• Title/Summary/Keyword: Densitometry

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Formation of Luteoskyrin by Penicillium islandicum (Penicillium islandicum에 의한 황변미독(黃變米毒) Luteoskyrin의 생성(生成))

  • Kim, Yong-Hwa;Lee, Su-Rae
    • Korean Journal of Food Science and Technology
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    • v.10 no.1
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    • pp.46-51
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    • 1978
  • One of yellowed rice toxins, luteoskyrin, was investigated with respect to its identification, quantitation and producibility by Penicillium islandicum isolated from deteriorated rice. 1) Luteoskyrin was best resolved by thin-layer chromatography with silica gel G plate impregnated with 0. 5 N oxalic acid and acetone : n-hexane : water (6 : 3 : 1.5, upper layer) solvent system. The isolated yellow spot showed maximum absorption bands at 426 and 448 nm and changed to purple color upon exposure to sunlight for $2{\sim}3$ hours. 2) Detection limit for luteoskyrin was 4 ppm in elution-colorimetry and 0.1 ppm in densitometry after TLC. Assuming that the tolerance for luteoskyrin in rice is set below 3.68 ppm, densitometry is usable for its screening in grain samples 3) Producibility of luteoskyrin by Pen. islandicum was shown to be 11 mg/g mycelial mat in liquid culture and 40 mg/g autoclaved rice.

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Protein Composition of Domestic and Glyphosate-Tolerant Soybean (국내산 및 제초제 내성 콩의 단백질 조성 분석)

  • Wei, Chun-Hua;Sok, Dai-Eun;Yang, Yun-Hyoung;Oh, Sang-Hee;Kim, Hyoung-Chin;Yoon, Won-Kee;Kim, Hwan-Mook;Kim, Mee-Ree
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.35 no.4
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    • pp.470-475
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    • 2006
  • In order to elucidate the differences of protein profiles among soybean cultivars, the protein composition of three conventional domestic soybean cultivars and two imported ones including glyphosate-tolerant HS2906 was analyzed by total nitrogen measurement, amino acid analysis and PAGE/densitometry. There were no statistically significant differences in the levels of any amino acid, including aromatic amino acids, between glyphosale-tolerant soybean and the conventional soybean WS82. In the extraction of protein, the SDS/buffer system was more efficient than the defatting/water system. The SDS-PAGE/densitometry analysis showed that there was a similar profile of proteins among cultivars, although the amount of total protein ranged from 380.2 mg/g to 423.9 mg/g. In addition, there was no discernable difference of protein profile between glyphosate- tolerant soybean (total protein amount, 380.2 mg/g) and the conventional soybean WS82 (390.2 mg/g), although the amount of ${\beta}$-conglycinin (55 kDa) was lower in glyphosate-tolerant soybean. Meanwhile, the amount of 25 kDa protein was greater in domestic soybean cultivars than imported ones. Thus, normal PAGE/ densitometry method would be useful to analyze the difference in protein profiles of soybean proteins, and furthermore Evaluate the protein profile of proteins between GMO and conventional soybean.

Comparison of the Values of Bone Mineral Density Between DEXAs (DEXA 측정기 간 골밀도 값 비교)

  • Lee, In-Ja
    • Journal of radiological science and technology
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    • v.34 no.4
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    • pp.271-276
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    • 2011
  • Statistical analysis was performed on the patients who took bone densitometry using Lunar and Hologic equipments for 5 months from January $1^{st}$ 2010 to May $30^{st}$, 2010. Patients consisted of 50 in their 40s, 100 in 50s and 50 in 60s. In addition, the results from bone densitometry were carried out on the same subject with two equipments on the same day. In case of 200 subjects, who received the bone densitometry with two equipments, the average age was 54.5 and 54.4 years old, respectively. There was no difference. The T- score of Lunar equipment was $-1.377{\pm}1.221$ and that of Hologic equipment was $-1.806{\pm}1.123$. The T-score of Lunar equipment was measured higher than that of Hologic equipment. T-test was conducted to determine the equality of the mean of two groups with 200 patients. Since the pvalue was 0.000, the value of bone mineral density was significant in two equipments. Furthermore, the patients, who were diagnosed by Lunar, showed more 'normal' and who were diagnosed by Hologic, showed more osteoporosis, which cartegorized by WHO, such as normal, osteopenia, and osteoporosis. Thus, compared results of bone densitometry on lumbar spine L1 - L4 of four normal people with the same equipment showed that T- score of Lunar equipment was $-0.4{\pm}1.2$, and T- score of Hologic equipment was $-1.1{\pm}1.5$. It showed the higher T- score was measured in Lunar equipment as well. Therefore, the correction factor should be considered to use, since T- scores are different between two equipments.

