• Title/Summary/Keyword: Derived Contents

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Transgenic Lettuce Expressing Chalcone Isomerase Gene of Chinese Cabbage Increased Levels of Flavonoids and Polyphenols

  • Han, Eun-Hyang;Lee, Ji-Sun;Lee, Jae-Woong;Chung, In-Sik;Lee, Youn-Hyung
    • Horticultural Science & Technology
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    • v.29 no.5
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    • pp.467-473
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    • 2011
  • Flavonoid are large group of the polyphenolic compounds which are distinguished by an aromatic or phenolic ring structure and the phenolic compounds are induced by microbial infection, ultraviolet radiation, temperature and chemical stress. They are known for their antioxidant activity, anti-allergic, anti-inflammatory, anti-microbial and anti-cancer activities. In this study, changes in flavonoid content were investigated using heterologous chalcone isomerase (CHI) expression system. Also, phenolic compounds level was measured to examine the relation between flavonoids and phenols contents. Explants of lettuce (Lactuca sativa L.) were transformed with Agrobacterium tumefaciens LBA 4404 strain containing pFLH-CHI (derived from pPZP2Ha3) vector constructed with CHI gene from Brassica rapa. The putative transgenic plants were confirmed by genomic DNA PCR analysis. Also the transcription levels of the gene were analyzed by semi-quantitative RT-PCR with gene specific primers. The total flavonoid contents were increased at $T_0$ and $T_1$ generations over 1.4 and 4.0 fold, respectively. Total phenol contents also increased at $T_1$ generation. These results indicate that CHI gene plays an important role to regulate the accumulation of flavonoids and its component changes.

Quantitative comparison of acidic polysaccharides in the endosperm of two major varieties of rice

  • Hyun, Gyu Hwan;Lim, Dong Kyu;Kwon, Sung Won
    • Analytical Science and Technology
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    • v.30 no.4
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    • pp.205-212
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    • 2017
  • Rice endosperm, the portion that remains after milling, is the part of the rice seed that is primarily consumed as a source of nutrients. There have been many studies on polysaccharides, such as hemicellulose, cellulose, and pectins, derived from the cell walls of various plant groups. It has been reported that the acidic polysaccharide fractions, which contain water-soluble pectins that have been shown to have pharmacological effects in vivo and in vitro, have common chemical structures that include galacturonic acid polymers, rhamnose, arabinose, and galactose. However, few studies have been conducted on the acidic polysaccharides contained in the endosperm of rice. In this study, we quantitatively compared the differences in the acidic polysaccharide contents from samples from two of the main varieties of rice consumed as staple foods, japonica and indica, using a colorimetric method. Rice samples were collected from 39 different regions in Korea, China, Thailand and Vietnam. Acidic polysaccharide fractions were obtained by precipitation of the alcohol-insoluble residue (AIR) and enzyme treatment of each sample. The total amount of carbohydrates and uronic acid in each acidic polysaccharide fraction were measured using the phenol-sulfuric acid method and the carbazole-sulfuric acid method, respectively. The differences in the total polysaccharide contents in the acidic polysaccharide fractions were not statistically significant (p = 0.07), but the uronic acid contents were significantly different between the two groups (p = 0.04).

The study on derived problems and improvement analysis from tycoon type mobile game (타이쿤 모바일 게임의 문제점 도출 및 개선 방안에 대한 연구)

  • Park, Hong;Park, Jeong-Hyeon;Park, Jin-Wan
    • Proceedings of the Korea Contents Association Conference
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    • 2006.05a
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    • pp.181-184
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    • 2006
  • With the game contents which is suitable in quality of the interface which is restricted and the mobile game which is restricted at game playing time the tycoon game raises its head and with one style human nature it is receiving. But like this popularity it is the actual condition which is being pushed out wild because of game the game characteristic come into the market without with character little by little. It examines the trend and a production process and a composition of the tycoon mobile game which is immanent the game characteristic which is considerable from the research which it sees consequently and it currently problem point a tycoon mobile game with character and escape a improvement program and to sleep it presents it does.

