• Journal of Plant Biology
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• 제20권2호
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• pp.63-69
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• 1977

Experiments were conducted to investigate the effects of several hormones on the growth of suspension culture of carrot (Daucus carota L.) cells, where changes in pH and the amount of $NH_4-Nin$ the medium were observed with regard to growth. A treatment with 2,4-D at $10^{-5}M$ resulted in a highest rate of growth; the hormone at this concentration caused an increase in dry weight by about 40 to 50% over the control, measured at a stationary phase. It was thus indicated that 2,4-D at $10^{-5}M$ provided the optimal condition for the suspension culture. Changes in pH of the medium were found to be affected by hormonal treatments during the first 2-3 days following the inoculation, after which the pattern of pH changes in hormone enriched media paralleled that of the control. Subsequent changes of $NH_4-N and NO_3-N$ from the medium by the cells, and also by growth of the cells. The uptake of $NH_4-N$ by the cells did not appear to be influenced by hormonal treatments. At a stationary phase, a considerable amout of $NO_3-N$ played a more important role than $NH_4-N$ in the growth of the carrot cell suspension culture.

• Journal of Plant Biology
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• 제33권4호
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• pp.259-269
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• 1990

Changes of peroxidase activity, polyamine content and ethylene production during somatic embryogenesis in suspension-cultured carrot (Daucus carota L.) cells were investigated. As compared with nonembyrogenic cells and their medium, embryogenic cells and their medium were characterized by higher levels of peroxidase at all times of culture period. Peroxidase in embryogenic cells showed higher oxidation activity of IAA than in nonembryogenic cells at the torpedo stage, but the IAA oxidation activity of peroxidase released into embryogenic medium was lower than that of peroxidase released into nonembryogenic medium. Peroxidase patterns of embryogenic and nonembryogenic cells showed three cathodic bands, and one anodic band, while peroxidase patterns released into embryogenic and nonembryogenic media did not show any anodic bands and the isoelectric points of cathodic peroxidase were pH 7.7, 7.5 and 6.6. Compared with nonembryogenic cells, polyamine content in embryogenic cells was increased by 15% at the torpedo stage, but polyamine ratio was constant, and ethylene production was extremely low at all times of culture period. Therefore, it is suggested that the peroxidase in embryogenic cells is correlated with embryogenesis by regulating hormone ratios through IAA oxidation, while the peroxidase isozyme patterns may be used as a biochemical marker of embryogenesis. The increase of polyamine content and the decrease of ethylene production suggest an interaction between polyamine and ethlyene during embryogenesis.

• 한국식생활문화학회지
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• 제33권1호
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• pp.55-61
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• 2018

This study examined the quality of bread made from a wheat flour, oat powder, and wild carrot powder mixture. The lightness and redness values decreased with increasing amount of wild carrot powder addition, while the yellowness was increased significantly. In a sample of wild carrot powder addition, the total volume was lower than the control. The texture profile analysis of oat bread, such as hardness, gumminess and cohesiveness decreased significantly with increasing amount of wild carrot powder addition. In the case of springiness, the value was not significant. The sensory evaluation of oat bread was significantly different in all analyses. Oat bread (BCB2.0) containing 2.0% (w/w) wild carrot powder showed the highest value in the four sensory evaluation items. Therefore, the 2.0% (w/w) addition of wild carrot powder addition was appropriate. An analysis of the physicochemical active component and DPPH scavenging activity of oat bread revealed a higher total flavonoid and total polyphenol content than normal bread. The DPPH scavenging activity was also 20.3% compared to the wild carrot powder. The availability of wild carrot powder in oat bread could be identified.

• 한국자원식물학회지
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• 제11권1호
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• pp.30-39
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• 1998

Carrot seedling emgryos showing variations in cotyledon number were selected and anatomical comparisons of the embryo vascular systems were performed between the variants and normal two cotyledonary (5) embryos from 800 seedings germinated . Externally, all of the four and six cotyledonary embryos had two cotyledonary petioles. Each of the cotyledonary petioles divided into two or three on the upper part fo the petiole which result in four and six cotyledons, respectively. However, the embryos had three different cotyledonary petioles in the three cotyledonary embryos. On the basis of the pattern of vascular system, the four and six cotyledonary embryos had the same basic vascular system as fnormal two cotyledonary embryos, Therefore the cotyledon number abnormality could result from the branching split of the abnormally thickened upper part of the cotyledonary petiole. However, the three cotyledonaryembryos had a different vascular system from the normla two cotyledonary embryos. They could be regarded as different varieties form the two cotyledon embryos. All embryos observed had short cylindrical plumule sheath which formed by the fusion of the cotyledon bases. The presence of plumule sheath strontgly implied that the initiation of the cotyledons was not from the two localized primordia but from the circular proimordiu formed at the blobular stageof embryo, and it is not consistent with current views of cotyledon initiation. On the formation of the primary vascular system of carrot seedlings, it is suggested that the primary vascular system of the plumule was formed independently from that of the root-hypocotyle-cotyledon system.

