• Title/Summary/Keyword: Delay Tolerant

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Isolation of Surfactant-Resistant Pseudomonads from the Estuarine Surface Microlayer

  • Louvado, Antonio;Coelho, Francisco J.R.C.;Domingues, Patricia;Santos, Ana L.;Gomes, Newton C.M.;Almeida, Adelaide;Cunha, Angela
    • Journal of Microbiology and Biotechnology
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    • v.22 no.3
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    • pp.283-291
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    • 2012
  • Bioremediation efforts often rely on the application of surfactants to enhance hydrocarbon bioavailability. However, synthetic surfactants can sometimes be toxic to degrading microorganisms, thus reducing the clearance rate of the pollutant. Therefore, surfactant-resistant bacteria can be an important tool for bioremediation efforts of hydrophobic pollutants, circumventing the toxicity of synthetic surfactants that often delay microbial bioremediation of these contaminants. In this study, we screened a natural surfactant-rich compartment, the estuarine surface microlayer (SML), for cultivable surfactant-resistant bacteria using selective cultures of sodium dodecyl sulfate (SDS) and cetyl trimethylammonium bromide (CTAB). Resistance to surfactants was evaluated by colony counts in solid media amended with critical micelle concentrations (CMC) of either surfactants, in comparison with non-amended controls. Selective cultures for surfactant-resistant bacteria were prepared in mineral medium also containing CMC concentrations of either CTAB or SDS. The surfactantresistant isolates obtained were tested by PCR for the Pseudomonas genus marker gacA gene and for the naphthalene-dioxygenase-encoding gene ndo. Isolates were also screened for biosurfactant production by the atomized oil assay. A high proportion of culturable bacterioneuston was tolerant to CMC concentrations of SDS or CTAB. The gacA-targeted PCR revealed that 64% of the isolates were Pseudomonads. Biosurfactant production in solid medium was detected in 9.4% of tested isolates, all affiliated with genus Pseudomonas. This study shows that the SML is a potential source of surfactant-resistant and biosurfactant-producing bacteria in which Pseudomonads emerge as a relevant group.

WiCoin : Wireless LAN Sharing Using Block Chain Technology (와이코인 : 블록체인 기술을 이용한 무선랜 공유)

  • Kim, Woo-Seong;Ryu, Kyoung-Ho;Park, Yang-Jae
    • Journal of Digital Convergence
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    • v.17 no.1
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    • pp.195-201
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    • 2019
  • This paper proposes a blockchain system to share Wireless Local Area Network (WLAN) that recently suffers from mutual interference among increasing devices using unlicensed bands. Blockchain technology can induce cooperation from users by incentivizing them with cryptocurrency like shown in Bitcoin example. In this paper, we describe Blockchain based access mechanism in WLAN instead of conventional authentication based access. Here, users can access any WLAN access point by paying through smart contract while they also receive payment from others. In order to support real-time transaction, we apply proof-of-authority that is realized by Byzantine fault tolerant protocol instead of well-known proof-of-work that requires huge computing power and delay.

Evaluation of Cold Tolerance-Related Traits of Recombinant Inbred Lines in Rice (벼 재조합 자식계통의 내냉성 관련 형질 분석)

  • Jeong Eung Gi;Ahn Sang Nag;Yea Jong Doo;Baek Man Kee;Choi Hae Chune;Yi Gihwan;Nam Min-Hee;Yoon Kyung Min
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.50 no.3
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    • pp.205-211
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    • 2005
  • This study was carried out to construct cold-tolerance characteristics. The RILs were developed from progenies of a cross between cold-susceptible Tongil-type rice variety, Milyang 23 and cold-tolerant Japonica rice variety, Stejaree 45 by single seed descent methods. The 175 RILs $(F_8)$ were evaluated for cold tolerance traits by field screening under cold-water irrigation. Frequency distribution of RILs in leaf discoloration, heading delay, culm length reduction and number of spikelets reduction displayed nearly normal distributions with transgressive segregations to either side of parents, while the spikelet fertility reduction and panicle exsertion at low-temperature showed the more or less skewed continuous distribution toward the susceptible parent. Higher heritabilities over $60\%$ were observed in leaf discoloration, spikelet fertility reduction, panicle exsertion, while relatively lower heritabilities less than $40\%$ were observed in culm length reduction, number of spikelets reduction and grain yield reduction. Some cold-tolerance RILs were selected effectively by cold water irrigation, which are expected to be good materials in breeding program for cold tolerance.

