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Cloning of hadA-like Sigma Factor Gene from Streptomyces coelicolor A3(2) (Streptomyces coelicolor A3(2)에서 hrdA유사 Sigma 인자 유전자의 클로닝)

  • Hahn, Ji-Sook;Cho, Eun-Jung;Roe, Jung-Hye
    • Korean Journal of Microbiology
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    • v.32 no.4
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    • pp.264-270
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    • 1994
  • A gene coding for a novel putative $\sigma$ factor of RNA polymerase has been identified from Streptomyces coelicolor A3(2) using Escherichia coli rpoS gene fragment as a probe. The 486 bp rpoS gene fragment was amplified from E. coli genomic DNA by PCR with two synthetic oligonucleotides, the sequences of which were deduced from the amino acid sequences in the regions 2.3 and 4.2 conserved among various bacterial factors. When E. coli genomic DNA fragments were hybridized with cloned rpoS probe, only one band corresponding to rpoS gene (3.2 kb PvuII fragment or 2.3 kb KpnI fragment) was detected. In S. coelicolor, however, two bands were detected both in PvuII digested DNA and SalI digested DNA. 3.5 kb PvuII fragment which binds the rpoS gene probe was cloned (pMS1) from the sublibrary, and the nucleotide sequences of 1.0 kb BamH'/HincII subclone (pBH2) was partially determined. The nucleotide sequences revealed extensive similarity to other $\sigma$ factor genes of S. coelicolor (hrdA, hrdB, hrdC, hrdD), S. aureofaciens (hrdA, hrdB, hrdC, hrdD), Synechococcus species, Pseudomonas aeruginosa, Stigmatella aurantiaca, and Anabaena species. The nucleotide sequences in regions 1.2 and 4 were compared with the corresponding regions of 5 known ${\sigma}$ factor genes of S. coelicolor by multiple alignment. It turned out that the cloned gene is most closely related to hrdA showing 88% amino acid similarity in region 1.2 and 75% in region 4.

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The Impact of Industrial Diversity to Unemployment and Employment Instability: An Analysis of Regional Economy Using Panel Regression Model (산업구조의 다양성이 실업과 고용불안정에 미치는 영향: 패널회귀모형을 이용한 지역경제 분석)

  • Ryu, Suyeol;Choi, Ki-Hong;Ko, Seung-Hwan;Yoon, Seong-Min
    • Journal of the Economic Geographical Society of Korea
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    • v.17 no.1
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    • pp.129-146
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    • 2014
  • This paper investigates how industrial diversity affects unemployment and employment instability from the perspective of the regional economy. Through this analysis, we examine how the industry-specific policy to promote some industry strategically in most of areas affects the stability of the regional economy. We measure Herfindahl indexes using the 1993-2010 data of 16 regions in Korea, and use panel regression model for empirical analysis. The main results from this empirical analysis are summarized as follows. First, we confirm that the industrial structure of most regions has been changed to the direction of specialization in 1990s and to the direction of diversification in 2000s through analyzing the changes in the values of Herfindahl indexes during the given period. Second, we find from the estimation results of panel regression model that the higher industrial diversity in most of regions is, the lower the unemployment rate is. However, a statistically significant relationship between industrial diversity and employment instability only partially confirmed. Third, there exist high unemployment rate and employment instability in most metropolitan areas, but it is hard to say that this relationship is highly statistically significant. From the results of the empirical analysis, it is likely that the industry-specific policies such as the regional strategic industry development policies unlike policy goals make the unemployment rate to rise and economic instability to increase. From the viewpoint of employment aspects, the strategies to increase industrial diversity would be desirable rather than those to specialize in the industrial structure.

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Material Characteristic of Slags and Iron Bloom Produced by Smelting Process Using Sand Iron (사철 제련을 통해 생산된 슬래그와 괴련철의 재료과학적 특성 비교)