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The Effect of L-Ascorbic Acid on the Formation of Immature Crosslink in Bone Collagen in vitro (비타민 C가 in vitro 계에서 콜라겐 미숙가교 생성에 미치는 영향)

  • 김미향
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.28 no.6
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    • pp.1332-1338
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    • 1999
  • Intermolecular collagen cross links stabilize collagen fibrils and are necessary for normal tensile strength in collagen fibrils. Once the fibrils are aligned, hydroxyllysine, hydroxylysine derived aldehyde modified enzymatically, reacts with hydroxylysine to form the dehydrodihydroxylysinonorleucine (DHLNL), an immature crosslink. Pyridinoline, one of matured cross links is presumably formed nonenzymatically through condensation of DHLNL and hydroxylysine residue. It is widely distributed in hard connective tissues such as cartilage, bone and tendon. L ascorbic acid(AsA) is well known to be required for the enzymatic hydroxylation of proline and lysine in collagen fibrils. The purpose of this study is to clarify the role of AsA on the biosynthesis of DHLNL in vitro. We examined the effect of AsA on the formation of hydroxylysine and DHLNL in collagen. Pyridinoline and DHLNL were measured as a function of time. The contents of DHLNL was increased, reached maximum within 2 hr and was held until 24 hr, then it decreased slowly. On the contrary, pyridinoline increased gradually after 24 hr and continued to increase for 2 weeks. Moreover, the contents of DHLNL remarkably decreased at 60 min after incubation, the contents of DHLNL was decreased by addition of AsA or dehydroascorbic acid(DHA). These results suggest that the supplementation of AsA causes decrease in DHLNL formation and pyridinoline formed by nonenzymatic reaction of DHLNL.

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A Study on the Development of Teacher Librarians' Teaching Practice Program in Korea (예비사서교사의 교육실습 프로그램 개발에 관한 연구)

  • Kim, Sung-Jun
    • Journal of Korean Library and Information Science Society
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    • v.42 no.2
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    • pp.193-213
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    • 2011
  • Teacher librarians' teaching practice is a major training course to strengthen their expertise. The purpose of this study is to propose more systematic program for effective teaching practice of teacher librarian in Korea. For this, the course contents of teaching practice were derived from the teacher librarians' expertise standards published by Korea Institute for Curriculum and Evaluation. And the survey was carried out to decide which contents are regarded as more important or less. According to four selection criteria and the result of survey, 13 contents were selected. After all, this study proposed teacher librarians' teaching practice program consisted of practice objective, method of guidance, practice material and attentive point.

The problems and improvement directions of certificate in-service training for "Environment" subjects ("환경"과 부전공 자격연수의 문제점과 개선방향)

  • 김인호;이선경
    • Hwankyungkyoyuk
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    • v.8 no.1
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    • pp.138-150
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    • 1995
  • The quality of environmental education in school depends on the quality of environment teacher, who develops his/her ability through avalible courses such as in-service training and communication et al. This study was performed with the questionnaire to 179 teachers who participated in certificate in-service training for [Environment] subjects on winter of '95 and summer of '96. The questionnaire included 28 items, which could be categorized 5 parts : the environmental consciousness of teachers, their awareness of environmental education in schools, the reason of participation, the limits and requirements of learning field, avalible teaching and learning methods. The improvements and suggestions derived from this study are summarized as follows: First, it is necessary to differentiate in-service training concerning environment, because the motive of teacher's participation is diverse and is not always suitable to the purpose of certificate in-service training for [Environment] subjects. Second, the teaching contents and programs of certificate in-service training for [Environment] subjects have to be various according to the characteristics and major subjects of teachers. Third, it deserves special emphasis that the field trips and survey for the experiential study in field is supplemented in the teaching contents and programs of certificate in-service training for [Environment] subjects. Also, it is necessary to strengthen the environmental ethics and philosophy in the teaching contents and programs.

