The longitudinal changes in transitional milk intakes and body weight of infants(18 boys, 10 girls) have been studied in the part of Kangwon Province from 7 days to 15 days postpartum. Milk intakes at 7, 10, 15 days postpartum were 474$\pm$155, 524$\pm$154 and 547$\pm$130g, respectively. The mean milk intakes were 515g from 7 to 15 days postpartum. Milk intakes of boys were significantly higher than those of girls. The transitional milk intakes per weight of infants appeared 147$\pm$41g/kg. The number of feed per day of boys were significantly higher than those of girls. Milk intakes per fed were 56$\pm$20g. The transitional milk intakes were affected by gestational period(p<0.05), maternal height(p<0.05) and mother's transitional milk yields (p<0.001). Infants transitional milk intakes were 82.6$\pm$17.0% of mothers transitional milk yields. Infants gained its weight 24.6, 40.1 and 39.0g/day at, 7, 10, 15 days postpartum, respectively.
This experiment was carried out to study effects fo dosage and insemination interval on fertility and hatchability of whole semen and diluted semen with yolk skim milk and skim milk dilutors. The results obtained are as follows: 1. Whole semen showed higher fertility than diluted semen with yolk skim milk and skim milk dilutors. In case of diluted semen, the fertility was higher in 0.04$m\ell$ dosage than 0.02$m\ell$ and in skim milk than yolk skim milk dilutor. 2. The average fertility in inseminational intervals of 6, 5, 4 and 3 days was 52.4, 35.5, 48.7 and 44.2% in whole semen and 40.6, 17.2, 13.9 and 20.5% in 0.04$m\ell$ diluted semen with yolk skim milk dilutor. The fertility was not improved by shortening of insemination interval. 3. There was no considerable difference in hatchability of fertilized egg among the dosage of 0.02$m\ell$ of whole semen, 0.02$m\ell$ and 0.04$m\ell$ of diluted semen with yolk skim milk, and among the insemination intervals of 6, 5, 4 and 3 days. 4. Some differences in fertility among the passed days after insemination were decreased in the whole semen by shortening insemination interval from 6 and 5 days to 4 and 3days and also decreased in the diluted semen by shortening it to 3 days and by increasing dosage from 0.02$m\ell$ to 0.04$m\ell$. 5. Hatchability of fertilized egg showed no difference among the passed days during 6 days insemination interval both in the whole semen and the diluted semen. 6. The whole semen and the diluted semen with skim milk had not considerable difference in fertility among the passed days during 3 and 4 days insemination intervals, but the diluted semen with yolk skim milk had. 7. Hatchability of fertilized egg from the whole semen diluted semen with yolk skim milk and skim milk dilutors showed no difference among the passed days during 3 and 4 days insemination intervals.
The longitudinal changes in the transitional milk yields of Korean lactating women(14 primiparae, 11 multiparae) have been studied by test-weighing method in the part of Kangwon Province form 7 day to 15 days postpartum. The transitional milk yields at 7, 10, 15 days postpartum were 531$\pm$148,598$\pm$156 and 639$\pm$169g, respectively. The mean milk yield was 589$\pm$162g from 7 to 15 days postpartum. The transitional milk yields between primiparae and multiparae appeared not significantly different but significantly different between mothers of boys and girl(p<0.05). The distribution of individual transitional milk yields were found 550-649g(28.0%), 450-549g(24.0%), 650-749g(13.3%) and 750-849g(13.3%). The transitional milk yields were not affected by mother's age, weight gain during pregnancy, gestational period and infant's weight at birth but affected by maternal height(p<0.05). Maternal weight reduction during the lactation had no correlation with the transitional milk yeilds.
