• 미생물학회지
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• 제27권3호
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• pp.245-249
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• 1989

Bacillus licheniforntis에서 분비된 단백질 추출물에서 ammonium sulfate fractionation과 Sephadex G-75젤 여과 크로마토그라피를 사용하여 부분 정제된 2개의 서로 다른 활성을 갖는 단백질 분해 효소를 얻었다. 단백질 분해 효소 type l은 카제인에 대해 비교적 높은 활성을 보이며 organotluoride와 EOTA에 의한 억제에 매우 민감했다 이 효소의 최적 pH는 7.5였으며 $75^{\circ}C$에서 빠르게 변성되었다. 단백질 분해효소 type II는 type I에 비해 낮은 카제인 분해 활성을 보이며 ImM PMSF와 10mM EOTA용액과 함께 $37^{\circ}C$에서 30분 간 반응시키면 역사 활성이 억제되었다 한편 이 효소 는 pH 8.0에서 최대 활성을 나타내고 $71^{\circ}C$ 에서 상대적으로 늦게 불환성화 되었다.

• IEIE Transactions on Smart Processing and Computing
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• 제3권1호
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• pp.28-34
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• 2014

Recently, ultrasound therapy has become a new and effective treatment for many brain diseases. Therefore, skull-mimicking phantoms have been developed to simulate the skull and brain tissue of a human and allow further research into ultrasound therapy. In this study, the suitability of various skull-mimicking materials(HDPE, POM C, Acrylic) for studies of brain-tumor treatments was evaluated using focused ultrasound. The acoustic properties of three synthetic resins were measured. The skull-mimicking materials were then combined with an egg white phantom to observe the differences in the ultrasound beam distortion according to the type of material. High-intensity polyethylene was found to be suitable as a skull-mimicking phantom because it had acoustic properties and a denatured-area shape that was close to those of the skull,. In this study, a skull-mimicking phantom with a multi-layer structure was produced after evaluating several skull-mimicking materials. This made it possible to predict the denaturation in a skull in relation to focused ultrasound. The development of a therapeutic protocol for a range of brain diseases will be useful in the future.

• 한국식품영양과학회지
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• 제23권1호
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• pp.110-115
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• 1994

To investigate the effect of potato lipoxygenase on the farinograph characteristics of wheat flour dough, composite flours containing enzyme-active potato flor (EPF) and hot-ar dried potato flour(HPF) were used. EPF was made by freeze-drying potato tuber. DPF (denaturated potato flour) was prepared by holding EPF at 8$0^{\circ}C$ for 18 hr in a dry oven. The potato flours were added to wheat flour at a level of 10% , respectivley. EPFB (enzyme-active potato flour blends, 90% wheat flour +105 enzyme -active potato flour) containing lipoxygenase activity gave higher farinogram peak time and higher stability values, lower MTI (mixing tolerance index ) and lower weakness values than those of HPFB(hot-air potato flour blends, 90% wheat flour + 10 % hot-air potato flour). Moreover, then lipoxygenase was added to DPFB(denatured potato flour blends , 90% wheat flour + 10% denatured potato flour) at a level of EPFB, it resulted in increasing stability, peak time and decreasing MTI , weakness at a level of EPFB. When the lipoxugenase was added to wheat flour with fumaric acid at alevel of 6.5 $\times$ 10units/g flour, lipoxygenase overcame the deleterious effects that fumaric acid including activated double-bond compounds have at mixing stability. Also the addition of liposxygenase with linoleic acid to defatted wheat flour resulted in the increase in stability and decrease in MTI value compared with those of linoleic acid and defatted wheat flour.

• 대한화학회지
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• 제29권6호
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• pp.646-650
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• 1985

RNase A에 對한 RNA의 加水分解反應性은 spermine에 의하여 抑制되었다. Spermine의 濃度가 增加함에 따라 RNase의 活性度는 점점 減少하다가 plateau에 到達되었다. 同一한 條件에서 spermine의 濃度가 增加함에 따라 RNA의 粘性度는 점점 增加되어 RNase의 活性度에서의 spermine의 依存性과 같이 plateau에 이르게 되었다. RNase A에 對한 RNA의 加水分解反應性에 미치는 spermine의 抑制效果는 denatured(變性) DNA에 의하여 救濟되나 native(本性) DNA에 의하여서는 無關하였다. 이들 實驗結果는 spermine에 의하여 RNA의 分子間 aggregation이 일어나며 그로 因하여 RNA의 RNase A에 對한 加水分解反應性이 抑制될 수 있음을 보여준다.

• 한국건축시공학회:학술대회논문집
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• 한국건축시공학회 2017년도 추계 학술논문 발표대회
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• pp.118-119
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• 2017

The combined waterproofing method is an excellent method to overcome the disadvantages of the single waterproofing method by composing two or more materials to complement each other, but it is a method that can cause defects such as separation and peeling between materials due to the heterogeneity of the applied two materials. In order to improve this, in this study, we aimed to develop a technology for inducing chemical unification between materials through a urea reaction with a coating material applied on the lower side by laminating a nonwoven fabric treated with a modified amine on the back surface of the sheet material, The adhesion performance test was carried out with the presence or absence of denatured amine treated nonwovens as variables. As a result of the test, it was confirmed that the adhesion performance of the specimen to which the modified amin - treated nonwoven fabric was applied was improved by about 60% or more as compared with the specimens not having the denatured amine treated specimen.

