• Restorative Dentistry and Endodontics
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• 제24권1호
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• pp.180-186
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• 1999

Eugenol has been reported to reduce odontogenic pain and is known to have a structure similar to capsaicin, a potent stimulant of certain nociceptors. We have hypothesized that the analgesic effect of eugenol may be due, in part, to inhibition of capsaicin-sensitive nociceptors. To test this hypothesis, we evaluate whether eugenol inhibits capsaicin-sensitive release of immunoreactive calcitonin generated peptide(iCGRP) from bovine dental pulp. Freshly extracted bovine incisors were transported to the lab. on ice, Spilitted and pulp tissue was removed. The tissue was chopped into 200${\mu}m$ slices. Dental pulp was superfused(340 ${\mu}l/min$) in vitro with oxygenated Kreb's buffer. Eugenol and vehicle(0.02% 2-hydroxyl-${\beta}$-cyclodextrin) were administered prior to stimulation of pulp with capsaicin and iCGRP was measured by RIA. The results were as follows: 1. Administration of eugenol has no effect on basal release of iCGRP. 2. In the vehicle treated group, capsaicin evoked a 2.5-fold increase over basal iCGRP levels. 3. Administration of eugenol(600 ${\mu}M$) reduced capsaicin evoked release of iCGRP by more than 40%(153.4${\pm}$41.1% vs 258.9${\pm}$21.7%). 4. 2-hydroxylpropyl-${\beta}$-cyclodextrin of less than 0.02% is found to be an effective vehicle to dissolve eugenol without evoking iCGRP release from dental bovine pulp. These data indicate that eugenol inhibits pulpal capsaicin-sensitive fibers and suggest that intracanal medicament of eugenol may relieve pain, in part, by this mechanism.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2003년도 학술회의 논문집 정보 및 제어부문 B
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• pp.727-730
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• 2003

Electric pulp test is a method to examine the vitality of dental pulp using the electrical stimulation. During the pulp test, the current stimulates intradental nerve, and makes patients painful. Some studies were accomplished to measure the responses of subjects by stimulating over the sensory threshold. In this study, we examined the time delay between pain feeling and stimulation stop in clinical situation. And we measured the activated responses(EMG in anterior belly of digastric muscle, voice, and finger span). As a result, it was verified that the minimum and maximum delay was EMG and voice, respectively. By reducing the excessive stimulus time, the unnecessary pain can be minimized using EMG that has the minimum delay.

• Restorative Dentistry and Endodontics
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• 제48권1호
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• pp.2.1-2.12
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• 2023

Objectives: In this study, natural substances were introduced as primary dental pulp caps for use in pulp therapy, and the antimicrobial and cytotoxic properties of these substances were investigated. Materials and Methods: In this in vitro study, the antimicrobial properties of calcium-enriched mixture (CEM) cement, propolis, and propolis individually combined with the extracts of several medicinal plants were investigated against Enterococcus faecalis, Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus. Then, the cytotoxicity of each substance or mixture against pulp stem cells extracted from 30 primary healthy teeth was evaluated at 4 concentrations. Data were gathered via observation, and optical density values were obtained using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) test and recorded. SPSS software version 23 was used to analyze the data. Data were evaluated using 2-way analysis of variance and the Tukey test. Results: Regarding antimicrobial properties, thyme alone and thyme + propolis had the lowest minimum inhibitory concentrations (MICs) against the growth of S. aureus, E. coli, and P. aeruginosa bacteria. For E. faecalis, thyme + propolis had the lowest MIC, followed by thyme alone. At 24 and 72 hours, thyme + propolis, CEM cement, and propolis had the greatest bioviability in the primary dental pulp stem cells, and lavender + propolis had the lowest bioviability. Conclusions: Of the studied materials, thyme + propolis showed the best results in the measures of practical performance as a dental pulp cap.

• Journal of Oral Medicine and Pain
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• 제16권1호
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• pp.95-102
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• 1991

The purpose of this study carried into the Electric pulp test for having knowledge of the difference of each one's stimulant threshold in normal teeth, the stimulant threshold at 10:00 A.M. and 3:00 P.M. in the same teeth, the differendce of the stimulant threshold between dental caries and normal contralateral teeth. In this study, 27 students aged between 22 and 24 years were selected from a pool of students who are sttending school of dentistry, chosun university who did not possessed dental disease like the dental caries, periodontal disease and restoration etc., and 30 outpatients who possessed dental caries(++). The obtained results were as follows : 1. The stimulant thresholds of the premolars and canine were higher than that of the incisors. 2. The stimulant thresholds of the carious teeth were lower than that normal contralateral teeth. 3. There were no difference stimulant threshold at 10:00 A.M. and 3:00 P.M.

