• 미생물과산업
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• 제12권1호
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• pp.28-34
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• 1986

이글은 Esser, K. & Oeser, B.의 논문으로 해외근착자료로 소개한다. Nucleic acids do not only carry the genetic information, but are also the only substances being able of self-replication. Molecular cloning, an essential tool in biotechnology, requires among other things, an understanding of the mechanisms of replication which at present is fairly well known. After an introduction to the general principle, the status of art on replication procedure and its implication for biotechnology are dealt with.

• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 2001년도 ICCAS
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• pp.49.3-49
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• 2001

In 1994 Adelman´s pioneer work demonstrated that deoxyribonucleic acid (DNA) could be used as a medium for computation to solve mathematical problems. He described the use of DNA based computational approach to solve the Hamiltonian Path Problem (HPP). Since then a number of combinatorial problems have been analyzed by DNA computation approaches including, for example: Maximum Independent Set (MIS), Maximal Clique and Satisfaction (SAT) Problems. In the present paper we propose a method of solving another classic combinatorial optimization problem - the eraph Coloring Problem (GCP), using specifically designed circular DNA plasmids as a computation tool. The task of the analysis is to color the graph so that no two nodes ...

• Journal of Yeungnam Medical Science
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• 제37권2호
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• pp.73-78
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• 2020

Cancer incidence has been increasing steadily and is the leading cause of mortality worldwide. Gastric cancer is still most common malignancy in Korea. Cancer initiation and progression are multistep processes involving various growth factors and their ligands. Among these growth factors, we have studied hepatocyte growth factor (HGF), which is associated with cell proliferation and invasion, leading to cancer and metastasis, especially in gastric cancer. We explored the intercellular communication between HGF and other surface membrane receptors in gastric cancer cell lines. Using complimentary deoxyribonucleic acid microarray technology, we found new genes associated with HGF in the stomach cancer cell lines, NUGC-3 and MKN-28, and identified their function within the HGF pathway. The HGF/N-methyl-N'-nitroso-guanidine human osteosarcoma transforming gene (c-MET) axis interacts with several molecules including E-cadherin, urokinase plasminogen activator, KiSS-1, Jun B, and lipocalin-2. This pathway may affect cell invasion and metastasis or cell apoptosis and is therefore associated with tumorigenesis and metastasis in gastric cancer.

• BMB Reports
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• 제30권1호
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• pp.37-40
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• 1997

Earthworm extracts are known for anti-inflammatory, analgesic. antipyretic, and anticancer effects but can also influence blood circulation. It was previously shown that an earthworm, Lumbricus rubelius. contained a water-extractable anticoagulant which was a heat- and acid-stable molecule with hydrophilic property. In order to uncover the biochemical nature of this molecule, the anticoagulant was processed with various hydrolases such as trypsin, DNase, RNase. and lysozome. When the digested samples were analyzed with an in vitro coagulation test measuring activated partial thromboplastin time (APTT) and agarose gel electrophoresis, the anticoagulant proved to be a relatively homogeneous DNA fragment with relative molecular size around 72 base pairs. Interestingly, the activity was further stimulated with a trypsin digestion. RNA. on the other hand, did not prolong the APTT. It was also demonstrated that the DNA accelerated the antithrombin III (AT-III) inhibition of thrombin from $IC_{50}$ of 0.34 to 0.16 unit determined with S-2238 as a substrate, whereas heparin, a popular anticoagulant. shifted the value to 0.05. Therefore, it is suggested that the DNA could be considered as an alternative antithrombotic agent to heparin, which would exhibits bleeding side effects.

• Plant Biotechnology Reports
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• 제2권4호
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• pp.227-231
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• 2008

D-class cyclins play important roles in controlling the cell cycle in development and in response to external signals by forming the regulatory subunit of cyclin-dependent kinase (CDK) complexes. To evaluate the effects of D-class cyclins in transgenic rice plants, Arabidopsis cyclin D2 gene (CycD2) was linked to the maize ubiquitin1 promoter (Ubi1) and introduced into rice by the Agrobacterium-mediated transformation method. Genomic deoxyribonucleic acid (DNA), ribonucleic acid (RNA), and Western blot hybridizations of the Ubi1:-CycD2 plants revealed copy number of transgene and its increased expression in leaf and callus cells at messenger RNA (mRNA) and/or protein levels. The H1 kinase assay using the immunoprecipitates of protein extracts from the Ubi1:CycD2 plants and nontransgenic controls demonstrated that the introduced Arabidopsis CycD2 forms a functional CycD2/CDK complex with an unidentified CDK of rice. Shoot and root growth was enhanced in the Ubi1:CycD2 seedlings compared with nontransgenic controls, together, suggesting that Arabidopsis cyclin D2 interacts with a rice cyclin-dependent kinase, consequently enhancing seedling growth.

