• The Plant Pathology Journal
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• 제39권5호
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• pp.449-465
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• 2023

Plants are challenged by various pathogens throughout their lives, such as bacteria, viruses, fungi, and insects; consequently, they have evolved several defense mechanisms. In addition, plants have developed localized and systematic immune responses due to biotic and abiotic stress exposure. Animals are known to activate DNA damage responses (DDRs) and DNA damage sensor immune signals in response to stress, and the process is well studied in animal systems. However, the links between stress perception and immune response through DDRs remain largely unknown in plants. To determine whether DDRs induce plant resistance to pathogens, Arabidopsis plants were treated with bleomycin, a DNA damage-inducing agent, and the replication levels of viral pathogens and growth of bacterial pathogens were determined. We observed that DDR-mediated resistance was specifically activated against viral pathogens, including turnip crinkle virus (TCV). DDR increased the expression level of pathogenesis-related (PR) genes and the total salicylic acid (SA) content and promoted mitogen-activated protein kinase signaling cascades, including the WRKY signaling pathway in Arabidopsis. Transcriptome analysis further revealed that defense-and SA-related genes were upregulated by DDR. The atm-2atr-2 double mutants were susceptible to TCV, indicating that the main DDR signaling pathway sensors play an important role in plant immune responses. In conclusion, DDRs activated basal immune responses to viral pathogens.

• Journal of Dairy Science and Biotechnology
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• 제42권2호
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• pp.35-47
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• 2024

Milk exosomes contain several bioactive molecules, including lipids, proteins, and miRNAs, which enhance immune response. This study aimed to assess the resistance effects of bovine colostrum exosomes (BCEs) on pathogenic microbial infections in a Caenorhabditis elegans model. BCEs have been shown to enhance the protective response of C. elegans to pathogenic bacterial infections. Our study revealed that BCE extended the lifespan of worms compared to control OP50 worms. In addition, nematode colostrum exosomes promoted nematode resistance to four pathogenic bacteria and prolonged their lifespan in a killing assay. In contrast, mature milk-derived exosomes (BME) did not affect the resistance and lifespan of nematodes exposed to pathogenic bacteria. BCE exposure extended the lifespan of C. elegans against pathogenic infections by stimulating the innate immune response and increasing antimicrobial protein expression. Using biological process-related gene ontology (GO) enrichment analysis, the significantly upregulated GO terms related to C. elegans immunity in BCE-exposed C. elegans included defense, innate immunity, and immune responses. This study demonstrated that BCE enhanced the host defense of C. elegans to prolong its lifespan, thereby suggesting a new natural product against infection by pathogenic bacteria.

• 한국추진공학회지
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• 제19권1호
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• pp.68-75
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• 2015

본 논문에서는 고체추진기관 밸브의 내부에 장착한 핀틀의 구동 응답, 추력 및 압력 데이터를 이용하여, 연소시험 시 발생한 고체추진기관 내부 압력의 비정상 특성을 분석하였다. 고체추진기관 밸브의 내부에 핀틀이라는 구조체를 장착하여 핀틀의 축방향 구동을 통해서 노즐목 면적을 조절하고, 이를 통해 고체추진기관의 압력 및 추력을 실시간으로 제어할 수 있다. 이때 연소관 내부의 압력에 비정상 특성이 나타날 수 있으며, 이러한 비정상 특성은 다양한 원인이 종합적으로 영향을 미친 결과이다. 이 경우 핀틀의 구동 응답을 이용한 내부 압력의 재예측 및 추력 대 압력 비 분석을 통해서 핀틀 구동 응답 오차와 중공 튜브 삭마에 의한 노즐목 면적 변화라는 두 가지 큰 비정상 특성의 원인을 찾아내고, 각 원인들이 연소관 내부 압력에 미치는 영향을 개별적으로 분석하였다.

• Journal of Ginseng Research
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• 제47권6호
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• pp.773-783
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• 2023

Background: Gray mold, caused by Botrytis cinerea, is one of the major fungal diseases in agriculture. Biological methods are preferred over chemical fungicides to control gray mold since they are less toxic to the environment and could induce the resistance to pathogens in plants. In this work, we try to understand if ginseng defense to B. cinerea could be induced by fungal hypovirulent strain △BcSpd1. BcSpd1 encodes Zn(II)₂Cys₆ transcription factor which regulates fungal pathogenicity and we recently reported △BcSpd1 mutants reduced fungal virulence. Methods: We performed transcriptomic analysis of the host to investigate the induced defense response of ginseng treated by B. cinerea △BcSpd1. The metabolites in ginseng flavonoids pathway were determined by UPLC-ESI-MS/MS and the antifungal activates were then performed. Results: We found that △BcSpd1 enhanced the ginseng defense response when applied to healthy ginseng leaves and further changed the metabolism of flavonoids. Compared with untreated plants, the application of △BcSpd1 on ginseng leaves significantly increased the accumulation of p-coumaric acid and myricetin, which could inhibit the fungal growth. Conclusion: B. cinerea △BcSpd1 could effectively induce the medicinal plant defense and is referred to as the biological control agent in ginseng disease management.

• 한국항행학회논문지
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• 제6권3호
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• pp.211-222
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• 2002

본 논문은 탄도미사일 방어의 위성센서 효용성에 대해 추정기법을 이용하여 설명한다. 탄도미사일은 고유 비행특성상 대응시간과 대응수단을 극히 제한시킨다. 이러한 점에서 조기경보 및 감시위성은 중요한 의미를 지닌다. 미사일방어 체계의 DSP 위성과 SBIRS에 의한 미사일 발사위치 불확실성(LPU)과 중간 비행 단계의 위치불확실성(MLU)을 확장칼만필터를 사용한 추정이론에 의해 분석하고, 비회전형 DSP에 의한 상태 추정값의 불확실성을 회전형과 비교하여 정량적으로 제시했다.