This study was conducted to investigate the quality changes of the UHT(ultra-high temperature), LTLT(law temperature long time) and HTST(high temperature short time) treated milk samples by storage conditions for 6 months from August 2000 to February 2001. The UHT treated milk samples collected from 3 plants(A, B and C) were stored at l0$^{\circ}$C and room temperature(dark and light exposure) for 6 months, and the LTLT and HTST treated milk samples(D and E) were also stored for 30 days. The UHT pasteurized milk of A, B and C plant was treated at 130$^{\circ}$C for 2-3s, 133$^{\circ}$C for 2-3s and 135$^{\circ}$C for 4s, respectively. The UHT sterilized milk of A and B plant was treated at 140$^{\circ}$C for 2-3s and 145$^{\circ}$C for 3-4s, respectively. The LTLT milk of D plant was treated at 63$^{\circ}$C for 30 mins, and the HTST milk of E plant was treated at 72$^{\circ}$C for 15s. All of the raw milk samples collected from storage tank in 5 milk plants were showed less than 4.0 X 10$^5$cfu/ml in standard plate count, and normal level in acidity, specific gravity, and component of milk. Preservatives, antibiotics, sulfonamides and available chloride were not detected in both raw and heat treated milk samples obtained from 5 plants. One(10%) of 10 UHT pasteurized milk samples obtained from B plant and 2 (20%) of 10 from C were not detected in bacterial count after storage at 37$^{\circ}$C for 14 days, but all of the 10 milk samples from A were detected. No coliforms were detected in all samples tested. No bacteria were also detected in carton, polyethylene and tetra packs collected from the milk plants. A total of 300 UHT pasteurized milk samples collected from 3 plants were stored at room(3$^{\circ}$C ${\sim}$ 30$^{\circ}$C) for 3 and 6 months, 11.3%(34/300) were kept normal in sensory test, and 10.7%(32/300)were negative in bacterial count. The UHT pasteurized milk from A deteriorated faster than the UHT pasteurized milk from B and C. The bacterial counts in the UHT pasteurized milk samples stored at 10$^{\circ}$C were kept less than standard limit(2 ${\times}$ 10$^4$ cfu/ml) of bacteria for 5 days, and bacterial counts in some milk samples were a slightly increased more than the standard limit as time elapsed for 6 months. When the milk samples were stored at room(3$^{\circ}$C ${\sim}$ 30$^{\circ}$C), the bacterial counts in most of the milk samples from A plant were more than the standard limit after 3 days of storage, but in the 20%${\sim}$30%(4${\sim}$6/20) of the milk samples from B and C were less than the standard limit after 6 months of storage. The bacterial counts in the LTLT and HTST pasteurized milk samples were about 4.0 ${\times}$ 10$^3$ and 1.5 ${\times}$ 101CFU/ml at the production day, respectively. The bacterial counts in the samples were rapidly increased to more than 10$^7$ CFU/ml at room temperature(12$^{\circ}$C ${\sim}$ 30$^{\circ}$C) for 3 days, but were kept less than 2 ${\times}$ 10$^3$ CFU/ml at refrigerator(l0$^{\circ}$C) for 7 days of storage. The sensory quality and acidity of pasteurized milk were gradually changed in proportion to bacterial counts during storage at room temperature and 10$^{\circ}$C for 30 days or 6 months. The standard limit of bacteria in whole market milk was more sensitive than those of sensory and chemical test as standards to determine the unaccepted milk. No significant correlation was found in keeping quality of the milk samples between dark and light exposure at room for 30 days or 6 months. The compositions of fat, solids not fat, protein and lactose in milk samples were not significantly changed according to the storage conditions and time for 30 days or 6 months. The UHT sterilized milk samples(A plant ; 20 samples, B plant ; 110 samples) collected from 2 plants were not changed sensory, chemical and microbiological quality by storage conditions for 6 months, but only one sample from B was detected the bacteria after 60 days of storage. The shelflife of UHT pasteurized milk in this study was a little longer than that reported by previous surveys. Although the shelflife of UHT pasteurized milk made a significant difference among three milk plants, the results indicated that some UHT pasteurized milk in polyethylene coated carton pack could be stored at room temperature for 6 months. The LTLT and HTST pasteurized milk should be sanitarily handled, kept and transported under refrigerated condition(below 7$^{\circ}$C) in order to supply wholesome milk to consumers.
