• Title/Summary/Keyword: Data De-Identification

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Identification of Candidate Transcripts Related to Drought Stress using Secondary Traits and qRT-PCR in Tropical Maize (Zea mays L.)

  • Kim, Hyo Chul;Song, Kitae;Moon, Jun-Cheol;Kim, Jae Yoon;Kim, Kyung-Hee;Lee, Byung-Moo
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.64 no.4
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    • pp.432-440
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    • 2019
  • Global climate change exerts adverse effects on maize production. Among abiotic stresses, drought stress during the tasseling stage (VT) can increase anthesis-silking intervals (ASI) and decrease yield. We performed an evaluation of ASI and yield using a drought-sensitive line (Ki3) and a drought-tolerant line (Ki11) to analyze the correlation with ASI and yield. Moreover, the de novo data of Ki11 were analyzed to find putative novel transcripts related todrought stress in tropical maize. A total of 182 transcripts, with a log2 ratio >1.5, were found by comparing drought conditions to a control. The top 40 transcripts of high expression levels in the de novo analysis were selected and analyzed with PCR. Of the 40 transcripts, six novel transcripts were detected by quantitative real-time PCR (qRT-PCR) using seedling and VT stage samples. Five transcripts (transcripts_1, 12, 34, 35, and 40) were up-regulated in the Ki11 shoot at seedling stage, and transcripts_1, 12, and 40 were up-regulated at the re-watering stage after 12 h of drought stress. The transcripts_32 and 34 were up-regulated at the VT stage. Hence, transcript_34 possibly plays a significant role in drought tolerance during the seedling and VT stages. The transcript_32 was identified as chloramphenicol acetyltransferase (CAT) by Pfam domain analysis. The function of the other transcripts remained unknown. Further characterization of these novel transcripts in genetic regulation will be of great value for the improvement of maize production.

Secondary Metabolites and Morphological Diversity in the Leaves of Perilla Landrace from Korea

  • Assefa, Awraris Derbie;Sung, Jung-Sook;Jeong, Yi-Jin;Lee, Ho-Sun;Rhee, Ju-Hee;Hur, On-Sook;Noh, Jae-Jong;Ro, Na-Young;Hwang, Ae-Jin;Lee, Jae-Eun
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2019.10a
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    • pp.64-64
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    • 2019
  • Screening and identification of genetic resources based on their phytoconstituents and morphological characters potentially provide baseline data for researchers, breeders, and nutraceutical companies who wish to formulate a nutrient-dense diet and health beneficial supplement. Thus, we evaluated the amount of total phenolic content and major phenolic compounds; examined if phenolic compounds could be used as distinguishing factors for perilla genetic resources; and investigated the relation between some quantitative and qualitative morphological characters with the contents of phenolic compounds in 360 accessions obtained from National Agrobiodiversity Center gene bank, Jeonju, Korea. Total phenolic content (TPC) was estimated using Folin-Ciocalteu colorimetric assay. Individual phenolic compounds were determined using an Ultra-High Performance Liquid Chromatography system equipped with Photodiode Array detector. Considerable variations were observed in TPC (7.99 to 117.47 mg GAE/g DE), rosmarinic acid (RA) (ND to 19.19 mg/g DE), caffeic acid (CA) (ND to 0.72 mg/g DE), apigenin-7-O-diglucuronide (ADG) (ND to 1.24 mg luteolin equivalent (LUE)/g DE), scutellarein-7-O-glucuronide (SG) (ND to 4.32 mg LUE/g DE), and apigenin-7-O-glucuronide (AG) (ND to 1.60 mg LUE/g DE). RA was the most dominant phenolic compound in most accessions (95.3%) followed by SG. The adaxial leaf color was light green, green and dark green in 13.8%, 65.0%, and 21.1 % of the accessions, respectively. 78.8% of the accessions had light green color at the abaxial side with the remaining being described as green. Most of the accessions (96.9%) were cordate shape, the remaining being eclipse. Intensities of green pigment at abaxial and adaxial leaf surfaces were correlated with contents of individual phenolic compounds and TPC whereas leaf length and width had no correlation with TPC, CA and RA, and negatively correlated with ADG, AG, and SG. Leaf shape was not related with content of phenolic compounds, color of leaves, or the length or width of leaves. Accessions IT57426, IT157434, IT267710, and IT267712 which contained relatively high contents of TPC and major phenolic compounds (RA and SG) could be used for further research in breeding and bioassay test. Our study result showed the contents of total phenolics and individual phenolic compounds along with the morphological characters could be useful distinguishing factors for perilla genetic resources.

