Browse > Article
http://dx.doi.org/10.5423/RPD.2022.28.4.231

First Report of Tomato Spotted Wilt Virus in Oxypetalum coeruleum in Korea  

Eseul, Baek (Department of Horticultural Sciences, Seoul Women's University)
Peter, Palukaitis (Department of Horticultural Sciences, Seoul Women's University)
Ju-Yeon, Yoon (Department of Plant Protection and Quarantine, Jeonbuk National University)
Publication Information
Research in Plant Disease / v.28, no.4, 2022 , pp. 231-236 More about this Journal
Abstract
Oxypetalum coeruleum, commonly known as Tweedia, is a perennial herbaceous plant of the Apocynaceae family native to southern Brazil and Uruguay. Tweedia plants are grown as one of the most popular ornamental flowers for floral arrangement in Korea. In May 2021, several tweedia plants in a single greenhouse in Gimje, Jeollabuk-do were found to show virus-like symptoms including necrotic rings, vein-clearing, chlorotic mottle, and mosaic on the leaves, and necrosis on the stems. Here, we have identified tomato spotted wilt virus (TSWV) in symptomatic tweedia leaves by applying high-throughput RNA sequencing. In the result, a single infection by TSWV was verified without mixed infections of different virus species. To confirm the presence of TSWV, a reverse transcription polymerase chain reaction was performed with a specific primer set to the N gene of TSWV. The complete genomic sequence of L, M, and S segments of TSWV 'Oxy' isolate were determined and deposited in GenBank under accession numbers LC671525, LC671638, and LC671639, respectively. In the phylogenetic tree analysis by maximum likelihood method, 'Oxy' isolate showed a high relationship with TSWV 'Gumi' isolate from Gerbera jamesonii in Gyeongsangbuk-do, Korea; for all three RNA segments. To our knowledge, this is the first report of TSWV infection of O. coeruleum in Korea.
Keywords
Identification; Oxypetalum coeruleum; Tomato spotted wilt virus;
Citations & Related Records
Times Cited By KSCI : 6  (Citation Analysis)
연도 인용수 순위
1 Cho, S.-Y., Kim, S.-M., Kim, S. and Lee, B. C. 2020. First report of tomato spotted wilt virus infecting Arachis hypogaea in Korea. J. Plant Pathol. 102: 271. 
2 Choi, H. S., Lee, S. H., Kim, M. K., Kwak, H. R., Kim, J. S., Cho, J. D. et al. 2010. Occurrence of virus diseases on major crops in 2009. Res. Plant Dis. 16: 1-9. (In Korean)    DOI
3 Choi, S.-K., Cho, I. S., Choi, G.-S. and Yoon, J.-Y. 2014. First report of tomato spotted wilt virus in Brugmansia suaveolens in Korea. Plant Dis. 98: 1283. 
4 Chung, B. N., Pak, H. S., Jung, J. A. and Kim, J. S. 2006. Occurrence of tomato spotted wilt virus in chrysanthemum (Dendranthema grandiflorum) in Korea. Plant Pathol. J. 22: 230-234.    DOI
5 Chung, B. N., Yoon, J.-Y. and Cho, I.-S. 2021. First report of tomato spotted wilt virus on Iris domestica in South Korea. Res. Plant Dis. 27: 32-37.    DOI
6 Dominguez, G. R., Siclan, M. L. S., Ortigoza, C. A., Martinez, J. G. A. and Davila, J. F. R. 2019. Tomato spotted wilt Orthotospovirus (TSWV) is not transmitted by tomato seed. Rev. Mex. Cienc. Agric. 10: 1449-1455. 
7 Elliott, M. S., Massey, B., Cui, X., Hiebert, E., Charudatan, R., Waipara, N. et al. 2009. Supplemental host range of Araujia mosaic virus, a potential biological control agent of moth plant in New Zealand. Aust. Plant Pathol. 38: 603-607.    DOI
8 EPPO. 2020. EPPO datasheets on pests recommended for regulation. Tomato spotted wilt virus. URL http://www.eppo.org [11 August 2022]. 
9 Kim, J.-H., Choi, G.-S., Kim, J.-S. and Choi, J.-K. 2004. Characterization of tomato spotted wilt virus from paprika in Korea. Plant Pathol. J. 20: 297-301    DOI
10 Kim, M., Kim, J. E., Kim, J., Kwak, H. R., Choi, H. S., Lee, H. J. et al. 2018. First report of tomato spotted wilt virus in Hoya carnosa in Korea. Plant Dis. 102: 1672. 
11 Kumar, S., Stecher, G., Li, M., Knyaz, C. and Tamura, K. 2018. MEGA X: molecular evolutionary genetics analysis across computing platforms. Mol. Biol. Evol. 35: 1547-1549.    DOI
12 Kwak, H.-R., Hong, S.-B., Choi, H.-Y., Park, G., Hur, O.-S., Byun, H.-S. et al. 2021. First report of tomato spotted wilt virus in Angelica acutiloba. Res. Plant Dis. 27: 84-90.    DOI
13 Kwak, H.-R., Son, S.-W., Choi, H.-Y., Go, W.-R., Kim, J.-E. Baek, E. et al. 2020. First report of tomato spotted wilt virus in Lycium chinense. Aust. Plant Dis. Notes 15: 5. 
14 Morse, J. G. and Hoddle, M. S. 2006. Invasion biology of thrips. Annu. Rev. Entomol. 51: 67-89.    DOI
15 Ok, J. J. 2009. A study on the recent trend and characteristics of the bridal bouquet. J. Korea Floral Art Res. Assoc. 13: 25-36. 
16 Ortiz, T. A., Moritz, A., de Oliveira, M. A., Lone, A. B., Nakatani, S. H. and Takahashi, L. S. A. 2014. Optimal conditions for germination of seeds of Epiphyllum oxypetalum. Afr. J. Agric. Res. 9: 2630-2637.    DOI
17 Parrella, G., Gognalons, P., Gebre-Selassie, K., Vovlas, C. and Marchoux, G. 2003. An update of the host range of tomato spotted wilt virus. J. Plant Pathol. 85: 227-264. 
18 Verhoeven, J. T. J. and Roenhort, J. W. 1998. Occurrence of tospovirus in the Netherland. In: Recent Progress in Tospovirus and Thrips Research, eds. By D. Peters and R. Golbach, pp. 77-80. Wageningen Agricultural University, Section Virology, Wageningen, The Netherlands.
19 Yoon, J. Y., Choi, G. S. and Choi, S. K. 2016. First report of tomato spotted wilt virus in Eustoma grandiflorum in Korea. Plant Dis. 101: 515 
20 Yoo, E. H., Kim, K. J., Jeong, S. J., Han, S. W., Jeong, M. I., Lee, D. W. et al. 2010. Characteristics of floral materials used for commercial flower baskets in Korean Online Market. Korean J. Hortic. Sci. Technol. 28: 156-157. 
21 Yoon, J. Y., Choi, G. S., Kwon, S. J. and Choi, I. S. 2019. First report of tomato spotted wilt virus infecting Peperomia obtusifolia in South Korea. Plant Dis. 103: 593.