• 한국지능시스템학회:학술대회논문집
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• 한국퍼지및지능시스템학회 2003년도 ISIS 2003
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• pp.28-31
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• 2003

In the present work, a main purpose is to propose a fuzzy integral-based aggregation framework to complementarily combine partial information due to lack of completeness. Based on Choquet integral (CI) viewed as monotone expectation, we take into account complementary, non-interactive, and substitutive aggregations of different sources of defective information. A CI-based system representing upper, conventional, and lower expectations is designed far handling three aggregation attitudes towards uncertain information. In particular, based on Choquet integrals for belief measure, probability measure, and plausibility measure, CI$\_$bi/-, CI$\_$pr/ and CI$\_$pl/-aggregator are constructed, respectively. To illustrate a validity of proposed aggregation framework, multiple matching systems are developed by combining three simple individual template-matching systems and tested under various image variations. Finally, compared to individual matchers as well as other traditional multiple matchers in terms of an accuracy rate, it is shown that a proposed CI-aggregator system, {CI$\_$bl/-aggregator, CI$\_$pl/-aggregator, Cl$\_$pl/-aggregator}, is likely to offer a potential framework for either enhancing completeness or for resolving conflict or for reducing uncertainty of partial information.

• 한국식물생명공학회:학술대회논문집
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• 한국식물생명공학회 2002년도 추계학술대회
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• pp.69-75
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• 2002

Sterols play two major roles in plants: a bulk component in biological membranes and precursors of plant steroid hormones. Physiological effects of plant steroids, brassinosteroids (BRs), include cell elongation, cell division, stress tolerance, and senescence acceleration. Arabidopsis mutants that carry genetic defects in BR biosynthesis or its signaling display characteristic phenotypes, such as short robust inflorescences, dark-green round leaves, and sterility. Currently there are more than 100 dwarf mutants representing 7 genetic loci in Arabidopsis. Mutants of 6 loci, dwf1/dim1/cbb1, cpd/dwf3, dwf4, dwf5, det2/dwf6, dwf7 are rescued by exogenous application of BRs, whereas bri1/dwf2 shares phenotypes with the above 6 loci but are resistant to BRs. These suggest that the 6 loci are defective in BR biosynthesis, and the one locus is in BR signaling. Biochemical analyses, such as intermediate feeding tests, examining the levels of endogenous BR, and molecular cloning of the genes revealed that dwf7, dwf5, and dwf1 are defective in the three consecutive steps of sterol biosynthesis, from episterol to campesterol via 5-dehydroepisterol. Similarly, det2/dwf6, dwf4, and cpd/dwf3 were shown to be blocked in $D^4$ reduction, 22a-hydroxylation, and 23 a-hydroxylation, respectively. A signaling mutant bri1/dwf2 carries mutations in a Leucine-rich repeat receptor kinase. Interestingly, the bri1 mutant was shown to accumulate significant amount of BRs, suggesting that signaling and biosynthesis are dynamically coupled in Arabidopsis. Thus It is likely that transgenic plants over-expressing the rate-limiting step enzyme DWF4 as well as blocking its use by BRI1 could dramatically increase the biosynthetic yield of BRs. When applied industrially, BRs will boost new sector of plant biotechnology because of its potential use as a precursor of human steroid hormones, a novel lead compound for cholesterol-lowering effects, and a various application in plant protection.

• 한국멀티미디어학회논문지
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• 제14권7호
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• pp.895-904
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• 2011