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Implementation of RFID Reader System using the Data Encryption Standard Algorithm (표준 암호화 알고리즘을 이용한 RFID 판독 시스템의 구현)

  • 박성욱
    • Journal of Korea Society of Industrial Information Systems
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    • v.8 no.1
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    • pp.55-61
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    • 2003
  • The Data Encryption Standard(DES) has been a worldwide standard for over 20 years. DES is one of the block encryption techniques which ciphers 64-bit input data blocks using a 56-bit private key. The DES algorithm transforms 64-bit input in a series of steps into a 64-bit output. Thus, it is impossible to deduce the plaintext from the ciphertext which encrypted by this algorithm without the key. This paper presents an implementation of RFID roader system using the DES algorithm. An implemented system enhances the credibility of the encryption algorithm by using the Cipher Block Chining(CBC). Experimental results also show that the implemented system has better performance over the conventional commercial product.

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Damage assessment of beams from changes in natural frequencies using ant colony optimization

  • Majumdar, Aditi;De, Ambar;Maity, Damodar;Maiti, Dipak Kumar
    • Structural Engineering and Mechanics
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    • v.45 no.3
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    • pp.391-410
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    • 2013
  • A numerical method is presented here to detect and assess structural damages from changes in natural frequencies using Ant Colony Optimization (ACO) algorithm. It is possible to formulate the inverse problem in terms of optimization and then to utilize a solution technique employing ACO to assess the damage/damages of structures using natural frequencies. The laboratory tested data has been used to verify the proposed algorithm. The study indicates the potentiality of the developed code to solve a wide range of inverse identification problems in a systematic manner. The developed code is used to assess damages of beam like structures using a first few natural frequencies. The outcomes of the simulated results show that the developed method can detect and estimate the amount of damages with satisfactory precision.

Strategic Analysis Evolution: Scenario Planning and Simulation Based on The Methodology of System Dynamics

  • Bassi, Andrea M
    • Korean System Dynamics Review
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    • v.5 no.2
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    • pp.199-216
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    • 2004
  • The present study is aimed at developing the optimal instruments for dispelling the uncertainty factors during the formulation of strategies for corporate development. The objective is the creation of a complete model of strategic analysis, which encompasses both the environment (internal and external) and the management rational component. This model -built on the analysis of three corporate cases - is concretized by a simulation for testing the strategy by the means of software which enables the users to cope with a dynamic and complex corporate environment. The research questions regard the development of a complete strategic analysis, which covers the entire decision-making process; the concrete assessment of the business strategy on the basis of quantitative data: the identification and enhancement of the critical variables of business administration, in such a complex and dynamic reality as the corporate environment.

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High-level Expression, Polyclonal Antibody Preparation and Bioinformatics Analysis of Bombyx mori Nucleopolyhedrovirus orf47 Encodes Protein

  • Wu, Chao;Guo, Zhongjian;Chen, Keping;Shen, Hongxing
    • International Journal of Industrial Entomology and Biomaterials
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    • v.16 no.2
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    • pp.87-92
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    • 2008
  • Bombyx mori nucleopolyhedrovirus (BmNPV) orf47 gene was characterized for the first time. The coding sequence of Bm47 was amplified and subcloned into the prokaryotic expression vector pET-30a(+) in order to produce His-tagged fusion protein in the BL21 (DE3) cells. The His-Bm47 fusion protein was expressed efficiently after induction with IPTG. The purified fusion protein was used to immunize New Zealand white rabbits to prepare polyclonal antibody. As the genome of BmNPV is available in GenBank and the EST database of BmNPV is expanding, identification of novel genes of BmNPV was conceivable by data-mining techniques and bioinformatics tools. Structural bioinformatics approach to analyze the properties of Bm47 encodes protein.

