• Journal of fish pathology
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• v.6 no.2
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• pp.93-101
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• 1993

The RNAI mutation of Saccharomyces cerevisia is a recessive and temperature sensitive lethal mutation which interferes with the production of mRNA, rRNA, and tRNA. However, the precise role of RNAI gene have not been revealed until yet. We have cloned rna1-1 mutant gene from rna1-1 mutant yeast strain(R49 ; trpl, ura3-52, rna1-1). The 3.4kb BglII fragment of wild type RNAI clone(81-2-6) contains whole RNAI gene. The genomic southern blotting with BglII digested R49 genomic DNA as a probe shows the unique and identical band with wild type 3.4kb BglII fragment. Therefore, We prepared partial BglII genomic library(3~4kb BglII fragments) into BamH I site of pUC19. The rna 1-1 mutant clone was screened with Digoxigenin(DIG)-lableled probe by high density colony hybridization. The 5'-flanking region of rna1-1 gene was sequenced by dideoxy chain termination method. The 5'-flanking sequence of RNAI gene contains three TATA-like sequence ; TAATA, TATA and TTTTAA at position of -67, -45, and -36 from first ATG codon respectively. The 5'-flanking region of wild type RNA I gene from ATG codon to -103nt was deleted with Bal31 exonuclease digestion, generating $pUC{\Delta}$/RNA I. After constructing $pYEP{\Delta}RNA$ I (consists of -103nt deleting RNA I gene, URA3 gene, $2{\mu}m$ rep. origin), pYEPrna1-1(consists of Xba I fragment of pUCrna1-1. URA3 gene, $2{\mu}m$ rep. origin), and pYEPRNAI. each plasmid was transformed into host strain(trpl, ura3-52, rna1-1) by electroporation, respectively. Yeast transformant carrying $pYEP{\Delta}RNA$ I did not complement the thermal sensitivity of rna1-1 gene. It means that TATA-like sequences in 5'-flanking region is not TATA sequence for transcribing RNAI gene and there may be other essential sequence in upstream region for the transcription of RNAI gene.

• Journal of Life Science
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• v.26 no.9
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• pp.991-998
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• 2016

NF-κB acts as a critical transcription factor for the survival of cells via the induction of antiapoptotic genes. Constitutive activation of NF-κB in many types of solid tumors suggests that the inhibition of NF-κB might prevent or inhibit tumorigenesis. Although a number of studies demonstrated that Hsp70 regulated NF-κB activity, the exact mechanism is not clear. This study investigated the functional relationship of Hsp70 and IKKγ in the regulation of NF-κB activation using expression plasmids of components of the IKK complex. Wild-type and deletion mutants of IKKγ were expressed together with Hsp70, and the combined regulatory effect of Hsp70 and IKKγ on NF-κB activation was assayed. Hsp70 suppressed the activation of NF-κB in a reporter plasmid assay. Hsp70 also suppressed the phosphorylation and degradation of IκBα. The suppressive effect of Hsp70 on NF-κB activation was synergistically elevated by IKKγ. The N-terminal IKKβ binding site, C-terminal leucine zipper, and zinc finger domains of IKKγ were not necessary for the suppressive effect. Furthermore, Hsp70 and IKKγ synergistically suppressed the induction of COX-2 expression by lipopolysaccharides in RAW264.7 cells. These results suggest that overexpression of Hsp70 and IKKγ may be a strategic method for inhibition of NF-κB and related diseases.

• Journal of Internet Computing and Services
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• v.6 no.6
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• pp.13-22
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• 2005

This paper aims to develop a system for converting web contents to mobile contents that can be used on mobile devices. Since web contents generally consist of pop-up ad windows, a bunch of unnecessary images and useless links, it is difficult to efficiently display them on common mobile devices that have lower bandwidth and memory, as well as much smaller screen, than the online environment. It is also troublesome for mobile device users to directly access contents. Thus, there has been a great demand for a new method for extracting useful and adequate contents from web documents, and optimizing them for use on mobile phones, In the paper, a system based on WAP 2,0 and XHTML Basic, which is a content creation language adopted for WAP 2,0, has been suggested. The system is designed to convert web contents by using the conversion rules of the existing filtering method after making the size of web documents smaller. The adopted conversion rules use the XHTML Basic's module units so that modification and deletion can be carried out with ease. In addition, it has been defined in a XSL document written in XSLT to maintain the extensibility of conversion and the validity of documents, In order to allow it to efficiently work together with WAP l.X's legacy services, the system has been built in a way that can have modules, which analyze information about CC/PP profiles and mobile device headers.

