Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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A Rapid Identification of Korean Ginseng Cultivar, Cheonryang, using Specific DNA Markers

고려인삼 신품종 '천량' 특이적 DNA 판별 마커 개발

  • Jo, Ick Hyun (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Kim, Young Chang (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Kim, Jang Uk (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Lee, Seung Ho (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Lim, Ji Young (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Moon, Ji Young (Experiment Research Institute of National Agricultural Products Quality Management Service, MIFAFF) ;
  • Noh, Bong Soo (Department of Food Science and Technology, Seoul Women's University) ;
  • Hyun, Dong Yun (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Kim, Dong Hwi (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Kim, Kee Hong (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Bang, Kyong Hwan (Department of Herbal Crop Research, NIHHS, RDA)
  • 조익현 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 김영창 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 김장욱 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 이승호 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 임지영 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 문지영 (국립농산물품질관리원 시험연구소) ;
  • 노봉수 (서울여자대학교 식품공학과) ;
  • 현동윤 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 김동휘 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 김기홍 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 방경환 (농촌진흥청 국립원예특작과학원 인삼특작부)
  • Received : 2014.09.22
  • Accepted : 2014.10.30
  • Published : 2014.12.30
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Abstract

This study describes the efficient method for the discrimination of 'Cheonryang' in Panax ginseng Meyer using a STS primer. A total of 208 STS primers were applied to polymerase chain reaction (PCR) amplification for discriminating Korean ginseng cultivars. Co-dominant polymorphic band patterns were generated with two primers, MFGp 0019, MFGp 0248, and successful identification of 'Cheonryang' was achieved from out of 11 Korean ginseng cultivars. Two different sizes of DNA band patterns were detected with MFGp 0019 primer. Ten Korean ginseng cultivars shared the same size of amplified DNAs (389 bp), but 'Cheonryang' showed a different size. Thus 'Cheonryang' can be efficiently distinguished from the other ten ginseng cultivars by using the MFGp 0019 primer. In the case of MFGp 0248, two different sizes of DNA band patterns were detected in the eleven ginseng cultivars. Same sized amplified DNA bands (307 bp) were shown in five cultivars (Chunpoong, Gopoong, Kumpoong, Cheongsun, Sunhyang) and 254 bp sized DNA bands were identified in the other 6 cultivars (Yunpoong, Sunpoong, Sunun, Sunone, Cheonryang, K-1). In conclusion, the two STS primers, MFGp 0019, and MFGp 0248, provide a rapid and reliable method for the specific identification of 'Cheonryang' cultivar from a large number of samples.

Keywords

References

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