• Journal of Plant Biology
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• 제13권2호
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• pp.11-14
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• 1970

Identification and observations of leucine aminopeptidase (LAP) and multiple molecular forms of the enzyme, isozymes, were made with a technique of starch-gel electrophoresis for various legume pollen. Plants tested other than Leguminosae demonstrated either no indication of the presence or at least tract of enzymes and the isozymes, although all legume pollen tested showed strong LAP patterns. The electrophoretic patterns of LAP failed to be shown if the extracts were heated or otherwise denatured. Extent of zymogrammatic appearance of LAP and the isozymes were characteristic of a species.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2000년도 추계학술발표대회 및 bio-venture fair
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• pp.711-712
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• 2000

DNA probe assay comprising a microwell as' solid matrix for the immobilization of streptavidin (SA) and an oligonucleotide with covalently bound fluorecein as detection probe was developed. The insolubilized SA captured the biotinylated DNA product of polymerase chain reaction (PCR), and the product was denatured under a basic condition. The remaining single-stranded DNA on the solid surface was hybridized with the probe for signal generation that was performed based on enzyme-linked immuno -reactions.

• 유기물자원화
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• 제6권1호
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• pp.43-52
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• 1998

본 연구는 피혁 폐기물로부터 크롬 이온을 분리한 상태로 Collagen을 추출할 수 있는 최적 조건에 관한 것이다. Collagen 추출에 있어 온도, pH, 그리고 알칼리 용액 농도의 영향을 실험했다. 실험결과 점도측정에 의해 초기 변성온도는 $25^{\circ}C$, 완전 변성온도는 $31.5^{\circ}C$였다. Collagen 추출의 최적조건은 가용화 온도 $15^{\circ}C{\sim}20^{\circ}C$, pH 1.5, 알칼리 용액 3%였다. PH 1.5에서 크롬 이온이 거의 분리되었다. 탄닌 폐기물로부터 크롬 이온의 분리효율은 99.5% 이상이었다. 피혁 폐기물로부터 조단백질의 추출율은 89.%이었다. 추출된 조단백질내에 hydroxyprolne과 collagen의 함량은 각각 8.53%, 63.62%였다.

• 한국의류학회지
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• 제20권4호
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• pp.655-666
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• 1996

The effect of protease (subtilisin Carlsberg) on the removal of hemoglobin as protein soil was studied. The relation between the renloval and the hydrolysis of hemoglobin by subtilisin Carlsberg was discussed. The soiled babric was prepared by spotting of hemoglobin solution evenly on the cotton fabric and was denatured by steaming. The soiled fabric was washed by using Terg-0-Tometer at various conditions. The removal efficiency was evaluated by analysis of protein on the fabrics before and after washing by means of copper-Folin method. 1. The removal of hemoglobin was increased in proportion to increasing of the enzyme concentration up to a certain point, but it began to decrease above the point. 2. The hemoglobin was removed effectively by adding of subtilisin Carlsberg, and more effectively removed by adding of AOS in the enzyme solution. 3. The removal of hemoglobin deviated from the first order reaction in detergency. 4. The renloval of hemoglobin was highest at $50^{\circ}C$ in detergency, Even at low temperature the removal efficiency of enzyme was relatively higher compared with the hydrolysis of hemoglobin by the enzyme. However the removal of hemoglobin was apparently decreased with the increase of temperature over $60^{\circ}C$. 5. The removal of hemoglobin was relatively high at pH 7.0~8.0 and increased continuously with the increase of pH in detergency 6. In detergency, the removal mechanism of hemoglobin by subtilisin Carlsberg could be explained as follows: Fisrt of all, the enzyme hydrolyzed hemoglobin substrates partially by forming E-S complex at the surface of hemoglobin on the cotton fiber, and decomposed cooperative binding of hemoglobin. Subsequently, the fragments of hemoglobin were easily removed by washing. According as the enzyme penetrated to inner part of hemoglobin gradually, the hemoglobin on the cotton fiber was effectively removed by the repetition of these process. The removal of hemoglobin was more effectively increased by adding both the enzyme and AOS in the washing solution. Therefore, it was regarded that AOS molecules were adsorbed at the hydrophobic surface of denatured hemoglobin, subsequently, decomposed more effectively cooperative binding of hemoglobin, and the fragments of hemoglobin were removed more efficiently by means of the interfacial reaction of AOS.

• Journal of Pharmaceutical Investigation
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• 제39권6호
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• pp.423-429
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• 2009

Liposomes have been used as one of the efficient carriers for drug delivery. In this study, anionic liposomes of which surface was modified by using both electrostaic interaction between anionic liposomes and cationically charged BSA molecules at lower pH than isoelectric point (pI) of BSA and denaturation of the BSA-coated liposomes by thermal treatment. The thermally denatured BSA-coated liposomes (DBAL) had mean particle diameter of 125.2${\pm}$1.7 nm and zeta potential value of -22.4${\pm}$4.5 mV. Loading efficiency of model drug, doxorubicin (DOX), into liposomes was 83.0${\pm}$2.6%. Results of in vitro stability study of DBAL in blood plasma showed that the mean particle diameter of DBAL 400 did not increase in blood plasma and adsorption of plasma protein was much less than plain or anionic liposomes. Intracellular uptake of DBAL 400 evaluated by confocal microscopy observation was higher than that of PEG liposomes.