  • Cho, Sung Mo;Cho, Hyun Kyung;Kwon, In Cheol;Cho, Nam Chul
    • Journal of Conservation Science
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    • v.34 no.1
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    • pp.39-50
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    • 2018
  • This study replicated traditional smelting methods to produce iron blooms from sand iron. The metallurgical properties of the slag and the iron blooms were analyzed. The sand iron materials used in the smelting experiments, which were based on ancient documents, were collected from Gyeong-Ju and Pohang. Analysis by WD-XRF and XRD showed that Gyeong-Ju's sand iron contains a high-titanium, with magnetite, and Pohang's sand iron contains a low-titanium, which magnetite and ilmenite were mixed. Analysis of the slag with XRD, and the micro-structure with metal microscopes and SEM-EDS, confirmed that the major compounds in the slag of the Gyeong-Ju's sand iron were fayalite and $w\ddot{u}stite$, and those in the slag of the Pohang's sand iron were titanomagnetite and fayalite. The differences in the main constituents were confirmed according to the Ti quantity. Finally, we observed the microstructures of the iron blooms. In the case of the iron bloom produced from Gyeong-Ju's sand iron, the outside was found to be dominantly a pearlite of eutectoid steel, while the inside was a hypo-eutectoid steel where ferrite and pearlite were mixed together. While, the major component of the iron bloom produced from Pohang's sand iron was ferrite, which is almost like pure iron. However, there were many impurities inside the iron blooms. Therefore, this experiment confirmed that making ironware required a process that involved removing internal impurities, refining, and welding. It will be an important data to identify the characteristics of iron by-products and the site through traditional iron-making experiments under various conditions.

Impact Analysis of Tributaries and Simulation of Water Pollution Accident Scenarios in the Water Source Section of Han River Using 3-D Hydrodynamic Model (3차원 수리모델을 이용한 한강 상수원구간 지류영향 분석 및 수질오염사고 시나리오 모의)

  • Kim, Eunjung;Park, Changmin;Na, Mijeong;Park, Hyeon;Kim, Bogsoon
    • Journal of Korean Society on Water Environment
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    • v.34 no.4
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    • pp.363-374
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    • 2018
  • The Han River serves as an important water resource for the city of Seoul, Korea and in the neighboring metropolitan areas. From the Paldang dam to the Jamsil submerged weir, the 4 water intake stations that are located for the Seoul metropolitan population were under review in this study. Therefore the water quality management in this section is very important to monitor, analyze and review to rule out any safety concerns. In this study, a 3-D hydrodynamic model, EFDC (Environmental Fluid Dynamics Code), was applied to the downstream of the Paldang Dam in the Han River, which is about 23 km in length, to determine issues related to water resource management. The 3-D grid was composed of 2,168 horizontal grids and three vertical layers. In this case, the hydrodynamic model was calibrated and verified with an observed average daily water surface elevation, water temperature and flow rate data for 3 years (2013~2015). The developed EFDC model proved to reproduce the hydrodynamics of the Han River well. The composition ratios of the noted incoming flows at the monitored intake stations for 3 years and their flow patterns in the river were analyzed using the validated model. It was found that the flow of the Wangsuk Stream depended on the Paldnag dam discharge, and it was noted that the composition ratios of the stream at the intake stations changed accordingly. In a word, the Wangsuk Stream moved mainly along the right bank of the Han River under the condition of a normal dam flow. As can be seen, when the dam discharge rate was low, the incidence of lateral mixing was often seen. The scenario analyses were also conducted to predict the transport of conservative pollutants as in the case of a chemical spill accident. Generally speaking, when scenarios were applied, the arrival time and concentration of pollutants at each intake station was thus predicted.

The Robust Phylogeny of Korean Wild Boar (Sus scrofa coreanus) Using Partial D-Loop Sequence of mtDNA

  • Cho, In-Cheol;Han, Sang-Hyun;Fang, Meiying;Lee, Sung-Soo;Ko, Moon-Suck;Lee, Hang;Lim, Hyun-Tae;Yoo, Chae-Kyoung;Lee, Jun-Heon;Jeon, Jin-Tae
    • Molecules and Cells
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    • v.28 no.5
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    • pp.423-430
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    • 2009
  • In order to elucidate the precise phylogenetic relationships of Korean wild boar (Sus scrofa coreanus), a partial mtDNA D-loop region (1,274 bp, NC_000845 nucleotide positions 16576-1236) was sequenced among 56 Korean wild boars. In total, 25 haplotypes were identified and classified into four distinct subgroups (K1 to K4) based on Bayesian phylogenetic analysis using Markov chain Monte Carlo methods. An extended analysis, adding 139 wild boars sampled worldwide, confirmed that Korean wild boars clearly belong to the Asian wild boar cluster. Unexpectedly, the Myanmarese/Thai wild boar population was detected on the same branch as Korean wild boar subgroups K3 and K4. A parsimonious median-joining network analysis including all Asian wild boar haplotypes again revealed four maternal lineages of Korean wild boars, which corresponded to the four Korean wild boar subgroups identified previously. In an additional analysis, we supplemented the Asian wild boar network with 34 Korean and Chinese domestic pig haplotypes. We found only one haplotype, C31, that was shared by Chinese wild, Chinese domestic and Korean domestic pigs. In contrast to our expectation that Korean wild boars contributed to the gene pool of Korean native pigs, these data clearly suggest that Korean native pigs would be introduced from China after domestication from Chinese wild boars.

