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Development of cordycepin fortified meat production in Hanwoo steers II. Effects of mycelia of Cordyceps militaris cultured from grains on cordycepin deposition in muscles of finishing Hanwoo steers (Cordycepin 강화 한우고기 생산에 관한 연구 II. 곡립기주 동충하초 균사체 급여가 한우고기내 cordycepin 축적에 미치는 영향)

  • Kim, W.Y.;Lee, S.H.;Kim, D.H.;Lee, J.H.;Nho, W.G.;Hwang, J.H.;Yeo, J.M.
    • Journal of Practical Agriculture & Fisheries Research
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    • v.11 no.1
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    • pp.53-61
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    • 2009
  • The present experiment was conducted to examine the effects of mycelia of Cordyceps militaris cultured from grains on cordycepin deposition in muscles and plasma glutathione peroxidase activity in finishing Hanwoo steers. Four steers were divided into two groups. Control group received no supplement whereas treatment group received 1 % mycelia of C. militaris of their feed intake for 80 days prior to slaughtering. Final body weight, average daily gain, dry matter intake were not affected by the treatment. But the activity of plasma glutathione peroxidase was numerically higher for the treatment group than for the control group (15.70 vs 9.23 unit). Furthermore, cordycepin deposition in muscles on the treatment group was identified with thin layer chromatography. The results of the present experiment show that cordycepin could be deposited in muscles of Hanwoo beef by feeding mycelia of Cordyceps militaris cultured from grains.

First Report of Tomato Spotted Wilt Virus in Oxypetalum coeruleum in Korea (옥시페탈룸에서 발생한 토마토반점위조바이러스 국내 첫 보고)

  • Eseul, Baek;Peter, Palukaitis;Ju-Yeon, Yoon
    • Research in Plant Disease
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    • v.28 no.4
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    • pp.231-236
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    • 2022
  • Oxypetalum coeruleum, commonly known as Tweedia, is a perennial herbaceous plant of the Apocynaceae family native to southern Brazil and Uruguay. Tweedia plants are grown as one of the most popular ornamental flowers for floral arrangement in Korea. In May 2021, several tweedia plants in a single greenhouse in Gimje, Jeollabuk-do were found to show virus-like symptoms including necrotic rings, vein-clearing, chlorotic mottle, and mosaic on the leaves, and necrosis on the stems. Here, we have identified tomato spotted wilt virus (TSWV) in symptomatic tweedia leaves by applying high-throughput RNA sequencing. In the result, a single infection by TSWV was verified without mixed infections of different virus species. To confirm the presence of TSWV, a reverse transcription polymerase chain reaction was performed with a specific primer set to the N gene of TSWV. The complete genomic sequence of L, M, and S segments of TSWV 'Oxy' isolate were determined and deposited in GenBank under accession numbers LC671525, LC671638, and LC671639, respectively. In the phylogenetic tree analysis by maximum likelihood method, 'Oxy' isolate showed a high relationship with TSWV 'Gumi' isolate from Gerbera jamesonii in Gyeongsangbuk-do, Korea; for all three RNA segments. To our knowledge, this is the first report of TSWV infection of O. coeruleum in Korea.

Genetic Environments at the Ssangjeon Tungsten-bearing Hydrothermal Vein Deposit (쌍전 함 텅스텐 열수 맥상광상의 생성환경)

