• Title/Summary/Keyword: Derivative analysis

Search Result 826, Processing Time 0.034 seconds

Influence of UV-B Radiation on Photosynthesis, Growth and Pigmentation of Chondrus ocellatus (Rhodophyta) from Shallow Water

  • Taejun Han;Han, Young-Seok;Cho, Man-Gee;Park, Jin-Hee;Goo, Jae-Gun;Kang, Sung-Ho
    • Korean Journal of Environmental Biology
    • /
    • v.21 no.4
    • /
    • pp.368-376
    • /
    • 2003
  • The UV-B sensitivity was tested for the intertidal species Chondrus ocellatus from Korea, by measuring photosynthesis estimated as effective quantum yield ($\Phi_{PSII}$) of photosystem II (PS II), growth and content and composition of photosynthetic pigments and UV-absorbing pigments (UVAPs). The $$\Phi_{PSII}$ of the alga decreased with increasing time of exposure to UV-B radiation, followed by fast and nearly full recovery indicating dynamic photoinhibiton. Fresh weight-based growth and pigment contents of C. ocellatus were not seriously affected by UV-B radiation. A single broad peak at 327 nm was obtained from methanol extracts of C. ocellatus, and the absorbance peak increased with increasing UV. The single peak was resolved into three peaks (311, 330 and 336 nm) by the fourth -derivative, and quantitative change in response to UV-B radiation occurred only at 330 nm. High performance liquid chromatography (HPLC) analysis of purified extracts indicated that three MAAs (mycosporine-like amino acids) are present, asterina 330, palythine and shinorine. Field observations during three growing months showed that C. ocellatus exhibit the highest amount of UVAPs in May followed by July and little trace in September, coinciding with the species' phenology. In an ecological context, dynamic photoinhibition as well as accumulation of UVAPs may enable the shallow water red alga C. ocellatus to be well adapted to high UV-B environments.

Analysis of a Night Illuminance Distribution in School Buildings (학교 건축물의 야간 조도분포 분석)

  • 박동화;성낙진;신재화;이병기
    • The Proceedings of the Korean Institute of Illuminating and Electrical Installation Engineers
    • /
    • v.10 no.6
    • /
    • pp.48-53
    • /
    • 1996
  • In this paper, the illuminance of elementary, junior, and senior high school in Inchon was measured and analyzed to investigate their night illuminating circumstances(classrooms, science rooms, and laboratories). More than four schools were selected based on the years elapsed(l0, 20, and 30 years, etc) since the opening of the schools. It was shown that the average illuminance for the classroom and that for science room and laboratory have been improved significantly such that the former was 235.6[lx] (260[lx] for senior high school, 208[lx] for junior high school, 203[lx]) for elementary school) and the latter was 233.7[lx](248.8[lx] for senior high school, 216.4[lx] for junior high school, 207.8[lx] for elementary school). The uniformity of the illuminance was exceedingly worse than the recommended one(the average value for the classroom and that for sciense room and laboratory were 0.95 and 1.08, respectively). It was found that the maximum to minimum illuminance ratio with respect to illumination derivative(7.9 for classroom, 6.8 for scienceroom or laboratory) was very high for most school buildings and was much higher for the old ones. From the measured results, it was shown that the illuminance for the school buildings needs to be improved with regard to quality.

  • PDF

Experiment on Track-keeping Performance using Free Running Model Ship (모형 선박을 이용한 선박 침로유지 실험 연구)

  • Im, Nam-Kyun;Tran, Van-Luong
    • Journal of the Korean Society of Marine Environment & Safety
    • /
    • v.18 no.3
    • /
    • pp.221-226
    • /
    • 2012
  • This research presents an analysis of algorithm for ship track-keeping along a given trajectory. The maneuver of a free running model ship guiding through a simple path are presented. In order to solve the above problem, a desired trajectory is usually determined by GPS points in a pre-fixed place then these points are set in a pre-programmed navigation so that the ship would be automatically tracked. Proportional-Derivative(PD) control which is useful for fast response controllers was used in this program as a course keeping system. A high accuracy GPS receiver was installed on the model ship that could provide positions frequently, the system will compare and give out the remaining distance and heading to the target way-point. The results of ship auto track-keeping experiment will be explained in order to illustrate the adjustment in controlling parameters. These results can be utilized as a preliminary step to carry out the experiment of ship collision avoidance system and automatic berthing in the future.