A Comparative Analysis of Ensemble Learning-Based Classification Models for Explainable Term Deposit Subscription Forecasting (설명 가능한 정기예금 가입 여부 예측을 위한 앙상블 학습 기반 분류 모델들의 비교 분석)

  • Shin, Zian;Moon, Jihoon;Rho, Seungmin
    • The Journal of Society for e-Business Studies
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    • v.26 no.3
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    • pp.97-117
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    • 2021
  • Predicting term deposit subscriptions is one of representative financial marketing in banks, and banks can build a prediction model using various customer information. In order to improve the classification accuracy for term deposit subscriptions, many studies have been conducted based on machine learning techniques. However, even if these models can achieve satisfactory performance, utilizing them is not an easy task in the industry when their decision-making process is not adequately explained. To address this issue, this paper proposes an explainable scheme for term deposit subscription forecasting. For this, we first construct several classification models using decision tree-based ensemble learning methods, which yield excellent performance in tabular data, such as random forest, gradient boosting machine (GBM), extreme gradient boosting (XGB), and light gradient boosting machine (LightGBM). We then analyze their classification performance in depth through 10-fold cross-validation. After that, we provide the rationale for interpreting the influence of customer information and the decision-making process by applying Shapley additive explanation (SHAP), an explainable artificial intelligence technique, to the best classification model. To verify the practicality and validity of our scheme, experiments were conducted with the bank marketing dataset provided by Kaggle; we applied the SHAP to the GBM and LightGBM models, respectively, according to different dataset configurations and then performed their analysis and visualization for explainable term deposit subscriptions.

Natural Enemies of the Asian Gypsy Moth, Lymantria dispar asiatica (Lepidoptera: Erebidae) and the Genetic Variation Analysis of L. dispar Multiple Nucleopolyhedrovirus (국내 매미나방(나비목: 태극나방과) 천적 및 매미나방 핵다각체병바이러스의 유전적 다양성 조사)

  • Hwang, Hwal-Su;Lee, Young Su;Lee, Hee A;Choi, Duck Soo;Lee, Kyeong-Yeoll
    • Korean journal of applied entomology
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    • v.60 no.4
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    • pp.379-386
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    • 2021
  • Asian gypsy moth, Lymantria dispar asiatica (Lepidoptera: Erebidae), which is an indigenous pest in Korea, a large outbreak has been reported in Gyeonggi-do, Chungcheong-do, and northern Gyeongsangbuk-do from 2019 in forest and nearby downtown areas, causing emotional damage to forests and city dwellers. During the indoor culture of gypsy moth eggs collected in Yecheon, Gyeongbuk in 2021 we found that 79.65% (321/403 of first instar larvae) were died due to Lymantria dispar multiple nucleopolyhedrovirus (LdMNPV) infection. Sequence analyses of 36 gypsy moths collected from 12 regions in Korea using LdMNPV late expression factor-8 (lef-8) and polyhedrin (polh) genes showed a genetic variation of 0.80% and 0.86%, respectively. Comparison to GenBank data showed that the Korean samples were most similar to LdMNPV in Japan, whereas most different to those of Turkey. These results showed a high infection rate of LdMNPV in Korea and LdMNPV is one of the important population regulators of the gypsy moth.

A study on the effect of accounting information on dividend policy by measuring corporate conservatism (From the perspective of the internal accounting management system) (기업보수주의 측정으로 회계정보가 배당정책에 미치는 연구 (내부회계 관리제도 관점에서))

  • Lee, Soon Mi;You, Yen Yoo
    • Journal of Digital Convergence
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    • v.19 no.8
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    • pp.141-149
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    • 2021
  • This study investigated the effect of accounting information on dividend policy as a measure of corporate conservatism from the perspective of the internal accounting management system. The verification is based on a sample of 543 companies listed on securities (excluding KOSDAQ and financial industry) among the Bank of Korea (2019) 「2018 Corporate Management Analysis」 and company analysis of the Korea Productivity Center (financial data disclosed as listed companies as a December settlement company) was composed. Using SPSS 22, empirical analysis was conducted using exploratory factor analysis and regression analysis. The first is the verification related to corporate conservatism and the role of dividend policy, and it is verification of whether internal accounting management influences financial decision-making. Second, if internal accounting management exists, it is a verification of how conservatism and investment policies (in-house reserve, debt borrowing, capital increase, dividends, etc.) affect the corporate value according to accounting information. As a result, from the perspective of the internal accounting management system, it was found that among the variables of accounting information, profitability can have a positive effect on corporate conservatism and dividend policy as a corporate valuation method of reinvestment. In addition, it has been proven that corporate conservatism has an effect on profitability-to-value through capital accumulation and reinvestment such as surplus and internal reserves. In the future, we will study and discuss the complementarity of corporate conservatism and dividend policy in relation to governance structure and improvement of the internal accounting management system.