• 전자공학회논문지SC
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• 제41권6호
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• pp.43-49
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• 2004

치수 검사(pulp test)는 치아에 물리적 및 화학적 자극을 가하여 치수의 생활력(vitality) 여부를 판별하는 검사이다. 치과 임상에서 수행되는 검사 과정에서 피검자는 치아에 가해지는 역치 이상의 자극으로 인하여 큰 고통과 스트레스에 노출된다. 본 논문에서는 생활치수의 전기 치수 검사 시, 자극의 강도를 서서히 증가시켜 역치에 이르게 되면 나타나는 피검자의 동통반응으로 개구반사에 의한 악이복근의 근전도, 발성에 의한 음성 반응, 손가락의 움직임에 의한 반응을 각각 측정하였다. 또한 동통 반응이 발생하는 시점으로부터 자극이 차단될 때까지 피검자에게 필요이상으로 인가되는 과용 자극 시간을 측정하였으며, 과용자극 시간 측정 시 치수 검사기의 자극 차단 주체자에 따른 과용 자극 시간을 측정 분석하였다. 이러한 결과를 바탕으로 동통 반응에 의한 인체 반응 신호를 이용하여 치수검사기의 출력을 자동으로 차단하는 제어 스위치를 구성하였다. 피검자가 역치 자극을 느낀 후 나타나는 최초의 인체 반응의 10 ms 이내에 신속하게 검사기의 출력을 차단함으로써 과용자극 시간을 줄이고자 하였다.

• Journal of Periodontal and Implant Science
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• 제29권1호
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• pp.95-102
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• 1999

The purpose of this study was to observe histopathologically the influence of advanced periodontitis on pulp tissue, and to conclude the correlation between the results with clinical manifestations. The samples were teeth with over 7mm pocket depth and over 50% radiographic bone loss. These were diagnosed to have very poor prognosis and thus planned to be extracted. Those with any of following conditions were excluded from the samples, loss of vitality, periapical pathology, restoration or prosthesis, dental caries, and attrition or abrasion. It was because these conditions could affect pulp without any correlation with periodontal disease. For the experiment, 17 teeth from 11 patients were selected. Average age of patient was 47. Each tooth was examined for following categoris; pocket depth, gingival recession, electric pulp test, mobility, percussion test, sensitivity test. The extracted teeth were fixed buffered neutral formalin solution. It was decalcified using 4% nitric acid. Sliced histological samples observed using light microscope, for pulp status, and severeity of inflammation. 4 samples were excluded due to histologic sample discrepency. Thus 13 samples were subject to observation. 4 showed normal conditions. Focal reversable pulpitis was shown in 5 samples. Chronic pulpitis was observed 1 sample. Pulpal abscess was observed in 3 samples.

• Restorative Dentistry and Endodontics
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• 제41권3호
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• pp.202-209
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• 2016

Objectives: The purpose of this study was to investigate the involvement of TRPA1 in the cinnamaldehyde-induced pulpal blood flow (PBF) change in the feline dental pulp. Materials and Methods: Mandibles of eight cats were immobilized and PBF was monitored with a laser Doppler flowmetry at the mandibular canine tooth. To evaluate the effect of cinnamaldehyde on PBF, cinnamaldehyde was injected into the pulp through the lingual artery at a constant rate for 60 seconds. As a control, a mixture of 70% ethanol and 30% dimethyl sulfoxide (DMSO, vehicle) was used. To evaluate the involvement of transient receptor potential ankyrin 1 (TRPA1) in PBF change, AP18, a specific TRPA1 antagonist, was applied into the pulp through the Class V dentinal cavity followed by cinnamaldehyde-administration 3 minutes later. The paired variables of experimental data were statistically analyzed using paired t-test. A p value of less than 0.05 was considered as statistically significant. Results: Administration of cinnamaldehyde (0.5 mg/kg, intra-arterial [i.a.]) induced significant increases in PBF (p < 0.05). While administration of a TRPA1 antagonist, AP18 (2.5 - 3.0 mM, into the dentinal cavity [i.c.]) caused insignificant change of PBF (p > 0.05), administration of cinnamaldehyde (0.5 mg/kg, i.a.) following the application of AP18 (2.5 - 3.0 mM, i.c.) resulted in an attenuation of PBF increase from the control level (p < 0.05). As a result, a TRPA1 antagonist, AP18 effectively inhibited the vasodilative effect of cinnamaldehyde (p < 0.05). Conclusions: The result of the present study provided a functional evidence that TRPA1 is involved in the mechanism of cinnamaldehyde-induced vasodilation in the feline dental pulp.