A Hardware Barrier Synchronization using Multi -drop Scheme in Parallel Computer Systems (병렬 컴퓨터 시스템에서의 Multi-drop 방식을 사용한 하드웨어 장벽 동기화)

  • Lee, June-Bum;Kim, Sung-Chun
    • Journal of KIISE:Computer Systems and Theory
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    • v.27 no.5
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    • pp.485-495
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    • 2000
  • The parallel computer system that uses parallel program on the application such as a large scale business or complex operation is required. One of crucial operation of parallel computer system is synchronization. A representative method of synchronization is barrier synchronization. A barrier forces all process to wait until all the process reach the barrier and then releases all of the processes. There are software schemes, hardware scheme, or combinations of these mechanism to achieve barrier synchronization which tends to use hardware scheme. Besides, barrier synchronization lets parallel computer system fast because it has fewer start-up overhead. In this paper, we propose a new switch module that can implement fast and fault-tolerant barrier synchronization in hardware scheme. A proposed barrier synchronization is operated not in full-switch-driven method but in processor-driven method. An effective barrier synchronization is executed with inexpensive hardware supports. Therefore, a new proposed hardware barrier synchronization is designed that it is operated in arbitrary network topology. In this paper, we only show comparison of barrier synchronization on Multistage Interconnection Network. This research results in 24.6-24.8% reduced average delay. Through this result, we can expect lower average delay in irregular network.

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Meteorological Constraints and Countermeasures in Rice Breeding -Breeding for cold tolerance- (기상재해와 수도육종상의 대책 - 내냉성품종육성방안-)

  • Mun-Hue Heu;Young-Soo Han
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.27 no.4
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    • pp.371-384
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    • 1982
  • Highly cold tolerant varieties are requested not only at high latitute cool area but also tropical high elevated areas, and the required tolerance is different from location to location. IRRI identified 6 different types of cold tolerance required in the world for breeding purpose; a) Hokkaido type, b) Suweon type, c) Taipei 1st season type, d) Taipei 2nd season type, e) Tropical alpine type and, f) Bangladesh type. The cold tolerance requested in Korea is more eargent in Tongil group cultivars and their required tolerance is the one such as the physiological activities at low temperature are as active as in Japonica group cultivars at least during young seedling stage and reproduction stage. With conventional Japonica cultivars, such cold tolerant characters are requested as short growth duration but stable basic vegetative growth, less sensitive to high temperature and less prolonged growth duration at low temperature. The methods screening for cold tolerance were developed rapidly after the Tongil cultivar was reliesed. The facilities of screening for cold tolerance, such as, low temperature incubator, cold water tank, growth cabinet, phytotron, cold water nursery in Chuncheon, breeding nursery located in Jinbu, Unbong and Youngduk, are well established. Foreign facilities such as, cold water tank with the rapid generation advancement facilities, cold nurseries located in Banaue, Kathmandu and Kashimir may be available for the screening of some limitted breeding materials. For the reference, screening methods applied at different growth stages in Japan are introduced. The component characters of cold tolerance are not well identified, but the varietal differences in a) germinability, b) young seedling growth, c) rooting, d) tillering, e) discolation, f) nutrition uptake, g) photosynthesis rate, h) delay in heading, i) pollen sterility, and j) grain fertility at low temperature are reported to be distinguishable. Relationships among those traits are not consistent. Reported studies on the inheritance of cold tolerance are summarized. Four or more genes are controlling low temperature germinability, one or several genes are controlling seedling tolerance, and four or more genes are responsible for the pollen fertility of the rice treated with cold air or grown in the cold water nursery. But most of those data indicate that the results may come out in different way if those were tested at different temperature. Many cold tolerant parents among Japonicas, Indicas and Javanicas were identified as the results of the improvement of cold tolerance screening techniques and IRTP efforts and they are ready to be utilized. Considering a) diversification of germ plasm, b) integration of resistances to diseases and insects, c) identification of adaptability of recommending cultivars and, d) systematic control of recommending cultivars, breeding strategies for short term and long term are suggested. For short term, efforts will be concentrated mainly to the conventional cultivar group. Domestic cultivars will be used as foundation stock and ecologically different foreign introductions such as from Hokkaido, China or from Taiwan, will be used as cross parents for the adjustment of growth durations and synthsize the prototype of tolerances. While at the other side, extreme early waxy Japonicas will be crossed with the Indica parents which are identified for their resistances to the diseases and insects. Through the back corsses to waxy Japonicas, those Indica resistances will be transfered to the Japonicas and these will be utilized to the crosses for the improvement of resistances of prototype. For the long term, efforts will be payed to synthsize all the available tolerances identified any from Japonicas, Indicas and Javanicas to diversify the germ plasm. The tolerant cultivars newly synthsized, should be stable and affected minimum. to the low temperature at all the growing stages. The resistances to the diseases and insects should be integrated also. The rapid generation advancement, pollen culture and international cooperations were emphasized to maximize the breeding efficiency.