Determination of Glutathione in Biological Samples by Ion-pairing HPLC/FLD (이온쌍 HPLC/FLD를 이용한 생체 시료중의 Glutathione 농도 분석)

  • Yoo, Jeong-Yeon;Lee, Kyoung-Ok;Shin, Ho-Sang
    • Analytical Science and Technology
    • /
    • v.12 no.1
    • /
    • pp.28-33
    • /
    • 1999
  • Glutathione(GSH) in biological samples was determined by high performance liquid chromatographic(HPLC) method with fluorescence detector(FLD) after monobromobimane(MBB) or 4-fluoro-7-sulfobenzofurazan(SBD-F) derivatization. The detection limit of $0.03{\mu}g/mL$ was obtained after MBB derivatization, derivative of MBB was about 200 times more sensitive than that of SBD-F. N-acetylcysteine was used as internal standard and tetrabutylammonium ion as counter ion for better separation. The determination by ion-pairing chromatography after MBB derivatization was characterized by linearity in the range between $0.08{\sim}8.33{\mu}g/mL$ with a good correlation coefficient of 0.998. By precision test appeared relative standard deviation at less than 5% at three different concentrations. This method can be used for the analysis of GSH in plasma and tissue.

  • PDF

Determination of Histamine by Reverse-Phase High Performance Liquid Chromatography with Fluorescence Detection (형광검출 역상 액체크로마토그래피에 의한 Histamine의 정량)

  • Ryu, Hee-Chun;Kim, Hyung-Ryong;Kim, Sang-Hyun;Kim, Dae-Ki;Lee, Young-Mi;Kim, Hyung-Min;An, Nyeon-Hyoung;Shin, Tae-Yong
    • Analytical Science and Technology
    • /
    • v.9 no.2
    • /
    • pp.198-202
    • /
    • 1996
  • A rapid and simple method for the determination of histamine by reverse-phase high performance liquid chromatography with fluorescence detection was established. 9-Fluorenylmethyl chloroformate(FMOC) was used as fluorescent derivative reagent. The optimum conditions for the derivatization such as pH, reaction time and the concentration of FMOC were investigated. Linearity of calibration curve was obtained between $0.1{\mu}g/ml$ and $0.5{\mu}g/ml$(r=0.992) and the limit of detection was $0.01{\mu}g/ml$.

  • PDF

Analysis of the Characteristics of a White OLED using the Newly Synthesized Blue Emitting Material nitro-DPVT by Varying the Doping Concentrations of Fluorescent Dye and the Thickness of the NPB Layer (신규 합성한 청색발광재료 nitro-DPVT를 사용한 백색 유기발광다이오드의 형광색소 도핑농도 및 NPB 층의 두께 변화에 따른 특성 분석)

  • Jeon, Hyeon-Sung;Cho, Jae-Young;Oh, Hwan-Sool;Yoon, Seok-Beom
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
    • /
    • v.19 no.4
    • /
    • pp.379-385
    • /
    • 2006
  • A stacked white organic light-emitting diode (OLED) having a blue/orange emitting layer was fabricated by synthesizing nitro-DPVT, a new derivative of the blue-emitting material DPVBi on the market. The white-emission of the two-wavelength type was successfully obtained by using both nitro-DPVT for blue~emitting material, orange emission as a host material and Rubrene for orange emission as a guest material. The basic structure of the fabricated white OLED is glass/ITO/NPB$(200{\AA})$/nitro-DPVT$(100{\AA})$/nitro-DPVT:$Rubrene(100{\AA})/BCP(70{\AA})/Alq_3(150{\AA})/Al(600{\AA})$. To evaluate the. characteristics of the devices, firstly, we varied the doping concentrations of fluorescent dye Rubrene from 0.5 % to 0.8 % to 1.3 % to 1.5 % to 3.0 % by weight. A nearly pure white-emission was obtained in CIE coordinates of (0.3259, 0.3395) when the doping concentration of Rubrene was 1.3 % at an applied voltage of 18 V. Secondly, we varied the thickness of the NPB layer from $150{\AA}\;to\;200{\AA}\;to\;250{\AA}\;to\;300{\AA}$ by fixing doping with of Rubrene at 1.3 %. A nearly pure white-emission was also obtained in CIE coordinates of (0.3304, 0.3473) when the NPB layer was $250-{\AA}$ thick at an applied voltage of 16 V. The two devices started to operate at 4 V and to emit light at 4.5 V. The external quantum efficiency was above 0.4 % when almost all of the current was injected.