• IMMUNE NETWORK
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• v.1 no.2
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• pp.151-161
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• 2001

Background: Inactivation in p53 tumor suppressor gene through a point mutation and deletion is one of the most frequent genetic changes found in human cancer, with 50% of an incidence. This high rate of mutation mostly suggests that the gene plays a central role in the development of cancer and the mutations detected so far were found in exons 5 to 8. Mutation of p53 locus produced accumulation of abnormal p53 protein, and negative regulation of cell proliferation and transcriptional activation as a suppressor of transformation were lost. In addition, inhibition of its normal cellular function of wild-type by mutant is an important step in tumorigenesis. Method: 4 colon cancer cell lines (SNU C1, C2A, C4, C5) were examined for mutation in exons 5 to 8 of the p53 tumor suppressor gene by PCR-SSCP analysis and expression pattern by western blotting and immunoprecipitation. p53-mediated transactivation ability were examined by CAT assay and base substitution of p53 in SNU C2A cell were detected by DNA sequencing. Results: 1) SNU C2A cell and SNU C5 cell were detected mobility shifts each in exon 5 and exon 7 of p53 gene by the PCR-SSCP method, implicating being of p53 mutation. 2) 3 colon cancer cell lines (SNU C1, SNU C2A, SNU C5) expressed wild type and mutant type p53 protein. 3) In northern blot experiment, SNU C2A and SNU C5 cell expressed high level of p53 mRNA. 4) Results of p53-mediated transactivation in colon cancer cell lines by CAT assay represented only SNU C2A cell has transcriptional activity. 5) DNA sequencing in SNU C2A cell showed missense mutation in codon 179 of one allele, histidine to arginine and wild type p53 in the other allele. Conclusion: Colon cancer cell lines showed correlation with mutation in p53 gene and accumulation of abnormal p53 protein. Colon cancer cell SNU C2A retained p53-mediated transactivation as heterozygous p53 with one mutant allele in 179 codon and the other wild-type allele.

• Clinical and Experimental Reproductive Medicine
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• v.43 no.1
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• pp.9-14
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• 2016

Objective: Autophagy contributes to the clearance and recycling of macromolecules and organelles in response to stress. We previously reported that vitrified mouse oocytes show acute increases in autophagy during warming. Herein, we investigate the potential role of Atg7 in oocyte vitrification by using an oocyte-specific deletion model of the Atg7 gene, a crucial upstream gene in the autophagic pathway. Methods: Oocyte-specific Atg7 deficient mice were generated by crossing Atg7 floxed mice and Zp3-Cre transgenic mice. The oocytes were vitrified-warmed and then subjected to in vitro fertilization and development. The rates of survival, fertilization, and development were assessed in the Atg7 deficient oocytes in comparison with the wildtype oocytes. Light chain 3 (LC3) immunofluorescence staining was performed to determine whether this method effectively evaluates the autophagy status of oocytes. Results: The survival rate of vitrified-warmed $Atg7^{f/f}$;Zp3-Cre ($Atg7^{d/d}$) metaphase II (MII) oocytes was not significantly different from that of the wildtype ($Atg7^{f/f}$) oocytes. Fertilization and development in the $Atg7^{d/d}$ oocytes were significantly lower than the $Atg7^{f/f}$ oocytes, comparable to the $Atg5^{d/d}$ oocytes previously described. Notably, the developmental rate improved slightly in vitrified-warmed $Atg7^{d/d}$ MII oocytes when compared to fresh $Atg7^{d/d}$ oocytes. LC3 immunofluorescence staining showed that this method can be reliably used to assess autophagic activation in oocytes. Conclusion: We confirmed that the LC3-positive signal is nearly absent in $Atg7^{d/d}$ oocytes. While autophagy is induced during the warming process after vitrification of MII oocytes, the Atg7 gene is not essential for survival of vitrified-warmed oocytes. Thus, induction of autophagy during warming of vitrified MII oocytes seems to be a natural response to manage cold or other cellular stresses.

• Journal of the Korea Society of Computer and Information
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• v.17 no.1
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• pp.31-40
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• 2012

u-GIS DSMSs have been researched to deal with various sensor data from GeoSensors in ubiquitous environment. Also, they has been more important for high availability. The data from GeoSensors have some characteristics that increase explosively. This characteristic could lead memory overflow and data loss. To solve the problem, various load shedding methods have been researched. Traditional methods drop the overloaded tuples according to a particular criteria in a single server. Tuple deletion sensitive queries such as aggregation is hard to satisfy accuracy. In this paper a dual processing load shedding method is suggested to improve the accuracy of aggregation in clustering environment. In this method two nodes use replicated stream data for high availability. They process a stream in two nodes by using a characteristic they share stream data. Stream data are synchronized between them with a window as a unit. Then, processed results are merged. We gain improved query accuracy without data loss.