The Study of Quality Control for Package Printing (포장 인쇄의 인쇄적성 향상에 관한 연구)

  • Lee, Man-Gyo;Ha, Young-Baeck;Youn, Jong-Tae
    • Journal of the Korean Graphic Arts Communication Society
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    • v.23 no.1
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    • pp.53-63
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    • 2005
  • The quality of the print can be specified through the quality of the coloring, the reproduction of fine structures and the range of tone values. Also, package printed quality are controlled by this points. In this paper, densitometry method was used for printed quality. By densitometry we can get the print density and the parameters of each conditions that characterize halftone printing, such as dot gain and relative printing contrast. Also, we have proposed the optimizes range of the parameters such as density, dot gain etc. to the package printed printability control.

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Quantitative Analysis of Orcinol and Acute Toxicity of Gyrophora esculenta (석이중 오르시놀 정량 및 급성독성)

  • 최혁재;김남재;김동현
    • YAKHAK HOEJI
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    • v.45 no.2
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    • pp.169-179
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    • 2001
  • In previous study, Gyrophora esculenta showed significant inhibitory effect on $\alpha$-glucosidases in vitro and blood glucose elevation in vivo. In the isolating process of active substance, orcinol was separated from Gyrophora esculenta. Orcinol is known to be toxic, therefore, in this study, it was analysed by the TLC densitometry method for quantitative determination from Gyrophora esculenta. The average amount of orcinol of Gyrophora esculenta was 0.2%. For the purpose of removing orcinol, the water extract of Gyrophora esculenta was sequentially fractionated by organic solvents, and the acute toxicity of each fraction was assessed in mice. Among them, the LD50 of butanol fraction was 1.19 g/kg(p.o.) and the weight increase of mice in that group was somewhat retarded.

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Incidentally detected abnormal finding of femoral bone density image due to treatment of postoperative lymphatic leakage

  • Han-Kyung Seo;Do-Cheol Choi;Jae-Yung Jeong;Cheol-Min Shim;Yung-Hwan Jeong
    • The Korean Journal of Nuclear Medicine Technology
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    • v.28 no.1
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    • pp.13-17
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    • 2024
  • Bone Mineral Densitometry (BMD) values is determined numerically with T or Z-score while the image is not considered in the interpretation. Abnormal finding was observed in the left femoral bone density image of a 64-year-old female patient who underwent a left kidney transplantation. This was due to the ethiodized oil used for embolization in the treatment of lymphatic leakage. Radiologic technologists should not only monitor changes of BMD values, but keep in mind to assess the changes in the image to provide accurate information to the patient.

Total Body Fat Estimated by Means of Densitometry and Skinfold Thickness Method in Middle Aged Housewives (밀도법 및 피부두겹법에 의한 중년부인의 총지방량 측정)

  • Choi, D.K.;Shin, H.S.;Hwang, E.R.
    • The Korean Journal of Physiology
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    • v.2 no.1
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    • pp.89-93
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    • 1968
  • Total body fat measurements were performed in 342 housewives. In 44 of them(age 38.4 yr.) both the densitometry and skinfold thickness methods were made and regression equations between skinfold and total body fat were derived. In the 298 housewives(age 35.0 yr.) skinfold thickness at four sites (arm, back, waist, abdomen) were measured and total body fat was calculated. The following results were obtained. 1. The data in 44 subjects by means of densitometry and skinfold thickness were: body weight: 51.8 kg, body length: 153.4 cm, body surface area: $1.47\;m^2$, body volume: 50.2 l, body density: 1.0334 kg/l, total body fat: 25.2% body weight, mean skinfold thickness at four sites 14.9 mm. 2. There were correlations of high degree between skinfold thickness and fat (%), fat (kg), and body density. The correlation coefficients were r=0.767, r=0.846, r= -0.765, respectively. Subsequently, the following regression equations were obtained. %fat=$0.39{\times}Mean$ skinfold thickness(mm)+19.36 Fat(kg)=$0.414{\times}Mean$ skinfold thickness(mm)+7.01 Body density=$-0.00099{\times}Mean$ skinfold thickness(mm)+1.0489 3. In 298 subjects mean skinfold thickness of four sites was 20.6mm and total body fat was calculated as 27.4% body weight from the above equation.