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Activated Carbon Performance for the Treatment of Diesel-Derived Polycyclic Aromatic Hydrocarbons

  • Choi, Yongju;Luthy, Richard G.
    • Ecology and Resilient Infrastructure
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    • v.2 no.2
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    • pp.177-184
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    • 2015
  • This study assessed the performance of activated carbon (AC) amendment to treat polycyclic aromatic hydrocarbons (PAHs) including both parent- and alkylated-moieties in sediment impacted by diesel. A field-collected, diesel-impacted sediment with a NAPL content of 1% was used for the study. No. 2 diesel fuel is weathered by heating at $70^{\circ}C$ for 4 days to obtain a weathered diesel sample having C3-naphthalenes to C2-phenanthrenes/anthracenes (N2/P3) ratio similar to the original sediment. The sediment samples spiked with the weathered diesel to obtain non-aqueous phase liquid (NAPL) contents of 1, 5 and 10% were contacted with AC with a dose of 5% as sediment dry weight for 1 month. By the AC-sediment contact, the freely-dissolved equilibrium concentrations were substantially reduced. Even for sediment with 10% NAPL content, the reductions in the freely-dissolved concentrations were 92% and 75% for total parent-and alkylated-PAHs, respectively. The effect of NAPL contents on the performance of AC was negligible for parent-PAHs, while for alkylated-PAHs, a slightly reduced AC performance was observed. The results suggest that the AC amendment can be an effective option for the treatment of petroleum-impacted sediment with relatively high NAPL contents.

Analysis of the Contents of Hanbok in the 「Home Life and Safety」 section of the High School Technical Family Textbook: Content Analysis and Text Mining Techniques are utilized (고등학교 기술·가정 교과서 「가정생활과 안전」 영역의 한복 내용 분석)

  • Shim, Joon Young;Baek, Min Kyung
    • Human Ecology Research
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    • v.59 no.2
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    • pp.261-273
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    • 2021
  • This study is not just a meaning of costume but a function of culture and includes addresses the associated emotions. As the interest of youths has increased recently, the importance of traditional costume education has been growing. Therefore, this study aims to analyze the contents of Hanbok in the 2015 revised high school technology and home textbooks using content analysis techniques and text mining techniques. As a result of the study, first, the symbolic meaning and characteristics of Hanbok and the beauty of Hanbok were practiced in daily life, and the value was found through the excellence of Hanbok and the modernization of Hanbok was dealt with Second, most of the illustrations related to traditional costumes were presented in various ways, but there were some regrets due to lack of quantity and quality. Third, the words used to explain traditional costumes were used in the form of culture, excellence, tradition, modernity, harmony, succession, etc. except for the types of clothing. Therefore, the results and discussions derived from this study are expected to help the textbooks to be efficiently selected and used in the field of the front line school along with the correct understanding of traditional culture in the process of selecting traditional culture contents and illustrations.

The Study on Design an Ontology for Korean Food Information (한식정보 활용을 위한 온톨로지 설계에 관한 연구)

  • Yu, Ha-Gyeong;Park, Ok Nam
    • The Journal of the Korea Contents Association
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    • v.19 no.2
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    • pp.147-158
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    • 2019
  • The Korean food, which has been attracting attention only as a unique culture of Korea, has become popular in the world market by being used as a material of Korean Wave and me-media. The systematic organization of knowledge for recipes and related information can highlight the value of Korean food. It will serve as a basis for improving the reusability of Korean food contents through expanded and limited search and effective browsing. This study purports to design an ontology for establishing Korean food knowledge structure. Ontology modeling is based on OWL. Vocabularies of Korean food were examined based on 32 volumes of Korean food information, and data elements were extracted by analyzing five domains and applications. As a result, the study derived classes and properties, and proposed an indexing example.