• Journal of the Korean Society of Radiology
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• v.12 no.4
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• pp.497-503
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• 2018

The purpose of this study was to investigate the degree of image degradation and the improvement of image quality caused by the density difference between the orthodontic filling material and the surrounding anatomical structure during the examination of the facial CT by quantitative and qualitative analysis. The teeth were scanned using 64-MDCT (Discovery 750 HD, GE HEALTH CARE, Milwaukee, USA). The teeth were scanned and compared according to tube voltage, silicone application, and MAR application. As a result, 10.36% CT value decreased at 140 kVp and 5.81% decrease at the application of silicon material. As a result of the qualitative evaluation, it was evaluated that 7 of the 10 observers and 3 of the acceptors were applied to the MAR algorithm. Therefore, it is possible to reduce the unnecessary burden on the radiation exposure dose as well as to reduce the loss of image data by reducing the high density artifacts, as well as the inspection parameters used in the current clinical application and various algorithms that can reduce the high density artifacts. It can be expected to provide a lot of image information.

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.27 no.4
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• pp.246-257
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• 2015

The progressive degradation paths of structures have quantitatively been tracked by using stochastic processes, such as Wiener process, gamma process and compound Poisson process, in order to consider both the sampling uncertainty due to the usual lack of damage data and the temporal uncertainty associated with the deterioration evolution. Several important features of stochastic processes which should carefully be considered in application of the stochastic processes to practical problems have been figured out through assessing cumulative damage and lifetime distribution as a function of time. Especially, the Wiener process and the gamma process have straightforwardly been applied to armors of rubble-mound breakwaters by the aid of a sample path method based on Melby's formula which can estimate cumulative damage levels of armors over time. The sample path method have been developed to calibrate the related-parameters required in the stochastic modelling of armors of rubble-mound breakwaters. From the analyses, it is found that cumulative damage levels of armors have surely been saturated with time. Also, the exponent of power law in time, that plays a significant role in predicting the cumulative damage levels over time, can easily be determined, which makes the stochastic models possible to track the cumulative damage levels of armors of rubble-mound breakwaters over time. Finally, failure probabilities with respect to various critical limits have been analyzed throughout its anticipated service life.

• Journal of Korean Society of Environmental Engineers
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• v.27 no.11
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• pp.1186-1192
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• 2005

The relationship between bacteria and anaerobic archaea, sludge yield coefficient and nitrogen removal rate were investigated in intermittent aeration systems(I/A) with added archaea, I/A and conventional activated sludge system. As the archaea solution was added to the I/A reactor, organic removal rate as well as nitrogen removal rate increased. Also, sludge production rate in I/A system added the archaea was maintained lower than other systems because sludge yield coefficient was decreased due to the role of anaerobic archaea such as anaerobic degradation of organics. The experimental data supported the possibility of symbiotic activated sludge system with anaerobic archaea under intermittent aeration, leading to the enhanced nitrogen removal. Crucial results to be presented are: 1) specific oxygen utilization rate(SOUR) of the I/A-arch system was $2.9\;mg-O_2/(g-VSS{\cdot}min)$. SOUR and nitrification rate of the sludge from the I/A-arch system was higher than those from the I/A and A/S reactors. 2) Removal efficiencies of $TCOD_{Cr}$ in the I/A-arch, I/A and A/S reactors were 93, 90 and 87%, respectively. 3) Nitrification occurred successfully in each reactor, while denitrification rate was much higher in the I/A-arch reactor. Efficiencies of TN removal in the I/A-arch, I/A and A/S reactors were 75, 63 and 33%, respectively.

• Journal of Conservation Science
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• v.32 no.3
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• pp.385-394
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• 2016

A fabric excavated from tombs or passed down is not easy to find its original color as it degrades and discolors by UV and visible rays, oxygen and microorganisms. LC-MS analysis is commonly used for separating and analyzing colors, but color extraction process is complicated and important in dye-qualitative analysis. To extract red colors from a fabric which is dyed with safflower and lac, solvents; hydrogen chloride, pyridine and oxalic acid are used and oxalic acid was the most effective solvent. Meanwhile, dyed samples were put in degradation condition; UV-A for 168 hours and analyzed with LC-MS to find out its colors'chemical changes. As a result, carthamin is detected in $T_R$ 13 min and laccaic acid A is detected in $T_R$ 10 min. However carthamin is not detected in a degraded fabric dying with safflower, it could be identified as a safflower fabric by the molecular weight of m/z 931. Through this study the most optimal method for red color extraction is found so it is expected to be used as a base line data for red color LC-MS analysis.

