• Microbiology and Biotechnology Letters
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• v.22 no.5
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• pp.467-476
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• 1994

Lactobacillus casei HY 2782, prophage cured strain was characterized to be stable as much as L casei YIT 9018, parent strain. By southern hybridization, it was confirmed that the temperate phage was incorporated in chromosomal DNA of L. casei YIT 9018 as a prophage. It was also proved that the prophage was cured from chromosomal DNA of L casei HY 2782. The growth rate, lactic acid producing ability, carbohydrates fermentation, and enzymatic activity of L. casei HY 2782 were found to be similar to those of L. casei YIT 9018. When L casei HY 2782 was used as a host, the multiplicity of infection (M.O.I.) of the temperate phage for L. casei HY 2782 was 1.0~5.0. Restriction enzyme analysis of pLC90 plasmid from L. casei HY 2782 was shown that the size was an approximately 68.22 kb. The plasmid profiles, genomic DNA patterns, and cellular fatty acids composition of L. casei HY 2782 were similar to those of L casei YIT 9018. And the major fatty acids composition of these strains were C$_{14;0}$,C$_{16;1}$, C$_{16;0}$, C$_{18;1}$ and C$_{19;cyclo-}$ 10 sets of arbitrary primer in the PCR were screened to find differentiation against two strains of L. casei. Among them, b$_{5}-1/17-1 primer was produced an approximately 1.3 kb DNA band of only L casei YIT 9018. And b$_{5}-2/17-2 primer was produced an approximately 1.0 kb DNA band of only L casei HY 2782.

• Journal of Biomedical Engineering Research
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• v.31 no.4
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• pp.328-334
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• 2010

Over the past few years, a considerable number of methods have been proposed and applied for the classification of photoplethysmography (PPG). Most of the previous studies, however, focused on the qualitative description of the pulse type according to specific disease and thus provided ambiguous criteria to interpreters. In order to screen out this problem, we present a quantitative method for the pulse type classification including the second derivative of photoplethysmography (SDPTG). In the PPG signal, we have classified the signal as 4 types using the position and the presence of the dicrotic wave. In addition, we have categorized the SDPTG signal as 7 types using the position and the presence of "c" and "d" wave and the sign of "c" wave. In order to check the efficacy of the proposed pulse type classification rule, we collected pulse signals from 155 subjects with different ages and sex. From the correlation analysis, Class 1(p<0.01) and Class 2(p<0.01) in the PPG signal are significantly correlated with ages. In a similar manner Class A(p<0.01), Class C(p<0.05), Class D(p<0.01), and Class F(p<0.01) in the SDPTG signal are considerably correlated with the ages. From these observations, and some earlier ones [4], [5], we can conclude that since the newly proposed method has objectivity and clarity in pulse type classification, this method can be used as an alternative of previous classification rules including similar age-related characteristics.

• Tribology and Lubricants
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• v.32 no.5
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• pp.141-146
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• 2016

The diketopyrrolopyrrole (DPP) pigment is a bicyclic 8-π-electron system containing two lactam units. Typical DPP derivative pigments have melting points of over 350°C and very low solubility in most solvents, and show absorption in the visible region with a molar extinction coefficient of 33,000 dm²mol⁻¹ and strong photoluminescence with maxima in the range 500–600 nm. X-ray structure analyses of DPP show that the whole molecule is almost in one plane. The phenyl rings are twisted out of the heterocyclic plane and the intermolecular hydrogen bonding between neighboring lactam NH and carbonyl units influences the structure of the DPP pigment in the solid state. In this study, mono-N-alkylation and mono-N-arylation were undertaken for Pigment Red 264 or Pigment Orange 73 with alkyl halide and aryl halide, respectively, in the presence of sodium tert-butoxide as a base catalyst to improve the solubility of DPP pigments and their application as CO₂ indicators. The synthetic yield was in the range 11–88%. The indicator dyes are highly soluble in organic solvents and shows pH-dependent absorption (λ_max 501 and 572 nm for the protonated and deprotonated forms, respectively) and emission (λ_max 524 and 605 nm for the protonated and deprotonated forms, respectively) spectra. The mono-N-alkylated and mono-N-arylated DPP pigment was identified by ¹H-NMR (¹H-Nuclear Magnetic Resonance Spectrometer), FT-IR (Fourier Transform Infrared Spectroscopy), and MS (Mass Spectrometry). According to the results of color and hue properties obtained by a color matching analyzer, the synthesized DPP pigment material can be used as a CO₂ indicator.