• 융합보안논문지
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• 제20권4호
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• pp.91-101
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• 2020

과거 해외에서 발생한 APT 공격사례를 사이버 킬체인 모델과 TTP 모델로 분석하였다. 분석 결과 사이버 킬체인 모델은 전체적인 윤곽을 파악하는데 효과적이지만 구체적인 방어 전략을 수립하는 데에는 부적합하며, TTP 모델로 분석해야만 실질적인 방어 체제를 구비하는데 적합함을 알 수 있었다. 이러한 분석 결과를 바탕으로 사이버 공격을 대비하는 관점에서 심층 방어선 구축에 적합한 TTP 모델 관점에서 방어 기술 개발이 필요함을 제시한다.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.64.1-64
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• 2003

Inoculation of primary pepper leaves with an avirulent strain of Xanthomonas campestris pv. vesicatoria induced systemic acquired resistance (SAR) in secondary leaves. This SAR response was accompanied by the systemic expression of defense-related genes, a systemic microoxidative burst generating H2O2, and the systemic induction of ion-leakage and callose deposition in the non-inoculated, secondary leaves. Some defense-related genes encoding PR-1, chitinase, peroxidase, PR10, thionin, defensin and zinc-finger protein were distiilctly induced in the systemic leaves. The systemically striking accumulation of H$_2$O$_2$and strong increase in peroxidase activity in pepper was suggested to contribute to the triggering of cell death In the systemic micro-HRs, leading to the induction of SAR. Treatment of non-inoculated, secondary leaves with diphenylene iodinium (DPI), an inhibitor of the oxidative burst, substantially reduced the induction of some defense-related genes and subsequently SAR.

• The Plant Pathology Journal
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• 제15권1호
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• pp.8-13
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• 1999

Hypersensitive cell death of plants during incompatible plant-pathogen interactions is one of the efficient defense mechanisms of plants against pathogen infections. For better understanding of the molecular mechanisms involved in the plant hypersensitive response (HR), TMV-induced biotic plant cell death and CuSO4-induced abiotic plant cell death were compared in terms of expression patterns of ten different defense-related genes as molecular markers. The genes include five pathogenesis-related protein genes, two plant secondary metabolite-associated genes, two oxidative stress-related genes and one wound-inducible gene isolated from tobacco. Northern blot analyses revealed that a same set of defense-related genes was induced during both biotic and abiotic cell death but with different time and magnitude. The expression of defense-related genes in tobacco plants was temporarily coincided with the time of cell death. However, when suspension cell cultures was used to monitor the expression of defense-related genes, different patterns of the gene expression were detected. This result implies that three are common and, in addition, also different branches of signaling pathways leading to the induced expression of defense-related genes in tobacco during the pathogen- and heavy metal-induced cell death.

• The Plant Pathology Journal
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• 제25권4호
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• pp.389-399
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• 2009

A group of beneficial plant bacteria has been shown to increase crop growth referring to as plant growth-promoting rhizobacteria (PGPR). PGPR can decrease plant disease directly, through the production of antagonistic compounds, and indirectly, through the elicitation of a plant defense response termed induced systemic resistance (ISR). While the mechanism of PGPR-elicited ISR has been studied extensively in the model plant Arabidopsis, it is less well characterized in crop plants such as pepper. In an effort to better understand the mechanism of ISR in crop plants, we investigated the induction of ISR by Bacillus cereus strain BS107 against Xanthomonas axonopodis pv. vesicatoria in pepper leaves. We focused on the priming effect of B. cereus strain BS107 on plant defense genes as an ISR mechanism. Of ten known pepper defense genes that were previously reported to be involved in pathogen defense signaling, the expression of Capsicum annum pathogenesis-protein 4 and CaPR1 was systemically primed by the application of strain BS107 onto pepper roots confirming by quantitative-reverse transcriptase PCR. Our results provide novel genetic evidence of the priming effect of a rhizobacterium on the expression of pepper defense genes involved in ISR.

• 정보보호학회논문지
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• 제30권5호
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• pp.805-821
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• 2020

사이버 방어 훈련은 최신 사이버공격을 고려한 훈련을 수행해야 할 뿐만 아니라 사이버공격 대응 과정이 실전과 유사해야 그 효과가 크다고 할 수 있다. 또한 최근에는 사이버공격에 관계없이 정상업무를 수행하거나 그에 준하는 업무를 지원할 수 있는 사이버 회복력에 대한 훈련 역시 중요하다. 이에 본 논문에서는 국내에서 사이버공격 발생 시 발령되는 사이버위기 경보를 기반으로 실시간 사이버훈련을 수행함으로 사이버 회복력의 요소들을 강화시킬 수 있는 사이버 방어 훈련장을 제안한다. 사이버위기 경보 수준에 따라 관심·주의·경계·심각 경보 발령 시, 사이버 방어 훈련장은 해당 경보에 따라, 보안 요소를 점검하는 예방보안, 실시간으로 사이버공격 방어를 수행하는 실시간대응, 피해 시스템의 사고조사를 수행하는 사후대응 훈련을 수행할 수 있는 환경을 지원한다. 본 논문에서 제안하는 사이버 방어 훈련장은 사이버위기 환경에서 국내 실정에 맞는 사이버 방어 훈련을 수행함으로써 훈련생들이 사이버위협에 대한 실질적인 대응 역량을 제고 할 수 있다.