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Protein Profiles of Major Korean Rice Cultivars

  • Wei, Chuanha;Kwon, Oh-Yun;Liu, Xi-Wen;Kim, Hyoung-Chin;Yoon, Won-Kee;Kim, Hwan-Mook;Kim, Mee-Ree
    • Preventive Nutrition and Food Science
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    • v.12 no.2
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    • pp.103-110
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    • 2007
  • The protein profiles among Korean rice cultivars were assessed by total protein determination, solubility fractionation, SDS-PAGE analysis and scanning densitometry. In the extraction of protein, the SDS/urea system at a neutral pH was more efficient than that at alkaline pH. The determination of total protein showed that the protein content was similar among cultivars, ranging from 87.9 to 92.7 mg/g dry weight. Additionally, the water/NaCl-soluble protein fraction, containing 14${\sim}$16 kDa albumin and 22 kDa globulin ${\alpha}$-globulin, was also similar among cultivars, with a range of 9.94 to 11.98 mg/g dry weight. The SDS-PAGE/densitometry of total protein showed that there was no discernable difference in proteins of higher molecular weights among various cultivars, whereas the amount of lower molecular weight proteins (14${\sim}$16 kDa) is somewhat variable among cultivars. Furthermore, SDS-PAGE analysis of water/NaCl-soluble and propanol-soluble fractions indicates that there is a discernible change in the content of albumin, globulin or prolamin among cultivars. Thus, the PAGE/densitometry method, preceded by solubility fractionation, is useful for examining differences in protein profiles of rice cultivars.

A Study on the Body Composition in Korean Basketball Players by Dual Energy X -ray Absorptiometry (DEXA사에 의한 일부 남자농구선수들의 체구성에 관한 연구 -체지방량, 체구성, 비율, 체조직 구성 비율, 골광물질함량과 골밀도를 중심으로-)

  • 박경래;강동원;최중명;박순영
    • Korean Journal of Health Education and Promotion
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    • v.15 no.1
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    • pp.179-194
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    • 1998
  • This study focused on the body composition of Korean Basketball Players in Dual X -ray Absorptiometry. The principal subjects of this study were 10 Basketball Players who participated in the process of Dual Energy X -ray Absorptiometry at K.H. University Hospital for 8 months from Sept. 1. 1996 to April, 30. 1997. On the basis of the these measurements, the authors calculated physical indices and total fat percent. On the results of bone densitometry, the author analyzed body fat weight, body composition according to body position, bone mineral density and discerned the difference between the fatness which was calculated indirectly and which gained from the bone densitometry. 1. General Characteristics The mean age of the subjects was 20.4$\times$1.35 year. 2. Physical. Measurement The mean physical growth and development of the subjects were superior to standard value of the average Koreans 3. Body Fat by Physical Measurement Body surface area was 2.019$\times$0.111$m^2$, body volume was 74.4$\times$7.2$\ell$, body density was 1.041$\times$0.007$\ell$/kg, and body fat percent was 24.9$\times$2.9%. 4. Body Fat by Bone Densitometry Total body fat percent was 15.17$\times$2.19% and according to body position that of upper limb was 1.62%, that of lower limb was 5.55%, that of trunk was 7.06% and that of head was 1.05%. There was significant difference between the amounts of body fat from the methods used in this study; that from physical measurements and from bone densitometry method could be said most desirable. 5. Body composition According to Body Position Trunk was highest at 46.7%, lower limb was 36.0%, upper limb was 10.6%, and head was 6.7% in order. 6. Bone Mineral Content and Bone Density In bone mineral amount by body position, that of upper limb was 466.9$\times$46.4g, that of lower limb was 1,424.1$\times$154.0g, that of trunk was 1,343.0$\times$150.3g, and total bone mineral content was 3,786.8$\times$348.4g(4.78$\times$0.13%). Bone mineral density by body position, that of upper limb was 0.758$\times$0.072g/$\textrm{cm}^2$, that of lower limb was 1,342$\times$0.095g/$\textrm{cm}^2$, that of trunk was 1,169$\textrm{cm}^2$0.082g/$\textrm{cm}^2$, that of head was 1,742$\times$0.154g/$\textrm{cm}^2$ and total bone mineral density was 1,204$\times$0.077g/$\textrm{cm}^2$.

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