  • Sunjin Lee;Sang-Hoon Choi
    • Economic and Environmental Geology
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    • v.55 no.6
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    • pp.689-699
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    • 2022
  • The Ssangjeon tungsten deposit is located within the Yeongnam Massif. Within the area a number of hydrothermal quartz veins were formed by narrow open-space filling of parallel and subparallel fractures in the metasedimentary rocks as Wonnam formation, Buncheon granite gneiss, amphibolite and/or pegmatite. Mineral paragenesis can be divided into two stages (stage I, ore-bearing quartz vein; stage II, barren quartz vein) by major tectonic fracturing. Stage I, at which the precipitation of major ore minerals occurred, is further divided into three substages (early, middle and late) with paragenetic time based on minor fractures and discernible mineral assemblages: early, marked by deposition of arsenopyrite with pyrite; middle, characterized by introduction of wolframite and scheelite with Ti-Fe-bearing oxides and base-metal sulfides; late, marked by Bi-sulfides. Fluid inclusion data show that stage I ore mineralization was deposited between initial high temperatures (≥370℃) and later lower temperatures (≈170℃) from H2O-CO2-NaCl fluids with salinities between 18.5 to 0.2 equiv. wt. % NaCl of Ssangjeon hydrothermal system. The relationship between salinity and homogenization temperature indicates a complex history of boiling, fluid unmixing (CO2 effervescence), cooling and dilution via influx of cooler, more dilute meteoric waters over the temperature range ≥370℃ to ≈170℃. Changes in stage I vein mineralogy reflect decreasing temperature and fugacity of sulfur by evolution of the Ssangjeon hydrothermal system with increasing paragenetic time.

Estimation of Weight Distribution of Rockfall Block by Joint Measurement And Study on Its Application to Rockfall Simulation (절리조사결과에 의한 현장 낙석무게분포추정 및 추정결과의 낙석시뮬레이션 적용성 검토)

  • Kim, Dong-Hee;Ryu, Dong-Woo;Kim, Su-Chul;Yoon, Sang-Kil;Lee, Woo-Jin
    • Journal of the Korean Geotechnical Society
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    • v.23 no.11
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    • pp.67-76
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    • 2007
  • The characteristics of rockfall are determined by virtually all factors and conditions e.g. the physical figure of the slope such as inclination, height, roughness, the elemental figure of the slope such as vegetation and material deposited, and the shape and weight of the rockfall itself. Although it is one of the major factors to be considered in rockfall simulation, little attention has been given to the weight of the rockfall. And, since the size of the rockfall is dominated by joint spacing, the distribution of the rockfall block weight can be predicted as a function of the joint spacing. In this study, the weight distribution of rockfall was estimated by using the method of volumetric joint count, $J_{\nu}$, based on joint spacing, and $RQD-J_{\nu}$. The results indicate that the weight distributions were analogous in two methods, and the distribution was to be $75.3{\sim}76.7%$ for 200 kilograms or lesser, $15.0{\sim}16.6%$ for $200{\sim}400$ kilograms, and $6.7{\sim}9.7%$ for 400 kilograms or more, which show good matches with the actual on-site weight distribution. Therefore, the weight distribution of rockfall suggested in this paper is able to be considered as appropriate data for rockfall simulation.

Effect of Deposition and Heat Treatment Conditions on the Electrical and Optical Properties of AZO/Cu/AZO Thin Film (증착 및 열처리 조건에 따른 AZO/Cu/AZO 박막의 전기적·광학적 특성 평가)

  • Chan-Young Kim;Ha-Eun Lim;Gaeun Yang;Sukjeang Kwon;Chan-Hee Kang;Sang-Chul Lim;Taek Yeong Lee
    • Korean Journal of Materials Research
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    • v.33 no.4
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    • pp.142-150
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    • 2023
  • AZO/Cu/AZO thin films were deposited on glass by RF magnetron sputtering. The specimens showed the preferred orientation of (0002) AZO and (111) Cu. The Cu crystal sizes increased from about 3.7 nm to about 8.5 nm with increasing Cu thickness, and from about 6.3 nm to about 9.5 nm with increasing heat treatment temperatures. The sizes of AZO crystals were almost independent of the Cu thickness, and increased slightly with heat treatment temperature. The residual stress of AZO after heat treatment also increased compressively from -4.6 GPa to -5.6 GPa with increasing heat treatment temperature. The increase in crystal size resulted from grain growth, and the increase in stress resulted from the decrease in defects that accompanied grain growth, and the thermal stress during cooling from heat treatment temperature to room temperature. From the PL spectra, the decrease in defects during heat treatment resulted in the increased intensity. The electrical resistivities of the 4 nm Cu film were 5.9×10-4 Ω·cm and about 1.0×10-4 Ω·cm for thicker Cu films. The resistivity decreased as the temperature of heat treatment increased. As the Cu thickness increased, an increase in carrier concentration resulted, as the fraction of AZO/Cu/AZO metal film increased. And the increase in carrier concentration with increasing heat treatment temperature might result from the diffusion of Cu ions into AZO. Transmittance decreased with increasing Cu thicknesses, and reached a maximum near the 500 nm wavelength after being heat treated at 200 ℃.