• Journal of Conservation Science
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• v.32 no.2
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• pp.203-213
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• 2016

Conservation treatment of modern cultural heritage rickshaw from National Museum of Korean Contemporary History were carried out. The Rickshaw is divided into three parts which are chair part, sun-block cover part, wheels part. Treatment was referred to analysis results of P-XRF, species identification, FT-IR and microscope observation on Rickshaw. Outer films of rickshaw were chipped off. Therefore, conservation and restoration treatment were carried out in the damaged area. Degradation in armrest, saddle and backside of chair may cause serious problem. Therefore, the reinforcement were carried out with similar materials. Dry and wet cleaning were performed in sun-block cover to remove white stain and corrosion contaminant. Furthermore, reinforcement and restoration were performed in damaged area. Rickshaw from National Museum of Korean Contemporary History was made with various materials. This research result expects to be a great example of conservation treatment for modern cultural heritage which is made of various materials and to be used as useful data for conservation and restoration in modern cultural heritage.

• Korean Journal of Animal Reproduction
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• v.27 no.4
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• pp.325-331
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• 2003

This study was performed to test the cellulose digestibility using the transgenic pigs harboring cellulose degradation gene D (CelD). After delivered offsprings between normal pig and transgenic swine, DNA was isolated from piglets tail for PCR analysis. In first generation, five out of 65 piglets showed CelD positive. Unfortunately, four CelD-positive pigs were died during growing, but one survived pig was used as a transgenic founder to produce F$_1$ descendents. Among 3 F$_1$ transgenic pigs produced, one died and the remaining two pigs were used to test the fiber digest efficiency. An assorted feed was composite of 5％ fiber with other ingredients. The feed of 3 kg per day was provided to the pigs including transgenic founders and littermate controls. The manure quantity was measured daily for a month, and all manures were dried for three days to analysis nitrogen, phosphate and fiber concentrations. The fiber digestion efficiencies of the transgenic F$_1$ pigs showed approximately 10％ higher than those of control pigs. Fiber digestion was not greatly improved in transgenic pigs as it had been expected approximately 30％. Nitrogen concentration of transgenic pig's manure was slowly decreased compare to the control pigs. Because there were only two transgenic pigs tested, a large number of transgenic pigs may be necessary to obtain more reliable data. Breeding of animals to obtain sufficient transgenic pigs subjected for a further study is on progress. Taken together, this study demonstrated successful production of transgenic pigs with increase of cellulose digestibility in the porcine feed.

• Journal of Korean Tunnelling and Underground Space Association
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• v.20 no.2
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• pp.513-525
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• 2018

A 3 dimensional numerical analysis for shield TBM tunnel should take into account various characteristics of the shield TBM excavation, such as gap, tail void, segment installation, and backfill injection. However, analysis method considering excavation characteristics are generally mixed with various method, resulting in concern of consistency and reliability degradation of the analytical results. In this paper, a parametric study is carried out by using actually measured ground settlement data on various methods that can be used for 3 dimensional numerical analysis of shield TBM tunneling. As a result, we have analyzed and arranged an analytical method to predict similarly the behavior of ground settlement and tunnel face pressure at the design stage. Skin plate pressure, backfill pressure and soil model have been identified as the most significant influences on the ground settlement. The grout pressure model is considered to be applicable when there is no volume loss information on the excavated ground, such as seabed tunnels, or when it is important to identify the behavior around a tunnel, such as surface settlement as well as face pressure. And it is considered that designers can use these guidelines as a base material to perform a reasonable 3 dimensional numerical analysis that reflects the ground conditions and the features of the shield TBM tunneling.

• Asian-Australasian Journal of Animal Sciences
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• v.14 no.6
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• pp.880-884
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• 2001

