• Toxicological Research
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• 제31권1호
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• pp.77-88
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• 2015

Lithospermum erythrorhizon has long been used in traditional Asian medicine for the treatment of diseases, including skin cancer. The oral toxicity of a hexane extract of Lithospermum erythrorhizon root (LEH) was investigated in Beagle dogs by using single escalating doses, two-week dose range-finding, and 4-week oral repeat dosing. In the single dose-escalating oral toxicity study, no animal died, showed adverse clinical signs, or changes in body weight gain at LEH doses of up to 2,000 mg/kg. In a 2 week dose range-finding study, no treatment-related adverse effects were detected by urinalysis, hematology, blood biochemistry, organ weights, or gross and histopathological examinations at doses of up to 500 mg LEH/kg/day. In the 4 week repeat-dose toxicity study, a weight loss or decreased weight gain was observed at 300 mg/kg/day. Although levels of serum triglyceride and total bilirubin were increased in a dose dependent manner, there were no related morphological changes. Based on these findings, the sub-acute no observable adverse effect level for 4-week oral administration of LEH in Beagles was 100 mg/kg/day.

• 대한의생명과학회지
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• 제7권3호
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• pp.111-116
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• 2001

To investigate an effect of aging on the xylene metabolism in liver damaged animals, a study was conducted. 50% carbon tetrachloride ($CCl_4$) in olive oil (0.1 ml/100 g body weight) was intraperitoneally given to 5-week and 12-week rats 12 times every other day and then one dose of 50% xylene in olive oil (0.25 ml/100 g body weight) was intraperitoneally given to the rats, and after 24 hr, the animals were sacrificed. On the basis of the functional findings in rat liver, ie, serum levels of alanine aminotransferase activity, liver protein and malonedialdehyde contents, 5-week rats showed less liver damage than 12-week rats. The increasing rate of urinary methylhippuric acid concentration to the control was significantly higher in 5-week rats than 12-week rats in case of xylene treatment after induction of liver damage. On the other hand, liver damaged 5-week rats showed significant rise of hepatic cytochrome P45O content compared with the liver damaged 12-week rats by the xylene treatment. And increasing rate of hepatic alcohol or aldehyde dehydrogenase activities to each liver damaged animals was higher tendency in 5-week rats than 12-week rats by the xylene treatment. In conclusion, 5-week rat showed greater metabolic rate of xylene than 10-week rats in case of liver injury because 5-week rats led to a slight liver damaged compared with 12-week rats.

• Nutrition Research and Practice
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• 제11권1호
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• pp.33-42
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• 2017

BACKGROUND/OBJECTIVE: This study aims to measure the in vitro antioxidant capacity of Korean diet (KD) with American diet (AD) as a control group and to examine the ex vivo DNA damage reduction effect on human lymphocytes. MATERIALS/METHODS: The KD applied in this study is the standard one-week meals for Koreans (2,000 kcal/day) suggested by 2010 Dietary Reference Intakes for Koreans. The AD, which is the control group, is a one-week menu (2,000 kcal/day) that consists of foods that Americans would commonly take in according to the National Health and Nutrition Examination Survey. The antioxidant capacity of each menu was measured by means of the total phenolic assay and 3 in vitro antioxidant activity assays (2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, trolox equivalent antioxidant capacity (TEAC), Oxygen radical absorbance capacity ($ORAC_{ROO{\cdot}}$)), while the extent of ex vivo lymphocyte DNA damage was measured by means of the comet assay. RESULTS: When measured by means of TEAC assay, the in vitro antioxidant capacity of the KD of the day was higher than that of the AD (P < 0.05) while there was no significant difference in total phenolic contents and DPPH and ORAC assays. The ex vivo lymphocyte DNA damage protective effect of the KD was significantly higher than that of the AD (P < 0.01). As for the one-week menu combining the menus for 7 days, the total phenolic assay (P < 0.05) and in vitro antioxidant capacity (P < 0.001, DPPH; P < 0.01, TEAC) of the KD menu were significantly higher than those of the AD menu. Likewise, the ex vivo DNA damage reduction rate of the Korean seven-day menu was significantly higher than that of the American menu (P < 0.01). CONCLUSION: This study demonstrates that the high antioxidant capacity and DNA damage protective effect of KD, which consists generally of various plant foods, are higher than those of typical AD.