• 대한의생명과학회지
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• 제24권3호
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• pp.196-205
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• 2018

Reactive oxygen species (ROS) generated from metabolic reactions cause oxidative DNA damage, which results in oxidative tissue injury. Therefore, there is an increasing demand in the intake of high antioxidant sources in order to maintain a healthy environment in cells. In this study, we investigated the antioxidant and anti-inflammatory activities of Malus domestica (apple), Pyrus communis L. (pear), Daucus carota L. (carrot), Brassica oleracea var. (broccoli), Brassica oleracea var. capitata (cabbage), and Raphanus sativus L. (radish) obtained from the local market. Since these are common fruits and vegetables that are widely consumed, we aimed to investigate their beneficial properties, placing particular emphasis on their antioxidant and anti-inflammatory properties. The samples were processed via an indirect heating method and their properties were compared to their raw forms. Based on DPPH and ABTS assays, processed samples showed better antioxidant activities when compared to raw samples and processed pear samples exhibited the best antioxidant activity. The anti-inflammatory activities of the samples were also investigated in LPS-treated RAW 264.7 cells. mRNA expression of pro-inflammatory mediators and cytokines (iNOS, COX-2, $TNF-{\alpha}$, $IL-1{\beta}$, and IL-6) was assessed using RT-PCR. As expected, processed samples exhibited better iNOS inhibition when compared to their raw forms and processed broccoli and cabbage samples exhibited outstanding anti-inflammatory effects. The samples, up to 1 mg/mL concentration, did not exhibit cytotoxicity against RAW 264.7 cells as demonstrated by cell viability assays. Altogether, processed broccoli and cabbage samples exhibited the strongest anti-inflammatory properties.

• 유기물자원화
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• 제4권1호
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• pp.53-60
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• 1996

혐기적으로 1차 분해된 하수슬러지를 건물 중 기준으로 0, 1, 3, 그리고 5톤/10a 비율로 포장에 시용한 후, 대두 및 당근의 수량과 무기성분 흡수 등 몇가지 화학적 특성에 미치는 영향을 검토하였다. 슬러지 시용의 결과는 토양의 몇가지 화학적 특성을 증진시킨 반면, 작물에서의 Pb, Cd, Cr, As 및 Hg과 같은 유해 중금속의 흡수는 나타나지 않았다. 슬러지 시용은 작물의 N, P, K 및 Ca 등의 무기성분 흡수를 증가시켰고, 슬러지중 과량의 Al은 작물의 Al흡수에 별다른 영향을 주지 않았다. 또한 슬러지는 두 작물의 수량을 증가시킨 반면, 슬러지 미부숙으로 인해 당근의 외관적 품질을 저하시켰다. 결론적으로 하수슬러지의 작물 생육효과가 긍정적으로 평가된 반면, 농업적으로 이용할 때는 반드시 퇴비화와 같은 슬러지의 안정화가 선행되어야 한다.

• KSBB Journal
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• 제4권3호
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• pp.246-253
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• 1989

Agorobacterium rhizogenes에 의하여 유도된 당근(Daucus carota L. )의 hairy root를 배양하였으며 opine 유무에 대한 형질전환 확인 및 배지조성을 달리하여 성장률에 따른 색소함량을 비교하였고, 재분화된 식물체의 형태적 차이를 관찰하여 몇가지 결론을 얻었다. Hairy root는 균 접종 2-4주 후에 형성층 부위를 중심으로 유도되었다. 유도된 hairy root의 초기 배양에는 R.C.M 배지가 적합하였으며 MSO(2, 4-D $10^{-4} ml/ l, pH6, sucrose 5%, 질소원 0.03M 등)에서 최대 성장을 보여주었고 성장의 증가에 따른 색소의 형성은 비교적 안정하였다. 재분화된 식물체는 정상 식물체에 비하여 형태적으로 차이를 나타내었으며 형질전환된 hairy root 및 재분화된 식물체에서 mannopine 분석으로 Ri-plasmid에 의한 형질전환이 이루어졌음을 확인하였다.