• Plant Biotechnology Reports
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• v.2 no.2
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• pp.123-131
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• 2008

Vitrification methods are convenient for cryopreserving plant specimens, as the specimens are plunged directly into liquid nitrogen (LN) from ambient temperatures. However, tissues and species with poor survival are still not uncommon. The development of vitrification solutions with high survival that cover a range of materials is important. We attempted to develop new vitrification solutions using bromegrass cells and found that VSL, comprising 20% (w/v) glycerol, 30% (w/v) ethylene glycol, 5% (w/v) sucrose, 10% (w/v) DMSO and 10 mM $CaCl_2$, gave the highest survival following cryopreservation, as determined by fluorescein diacetate staining. However, the cryopreserved cells showed little regrowth, for unknown reasons. To check its applicability, VSL was used to cryopreserve gentian axillary buds and the performance was compared with those of conventional vitrification solutions. Excised gentian stem segments with axillary buds (shoot apices) were two-step precultured with sucrose to induce osmotic tolerance prior to cryopreservation. Gentian axillary buds cryopreserved using VSL following the appropriate preculturing approach exhibited 78% survival (determined by the regrowth capacity), which was comparable to PVS2 and PVS1 and far better than PVS3. VSL had a wider optimal incubation time (20-45 min) than PVS2 and was more suitable for cryopreserving gentian buds. The optimal duration of the first step of the preculture was 7-11 days, and preculturing with sucrose and glucose gave a much higher survival than fructose and maltose. VSL was able to vitrify during cooling to LN temperatures, as glass transition and devitrification points were detected in the warming profiles from differential scanning calorimetry. VSL and its derivative, VSL+, seem to have the potential to be good alternatives to PVS2 for the cryopreservation of some materials, as exemplified by gentian buds.

• Korean Journal of Pharmacognosy
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• v.36 no.1 s.140
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• pp.44-49
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• 2005

The protective effects of the DWP-04 [DDB : selenium yeast: glutathione {31.1 : 6.8 : 62.1 (%, w/w)} against hepatotoxicity by carbon tetrachloride $(CCl_4)$ were studied in rats. The rats were intraperitoneally injected with $CCl_4$ (50% in com oil) at initial dose of 1 ml/kg followed by 0.5 ml/kg 3 times during 1 week. The DWP-04 (50, 100 or 200 mg/kg) or its vehicle was administered everyday before the start of $CCl_4$ injection for two weeks. $CCl_4$ induced hepatocelluar degeneration and necrosis, which led to a great increase in serum aminotransferase, alkaline phosphatase activity and serum lipid levels. It was found by biochemical analysis that $CCl_4$ treatment remarkably increased thiobarbituric acid reactive substances and physphatidylcholine hydroperoxide in hepatic tissues and induced antioxidant enzymes such as catalase and superoxide dismutase (SOD). Liver and serum lipids were significantly lower in rats fed on DWP-04 than in rats induced by $CCl_4$ only-treatment. These results suggested that the DWP-04 could be a promising candidate for the protection of liver injury based on the preventive effects against lipid peroxidation and serum biochemical parameters.

• Journal of Biosystems Engineering
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• v.31 no.3 s.116
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• pp.203-208
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• 2006

This study was conducted to improve the control performance of a current variable-rate controller for granular fertilizers. Simulation model was developed. Optimized proportional, integral and derivative gains were determined by simulation model using 2nd order PID gain learning algorithm, and these control gains were evaluated through the field tests. Important results of this study are as follows; 1. Principles of pre-existing variable-rate application of granular fertilizers were investigated. 2. Simulation model of a PID controller that could simulate the control system was developed by using Matlab/Simulink program. The program was to determine PID control coefficients through the simulation model and 2nd order PID gain learning algorithm. 3. PID control coefficients obtained from the simulation were applied to the developed model. When the step input was given, Maximum overshoot were 1.96%, rise time were 0.05 sec, settling time were 0.06 sec and steady state error were 0.21 % respectively. 4. The simulation model was verified through field tests. The errors of maximum overshoot were 10%, rise time were 0.11 sec, settling time were 0.40 sec and steady state error were 8% because of loads and noises. Rise time was decreased to one third of that of the pre-existing system. 5. If the speed of a fertilizing machine is $0.3{\sim}0.6\;m/s$ and the maximum rotation speed of a discharging roller is 64 rpm, rise time would be 0.26 sec and fertilizing machine would cover the distance of $0.07{\sim}0.15\;m$ with settling time of 0.4 sec, fertilizing machine would cover the distance of $0.12{\sim}0.24\;m$.

