Journal of Ginseng Research

  • 제47권6호
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  • Pages.773-783
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  • 2023
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  • 1226-8453(pISSN)
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  • 2093-4947(eISSN)
고려인삼학회 (The Korean Society of Ginseng)
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Botrytis cinerea hypovirulent strain △BcSpd1 induced Panax ginseng defense

  • Shuhan Zhang (Laboratory of Tea and Medicinal Plant Pathology, Jilin University) ;
  • Junyou Han (Laboratory of Tea and Medicinal Plant Pathology, Jilin University) ;
  • Ning Liu (Institute of Special Animal and Plant Science, Chinese Academy of Agricultural Sciences) ;
  • Jingyuan Sun (Laboratory of Tea and Medicinal Plant Pathology, Jilin University) ;
  • Huchen Chen (Laboratory of Tea and Medicinal Plant Pathology, Jilin University) ;
  • Jinglin Xia (Laboratory of Tea and Medicinal Plant Pathology, Jilin University) ;
  • Huiyan Ju (Laboratory of Tea and Medicinal Plant Pathology, Jilin University) ;
  • Shouan Liu (Laboratory of Tea and Medicinal Plant Pathology, Jilin University)
  • 투고 : 2022.12.27
  • 심사 : 2023.08.30
  • 발행 : 2023.11.01
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초록

Background: Gray mold, caused by Botrytis cinerea, is one of the major fungal diseases in agriculture. Biological methods are preferred over chemical fungicides to control gray mold since they are less toxic to the environment and could induce the resistance to pathogens in plants. In this work, we try to understand if ginseng defense to B. cinerea could be induced by fungal hypovirulent strain △BcSpd1. BcSpd1 encodes Zn(II)2Cys6 transcription factor which regulates fungal pathogenicity and we recently reported △BcSpd1 mutants reduced fungal virulence. Methods: We performed transcriptomic analysis of the host to investigate the induced defense response of ginseng treated by B. cinerea △BcSpd1. The metabolites in ginseng flavonoids pathway were determined by UPLC-ESI-MS/MS and the antifungal activates were then performed. Results: We found that △BcSpd1 enhanced the ginseng defense response when applied to healthy ginseng leaves and further changed the metabolism of flavonoids. Compared with untreated plants, the application of △BcSpd1 on ginseng leaves significantly increased the accumulation of p-coumaric acid and myricetin, which could inhibit the fungal growth. Conclusion: B. cinerea △BcSpd1 could effectively induce the medicinal plant defense and is referred to as the biological control agent in ginseng disease management.

키워드

과제정보

This work was partly supported by the National Natural Science Foundation of China (No. 32171801 to S.L.), Jilin Province Science and Technology Development Project of China (No. 20210202095NC to N.L.), and the Cross-Disciplinary Innovation Founding of Jilin University (No. JLUXKJC2020313 to S.L.).

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