Milk components analysis was carried out milk yield(MY), milk fat(MF), milk protein(MP), milk urea nitrogen(MUN), milk solid(MS), day of non-pregnant condition(DNPC), and days of primipara(DPRI) involved. Dairy farms were divided high, middle and low groups according to the standard records for milk components. Examination records were divided by farm, parity, year, season and month, the number of samples were 28,957. MUN concentration was below 12 mg and when the MPP was below 3.0%, the days of non-pregnant condition were $94{\pm}10.77$ days but concentration of MUN was under 12 mg and when MPP was above 3.2%, longer period of non-pregnant condtion of $181.3{\pm}9.25$ was noted. The days of gestation of the first calving cow was $495.9{\pm}9.04$ days when the concentration of MUN was below 12mg/dl and MPP was under 3.0%. If the concentration of MUN was 12 mg/dl and when the MPP was over 3.2%, the days of gestation were $511.0{\pm}8.36$ days. It was believed that the concentrations of MPP and MUN have significant effects on the days of non-pregnant condition and the days of gestation. Determination of MY, MF, MS, and MUN was Milkoscan $4,000{\sim}5,000$ Serier(FOSS Electric Co., Copenhagen, Denmark). MUN standard concentration was 12-18(mg/dl) similar to blood urea nitrogen(BUN). Mutual relationship of milk components(MF, SCC, MY, DNPC, MS) and MUN concentration was low in regression analysis.
Skim milk progesterone($P_4$) profiles in 74 dairy cows were determind to monitor postpartum ovarian activity by radioimmunoassay. Milk samples were collected from each cow every 5 days from 10 to 90 days postpartum. Signs of estrus were observed twice daily, and status of the ovaries and uterus were examined every 10 days by rectal palpation. Results are summarized as follows: 1. Cows were categorized into five types by the change of skim milk $P_4$ profiles; Type I(normal) : Cyclic changes of skim milk $P_4$ profiles appeared within 20 days postpartum(12 cows, 16.2%), Type II(cycle delayed) : Cyclic changes of skim milk $P_4$ profiles appeared from 21 to 60 days postpartum(39 cows, 52.7%), Type III(cycle ceased with low $P_4$) : Onset of the estrous cycle within 20 days postpartum but ceased later with low levels of $P_4$ (7cows, 9.5%), Type IV(cycle ceased with high $P_4$) : Onset of the estrous cycle within 20 days postpartum but ceased later with high levels(>3.0 ng/ml) of skim milk $P_4$ (4 cows, 5.4%), Type V(acyclicity) : Skim milk $P_4$ concentration remained low(<1.0 ng/ml) until 80 days postpartum(12 cows, 16.2%). 2. Out of the 17 cows classified as the Type III and Type V by skim milk $P_4$ profiles, 13 cows had inactive ovaries and remaining 6 cows had single or multiple follicular cysts in their ovaries by rectal palpation. All 4 cows of Type IV had a persistent corpus luteum in their ovaries. 3. Approximately eighty percent of the cows had begun ovarian activity by 60 days postpartum and 90.6% by 90 days by skim milk $P_4$ profiles, but only 39.2% by 60 days and 71.7% by 90 days had shown visible estrus signs. The mean days from parturition to the first, second and third ovulations determined by skim milk $P_4$ profiles was $28.0{\pm}11.0$, $46.4{\pm}13.3$ and $66.4{\pm}11.5$ days and the visible estrus signs were 9.3%, 38.1%, and 48.6%, respectively. The mean days from parturition to the first visible estrus was $57.2{\pm}15.9$ days. These results indicated that milk $P_4$ profile of each Types by radioimmunoassay can be utilized for monitoring postpartum ovarian and would be useful for the early detection of ovarian dysfunction in dairy cow.
A random regression model was applied for the first time for the analysis of test day records and to study the genetic persistency of first lactation milk yield of Indian Murrah buffaloes. Wilmink's Function was chosen to describe the shape of lactation curves. Heritabilities of test day milk yield varied from 0.33 to 0.58 in different test days. The highest heritability was found in the initial test day ($5^{th}$ day) milk yield. Genetic correlations among test day milk yields were higher in the initial test day milk yield and decreased when the test day interval was increased. The magnitude of genetic correlations between test day and 305 day milk yield varied from 0.25 to 0.99. The genetic persistencies of first lactation milk yield were estimated based on daily breeding values using two methods. $P_1$ is the genetic persistency estimated as a summation of the deviation of estimated daily breeding value on days to attain peak yield from each day after days to attain peak yield to different lactation days. $P_2$ is the genetic persistency estimated as the additional genetic yield (gained or lost) from days to attain peak yield to estimated breeding value on different lactation days relative to an average buffalo having the same yield on days to attain peak yield. The mean genetic persistency on 90, 120, 180, 240, 278 and 305 days in milk was estimated as -4.23, -21.67, -101.67, -229.57, -330.06 and -388.64, respectively by $P_1$, whereas by $P_2$ on same days in milk were estimated as -3.96 (-0.32 kg), -23.94 (-0.87 kg), -112.81 (-1.96 kg), -245.83 (-2.81 kg), -350.04 (-3.28 kg) and -407.58 (-3.40 kg) respectively. Higher magnitude of rank correlations indicated that the ranking of buffaloes based on their genetic persistency in both methods were similar for evaluation of genetic persistency of buffaloes. Based on the estimated range of genetic persistency three types of genetic persistency were identified. Genetic correlations among genetic persistency in different days in milk and between genetic persistencies on the same day in milk were very high. The genetic correlations between genetic persistency for different days in milk and estimated breeding value for 305 DIM was increased from 90 DIM to 180 DIM, and highest around 240 DIM which indicates a minimum of 240 days as an optimum first lactation length might be required for genetic evaluation of Indian Murrah buffaloes.