A Study on the High Content Ceramide Stabilization Formulation with Cyclodextrin (사이클로덱스트린을 함유한 고함량 세라마이드 안정화 제형 연구)

  • Ye Ji Kim;Sang Woo Han;So Min Lee;Byungsun Cha;Hyojin Heo;Sofia Brito;Lei Lei;Sang Hun Lee;You-Yeon Chun;Ha Hyeon Jo;Hyung Mook Kim;Byeong-Mun Kwak;Bum-Ho Bin
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.49 no.2
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    • pp.97-106
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    • 2023
  • In this study, we would like to study the stabilization of the high content of ceramide formulation by containing cyclodextrin. Ceramide, which constitutes the intercellular lipid, a human skin barrier, is a very important ingredient in moisturizing maintenance by protecting moisture in the skin and strengthening the skin barrier. However, since ceramide is poorly soluble, even if it is included in the cosmetic formulation, it has a problem that it is slowly gelled or crystallized and deposited over time, making it difficult to containing a high amount of ceramide. Cyclodextrin is a cyclic oligosaccharide connected with glucose molecules and has a cylindrical structure with hydrophilic outer surface and hydrophobic inner surface, which is known to improve the physicochemical properties of drugs such as improving solubility and absorption of poorly soluble drugs. We demonstrated the stability of the formulation containing high amount of ceramide by measuring hardness and observing emulsion drops with polarized microscope. This study also demonstrated that the high-content ceramide formulation containing cyclodextrin has the effect of preventing gelation or crystallization of ceramide, thus having excellent environmental conditions stability and skin moisturization.

Quantitative analysis of hydrogen in thin film by scattering-recoil co-measurement technique (산란-되튐 동시 측정 방법에 의한 박막 중 수소 정량법)

  • Lee, Hwa-Ryun;Eum, Chul Hun;Choi, Han-Woo;Kim, Joonkon
    • Analytical Science and Technology
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    • v.19 no.5
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    • pp.400-406
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    • 2006
  • Hydrogen analysis by elastic recoil detection has been performed utilizing polyimide film as a reference sample of known hydrogen content assuming the soundness of ion beam current integration. However beam current integration at higher incidence angle is not reliable. Scattering yield per unit fluence by current integration which is normalized per unit path length decreases as the sample tilt angle is getting higher. Moreover because beam current integration at high tilt angle is incomplete, hydrogen evaluation is very risky by direct comparison of sequentially collected recoil spectra between reference and target sample. In this study, primary ion beam dose is determined by backscattering spectrum that is collected simultaneously with recoil spectrum instead of ion beam current integration in order to reduce uncertainty arising in the process of current integration and to enhance the reliability of quantitative analysis. Three test samples are selected $-7.6{\mu}m$ polyimide film, hydrogen implanted silicondioxide and Au deposited carbon wafer- and analyzed by two methods and compared.

