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http://dx.doi.org/10.5307/JBE.2006.31.2.128

Development of a Fiber-Optic Biosensor for the Detection of Listeria monocytogenes  

Kim G. (National Institute of Agricultural Engineering, RDA)
Choi K.H. (National Institute of Agricultural Engineering, RDA)
Publication Information
Journal of Biosystems Engineering / v.31, no.2, 2006 , pp. 128-134 More about this Journal
Abstract
Frequent outbreaks of foodborne illness demand the need for rapid and sensitive methods for detection of these pathogens. Recent development of biosensor technology has a great potential to meet the need for rapid and sensitive pathogens detection from foods. An antibody-based fiber-optic biosensor and an automated reagents supply system to detect Listeria monocytogenes were developed. The biosensor for detection of Listeria monocytogenes in PBS and bacteria spiked food samples was evaluated. The automated reagents supply system eliminated cumbersome sample and detection antibody injection procedures that had been done manually. The biosensor could detect $10^4$ cfu/ml of Listeria monocytogenes in PBS. By using the fiber-optic biosensor, $2x10^8$ cfu/ml of Listeria monocytogenes in the food samples were detectable.
Keywords
Fiber-optic biosensor; Listeria monocytogenes; Food safety;
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1 Anderson, G.P., K.D. King, D.S. Cuttino, J.P. Whelan, F.S. Ligler, J.F. MacKrell, C.S. Bovais, D.K. Indyke, R.J. Foch. 1999. Biological agent detection with the use of an airborne biosensor. Field Analytical Chemistry and Technology 3(4-5): 307-314   DOI
2 Bhunia, A.K., T. Geng, A. Lathrop, A. Valadez, M.T. Morgan. 2004. Optical immunosensors for detection of Listeria monocytogenes and Salmonella Enteritidis from food. Proceedings of SPIE 5271: 1-6
3 CDC. 2002. Listeriosis outbreak investigation, CDC Press Release
4 Tims, T.B., S.S. Dickey, D.R. Demarco, D.V. Lim. 2001. Detection of low levels of Listeria monocytogenes within 20 hours using an evanescent wave biosensor. American Clinical Laboratory. Sep.: 28-29
5 DeMarco, D.R., E.W. Saaski, D.A. McCrae, D.V. Lim. 1999. Rapid detection of Escherichia coli O157:H7 in ground beef using a fiber-optic biosensor. Journal of Food Protection 62(7): 711-716   DOI
6 Donaldson, K.A., M.F. Kramer, and D.V. Lim. 2004. A rapid detection method for Vaccinia virus, the surrogate for smallpox virus. Biosensors and Bioelectronics 20: 322-327   DOI   ScienceOn
7 Tims, T.B. and D.V. Lim. 2003. Confirmation of viable E. coli O157:H7 by enrichment and PCR after rapid biosensor detection. Journal of Microbiological Methods 55: 141-147   DOI   ScienceOn
8 Marazuela, M.D. and M.C. Moreno-Bondi. 2002. Fiber-optic biosensors-an overview. Anal. Bioanal. Chem. 372: 664-682   DOI   ScienceOn