• Restorative Dentistry and Endodontics
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• 제21권1호
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• pp.353-359
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• 1996

The purpose of this study was to investigate the concentrations of Leukotriene B4 in relation to the clinical symptom of pulpitis in human dental pulp. Pulps obtained from 3 groups of teeth: normal uniflamed teeth(N=22), asymptomatic teeth with deep caries or large restorations(N = 21) and symptomatic teeth with the clinical diagnosis of irreversible pulpitis(N = 15). Pulps were dissected from normal un inflamed teeth and extirpated from asymptomatic and symptomatic teeth during routine endodontic treatment and stored in liquid nitrogen ($-70^{\circ}C$). The levels of Leukotriene B4 in individual or pooled pulps were measured by radioimmunoassay and the mean levels of each group were compared statistically(Kruskall-Wallis oneway ANOVA test). The results were as followings : 1. In normal pulp, low levels of Leukotriene B4 were measured. 2. In pulps from asymptomatic and symptomatic teeth had significantly higher levels of Leukotriene B4 than normal pulps(p<0.01). 3. The levels of Leukotriene B4 in pulps from symptomatic teeth were significantly higher than those of pulps from asymptomatic teeth(p<0.01). These results suggest that Leukotriene B4 play a cretain role in inflammatory process of dental pulp and have a relationship with clinical symptoms of pulpitis.

• 대한치과의사협회지
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• 제57권10호
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• pp.569-578
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• 2019

The purpose of this retrospective study was to compare outcome of preformed stainless steel crown restorations according to depth of caries in primary molars. The study subjects consisted primary molars having dentin caries extending at least one-fourth of dentin without signs and symptoms of irreversible pulpitis. A total 295 primary molars received preformed stainless steel crown restorations without any base materials were included in this study. Survival rates were compared using a Kaplan-Meier analysis test. There was no significant difference between depth of caries and survival period in primary molars. And also there was no significant difference with or without pulp therapy. The results of this study suggest that neither the amount of caries nor pulp therapy affect the survival period of primary molars significantly. Proper restoration is more important to achieve sealing for stopping caries progression.

• Restorative Dentistry and Endodontics
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• 제45권4호
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• pp.48.1-48.11
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• 2020

Objectives: This systematic review aimed to identify mean oxygen saturation values (SpO₂) using pulse oximetry in permanent maxillary anterior teeth. Materials and Methods: The MEDLINE, Scientific Electronic Library Online, Cochrane Central Register of Controlled Trials, EMBASE, and Literatura Latino Americana em Ciências da Saúde electronic databases were searched. Combinations and variations of "oximetry" AND "dental pulp test" were used as search terms. Studies reporting means and standard deviations of SpO₂ values were included. Two reviewers independently extracted data following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses checklist. Heterogeneity was assessed using the I² statistic, and all analyses were performed using R software. Study quality was assessed using the Quality Assessment of Diagnostic Accuracy Studies-2 tool and the Newcastle-Ottawa scale. Results: Of the 251 studies identified, 19 met the eligibility criteria and were included (total sample, 4,541 teeth). In the meta-analysis, the mean SpO₂ values were 84.94% (95% confidence interval [CI], 84.85%-85.04%) for the central incisors, 89.29% (95% CI, 89.22%-89.35%) for the lateral incisors, and 89.20% (95% CI, 89.05%-89.34%) for the canines. The studies were predominantly low-quality due to the high risk of bias associated with the index test, unclear risk regarding patient selection, and concerns about outcome assessment. Conclusions: Although most studies were low-quality, the oxygen saturation levels in normal pulp could be established (minimum saturation, 77.52%). Despite the risk of bias of the included studies, the reference values reported herein are clinically relevant for assessments of changes in pulp status.