Study on fatty acids composition by latent fingerprint deposition (유류된 잠재지문의 지방산조성에 관한 연구)

  • Choi, Mi Jung;Ha, Jaeho;Park, Sung Woo
    • Analytical Science and Technology
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    • v.21 no.3
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    • pp.212-221
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    • 2008
  • In order to investigate the information for effective detection and developing of latent fingerprints, we identified fatty acids composition of latent fingerprints on non-porous evidence surface and the chemical changes of latent fingerprint residue after print deposition during 7 months. Fingerprints from eight Korean male donors (aged 29-50 years) and one female donor (aged 36 years) were collected. All fingerprints were found to contain lauric acid (C12:0), myristic acid (C14:0), palmitic acid (C16:0), stearic acid (C18:0), elaidic acid (C18:1n9t), oleic acid (C18:1n9c), linoleic acid (C18:2n6c), arachidic acid (C20:0), linolenic acid (C18:3n3), erucic acid (C22:1n9) and docosadienoic acid (C22:2) and primarily palmitic acid (35.45-48.37%), oleic acid (14.84-28.49%), stearic acid (9.71-24.96%) and linoleic acid (7.68-18.8%) occupied 75% of total fatty acids. When the fingerprints were deposited at dark room for 7 months, total fatty acids components decreased about 12-25%. It can be explained that significant degradation of long-chain fatty acids such as elaidic acid (C18:1n9t), arachidic acid (C20:0), linolenic acid (C18:3n3), erucic acid (C22:1n9), and docosadienoic acid (C22:2) resulted in the generation of myristic acid (C14:0), myristoleic acid (C14:1) and pentadecanoic acid (C15:0).

Developing of latent fingerprint on human skin (생체피부에서의 잠재지문 현출)

  • Lee, Hee-Il;Choi, Mi-Jung;Kim, Jai-Hoon;Park, Sung-Woo
    • Analytical Science and Technology
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    • v.21 no.3
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    • pp.222-228
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    • 2008
  • On living skin the chances of a successfully developing latent fingerprint are very limited. This is due to the fact that continual perspiration and rapid absorption diffuse into the lipophillic layer on skin. A study was conducted to investigate effectively developing method of latent fingerprints on human skin surfaces and pig skin likely corpse's skin. We used commercial fingerprint powder, black powders, black magnetic powder, fluorescence magnetic powder, Cyanoacrylate fuming (CA) and direct lifting methods (lifting paper, glasses and photo glossy paper). Developing of fresh fingerprints on living skin was achieved with S-powderblack, CA fuming and CA fuming following S-powder, fluorescence powder. The other powder tends to overwhelm the latent print and the background. But, latent fingerprint residue was disappeared with time after deposit on a living surface. In case of pig skin likely corpse's skin, latent fingerprint detection was achieved with CA fuming following S-powder and deposited print during 6 hr at $25^{\circ}C$, 40% relative moisture yielded excellent fingerprints with clear ridge details using 1 min CA fuming. And enhancement of fingerprint detection image using forensic light source was achieved.

Synthesis of La0.7Sr0.3Mn1-xIrxO3 thin-films in search of superconductivity

  • Byeongjun Seok;Youngdo Kim;Donghan Kim;Jongho Park;Changyoung Kim
    • Progress in Superconductivity and Cryogenics
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    • v.25 no.2
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    • pp.10-13
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    • 2023
  • High-TC superconductivity (HTSC) has been the central issue in the field of condensed matter physics for decades. An essential part of the research on superconductivity is finding new exotic superconductors. It was recently suggested that Ir-substituted La0.7Sr0.3MnO3 (LSMIO) is a new high-TC superconductor. However, systematic studies to experimentally verify the superconductivity have not been done. Here, we report the growth processes of LSMIO thin films and their electrical transport properties. We observed a clear negative correlation between the intensity of the laser utilized for film deposition and the Curie temperature of the deposited film. We attributed this effect to the suppression of Sr concentration in the LSMIO films as the laser intensity increased. However, our LSMIO films show conventional ferromagnetism instead of HTSC. To realize the HTSC in LSMIO systems, further exploration of diverse compositions of LSMIO compounds is essential.