A Study on the Analysis of Management Characteristics of Coastal Port Freight Transportation Business Using Panel Regression Analysis (패널회귀분석을 이용한 내항 화물운송사업체의 경영특성 분석에 관한 연구)

  • Kim, Suk;Park, Sung-Hoon;Yang, Tae-Hyeon;Yeo, Gi-Tae
    • Journal of Digital Convergence
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    • v.17 no.3
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    • pp.79-92
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    • 2019
  • This study analyzes the effects of freight transportation income, capital, asset, non-operating expenses, and debt ratio on the debts of inner port freight transportation businesses through the GLS of panel regression analysis and the estimation of fixed effects model. The factors and hypotheses were established through a theoretical background review, and the financial statement and profit and loss data of inner port freight transportation businesses for 10 years from 2006 to 2015 were analyzed. The results showed that assets had positive effects on debts, and negative effects on capital, non-operating expenses, and debt ratio, but no effect on freight transportation income. This result empirically demonstrates the tendency of inner port freight transportation businesses to secure assets by increasing debts, creation of debt reduction leverage effect using non-operating expenses such as interest expenses through bank borrowing, and the adoption of management characteristics and financial operation method to lower the debt ratio by reducing capital more than debts. In future studies, it is necessary to analyze coastal port freight transportation business by industry (oil tankers, cargo ships, and barge ships), and regions such as East, West and South sea.

Warm Temperate species extract Analysis for Jeollanamdo Forest Resources Institute extracts Bank Data building (전라남도산림자원연구소 추출물은행 데이터 구축을 위한 난대수종의 추출물 분석 연구)

  • Kim, Hyun-Woo;Jo, Geon-Ung;Yeo, Hye-jeong;Kim, Hyeon-Seok;Eo, JI-Hyun;Beak, Hyo-Eun;Park, Jong-Seok;Oh, Chan-Jin;Oh, Deuk-sil;Park, Hwa-Sik
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2019.04a
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    • pp.101-101
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    • 2019
  • 나고야 의정서가 국내에 본격 시행됨에 따라 전남산림자원연구소는 도내 유용 산림 자원의 주권 확립 및 자원 정보의 DB 구축을 위하여 산림생명자원 추출물은행을 운영 중에 있다. 그 일환으로 1차년 난대 수종 4종(동백, 비자, 편백, 황칠)의 부위(잎, 가지) 시기(1차:4월, 2차:7월, 3차:9월, 4차:11월) 용매별(열수, 에탄올) 추출물을 제작하여, 유효성분 및 생리활성의 분석을 실시하였다. 총 폴리페놀(Total Polyphenol) 함량을 150 ug/g 이상을 기준으로 분석 분류한 결과, 동백 잎(4차) 열수, 가지(1, 3차), 잎(4차) 에탄올, 비자 잎(3, 4차) 열수, 가지(2, 3차), 잎(1, 3, 4차) 에탄올, 편백 가지(3차) 열수, 잎(3차), 가지(2, 3차) 에탄올, 황칠 잎(2, 3, 4차) 에탄올 추출물에서 높은 함량의 총 폴리페놀을 확인 할 수 있었다. 다음으로, 프리라디칼(DPPH) 소거능을 측정하여 50 % 이상을 기준으로 분류한 결과, 동백(1~4차), 가지(1~3차) 열수, 잎(1~4차), 가지(1, 3, 4차) 에탄올, 비자 잎(3차) 열수, 잎(2, 4차) 에탄올, 편백 잎(3, 4차), 가지(1차) 열수, 잎(1~3차), 가지(1, 3, 4차) 에탄올, 황칠 잎(2~4차) 열수 추출물에서 높은 프리라디칼(DPPH) 소거능을 보여 주었다. 이와 같이, 수종별로 부위 시기 용매별 추출물에 따른 유효성분과 생리활성에 차이를 확인 할 수 있었다. 이를 바탕으로 산업화 활용 시 채취 시기 및 부위를 특정 하는 자료로 도움 될 것으로 생각되며, 지속적으로 연계 추출 및 분석을 통해 보다 유의성 있는 자료 수집을 계속 진행 중 입니다. 이러한 산림생명자원 추출물은행의 연구 결과를 바탕으로 보다 심도 있게 반복적인 데이터를 수집 구축하고, 연구개발에 활용한다면, 고부가가치 임산 자원을 개발 및 산업화에 큰 도움이 되고 임업임 소득증대에 기여할 것이다.

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