• Journal of Internet Computing and Services
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• v.18 no.1
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• pp.11-20
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• 2017

Recently, developments in the various fields of cloud computing technology has been utilized. Whereas the demand for cloud computing services is increasing, security threats are also increasing in the cloud computing environments. Especially, in case when the hosts interconnected in the cloud environments are infected and propagated through the attacks by malware. It can have an effect on the resource of other hosts and other security threats such as personal information can be spreaded and data deletion. Therefore, the study of malware analysis to respond these security threats has been proceeded actively. This paper proposes a type of attack clustering method of Zeus botnet using the k-means clustering algorithm for malware analysis that occurs in the cloud environments. By clustering the malicious activity by a type of the Zeus botnet occurred in the cloud environments. it is possible to determine whether it is a malware or not. In the future, it sets a goal of responding to an attack of the new type of Zeus botnet that may occur in the cloud environments.

• Korean Journal of Medicinal Crop Science
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• v.22 no.6
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• pp.429-434
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• 2014

This study describes the efficient method for the discrimination of 'Cheonryang' in Panax ginseng Meyer using a STS primer. A total of 208 STS primers were applied to polymerase chain reaction (PCR) amplification for discriminating Korean ginseng cultivars. Co-dominant polymorphic band patterns were generated with two primers, MFGp 0019, MFGp 0248, and successful identification of 'Cheonryang' was achieved from out of 11 Korean ginseng cultivars. Two different sizes of DNA band patterns were detected with MFGp 0019 primer. Ten Korean ginseng cultivars shared the same size of amplified DNAs (389 bp), but 'Cheonryang' showed a different size. Thus 'Cheonryang' can be efficiently distinguished from the other ten ginseng cultivars by using the MFGp 0019 primer. In the case of MFGp 0248, two different sizes of DNA band patterns were detected in the eleven ginseng cultivars. Same sized amplified DNA bands (307 bp) were shown in five cultivars (Chunpoong, Gopoong, Kumpoong, Cheongsun, Sunhyang) and 254 bp sized DNA bands were identified in the other 6 cultivars (Yunpoong, Sunpoong, Sunun, Sunone, Cheonryang, K-1). In conclusion, the two STS primers, MFGp 0019, and MFGp 0248, provide a rapid and reliable method for the specific identification of 'Cheonryang' cultivar from a large number of samples.

• Journal of KIISE:Databases
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• v.34 no.2
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• pp.109-118
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• 2007

Recently, flash memory is used to store data in mobile computing devices such as PDAs, SmartCards, mobile phones and MP3 players. These devices need index structures like the B-tree to efficiently support some operations like insertion, deletion and search. The BFTL(B-tree Flash Translation Layer) technique was first introduced which is for implementing the B-tree on flash memory. Flash memory has characteristics that a write operation is more costly than a read operation and an overwrite operation is impossible. Therefore, the BFTL method focuses on minimizing the number of write operations resulting from building the B-tree. However, we indicate in this paper that there are many rooms of improving the performance of the I/O cost in building the B-tree using this method and it is not practical since it increases highly the usage of the SRAM memory storage. In this paper, we propose a BOF(the B-tree On Flash memory) approach for implementing the B-tree on flash memory efficiently. The core of this approach is to store index units belonging to the same B-tree node to the same sector on flash memory in case of the replacement of the buffer used to build the B-tree. In this paper, we show that our BOF technique outperforms the BFTL or other techniques.

• Journal of Internet Computing and Services
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• v.16 no.1
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• pp.75-82
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• 2015

Major corporations and portals have implemented a link server that connects Content Management Systems (CMS) to the physical address of content in a database (DB) to support efficient content use in web-based environments. In particular, a link server automatically connects the physical address of content in a DB to the content URL shown through a web browser screen, and re-connects the URL and the physical address when either is modified. In recent years, the number of users of digital content over the web has increased significantly because of the advent of the Big Data environment, which has also increased the number of link validity checks that should be performed in a CMS and a link server. If the link validity check is performed through an existing URL-based sequential method instead of petabyte or even etabyte environments, the identification rate of dead links decreases because of the degradation of validity check performance; moreover, frequent link checks add a large amount of workload to the DB. Hence, this study is aimed at providing a link server that can recognize URL link deletion or addition through analysis on the B-tree-based Information Identifier count per interval based on a large amount of URLs in order to resolve the existing problems. Through this study, the dead link check that is faster and adds lower loads than the existing method can be performed.