• Journal of Microbiology
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• 제39권2호
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• pp.133-138
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• 2001

We investigated the antagonism of indigenous bacteria isolated from stressed mussels and their extracellular metabolites on the adult zebra mussel, Dreissena polymorpha. Selective bacterial isolates including Aeromonas media, A. salmonicida, A. veronii, and Shewanella putrefaciens, showed strong lethality against adult mussels and 100% mortality was observed within 5 days of incubation. Bacterial metabolites, fractionated and concentrated from stationary-phase culture supernatants of these bacterial isolates, displayed varying degrees of antagonistic effects on zebra mussels. Among the three size fractions examined, <5, 5-10, and >10 kDa, the mast lethal fraction seems to be >10 kDa for three of the four isolates tested. Further chemical analyses of these size fractions revealed that the predominant constituents were polysaccharides and proteins. No 2-keto-3-deoxyoctanoic acid (2-KDO), deoxyribonucleic acids (DNA) or uranic acid were detectable. Extraction of supernatants of two antagonistic isolates with polar solvent suggested that polar molecules are present in the active fraction. Our data suggest that extracellular metabolites produced by antagonistic bacteria are also involved in disease development in zebra mussels and elucidation of the mechanisms involved may offer a novel strategy for control of biofouling invertebrates.

• Natural Product Sciences
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• 제10권4호
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• pp.190-195
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• 2004

The anticancer potency of the ethanolic extract of Terminalia arjuna on N-nitrosodiethylamine (DEN) induced hepatocellular carcinoma in Wistar albino rats was studied. Single intraperitoneal injection of DEN was administrated to induce liver cancer. After two weeks, phenobarbital (PB) was given orally for fourteen weeks to promote the cancer. The cancer bearing animals treated with ethanolic extract of T.arjuna (400 mg/kg body weight) for 28 days. Nucleic acids such as deoxyribonucleic acid (DNA) and ribonucleic acid (RNA) were estimated in liver and kidney of control and experimental animals. Certain marker enzymes viz, alanine aminotransaminase (ALT), aspartate aminotransaminase (AST), acid phosphatase (ACP), alkaline phosphatase (ALP), 5'-nucleotidase (5'ND) and lactate dehydrogenase (LDH) were assayed in serum, liver and kidney of control and experimental animals. The levels of DNA and RNA were significantly increased in cancer bearing animals. The activities of ALT, AST, ACP, ALP, 5'ND, and LDH were significantly (P<0.001) increased in serum of cancer bearing animals. On the other hand, the levels of ALT, AST were decreased (P<0.001) and ACP, ALP, 5'ND, and LDH were significantly increased (P<0.001) in liver and kidney. These changes were reversed to near normal in drug treated animals. These observations suggest that the ethanolic extract of T.arjuna possess anticancer activity.

• 한국지능시스템학회논문지
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• 제17권2호
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• pp.167-172
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• 2007

많은 유전자 정보와 그 부산물은 많은 방법을 통해 연구되어 왔다. DNA 마이크로어레이 기술의 사용은 많은 데이터를 가져왔으며, 이렇게 얻은 데이터는 기존의 연구 방법으로는 분석하기 힘들다. 본 논문에서는 많은 양의 데이터를 처리할 수 있게 하기 위하여 K-means 클러스터링 알고리즘을 이용한 분할 클러스터링을 제안하였다. 제안한 방법을 쌀 유전자로부터 나온 마이크로어레이 데이터에 적용함으로써 제안된 클러스터링 방법의 유용성을 검증하였으며, 기존의 K-means 클러스터링 알고리즘을 적용한 결과와 비교함으로써 제안된 알고리즘의 우수성을 확인할 수 있었다.

• Smart Structures and Systems
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• 제12권1호
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• pp.73-95
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• 2013

Nine deoxyribonucleic acid (DNA) sequences were used to functionalize single-walled carbon nanotube (SWNT) sensors to detect the trace amount of methanol, acetone, and HCl in vapor. DNA 24 Ma (24 randomly arranged nitrogenous bases with one amine at each end of it) decorated SWNT sensor and DNA 24 A (only adenine (A) base with a length of 24) decorated SWNT sensor have demonstrated the largest sensing responses towards acetone and HCl, respectively. On the other hand, for the DNA GT decorated SWNT sensors with different sequence lengths, the optimum DNA sequence length for acetone and HCl sensing is 32 and 8, separately. The detection of methanol, acetone, and HCl have identified that DNA functionalized SWNT sensors exhibit great selectivity, sensitivity, and repeatability with an accuracy of more than 90%. Further, a sensor array composed of SWNT functionalized with various DNA sequences was utilized to identify acetone and HCl through pattern recognition. The sensor array is a combination of four different DNA functionalized SWNT sensors and two bare SWNT sensors (work as reference). This wireless sensing system has enabled real-time gas monitoring and air quality assurance for safety and security.

• Architectural research
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• 제12권2호
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• pp.1-7
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• 2010

Science (natural science) is the systematic attempt to understand and interpret the nature phenomenon. For this reason, architects have used science to adapt nature to their design. With the rise of modern science, architecture became more closely related with science. Science available to develop new technology for architecture and it influenced architect's idea and concept. Symbolically, Architects use method or process of science to generate building form. The Rules of compositing particles in the chemistry or DNA (deoxyribonucleic acid) in the biology are used to generate a form of building. Literally, Architects use technology as a tool of science to improve physical performance of architecture. Like mathematical understanding of structure load enabled people to construct enclosure without columns or any of support system inside of architecture. Still natural phenomenon is not fully understood as science and science is still discovering a new phenomenon or changing its theory to adapt new discovery. New discovery or limitation of science influenced architecture throughout the history. This paper is to discuss how architectural theories are rest upon idea set forth by science. In addition, how technology as a tool of science has been utilized in architecture.