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Physiological and Ecological Studies on the Low Temperature Damages of Rice (Oryza sativia L.) (수도의 저온장해에 관한 생리 생태학적 연구)

  • 오윤진
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.26 no.1
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    • pp.1-31
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    • 1981
  • Experiments were conducted to investigate rice varietal response to low water and air temperatures at different growth stages from 1975 to 1980 in a phytotron in Suweon and in a cold water nursery in Chooncheon. Germination ability, seedling growth, sterility of laspikelets, panicle exertion, discoloration of leaves, and delay of heading of recently developed indica/japonica cross(I/J), japonica, and indica varieties at low air temperature or cold water were compared to those at normal temperature or natural conditions. The results are summarized as follows: 1. Practically acceptable germination rate of 70% was obtained in 10 days after initiation of germination test at 15\circ_C for japonica varieties, but 15 days for IxJ varieties. Varietal differences in germination ability at suboptimal temperature was greatest at 16\circ_C for 6 days. 2. Cold injury of rice seedlings was most severe at the 3.0-and 3.5-leaf stage and it was reduced as growth stage advanced. A significant positive correlation was observed between cold injury at 3-leaf stage and 6-leaf stage. 3. At day/night temperatures of 15/10\circ_C seedlings of both japonica and I/J varieties were dead in 42 days. At 20/15\circ_C japonica varieties produced tillers actively, but tillering of I/J varieties was retarded a little. At 25/15\circ_C, both japonica and I/J varieties produced tillers most actively. Increase in plant height was proportional to the increase in all varieties. 4. In I/J varieties the number of differentiated panicle rachis branches and spikelets was reduced at a day-night temperature of 20-15\circ_C compared to 25-20 or 30-25\circ_C, but not in japonica varieties although panicle exertion was retarded at 20-15\circ_C. The number of spikelets was not correlated with the number of primary rachis branches, but positively correlated with that of secondary rachis branches. 5. Heading of rice varieties treated with 15\circ_C air temperature at meiotic stage was delayed compared to that at tillering stage by 1-3 days and heading was delayed as duration of low temperature treatment increased. 6. At cold water treatment of 17\circ_C from tillering to heading stage, heading of japonica, I/J, and cold tolerant indica varieties was delayed 2-6, 3-9, and 4-5 days, respectively, Growth stage sensitive to delay of heading delay at water treatment were tillering stage, meiotic stage, and booting tage in that order, delay of heading was greater in indica corssed japonica(Suweon 264), japonica(Suweon 235), and cold tolerant indica(Lengkwang) varieties in that order. Delay of heading due to cold water treatment was positively correlated with culm length reduction and spikelet sterility. 7. Elongation of culms and exertion of panicles of rice varieties treated with low air temperature 17\circ_C. Culm length reduction rate of tall varieties was lower than that of short statured varieties at low temperature. Panicle exertion was most severaly retarded with low temperature treatment at heading stage. Generally, retardation of panicle exertion of 1/1 varieties was more severe than that of japonica varieties at low temperature. There was a positive correlation between panicle exertion and culm length at low temperature. 8. The number of panicles was increased with cold water treatment at tillering stage, but reduced at meiotic stage. As time of cold water treatment was conducted at earlier growth stage, culm length was shorter and panicle exertion poorer. 9. Sterility of all rice varieties was negligible at 17\circ_C for three days but 30.3-85.2% of strility was observed for nine-day treatment at 17\circ_C. Among the tested varieties, sterility of Suweon 264 and Milyang 42 was highest and that of Suweon 290 and Suweon 287 was lowest. The most sensitive growth stage to low temperature induced sterility was from 15 to 5 days before heading. There was positive correlation between sterility of rice plants treated with low temperature at meiotic and heading stage. 10. Percentage of spikelet sterility was greatest at cold water treatment at meiotic stage (auricle distance -15~-10cm) and it was higher in 1/1 (Suweon 264, Joseng tongil), japonica (Nongbaek, Towada), and cold tolerance indica(Lengkwang) varieties in the order. Level of cold water and position of young-ear affected on the sterility of varieties at meiotic stage; percentage of spikelet sterility of variety, Lengkwang, of which young-ear was located above the cold water level was high, but that of short statured variety, Suweon 264, of which young-ear was located in the cold water was lower. 11. Percentage of ripened grains was not reducted at 15\circ_C air temperature for three days at full heading stage in all varieties. However, at six-day low temperature treatment Suweon 287, Suweon 264 showed percentage of ripended grains lower than 60%, but at nine-day low temperature treatment all varieties showed percentage of ripened grains lower than 60%. Low temperature treatment of 17\circ_C from 10 days after heading for 20 days did not affect on the ripening of all varieties. 12. Uptake of nitrogen, phosphorous, potassium, calcium, and magnesium in whole plants was higher at average air temperature of 25\circ_C, but concentration of the elements was lower compared to those at 19\circ_C. However, both total uptake and concentration of manganese were higher at 19\circ_C compared to 25\circ_C. 13. Higher application of nitrogen, phosphorus, silicate, and compost increased yield of rice due to increased number of panicles and spike let fertility in cold water irrigated paddy.

