Plant Biotechnology Reports

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Arabidopsis cyclin D2 expressed in rice forms a functional cyclin-dependent kinase complex that enhances seedling growth

  • Oh, Se-Jun (School of Biotechnology and Environmental Engineering, Myongji University) ;
  • Kim, Su-Jung (School of Biotechnology and Environmental Engineering, Myongji University) ;
  • Kim, Youn Shic (School of Biotechnology and Environmental Engineering, Myongji University) ;
  • Park, Su-Hyun (School of Biotechnology and Environmental Engineering, Myongji University) ;
  • Ha, Sun-Hwa (National Institute of Agricultural Biotechnology, RDA) ;
  • Kim, Ju-Kon (School of Biotechnology and Environmental Engineering, Myongji University)
  • Received : 2008.07.07
  • Accepted : 2008.08.05
  • Published : 2008.11.30
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Abstract

D-class cyclins play important roles in controlling the cell cycle in development and in response to external signals by forming the regulatory subunit of cyclin-dependent kinase (CDK) complexes. To evaluate the effects of D-class cyclins in transgenic rice plants, Arabidopsis cyclin D2 gene (CycD2) was linked to the maize ubiquitin1 promoter (Ubi1) and introduced into rice by the Agrobacterium-mediated transformation method. Genomic deoxyribonucleic acid (DNA), ribonucleic acid (RNA), and Western blot hybridizations of the Ubi1:-CycD2 plants revealed copy number of transgene and its increased expression in leaf and callus cells at messenger RNA (mRNA) and/or protein levels. The H1 kinase assay using the immunoprecipitates of protein extracts from the Ubi1:CycD2 plants and nontransgenic controls demonstrated that the introduced Arabidopsis CycD2 forms a functional CycD2/CDK complex with an unidentified CDK of rice. Shoot and root growth was enhanced in the Ubi1:CycD2 seedlings compared with nontransgenic controls, together, suggesting that Arabidopsis cyclin D2 interacts with a rice cyclin-dependent kinase, consequently enhancing seedling growth.

Keywords

Acknowledgement

Supported by : Crop Functional Genomics Center

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