Restorative Dentistry and Endodontics

The Korean Academy of Conservative Dentistry (대한치과보존학회)
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Antimicrobial and cytotoxic properties of calcium-enriched mixture cement, Iranian propolis, and propolis with herbal extracts in primary dental pulp stem cells

  • Mohammad Esmaeilzadeh (Department of Pediatrics, Faculty of Dentistry, Shahid Beheshti University of Medical Sciences) ;
  • Shirin Moradkhani (Medicinal Plants and Natural Products Research Center, Department of Pharmacognosy and Pharmaceutical Biotechnology, School of Pharmacy, Hamadan University of Medical Sciences) ;
  • Fahimeh Daneshyar (Department of Pediatrics, Faculty of Dentistry, Hamadan University of Medical Sciences) ;
  • Mohammad Reza Arabestani (Department of Microbiology, School of Medicine, Hamadan University of Medical Sciences) ;
  • Sara Soleimani Asl (Endometrium and Endometriosis Research Center, Department of Anatomy, School of Medicine, Hamadan University of Medical Sciences) ;
  • Soudeh Tayebi (Department of Pediatrics, Faculty of Dentistry, Hamadan University of Medical Sciences) ;
  • Maryam Farhadian (Department of Biostatistics, School of Public Health and Research Center for Health Sciences, Hamadan University of Medical Sciences)
  • Received : 2022.05.06
  • Accepted : 2022.08.16
  • Published : 2023.02.28
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Abstract

Objectives: In this study, natural substances were introduced as primary dental pulp caps for use in pulp therapy, and the antimicrobial and cytotoxic properties of these substances were investigated. Materials and Methods: In this in vitro study, the antimicrobial properties of calcium-enriched mixture (CEM) cement, propolis, and propolis individually combined with the extracts of several medicinal plants were investigated against Enterococcus faecalis, Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus. Then, the cytotoxicity of each substance or mixture against pulp stem cells extracted from 30 primary healthy teeth was evaluated at 4 concentrations. Data were gathered via observation, and optical density values were obtained using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) test and recorded. SPSS software version 23 was used to analyze the data. Data were evaluated using 2-way analysis of variance and the Tukey test. Results: Regarding antimicrobial properties, thyme alone and thyme + propolis had the lowest minimum inhibitory concentrations (MICs) against the growth of S. aureus, E. coli, and P. aeruginosa bacteria. For E. faecalis, thyme + propolis had the lowest MIC, followed by thyme alone. At 24 and 72 hours, thyme + propolis, CEM cement, and propolis had the greatest bioviability in the primary dental pulp stem cells, and lavender + propolis had the lowest bioviability. Conclusions: Of the studied materials, thyme + propolis showed the best results in the measures of practical performance as a dental pulp cap.

Keywords

References

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