Rumen microbiology research has undergone several evolutionary steps: the isolation and nutritional characterization of readily cultivated microbes; followed by the cloning and sequence analysis of individual genes relevant to key digestive processes; through to the use of small subunit ribosomal RNA (SSU rRNA) sequences for a cultivation-independent examination of microbial diversity. Our knowledge of rumen microbiology has expanded as a result, but the translation of this information into productive alterations of ruminal function has been rather limited. For instance, the cloning and characterization of cellulase genes in Escherichia coli has yielded some valuable information about this complex enzyme system in ruminal bacteria. SSU rRNA analyses have also confirmed that a considerable amount of the microbial diversity in the rumen is not represented in existing culture collections. However, we still have little idea of whether the key, and potentially rate-limiting, gene products and (or) microbial interactions have been identified. Technologies allowing high throughput nucleotide and protein sequence analysis have led to the emergence of two new fields of investigation, genomics and proteomics. Both disciplines can be further subdivided into functional and comparative lines of investigation. The massive accumulation of microbial DNA and protein sequence data, including complete genome sequences, is revolutionizing the way we examine microbial physiology and diversity. We describe here some examples of our use of genomics- and proteomics-based methods, to analyze the cellulase system of Ruminococcus flavefaciens FD-1 and explore the genome of Ruminococcus albus 8. At Illinois, we are using bacterial artificial chromosome (BAC) vectors to create libraries containing large (>75 kbases), contiguous segments of DNA from R. flavefaciens FD-1. Considering that every bacterium is not a candidate for whole genome sequencing, BAC libraries offer an attractive, alternative method to perform physical and functional analyses of a bacterium's genome. Our first plan is to use these BAC clones to determine whether or not cellulases and accessory genes in R. flavefaciens exist in clusters of orthologous genes (COGs). Proteomics is also being used to complement the BAC library/DNA sequencing approach. Proteins differentially expressed in response to carbon source are being identified by 2-D SDS-PAGE, followed by in-gel-digests and peptide mass mapping by MALDI-TOF Mass Spectrometry, as well as peptide sequencing by Edman degradation. At Ohio State, we have used a combination of functional proteomics, mutational analysis and differential display RT-PCR to obtain evidence suggesting that in addition to a cellulosome-like mechanism, R. albus 8 possesses other mechanisms for adhesion to plant surfaces. Genome walking on either side of these differentially expressed transcripts has also resulted in two interesting observations: i) a relatively large number of genes with no matches in the current databases and; ii) the identification of genes with a high level of sequence identity to those identified, until now, in the archaebacteria. Genomics and proteomics will also accelerate our understanding of microbial interactions, and allow a greater degree of in situ analyses in the future. The challenge is to utilize genomics and proteomics to improve our fundamental understanding of microbial physiology, diversity and ecology, and overcome constraints to ruminal function.

• Journal of Microbiology and Biotechnology
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• v.25 no.9
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• pp.1433-1441
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• 2015

Pollution resulting from the discharge of textile dyes into water systems has become a major global concern. Because peroxidases are known for their ability to decolorize and detoxify textile dyes, the peroxidase activity of Vitreoscilla hemoglobin (VHb) has recently been studied. It is found that VHb and variants of this enzyme show great promise for enzymatic decolorization of dyes and may play a role in achieving their successful removal from industrial wastewater. The level of VHb peroxidase activity correlates with two amino acid residues present within the conserved distal pocket, at positions 53 and 54. In this work, sitedirected mutagenesis of these residues was performed and resulted in improved VHb peroxidase activity. The double mutant, Q53H/P54C, shows the highest dye decolorization and removal efficiency, with 70% removal efficiency within 5 min. UV spectral studies of Q53H/P54C reveals a more compact structure and an altered porphyrin environment (λ_Soret = 413 nm) relative to that of wild-type VHb (λ_Soret = 406), and differential scanning calorimetry data indicate that the VHb variant protein structure is more stable. In addition, circular dichroism spectroscopic studies indicate that this variant's increased protein structural stability is due to an increase in helical structure, as deduced from the melting temperature, which is higher than 90℃. Therefore, the VHb variant Q53H/P54C shows promise as an excellent peroxidase, with excellent dye decolorization activity and a more stable structure than wild-type VHb under high-temperature conditions.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.44 no.11
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• pp.949-956
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• 2016

There are a number of effects by solar radiation in many aerospace industrial fields, such as degradation of mechanical properties, sealing effect of sealants or decolorization. Because it takes long time to investigate these effects by using the light of natural state, new methods are developed for accelerating this phenomenon. In this paper, we developed an apparatus to simulate accelerated solar radiation phenomenon selecting irradiation intensity $1,120W/m^2$ as the designed environment. Epoxy polymer as the composite material was chosen and processed by ASTM-D638, a reference for tensile test of polymer and plastic. Total color shift was selected as the test category to evaluate acceleration of the test. We obtained acceleration factors and numerical model from test data and concluded it can shorten test periods by accelerated irradiation intensity of $1,120W/m^2$.