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Spring Phonology of a Grapevine Cultivar under the Changing Climate in Korea during 1921-2000 (겨울기온 상승에 따른 낙엽과수의 휴면생태 변화)

  • Jung Jea-Eun;Seo Hee-Cheol;Chung U-Ran;Yun Jin-I.
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.8 no.2
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    • pp.116-124
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    • 2006
  • Remarkable winter season warming has been observed in East Asian countries during the last century. Accordingly, significant effects on dormancy and the resulting budburst of deciduous trees are expected. However phenological observations are rare and insufficient compared with the long-time climate records in the same region. A chill-day accumulation, which can be estimated from daily maximum and minimum temperature, is expected to make a reasonable proxy for dormancy depth of temperate zone fruit trees. To simulate dormancy depth during 1921-2004, a chill-day model parameterized for 'Campbell Early' grapevine, which is the major cultivar grown virtually anywhere in South Korea, was applied to daily temperature data at 8 locations in South Korea. The calculations showed that the chilling requirement for breaking endo-dormancy of this grapevine cultivar can be satisfied by mid-January to late February in South Korea, and the date was delayed going either northward or southward from the 'Daegu-Jeonju' line crossing the middle of South Korea in the east-west direction. Maximum length of the cold tolerant period (the number of days between endo-dormancy release and forced dormancy release) showed the same spatial pattern. When we divide the 83 years into 3 periods (I: 1921-1950, II: 1951-1980, and III: 1981-2004) and get the average of each period, dormancy release date of period III was accelerated by as much as 15 days compared with that of period I at all locations except Jeju (located in the southernmost island with subtropical climate) where an average15-day delay was predicted. The cold- tolerant period was also shortened at 6 out of 8 locations. As a result, budburst of 'Campbell Early' in spring was accelerated by 6 to 10 days at most locations, while inter-annual variation in budburst dates was increased at all locations. The earlier budburst after the 1970s was due to (1) warming in winter resulting in earlier dormancy release (Incheon, Mokpo, Gangneung, and Jeonju), (2) warming in early spring accelerating regrowth after breaking dormancy (Busan and Jeju), and (3) both of them (Seoul and Daegu).