기존 스포트 용접을 수행하는 용접기에서 용접 대상물을 공급할 때 발생하는 흔들림 등으로 대상물이 정확한 위치에 놓이지 않는 경우가 허다하다. 또한 작업자나 관련 장비의 이동 중 여러 가지 상황에 노출된 작업환경 하에서는 너트의 상태를 정확하게 판별할 수 없게 된다. 그러므로 너트에 대한 용접 불량이 발생한 경우, 이를 판단할 수 있는 장치가 없는 상태에서 작업자가 일일이 검사해야 하는 문제가 있기 때문에 영상처리를 통한 다양한 평가분석이 필요하다. 따라서 본 연구논문에서는 기존의 정확성 떨어지는 영상처리 시스템을 개선함으로써 대상물이 바르게 안착되지 않은 상태에서도 정확한 인식과 동시에 분석 시간을 줄일 수 있도록 하는 알고리즘 구현을 목적으로 한다. 이와 같이 너트의 안착여부 상태평가를 위한 영상분석인 만큼, 구현된 알고리즘을 그룹별로 나열하고 제시하였으며 실험을 통해서 그 효용성을 나타내었다. 그 결과, 판정시간에 따른 정상 및 오류 인식률이 실험분류((분류1)/그룹1)에서 실험분류((분류11)/그룹5)까지 40%~94.6%와 60%~5.4%로 나타났으며, 최소, 최대, 평균 판정시간은 1.7초~0.08초, 3.6초~1.2초, 2.5초~0.1초로 나타났다.

• 한국식물생명공학회:학술대회논문집
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• 한국식물생명공학회 2002년도 춘계학술대회
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• pp.69-75
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• 2002

Sterols play two major roles in plants: a bulk component in biological membranes and precursors of plant steroid hormones. Physiological effects of plant steroids, brassinosteroids (BRs), include cell elongation, cell division, stress tolerance, and senescence acceleration. Arabidopsis mutants that carry genetic defects in BR biosynthesis or its signaling display characteristic phenotypes, such as short robust inflorescences, dark-green round leaves, and sterility. Currently there are more than 100 dwarf mutants representing 7 genetic loci in Arabidopsis. Mutants of 6 loci, dwf1/dim1/cbb1, cpd/dwf3, dwf4, dwf5, det2/dwf6, dwf7 are rescued by exogenous application of BRs, whereas bri1/dwf2 shares phenotypes with the above 6 loci but are resistant to BRs. These suggest that the 6 loci are defective in BR biosynthesis, and the one locus is in BR signaling. Biochemical analyses, such as intermediate feeding tests, examining the levels of endogenous BR, and molecular cloning of the genes revealed that dwf7, dwf5, and dwf1 are defective in the three consecutive steps of sterol biosynthesis, from episterol to campesterol via 5-dehydroepisterol. Similarly, det2/dwf6, dwf4, and cpd/dwf3 were Shown to be blocked in $D^4$ reduction, 22a-hydroxylation, and 23 a-hydroxylation, respectively. A signaling mutant bri1/dwf2 carries mutations in a Leucine-rich repeat receptor kinase. Interestingly, the bri1 mutant was shown to accumulate significant amount of BRs, suggesting that signaling and biosynthesis are dynamically coupled in Arabidopsis. Thus it is likely that transgenic plants over-expressing the rate-limiting step enzyme DWF4 as well as blocking its use by BRI1 could dramatically increase the biosynthetic yield of BRs. When applied industrially, BRs will boost new sector of plant biotechnology because of its potential use as a precursor of human steroid hormones, a novel lead compound for cholesterol-lowering effects, and a various application in plant protection.

• Journal of Plant Biotechnology
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• 제29권2호
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• pp.139-144
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• 2002

