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Preventive effect of a Schizandrin C derivative DDB-mixed preparation (DWP-04) against hepatotoxicity induced by Carbon Tetrachloride  

Lee, Jung-Hee (College of Pharmacy, Kyungsung University)
Chi, Sang-Cheol (College of Pharmacy, Sungkyunkwan University)
Kim, Seok-Hwan (Department of Food and Nutrition, Dong-A University)
Shin, Young-Ho (Dae Woo Pharmaceutical Ind. Co.)
Park, Hee-Juhn (Division of Applied plant Sciences, sangji University)
Choi, Jong-Won (College of Pharmacy, Kyungsung University)
Publication Information
Korean Journal of Pharmacognosy / v.36, no.1, 2005 , pp. 44-49 More about this Journal
Abstract
The protective effects of the DWP-04 [DDB : selenium yeast: glutathione {31.1 : 6.8 : 62.1 (%, w/w)} against hepatotoxicity by carbon tetrachloride $(CCl_4)$ were studied in rats. The rats were intraperitoneally injected with $CCl_4$ (50% in com oil) at initial dose of 1 ml/kg followed by 0.5 ml/kg 3 times during 1 week. The DWP-04 (50, 100 or 200 mg/kg) or its vehicle was administered everyday before the start of $CCl_4$ injection for two weeks. $CCl_4$ induced hepatocelluar degeneration and necrosis, which led to a great increase in serum aminotransferase, alkaline phosphatase activity and serum lipid levels. It was found by biochemical analysis that $CCl_4$ treatment remarkably increased thiobarbituric acid reactive substances and physphatidylcholine hydroperoxide in hepatic tissues and induced antioxidant enzymes such as catalase and superoxide dismutase (SOD). Liver and serum lipids were significantly lower in rats fed on DWP-04 than in rats induced by $CCl_4$ only-treatment. These results suggested that the DWP-04 could be a promising candidate for the protection of liver injury based on the preventive effects against lipid peroxidation and serum biochemical parameters.
Keywords
DWP-04; preventive effect; hepatotoxicity; catalase; superoxide dismutas; carbon tetrachloride;
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