Automatic On-Chip Glitch-Free Backup Clock Changing Method for MCU Clock Failure Protection in Unsafe I/O Pin Noisy Environment (안전하지 않은 I/O핀 노이즈 환경에서 MCU 클럭 보호를 위한 자동 온칩 글리치 프리 백업 클럭 변환 기법)

  • An, Joonghyun;Youn, Jiae;Cho, Jeonghun;Park, Daejin
    • Journal of the Institute of Electronics and Information Engineers
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    • v.52 no.12
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    • pp.99-108
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    • 2015
  • The embedded microcontroller which is operated by the logic gates synchronized on the clock pulse, is gradually used as main controller of mission-critical systems. Severe electrical situations such as high voltage/frequency surge may cause malfunctioning of the clock source. The tolerant system operation is required against the various external electric noise and means the robust design technique is becoming more important issue in system clock failure problems. In this paper, we propose on-chip backup clock change architecture for the automatic clock failure detection. For the this, we adopt the edge detector, noise canceller logic and glitch-free clock changer circuit. The implemented edge detector unit detects the abnormal low-frequency of the clock source and the delay chain circuit of the clock pulse by the noise canceller can cancel out the glitch clock. The externally invalid clock source by detecting the emergency status will be switched to back-up clock source by glitch-free clock changer circuit. The proposed circuits are evaluated by Verilog simulation and the fabricated IC is validated by using test equipment electrical field radiation noise

Energy-Efficient Data-Aware Routing Protocol for Wireless Sensor Networks (무선 센서 네트워크를 위한 에너지 효율적인 데이터 인지 라우팅 프로토콜)

  • Lee, Sung-Hyup;Kum, Dong-Won;Lee, Kang-Won;Cho, You-Ze
    • Journal of the Institute of Electronics Engineers of Korea CI
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    • v.45 no.6
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    • pp.122-130
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    • 2008
  • In many applications of wireless sensor networks, sensed data can be classified either normal or urgent data according to its time criticalness. Normal data such as periodic monitoring is loss and delay tolerant, but urgent data such as fire alarm is time critical and should be transferred to a sink with reliable. In this paper, by exploiting these data characteristics, we propose a novel energy-efficient data-aware routing protocol for wireless sensor networks, which provides a high reliability for urgent data and energy efficiency for normal data. In the proposed scheme, in order to enhance network survivability and reliability for urgent data, each sensor node forwards only urgent data when its residual battery level is below than a threshold. Also, the proposed scheme uses different data delivery mechanisms depending on the data type. The normal data is delivered to the sink using a single-path-based data forwarding mechanism to improve the energy-efficiency. Meanwhile, the urgent data is transmitted to the sink using a directional flooding mechanism to guarantee high reliability. Simulation results demonstrate that the proposed scheme could significantly improve the network lifetime, along with high reliability for urgent data delivery.

The Cell Resequencing Buffer for the Cell Sequence Integrity Guarantee for the Cyclic Banyan Network (사이클릭 벤얀 망의 셀 순서 무결성 보장을 위한 셀 재배열 버퍼)

  • 박재현
    • Journal of the Institute of Electronics Engineers of Korea TC
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    • v.41 no.9
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    • pp.73-80
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    • 2004
  • In this paper, we present the cell resequencing buffer to solve the cell sequence integrity problem of the Cyclic banyan network that is a high-performance fault-tolerant cell switch. By offering multiple paths between input ports and output ports, using the deflection self-routing, the Cyclic banyan switch offer high reliability, and it also solves congestion problem for the internal links of the switch. By the way, these multiple paths can be different lengths for each other. Therefore, the cells departing from an identical source port and arriving at an identical destination port can reach to the output port as the order that is different from the order arriving at input port. The proposed cell resequencing buffer is a hardware sliding window mechanism. to solve such cell sequence integrity problem. To calculate the size of sliding window that cause the prime cost of the presented device, we analyzed the distribution of the cell delay through the simulation analyses under traffic load that have a nonuniform address distribution that express tile Property of traffic of the Internet. Through these analyses, we found out that we can make a cell resequencing buffer by which the cell sequence integrity is to be secured, by using a, few of ordinary memory and control logic. The cell resequencing buffer presented in this paper can be used for other multiple paths switching networks.