Salmonella species 검출용 DNA Probe 분석시스템 고안

  • 이웅희 (고려대학교 생명공학원, 바이오센서 시스템공학 연구실) ;
  • 백세환 (고려대학교 생명공학원, 바이오센서 시스템공학 연구실)
  • Published : 2000.11.09

Abstract

DNA probe assay comprising a microwell as' solid matrix for the immobilization of streptavidin (SA) and an oligonucleotide with covalently bound fluorecein as detection probe was developed. The insolubilized SA captured the biotinylated DNA product of polymerase chain reaction (PCR), and the product was denatured under a basic condition. The remaining single-stranded DNA on the solid surface was hybridized with the probe for signal generation that was performed based on enzyme-linked immuno -reactions.

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