Raising Risk and Suggesting Solution about Personal Information De-identification in Big-Data Environment (빅데이터 환경에서 개인정보 비식별화에 대한 위험성 제기 및 대응 방안 제시)

  • Lee, Su-Rim;Jang, Woong-Tae;Bae, Jae-Young;Lee, Chan-Ho;Hyun, Beom-Su
    • Proceedings of the Korea Information Processing Society Conference
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    • 2016.10a
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    • pp.297-300
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    • 2016
  • 최근 빅데이터 산업이 발전하고 있는 상황에서 빅데이터 산업에 활용되는 개인정보의 보호에 관한 문제가 대두하고 있다. 빅데이터 산업에서 개인정보를 활용하기 위해서는 비식별화 조치를 해야 한다. 하지만 비식별화는 비식별화 평가 모델 자체의 취약성과 더불어 비식별화된 개인정보를 재식별화 하는 위험성도 존재한다. 본 논문은 적정성 평가 모델, 비식별화 조치 기술, 재식별에 관한 위험성을 연구하고 각 위험성에 대한 대응 방안을 통해 재식별화의 문제를 해결하여 빅데이터 산업에서 비식별화된 개인정보가 안전히 쓰일 수 있도록 해야 한다.

Elucidating H/D-Exchange Mechanism of Active Hydrogen in Aniline and Benzene-1,2-dithiol

  • Ahmed, Arif;Islam, Syful;Kim, Sunghwan
    • Mass Spectrometry Letters
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    • v.12 no.4
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    • pp.146-151
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    • 2021
  • In this study, the hydrogen/deuterium (HDX) exchange mechanism of active hydrogen, nitrogen, and sulfur-containing polycyclic aromatic hydrocarbon (PAH) dissolved in toluene and deuterated methanol by atmospheric pressure photoionization (APPI) is investigated. The comparison of the data obtained using APPI suggests that aniline and benzene-1,2-dithiol contain two exchanging hydrogens. The APPI HDX that best explains the experimental findings was investigated with the use of quantum mechanical calculations. The HDX mechanism is composed of a two-step reaction: in the first step, analyte radical ion gets deuterated, and in the second step, the hydrogen transfer occurs from deuterated analyte to de-deuterated methanol to complete the exchange reaction. The suggested mechanism provides fundamentals for the HDX technique that is important for structural identification with mass spectrometry. This paper is dedicated to Professor Seung Koo Shin for his outstanding contributions in chemistry and mass spectrometry.

The Smart Port Management System Based on Big-data (빅데이터 기반 스마트 항만 운용시스템)

  • Lee, Woo;Kim, Sang-Hyun;Oh, Seung-Hong;Kim, Won-Jung
    • The Journal of the Korea institute of electronic communication sciences
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    • v.17 no.1
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    • pp.167-172
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    • 2022
  • Currently, ship control, tug, and pilot work in import/export ports including Gwangyang Port are operated according to factors such as the order of arrival and departure regardless of the shipping company. Also, even this is done very inefficiently by hand. Therefore, there is an urgent need to develop a system to increase the efficiency of port and ship operation through standardization and digitalization of tasks related to Berthing and unberthing of ships. In this study, we propose a method to increase the efficiency of port and vessel operation by designing a smart port operation system based on big data such as vessel location information, pilotage and tug schedule, arrival/departure operation information, and weather information.

Research on technical protection measures through risk analysis of pseudonym information for life-cycle (가명정보 Life-Cycle에 대한 위험 분석을 통한 관리적/기술적 보호조치 방안에 대한 연구)

  • Cha, Gun-Sang
    • Convergence Security Journal
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    • v.20 no.5
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    • pp.53-63
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    • 2020
  • In accordance with the revision of the Data 3 Act, such as the Personal Information Protection Act, it is possible to process pseudonym information without the consent of the information subject for statistical creation, scientific research, and preservation of public records, and unlike personal information, it is legal for personal information leakage notification and personal information destruction There are exceptions. It is necessary to revise the pseudonym information in that the standard for the pseudonym processing differs by country and the identification guidelines and anonymization are identified in the guidelines for non-identification of personal information in Korea. In this paper, we focus on the use of personal information in accordance with the 4th Industrial Revolution, examine the concept of pseudonym information for safe use of newly introduced pseudonym information, and generate / use / provide / destroy domestic and foreign non-identification measures standards and pseudonym information. At this stage, through the review of the main contents of the law or the enforcement ordinance (draft), I would like to make suggestions on future management / technical protection measures.