• 한국식품영양학회지
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• 제34권1호
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• pp.114-122
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• 2021

The purpose of this study was to evaluate of black carrot on the antioxidant activity and quality characteristics of Sulgidduk. Sulgidduk was prepared with different amounts (0, 1, 2, 3, 4%) of black carrot. As the amount of added black carrot increased, the moisture content (p<0.05) and pH were decreased (p<0.001). Sugar content results showed the increase with the addition of black carrot (p<0.01). L (lightness) values and b (yellowness) values decreased whereas a (redness) values increased with an increased concentration of black carrot powder (p<0.001). The mechanical texture of Sulgidduk was decreased by the addition of black carrot considering hardness, chewiness and gumminess (p<0.001) while those of springiness, cohesiveness increased. Consumer acceptability test revealed that the 2% black carrot groups had a higher score than the other groups in respect to color, flavor, taste, texture, overall palatability. To examine antioxidant activities of Sulgidduk, total phenolic, DPPH radical scavenging activity, and total anthocyanin were tested. Total phenolic, DPPH radical scavenging activity, and total anthocyanin showed good vitality as amounts of black carrot powder increased (p<0.001). Based on the various aspects of results, 2% of black carrot added into Sulgidduk showed the best functionality and sensory qualities.

• 청정기술
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• 제21권1호
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• pp.39-44
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• 2015

본 연구에서는 다섯 종의 씨앗(춘채, 아욱, 양배추, 배추, 당근)을 이용하여 금속산화물 나노입자(CuO, NiO, Fe₂O₃, Co₃O₄, TiO₂, ZnO)들이 씨앗발아와 발아지수에 미치는 영향을 조사하였다. CuO, ZnO, NiO 나노입자는 씨앗 활성에 뚜렷한 독성 영향을 나타내었으며, 나노입자종류에 따라 상이한 민감도를 나타내었다. 각 나노입자에 대한 독성순서(EC₅₀ 범위)는 다음과 같다: CuO 6~27 mg/L > ZnO 16~86 mg/L > NiO 48~112 mg/L. 나머지 조사 대상 나노입자인 Co₃O₄, TiO₂, Fe₂O₃은 최대 1,000 mg/L 높은 농도 노출에도 뚜렷한 영향을 나타내지 않았다. 씨앗별 상이한 민감도를 나타내었으며, 가장 민감한 종인 아욱의 씨앗발아 EC₅₀은 CuO 5.5 mg/L ZnO 16.4 mg/L, NiO 53.4 mg/L로 조사되었다. 씨앗별 나노입자에 대한 독성 영향은 CuO > ZnO > NiO > Fe₂O₃ ≈ Co₃O₄ ≈ TiO₂ 나타났으나, 당근씨앗은 NiO [EC₅₀ 80.4(71.41~90.54) mg/L]와 ZnO [EC₅₀ 85.8(69.31~106.29) mg/L]가 유사한 독성을 나타내었다.

• Journal of Plant Biology
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• 제36권4호
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• pp.391-398
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• 1993

식물에서도 동물에서와 같이 RGD(Arg-Gly-Asp) sequence를 매개로 원형질막과 extracellular matrix(ECM)가 연결되어 있다는 사실이 알려지고 있으므로 RGD sequence가 식물세포의 생장, 분화 등의 여러 가지 생리적인 현상에 관여하리라는 가능성을 생각할 수 있다. 그래서 RGD sequence가 당근(Daucus carota L.) 배양세포에서 에틸렌 생성에 미치는 영향을 조사하였다. RGD sequence를 포함하는 합성 peptide는 당근 배양세포에서 에틸렌의 생성을 촉진시켰다. RGD peptide는 Indole-3-acetic acid(IAA)와 함께 처리한 경우 에틸렌 생성을 더욱 촉진시켰으며, ACC synthase의 활성도 증가시켰다. RGD sequence가 에틸렌 생성을 촉진시킬 때 어떠한 특이성이 있는가를 확인하기 위해 이와 유사한 RGE(Arg-Gly-Glu) peptide, RGK(Arg-Gly-Lys) peptide를 각각 처리하여 보았다. RGE peptide는 RGD peptide와 마찬가지로 에틸렌 생성을 촉진시켰으나 RGK peptide는 에틸렌 생성에 영향을 미치지 않았다. 따라서 RGD peptide의 에틸렌 생성 촉진작용은 RGD dequence를 포함하는 RGD peptide가 ACC synthase의 활성을 증가시켜서 일어나는 현상이라 생각되며, 또한 특정한 아미노산 서열을 요구하는 특이적 반응이라고 생각된다.