Analytical Science and Technology (분석과학)
- Volume 9 Issue 2
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- Pages.198-202
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- 1996
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- 1225-0163(pISSN)
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- 2288-8985(eISSN)
Determination of Histamine by Reverse-Phase High Performance Liquid Chromatography with Fluorescence Detection
형광검출 역상 액체크로마토그래피에 의한 Histamine의 정량
- Ryu, Hee-Chun (College of Pharmacy, Woosuk University) ;
- Kim, Hyung-Ryong (Wonkwang University School of Dentistry) ;
- Kim, Sang-Hyun (College of Pharmacy, Woosuk University) ;
- Kim, Dae-Ki (Wonkwang University School of Medicine) ;
- Lee, Young-Mi (Wonkwang University School of Medicine) ;
- Kim, Hyung-Min (Wonkwang University School of Medicine) ;
- An, Nyeon-Hyoung (College of Pharmacy, Wonkwang University) ;
- Shin, Tae-Yong (College of Pharmacy, Woosuk University)
- 유희춘 (우석대학교 약학대학) ;
- 김형룡 (원광대학교 치과대학) ;
- 김상현 (우석대학교 약학대학) ;
- 김대기 (원광대학교 의과대학) ;
- 이영미 (원광대학교 의과대학) ;
- 김형민 (원광대학교 의과대학) ;
- 안년형 (원광대학교 약학대학) ;
- 신태용 (우석대학교 약학대학)
- Received : 1996.02.13
- Published : 1996.06.25
Abstract
A rapid and simple method for the determination of histamine by reverse-phase high performance liquid chromatography with fluorescence detection was established. 9-Fluorenylmethyl chloroformate(FMOC) was used as fluorescent derivative reagent. The optimum conditions for the derivatization such as pH, reaction time and the concentration of FMOC were investigated. Linearity of calibration curve was obtained between
Histamine을 정확하고 신속하게 정량하기 위해 9-Fluorenylmethyl chloroformate를 형광유도체화제로 하여 역상 HPLC법으로 정량하였다. 히스타민을 형광유도체화할 때 반응액의 pH, 반응시간, 형광유도체화제의 농도 등 최적 반응 조건을 검토하였다. 이 방법으로 히스타민을 분석한 결과