Sterols play two major roles in plants: a bulk component in biological membranes and precursors of plant steroid hormones. Physiological effects of plant steroids, brassinosteroids (BRs), include cell elongation, cell division, stress tolerance, and senescence acceleration. Arabidopsis mutants that carry genetic defects in BR biosynthesis or its signaling display characteristic phenotypes, such as short robust inflorescences, dark-green round leaves, and sterility. Currently there are more than 100 dwarf mutants representing 7 genetic loci in Arabidopsis. Mutants of 6 loci, dwf1/dim1/cbb1, cpd/dwf3, dwf4, dwf5, det2/dwf6, dwf7 are rescued by exogenous application of BRs, whereas bri1/dwf2 shares phenotypes with the above 6 loci but are resistant to BRs. These suggest that the 6 loci are defective in BR biosynthesis, and the one locus is in BR signaling. Biochemical analyses, such as intermediate feeding tests, examining the levels of endogenous BR, and molecular cloning of the genes revealed that dwf7, dwf5, and dwf1 are defective in the three consecutive steps of sterol biosynthesis, from episterol to campesterol via 5-dehydroepisterol. Similarly, det2/dwf6, dwf4, and cpd /dwf3 were shown to be blocked in D$^4$reduction, 22a-hydroxylation, and 23 a-hydroxylation, respectively. A signaling mutant bril/dwf2 carries mutations in a Leucine-rich repeat receptor kinase. Interestingly, the bri1 mutant was shown to accumulate significant amount of BRs, suggesting that signaling and biosynthesis are dynamically coupled in Arabidopsis. Thus it is likely that transgenic plants over-expressing the rate-limiting step enzyme DWF4 as well as blocking its use by BRIl could dramatically increase the biosynthetic yield of BRs. When applied industrially, BRs will boost new sector of plant biotechnology because of its potential use as a precursor of human steroid hormones, a novel lead compound for cholesterol-lowering effects, and a various application in plant protection.

• 한국멀티미디어학회논문지
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• 제14권1호
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• pp.76-84
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• 2011

임베디드 시스템은 적용되는 분야에 따라 태스크의 완료에 대한 실시간성이 보장되어야 하는 경우가 있다. 또한 실시간성을 제공하는 시스템에서는 다양한 이유로 인해 태스크의 결함이 발생할 수 있다. 그러므로 본 논문에서는 단일 프로세서를 가지는 임베디드 시스템에서 주기적 태스크 집합의 마감시간을 보장하면서 결함이 발생한 태스크의 결함을 허용하는 태스크 스케줄러를 설계한다. 임베디드 시스템에서 실시간성을 제공하기 위해서 태스크를 주기적 및 비주기적 특성으로 분류한 후 주기적 태스크는 고정 우선순위 실시간 태스크 스케줄링 기법에서 최적의 알고리즘으로 알려진 RMS(Rate Monotonic Scheduling) 기법을 적용하여 실행한다. 주기적 태스크들의 실행 시간을 분석한 후, 결정되는 잉여 시간을 관리함으로써 비주기적 태스크들의 실행을 보장한다. 또한 일시적인 태스크의 단일 결함을 허용하기 위한 결함 허용 기법을 제공한다. 이를 위해 백업 시간을 관리하고 태스크에서 결함이 발생할 경우, 결함이 발생한 태스크를 재실행함으로써 복구 작업을 수행한다.

• 한국동물번식학회:학술대회논문집
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• 한국동물번식학회 2001년도 춘계학술발표대회
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• pp.62-62
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• 2001

Recently, production of transgenic animal by nuclear transfer has been known as a useful method. The production of cloned offspring derived from nuclear transfer depends upon a variety of factors such as species, donor cells type and cell cycle, and source of recipient ova. Therefore, we attempted a different transgenic methods using follicular granulosa cells (GCs). In general, ovulated GCs undergoes lutenization and transformation in vitro which might defective effects on developmental potential. In order to avoid the GCs transformation in vitro culture system, we introduced a direct injection of retrovirus into the follicles and then collected them mechanically from ovaries of 6-8 week-old ICR mice. Retrovirus vector constructed with pLN $\beta$ EGFP was injected into the follicles. The follicles are cultured in $\alpha$ -MEM supplemented with human FSH, LH and ITS in Costar Transwell dish for 4 days. Survival rate of virus injected follicles was 52.1% (12/23) and expression rate of EGPP gene was 33.3% (4/12). In this study, we found GCs performed transgenesis in our culture system. In addition, the GCs in follicle may be developed in vivo like environment rather than in vitro environment. Thus, the use of GCs as donor cells may be useful in the nuclear transfer for cloning of genetic modification. Therefore, these results suggest that follicular GCs can be transfected by viral vector during folliculogenesis in vitro.