• 재무관리연구
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• 제20권2호
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• pp.201-234
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• 2003

본고에서는 세계시장 중에서 주요 7 국가의 시장을 대상으로 시장의 이례현상의 하나인 주말 효과와 주중 일별수익률에 대한 연구를 결제청산과정으로 접근 분석하였다. 결제청산과정은 특정한 날의 일별수익률에 영향을 미칠 수 있고 또한 시장마다 서로 다른 결제청산체계는 세계 각 시장에서 특정한 날의 일별수익률에 서로 다르게 영향을 미칠 수 있다. 최근 이러한 결산청산체계 자체가 미국, 영국 프랑스 등 여러 주요시장에서 변화가 일어났다. 그리하여 본고에서는 세계 각 국가시장에서 주식의 결제청산과정체계의 변화 및 전환에 따라 주말효과 및 주중 일별수익률의 변화에 미치는 요인을 분석하였다. 그리고 또한 주말효과와 같은 시장효율성의 이례 현상에 대한 최근 연구에서 그전과 다른 기류에 대한 연구결과가 보고되기 시작하였다. 그리하여 그전의 자료와 최근의 10여 년간의 자료를 나누어 세계 각 주요시장의 주말효과에 대한 연구를 재검토 할 필요가 있었다. 본고에서 분석한 결과 1990년대 이전에는 각 시장마다 주말효과가 강하지만 최근 10여 년 동안의 자료를 분석한 결과 주말효과가 나타난 국가의 시장은 거의 없었다. 그리고 본고에서 결제청산과정체계의 변화 및 전환이 주말효과 및 주중 일별수익률의 미치는 영향을 분석한 결과 결제청산과정의 종류나 또는 각 시장 상황에 따라 정도의 차이는 있지만 대체적으로 결제청산과정의 변화가 특정한 날의 일별 수익률 패턴에 영향을 미치고 또한 주말효과의 수준의 변화의 일부분은 이러한 결제청산과정의 영향을 받았다고 볼 수 있었다.

• 복식
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• 제64권2호
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• pp.35-49
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• 2014

In cases of fashion businesses operating by consignment, base estimate on quantity of sales is the most essential part of merchandising. This study classified factors influential to sales into factors with systematic influence and factors with unsystematic influence. In order to find out influence of each factor on sales, non-linear regression was used with SPSS package on the basis of actual data on sales for 5 years for sport shoes brand. Major findings of this study are as follows. First, price level had significant negative(-) influence on sales. Second, price expectation effects had significant negative(-) influence on sales. Third, competitor's price effect showed significant negative(-) value. Fourth, day-of-the-week effect showed significant positive(+) effect. The theoretical marketing implications of this study are as follows. First, study on price leads to expansion of the researches from apparels to sport shoes. Field of study on price was enlarged through expansion of variable of study from price level and price expectation effect to promotion, day-of-the-week effect and rainfall effect. Second, quantitative scale of day-of-the-week effect was found and it could be confirmed that there was seasonal differences with day-of-the-week effect. Implications of above findings on marketing managers are as follows. First, it was found that an increase in competitiveness of brand power and a decline in absolute value of competitor's price effect can be realized when new product groups are developed to meet the unsatisfied needs in the market. Second, it was possible to find out the parameters scales of the price response function, making it possible to estimate sales for the next season, and in turn realize increase in rate of sales and profit rate. This research is based on the dynamic price response function, which is rare to find in the apparel business and it academic significance due to its expanding response model which was focused on price in conventional researches to non-systematic variables.

