the time to propagate the fault detection, which is the time for other nodes to detect the failure of the node.

• 한국수산과학회지
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• 제47권6호
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• pp.740-744
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• 2014

In this study, the ${\beta}$-lactamase (VPA0477) gene was used as a new target for the PCR-based detection of Vibrio parahaemolyticus. Primers specific for the ${\beta}$-lactamase (VPA0477) gene of V. parahaemolyticus, were designed and incorporated into a PCR-based assay. The assay was able to specifically detect all of the 191 V. parahaemolyticus strains tested, but did not result in amplification of 39 other Vibrio spp. and non-Vibrio spp. strains tested. The detection limit of the assay was 10 CFU of V. parahaemolyticus RIMD2210633 from pure culture broth. The ${\beta}$-lactamase (VPA0477) gene-based assay developed in this study was sensitive and specific, and has great potential for the accurate detection and identification of V. parahaemolyticus in seawater or seafood samples.

• Bulletin of the Korean Chemical Society
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• 제15권12호
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• pp.1038-1042
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• 1994

The amperometric responses of thiourea were studied in 0.1 M NaOH by flow injection analysis. D. C. amperometric and pulsed amperometric detection methods were applied for the determination of thiourea at novel metal electrodes such as Pt and Au. Triple-step potential waveforms were adopted in the pulsed amperometric detection. With an optimized pulsed waveform, the current for the oxidation of thiourea was examined with the variation of flow rate of carrier solution and with the change in the amount of sample injected. Gold working electrode turned out to be better in sensitivity and signal to noise ratio than Pt electrode in the pulsed amperometric detection of thiourea. Detection limit is estimated to be 5.33 ${\times}$ 10$^{-5}$ M with this detection method.

• 한국산업정보학회논문지
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• 제20권2호
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• pp.73-83
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• 2015

본 논문에서는 순차적 결함 검출 방법을 이용하여 TFT-LCD의 결함 영역(Blob)을 효과적으로 검출하는 알고리즘을 제안한다. 먼저 결함과 배경 간의 휘도 차를 이용하여 영상의 각 화소들에 대한 결함 확률을 판단하고, 결함 확률에 따른 순차적 결함 검출 방법을 이용하여 결함 후보 화소를 검출한다. 여기서 결함 확률이란 결함 후보 화소가 검출된 단계에 따라 결함 영역에 포함될 가능성을 나타내다. 형태학 연산을 적용함으로써 검출된 후보 화소들을 후보 결함 영역으로 형성하고, 각 후보 결함 영역에 대한 결함 가능성을 계산하여 결함 영역을 검출한다. 모의 TFT-LCD 영상을 생성하여 제안 방법의 타당성을 검증하고, 실제 TFT-LCD 영상에 적용함으로서 제안 알고리즘의 우수한 결함 검출 성능을 확인하였다.

• Journal of Microbiology and Biotechnology
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• 제30권3호
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• pp.459-468
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• 2020

This study established a new polymerase spiral reaction (PSR) that combines with reverse transcription reactions for HCV detection targeting 5'UTR gene. To avoid cross-contamination of aerosols, an isothermal amplification tube (IAT), as a separate containment control, was used to judge the result. After optimizing the RT-PSR reaction system, its effectiveness and specificity were tested against 15 different virus strains which included 8 that were HCV positive and 7 as non-HCV controls. The results showed that the RT-PSR assay effectively detected all 8 HCV strains, and no false positives were found among the 7 non-HCV strains. The detection limit of our RT-PSR assay is comparable to the real-time RT-PCR, but is more sensitive than the RT-LAMP. The established RT-PSR assay was further evaluated for detection of HCV in clinical blood samples, and the resulting 80.25% detection rate demonstrated better or similar effectiveness compared to the RT-LAMP (79.63%) and real-time RT-PCR (80.25%). Overall, the results showed that the RT-PSR assay offers high specificity and sensitivity for HCV detection with great potential for screening HCV in clinical blood samples.

• 한국치위생학회지
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• 제14권5호
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• pp.609-615
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• 2014

Objectives : In most developed countries there has been a substantial decrease in the prevalence of dental caries in children of 12 years old over the past 40 years. This change in epidemiology highlights the importance of preventive management in dentistry. For successful management of early caries lesions, it is essential to detect and assess the pathological changes of dental caries. Therefore, the purpose of this study was to investigate the overview of International Caries Detection and Assessment System(ICDAS) criteria for the detection and assessment of early caries lesions by literature reviews. Methods : The author reviewed the MEDLINE search by the key words of 'caries assessment', 'caries detection', 'caries measurement', and 'ICDAS'. Results : This literature review provided an overview of International Caries Detection and Assessment System(ICDAS) criteria for the detection and assessment of early caries lesions. This article showed the principles, concept, and primary caries detection criteria of the ICDAS. The ICDAS can macroscopically measure the enamel surface changes and potential histological depth of caries lesions by the surface characteristics of the teeth. The ICDAS criteria allow researchers and clinicians to choose the stage of dental caries and characteristics for assessment. Conclusions : ICDAS can be an adjunct method to clinical decision making and preventive treatment planning.