• 한국융합학회논문지
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• 제9권10호
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• pp.257-263
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• 2018

본 연구는 트랜스미션에 들어가는 부품인 차동기어에 대한 자동 검사 장비를 개발하고자 하는 것이다. 본 기술개발은 Micro Vision 자동 검사 장비와 마이크로레이저 자동 검사 장비를 사용하여 만들 것이다. 이는 작업자가 부주의하게 가공한 제품을 전수검사 단계에서 불량률 0를 만들고자 한다. 본 연구를 통해 개발된 장비를 여러 분야 사업에 적용할 것이다. 포장회사, 너트 볼트 가공업체, 정밀 반도체 상단에 인쇄를 위한 업체, SMT 업체 등 다양한 분야에 비전검사 장비를 판매하고자 한다. 본 기술개발을 통해 불량률 0를 실현하면 모기업으로부터 안정적 물량 확보, 나아가 제품 신뢰도를 바탕으로 수출을 할 수 있는 기반마련의 기회도 가능하다. 자동검사시스템을 국내 자동차 부품 가공업체에 적용할 경우 우리나라 자동차의 신뢰도 또한 크게 상승 할 것으로 생각된다.

• BMB Reports
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• 제29권4호
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• pp.286-293
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• 1996

Association of antigenic peptide with class I MHC is believed to be crucial for maintaining stable conformation of class I molecules. T2 cells that are defective in TAP gene function mainly express class I molecules with an unstable conformation due to little or no association with antigenic peptides, whereas T1 cells that are normal in TAP gene function mainly express the stable form of class I molecules. In this work, attempts were made to determine the molecular stability of stable and unstable class I molecules. Dissociation of HLA-A2 molecules on T1 and T2 cells was monitored by flow cytometry using anti-HLA-A2 antibody after the cells were treated with brefeldin A to shut down the transport of newly-assembled HLA-A2. Estimated dissociation rate constants for the stable and unstable forms of HLA-A2 were 0.076 $h^{-1}$ and 0.66 $h^{-1}$, respectively. It appeared that both T1 and T2 cells express stable and unstable class I complex, but with different ratios of the two forms. Furthermore, $interferon-{\gamma}$ treatment of T1 cells appeared to induce the expression of both the stable and unstable class I molecules. These results demonstrate that class I MHC molecules can be divided into two groups in terms of structural stability and that they exist on the cell surface in both forms in a certain ratio.

• 한국동물위생학회지
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• 제30권3호
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• pp.291-304
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• 2007

Mutants of an obligate aerobic bacterium, Vitreoscilla, that have deficiency in heat-labile catalase-peroxidase hydroperoxidase I (HPI) were created by EMS treatment. The catalase-peroxidase HPI-deficient mutant showed substantially lower peroxidase activity in exponential and mid-stationary phase compared with the wild type strain. In late stationary phase, the mutant exhibited no peroxidase activity. Peroxidase deficiency in the mutant was revealed by polyacrylamide gels stained for peroxidase activity. Characteristically, catalase levels in the mutant increased about 14- and 8-fold during growth in the exponential and stationary phases, respectively, compared to those in the wild type, suggesting a compensatory effect for protection from $H_2O_2$ toxicity. The mutant showed differences in physiology from the wild type: retardation in growth rate and decrease in oxygen consumption. Both the wild type and the catalase-peroxidase HPI-deficient mutant of Vitreoscilla had lower growth rates in media containing increasing $H_2O_2$ concentrations. However, the mutant exhibited an additionally decreased growth rate after 6 to 8 h of growth compared to the wild type. The wild type was resistent up to 20 mM $H_2O_2$, whereas the mutant was very sensitive to high concentrations of exogenous $H_2O_2$. Although elevated catalase levels would provide protection of the bacteria from the deleterious effect of $H_2O_2$, it did not appear to be complete. Cell-free extracts of the mutant showed decreased NADH oxidation rates and higher accumulation of $H_2O_2$ during this oxidation. These results may account for the impaired growth and earlier onset of death phase by the catalase-peroxidase HPI-deficient mutant of Vitreoscilla.