• Biomolecules & Therapeutics
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• 제2권2호
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• pp.190-205
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• 1994

This study was conducted to investigate the repeated dose toxicity of DA-125, a new anthracycline antitumor antibiotic, in rats. Before the 13-week main study, a 4-week dose-range finding (DRF) study was carried out. The administration of DA-125 intravenously at dosage levels of 0, 0.125, 0.5, 2.0, and 8.0 mg/kg/day to rats for 4 weeks resulted in premature deaths of all animals in the 8.0 mg/kg/day group and in the deaths of 4 males and 4 females at 2.0 mg/kg/day. Body weights were markedly reduced in the 8.0 mg/kg/day group and showed dose-related decreases in all treatment groups when compared with the control group. Reductions in weight gain were slight and not significantly different at 0.125 mg/kg/day but animals receiving 0.5 mg/kg/day showed more marked decreases in gain in a clear dose-related manner Based On the results of the above DRF study, a 13-week repeated dose intravenous toxicity study in rats with DA-125 was performed at a dose level of 0, 0.012, 0.08 and 0.3 mg/kg/day. No treatment related effects were noted in behavior or body weight in all treatment groups. One male at the highest dose level died on study day 26, but the death could not be related to test article toxicity. Swelling and scabbing of the ears was present in all of the groups, including the control group. There were no treatment related changes in the hematological, biochemical or urinalysis values in all treatment groups. Thymus weights were significantly reduced ill males receiving 0.3 mg/kg/day and they were sligltly, and not significantly, reduced in females of the same group. While there were no associated histological changes. Treatment related necrosis was found in the tail vein (injection site) at 0.08 and 0.3 mg/kg/day. On the basis of these results, the no observed effect level (NOEL) was 0.012 mg/kg/day and the maximum tolerated dose (MTD) was estimated to be more than 0.3 mg/kg/day under the conditions tested.

• 생물환경조절학회지
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• 제12권1호
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• pp.1-6
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• 2003

본 실험은 정식 후 단일처리전 장일처리 기간에 따른 온실재배 스프레이 절화국 Reagan Improved품종의 건물생산과 절화품질에 미치는 영향을 구명하기 위하여 네덜란드 Wageningen 대학의 유리온실 실험포에서 수행하였다. 정식 13-14일전에 피트블록에 삽목한 묘를 단위면적당 64줄기로 2000년 9월 6, 13, 20일에 각각 정식하였고, 2000년 9월 27일 모든 처리구에 단일처리를 실시하였다. 정식에서 최종조사일까지 장일처리 기간 3주 처리구에서는 84일이었고, 2주 처리구에서는 77일, 1주 처리구에서는 70일, 단일처리 후에 최종수확일까지는 63일이 소요되었다. 개화시기는 모든 처리구에서 동일하였다. 3주 장일처리구에서의 주당 꽃송이수는 1주 장일처리구에 비해 2개 더 많았고, 꽃의 생체중 및 건물중은 각각 식물 개체당 4g과 0.4g더 무거웠지만, 각 처리구별 꽃의 품질에는 큰 차이가 없었다. 최종건물생산량 ($g{\cdot}m^{-2}$ )은 3주 장일처리구에서 높고, 1주 장일처리구에서 가장 낮게 나타났다. 본 실험에서 단일처리 전 장일기간은 단일처리 후 개화시기에 영향을 주지 않았으며 꽃의 품질면에서도 큰 차이가 없었다. 반면 초장과 생체중은 장일 조건이 길수록 높았게 나타났으며, 누적 건물중도 3주 장일처리구에서 가장 높은 결과를 나타냈다. 장일처리 기간은 꽃 자체의 품질에 영향은 적었지만, 개체 생체중 및 초장에 매우 큰 영향을 미쳤으므로 단일처리전 장일처리 기간은 개체품질 및 연중재배 생산 시스템에서 균일한 품질을 생산하기 위해 장일처리 기간의 조절은 고품질의 국화생산에 중요한 요인으로 생각되었다..