• Journal of Korean Society of Transportation
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• v.31 no.2
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• pp.33-44
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• 2013

The land use-transportation models typically have complicated model structure that is good for empirical execution but bad for theoretical probe. This complexity makes it very difficult to derive the first-order conditions for system optimization in tractable forms. Yu and Rhee (2011) and Rhee (2012) show how to simplify the derivative of the model's objective function with respect to policy variables in the computable general equilibrium model of land use and transportation. However, the travel demand in their model was fixed. This drawback fundamentally limits the applicability of their methodology in the planning field. We relax this restriction. Once this is done, we can employ the methodology developed in analyzing the impacts of various types of policy instruments in the models where land market is treated endogenously and transportation network is embedded.

• Korean Journal of Veterinary Research
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• v.56 no.2
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• pp.97-101
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• 2016

Emodin is an anthraquinone derivative from the roots of Rheum officinale Baill that possesses a variety of biological activities, including inhibition of $5{\alpha}$-reductase and prostaglandin D2. In this study, we investigated whether emodin promotes hair growth. After emodin was topically applied to the shaved dorsal skin of telogenic C57BL/6 N mice, the hair growth rate and morphological analysis were evaluated in dorsal skin for 15 days. After 13 days of treatment, minoxidil or emodin (0.01% or 0.1%)-treated groups showed remarkable regrowth of hairs relative to the vehicle control group. Scoring of the hair growth and rate of hair growth area for 15 days revealed that groups treated with minoxidil and 0.1% emodin were significantly higher than the vehicle control group. Histological examination revealed the emodin and minoxidil groups markedly recovered the number and morphology of hair follicles, including the subcutis depth, relative to the vehicle group. These results suggest that emodin has an excellent promoting effect in hair growth similar to that of minoxidil and might be useful for treatment of baldness or alopecia.

• Korean Journal of Food Science and Technology
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• v.44 no.2
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• pp.247-250
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• 2012

Raw milk samples (n=28) obtained from milk tanks in 3 dairy plants of different regions and commercial milks (n=100) were collected from six cities. These samples were analyzed for the level of aflatoxin $M_1$ contamination using immunoaffinity columns and high performance liquid chromatography coupled with fluorescent detectors. Confirmation of aflatoxin $M_1$ ($AFM_1$) identified in positive samples was based on the formation of the hemiacetal derivative ($AFM_{2a}$) after derivatization with trifluroacetic acid. The average concentrations of aflatoxin $M_1$ in the raw milks were 25.1 ng/kg, and those values in commercial milks were 29.8 ng/kg. The highest level of aflatoxin $M_1$ in milk was 72.7 ng/kg. These results showed that the contamination of aflatoxin $M_1$ in milks consumed in the Korea was quite low compared to the standard in Korea Food Code (aflatoxin $M_1$ 500 ng/kg).

• Journal of the Korea Institute of Information and Communication Engineering
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• v.15 no.10
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• pp.2217-2222
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• 2011

In this paper, the relationship of potential and charge distribution in channel for double gate(DG) MOSFET has been derived from Poisson's equation using Gaussian function. The relationship of subthreshold swing and oxide thickness has been investigated according to variables of doping distribution using Gaussian function, i.e. projected range and standard projected deviation, The analytical potential distribution model has been derived from Poisson's equation, and subthreshold swing has been obtained from this model for the change of oxide thickness. The subthreshold swing has been defined as the derivative of gate voltage to drain current and is theoretically minimum of 60 mS/dec, and very important factor in digital application. Those results of this potential model are compared with those of numerical simulation to verify this model. As a result, since potential model presented in this paper is good agreement with numerical model, the relationship of subthreshold swing and oxide thickness have been analyzed according to the shape of doping distribution.