Anticancer activity and potential mechanisms of 1C, a ginseng saponin derivative, on prostate cancer cells

  • Wang, Xu De;Su, Guang Yue;Zhao, Chen;Qu, Fan Zhi;Wang, Peng;Zhao, Yu Qing
    • Journal of Ginseng Research
    • /
    • v.42 no.2
    • /
    • pp.133-143
    • /
    • 2018
  • Background: AD-2 (20(R)-dammarane-3b, 12b, 20, 25-tetrol; 25-OH-PPD) is a ginsenoside and isolated from Panax ginseng, showing anticancer activity against extensive human cancer cell lines. In this study, effects and mechanisms of 1C ((20R)-3b-O-(L-alanyl)-dammarane-12b, 20, 25-triol), a modified version of AD-2, were evaluated for its development as a novel anticancer drug. Methods: MTT assay was performed to evaluate cell cytotoxic activity. Cell cycle and levels of reactive oxygen species (ROS) were determined using flow cytometry analysis. Western blotting was employed to analyze signaling pathways. Results: 1C concentration-dependently reduces prostate cancer cell viability without affecting normal human gastric epithelial cell line-1 viability. In LNCaP prostate cancer cells, 1C triggered apoptosis via Bcl-2 family-mediated mitochondria pathway, downregulated expression of mouse double minute 2, upregulated expression of p53 and stimulated ROS production. ROS scavenger, N-acetylcysteine, can attenuate 1C-induced apoptosis. 1C also inhibited the proliferation of LNCaP cells through inhibition on $Wnt/{\beta}-catenin$ signaling pathway. Conclusion: 1C shows obvious anticancer activity based on inducing cell apoptosis by Bcl-2 family-mediated mitochondria pathway and ROS production, inhibiting $Wnt/{\beta}-catenin$ signaling pathway. These findings demonstrate that 1C may provide leads as a potential agent for cancer therapy.

Study on the photo-induced refractive index change of diarylethene derivative using fiber-to-planar waveguide coupler (광섬유-평면도파로 결합기를 이용한 광변색성 디아릴에텐 유도체의 광유도 굴절률 변화에 관한 연구)

  • 조강민;윤정현;임선정;박수영;강신원
    • Korean Journal of Optics and Photonics
    • /
    • v.15 no.2
    • /
    • pp.109-113
    • /
    • 2004
  • We have manufactured and characterized the fiber-to-Planar Waveguide Coupler for analysis of the photo-induced refractive index change of DM-BTE .(l,2-bis[2,5-dimethylthio-phen-3-yl]-hexafluorocyclopentene) When irradiated with ultraviolet light, the colorless diarylethene(DM-BTE)-crystal turned red while keeping the crystal shape. The red color was bleached by irradiation with visible light(λ>450 nm). The resonant wavelength was shifted and recovered owing to the refractive index variation of the planar waveguide because of its photo-functional properties on exposure to UV and visible light. The wavelength responses of this switch by UV exposure were measured as 0.057 nm/sec with saturation time of 60 seconds. and when illuminated by visible light, resonance wavelength variations were measured as 0.028 nm/sec, with recovery time of 140 seconds.

Analysis of Subthreshold Swing for Double Gate MOSFET Using Gaussian Function (가우스함수를 이용한 DGMOSFET의 문턱전압이하 스윙분석)

  • Jung, Hak-Kee;Han, Ji-Hyung;Lee, Jae-Hyung;Jeong, Dong-Soo;Lee, Jong-In;Kwon, Oh-Shin
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
    • /
    • 2011.05a
    • /
    • pp.681-684
    • /
    • 2011
  • In this paper, the relationship of potential and charge distribution in channel for double gate(DG) MOSFET has been derived from Poisson's equation using Gaussian function. The subthreshold swing has been investigated according to projected range and standard projected deviation, variables of Gaussian function. The analytical potential distribution model has been derived from Poisson's equation, and subthreshold swing has been obtained from this model. The subthreshold swing has been defined as the derivative of gate voltage to drain current and is theoretically minimum of 60mS/dec, and very important factor in digital application. Those results of this potential model are compared with those of numerical simulation to verify this model. As a result, since potential model presented in this paper is good agreement with numerical model, the subthreshold swings have been analyzed according to the shape of Gaussian function.

  • PDF

Estimation of Hydrodynamic Derivatives of Submarine Model by Using VPMM Test (VPMM 시험을 이용한 잠수함 모형의 유체력 미계수 추정)

  • Jung, Jin-Woo;Jeong, Jae-Hun;Kim, In-Gyu;Lee, Seung-Keon
    • Journal of Navigation and Port Research
    • /
    • v.38 no.2
    • /
    • pp.97-103
    • /
    • 2014
  • In these days, the world has been increasing navy forces such as aircraft carriers and high-tech destroyers etc. and the importance of submarines is being emphasized. Therefore, accurate values of the derivatives in equations of motion are required to control motion of the submarines. Hydrodynamic derivatives were measured by the vertical planar motion mechanism(VPMM) model test. VPMM equipment gave pure heave and pitch motion respectively to the submarine model and the forces and moments were acquired by load cells. As a result, the hydrodynamic derivatives of the submarine are provided through the Fourier analysis of the forces and moments in this paper.