• Journal of Animal Science and Technology
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• 제65권2호
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• pp.351-364
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• 2023

The experiment was carried out to study the effect of Korean wild ginseng adventitious root supplementation on the laying performance, egg quality, cytokine expression, ginsenoside concentration, and microflora quantity of Institut de selection Animale (ISA) brown laying hens at 24 weeks old. A total of 90 laying hens were subjected to a completely randomized design at three treatments, five repetitions and six laying hens per replicate. The experiments were divided by diets into the basic feed (CON), basic feed + 0.1% wild ginseng (WG1), and basic feed + 0.5% wild ginseng (WG2). The feeding trial was carried out over a duration of 12 weeks after an initial acclimation period of 2 weeks. Feeds and water were administered ad libitum in mash form, and light was available for 16 hours per day. At the end of study, henday egg production (HDEP), average egg weight (AEW), and egg mass (EM) were increased (p <0.05) in WG2 at week 12. Feed conversion ratio (FCR) was decreased (p < 0.05) in WG2 at week 12. The ginsenoside content in egg yolk was increased (p <0.05) in laying hens in the WG2 treatment at week 12. Relative expression of tumor necrosis factor alpha (TNF-α) was reduced (p < 0.05) in the WG supplemented diets at week 12. The fecal microflora quantity of Lactobacillus was increased (p < 0.05) in WG2 at week 8 to week 12, and Escherichia coli (E. coli) was significantly decreased (p < 0.05) in the WG2 at week 12. We concluded that the result observed in the HDEP, AEW, EM and FCR was due to an increase in ginsenoside content, leading to an improvement in the TNF-α, and fecal microflora quantity such as Lactobacillus and E. coli in the WG2 supplemented diets. We therefore recommend the use of WG at application level 0.5% per basal diet for optimum laying performance in layer hens.

• The Journal of Korean Physical Therapy
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• 제12권1호
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• pp.9-13
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• 2000

The purpose of this study was to determine the overflow effect and cross training effect of isometric quadriceps training that performed in specific angle of unilateral let. Ten healthy students with an average age of 24 years$(24.1\pm1.3)$, were participated in this study. Then 5 subjects in each group were chosen at random to train using only right quadriceps muscle two time per day(group 2), five times a week and the other 5 subjects(group 1) were chosen to train one times per day, five times a week for 2 weeks at only 50 degrees (contract 6 seconds, rest 10 seconds, 3 sets). Before and after the training, isometric quadriceps muscle testing of the both leg was Performed at three different angles, 60, 50 and 40 degrees respectively by BHN-COM (isokinetic dynamometer) in sitting position. The data was analyzed with paired t-test to determine significant difference between before and after training. In this study, we have found that the isometric quadriceps muscle training on specific angle of right side produced overflow effect In healthy subjects. However, increasing the peak torque of specific angle(training angle) of trained limb did not have an effect on increasing the peak torque of contralateral limb. These results demonstrate that the cross training effect did nut found in this study but a alight increase of peak torque of the untrained limb would recognized the possibility of cross training effect.

• Toxicological Research
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• 제14권2호
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• pp.217-226
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• 1998

SDK (skin decontamination kit) is new skin decontaminant which is developed by ADD (Agency for defence development). In this study, four-week toxicity of SDK was investigated using beagle dogs and Sprague-Dawley rats. The beagle dogs and Sprague-Dawley rats were dressed topically seven days per week for 28 days, with dosage of 0, 0.25, 0.8 and 1 g/kg/day. respectively. Animals treated with SDK did not cause any death and show any clinical signs. They did not show any significant changes of body weight, feed uptake and water consumption. They were not significantly different from the control group in urinalysis, ocular examination and histopathological examination. In hematological and serum biochemical assay, there were no-dose-defendent changes. Therefore, SDK was not indicated to have any toxic effect in the beagle dogs and Sprague-Dawley rats when it was dressed topically below the dosage 1 g/kg/day for four weeks.