The changes of selenium and zine contents of transitional milk and mature milk at 7 and 60 days postpartum were comparatively studied on 20 Korean lactating women. the selenium and zinc contents of transitional milk and mature milk were analyzed by atomic absorption spectrophotometer after wet-digestion. The mean selenium contents wer 12.2$\mu\textrm{g}$ /ι and 11.5$\mu\textrm{g}$/ι at 7 and 60 days postpartum, respectively. The mean zinc contents decreased significantly from 4.23mg/ι at 7 days to 1.62mg/ι at 60 days postpartum (p<0.05).
Data containing 14,188 lactation and reproductive records of Korean Holstein cows at first parity distributed across 3,734 herd-year-season groups were analyzed to get genetic (co)variance estimates for milk yield, fat yield, calving ease, and days open. Milk and Fat yields were adjusted to 305 d. Heritabilities and genetic correlations were estimated in two different animal models on which were included direct genetic effects (Model 1) and direct+maternal genetic effects (Model 2) using REML algorithms. Milk and fat yields were affected by age at first calving as linear and quadratic. Heritability estimates of direct effects were 0.25 for milk yield, 0.17 for fat yield, 0.03 for calving ease and 0.03 for days open in Model 2. These estimates for maternal effects were 0.05, 0.08, 0.04 and less than 0.01 for each corresponding trait. Milk productions at first lactation were to show genetically favorable correlation with calving ease and days open for direct genetic effects (-0.24 - -0.11). Moreover, calving ease was correlated with days open of 0.30 for direct genetic effects. Correlations between direct and maternal effects for each trait were negatively correlated (-0.63 - -0.32). This study suggested that maternal additive genetic variance would be not ignorable for genetic evaluation of milk production as well as reproductive traits such as calving ease and days open at first parity. Furthermore, difficult calving would genetically influence the next conception.
The study was conducted on six multiparous Murrah buffaloes which were earlier artificially induced into lactation. During the experimental period of 15 days, buffaloes were managed in a loose housing system. All the buffaloes were administered a single injection of bromocryptine (@ $100{\mu}g/kg$ body weight) subcutaneously in the neck region at 08:30 A.M., 50 days postpartum (early lactation). Blood samples were collected from four buffaloes for a period of 5 days before the administration of bromocryptine i.e. on days -5, -4, -3, -2, -1, on day of treatment (day 0) and thereafter daily for a period of 9 days i.e 1, 2, 3, 4, 5, 6, 7, 8 and 9 to determine the hormones and blood metabolites. Homogeneous milk samples from all the buffaloes were collected at morning and evening milkings on days coinciding with the days of blood sampling for analysis of milk constituents. Administration of bromocryptine resulted in a significant inhibition of plasma prolactin within 24 hrs of treatment, but the response in all the buffaloes was not uniform. The effect of bromocryptine on plasma prolactin hormone lasted for 1-4 days but Cortisol concentration were not altered. Administration of bromocryptine neither affected blood glucose nor plasma non-esterified fatty acids concentration. Irrespective of level of milk production from different buffaloes, there was no effect of bromocryptine on milk yield which indicated that prolactin is not required for milk secretion during early lactation in buffaloes. Milk constituents like fat, protein and lactose were not affected by bromocryptine may be due to no effect of bromocryptine of milk yield.
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