• Journal of Microbiology and Biotechnology
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• v.14 no.1
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• pp.110-115
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• 2004

This study was undertaken to develop PCR primers for the simultaneous detection of Fusobacterium nucleatum and Actinobacillus actinomycetemcomitans, using two species-specific reverse primers in combination with a single conserved forward primer. These primers target the variable and conserved regions of the 16S rDNA. The primer specificity was tested against (i) four F. nucleatum and three A. actinomycetemcomitans strains and (ii) seven representatives of the different species of oral bacteria. The primer sensitivity was determined by testing serial dilutions of the purified genomic DNA of F. nucleatum and A. actinomycetemcomitans. The data indicate that species-specific amplicons could be obtained for all the F. nucleatum and A. actinomycetemcomitans strains tested, which were not found in the seven other species. The multiplex PCR could detect as little as 4 fg of chromosomal DNA of F. nucleatum and A. actinomycetemcomitans simultaneously. These findings suggest that these PCR primers are highly sensitive and are suitable for applications in epidemiological studies, diagnosis, and monitoring F. nucleatum and A. actinomycetemcomitans after the treatment of periodontitis.

• The Korean Journal of Applied Statistics
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• v.33 no.3
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• pp.321-334
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• 2020

The US Federal Reserve (Fed) has decided to cut interest rates. When we look at the expression of the FOMC statements at the time of policy change period we can understand that Fed has been communicating with markets through a change of word selection. However, there is a criticism that the method of analyzing the expression of the decision sentence through the context can be subjective and limited in qualitative analysis. In this paper, we evaluate the signaling effect of FOMC statements based on previous research. We analyze decision making characteristics from the viewpoint of text mining and try to predict future policy trend changes by capturing changes in expressions between statements. For this purpose, a decision tree and neural network models are used. As a result of the analysis, it can be judged that the discrepancy indicators between statements could be used to predict the policy change in the future and that the US Federal Reserve has systematically implemented policy signaling through the policy statements.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.17 no.4
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• pp.222-230
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• 2016

HEMIRE is a 6,000-meter-class remotely operated vehicle (ROV) that has been developed for observation and sampling of objects of interest on the deep seabed. We first carried out deep-seabed exploration around the slopes of the Hupo Bank and the Ulleung Basin in the East Sea in June 2015. Over two weeks, a total of 10 dives were made from a support ship, the R/V Onnuri, at eight stations with water depth ranging between 194 and 2,080 m. The dive times ranged from 1 to 6 hours, depending on the operating conditions. We obtained the following results: 1) video images of the deep seafloor; 2) red snow crab density data (a major fishery resource) and inventories of deep-sea fauna, including an unrecorded organism; 3) specific topographies such as canyons slopes; 4) an undisturbed sediment core obtained using a push corer; and 5) observations of the seabed surface covered with discarded anthropogenic waste material.

• Journal of Korean Society for Geospatial Information Science
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• v.18 no.2
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• pp.29-33
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• 2010

The purpose of LiDAR ground classification is to archive both goals which are acquiring confident ground points with high precision and describing ground shape in detail. In spite of many studies about developing optimized algorithms to kick out this, it is very difficult to classify ground points and describing ground shape by airborne LiDAR data. Especially it is more difficult in a dense forested area like Korea. Principle misclassification was mainly caused by complex forest canopy hierarchy in Korea and relatively coarse LiDAR points density for ground classification. Unfortunately, a lot of LiDAR surveying performed in summer in South Korea. And by that reason, schematic LiDAR points distribution is very different from those of Europe. So, this study propose enhanced ground classification method considering Korean land cover characteristics. Firstly, this study designate highly confident candidated LiDAR points as a first ground points which is acquired by using big roller classification algorithm. Secondly, this study applied weighted gradient kernel(WGK) algorithm to find and include highly expected ground points from the remained candidate points. This study methods is very useful for reconstruct deformed terrain due to misclassification results by detecting and include important terrain model key points for describing ground shape at site. Especially in the case of deformed bank side of river area, this study showed highly enhanced classification and reconstruction results by using WGK algorithm.

• Journal of Service Research and Studies
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• v.10 no.1
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• pp.17-31
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• 2020

Open banking, the global trend of the financial industry, is the driving force behind various innovations in the financial market in the future. The right policy direction and detailed tasks are important for triggering the differentiation and reunion of the financial industry. The purpose of this study is to investigate and analyze the background of open banking, domestic and international trends, and Korea's open banking policy. The policy directions and tasks for successful settlement and activation of open banking system are carefully suggested. Open banking is a policy to allow third party provider(TPP) access to bank accounts and open payment functions under the explicit consent of the customer. The opening of the open banking era is expected to begin competition and cooperation between banks and fintech companies in earnest, thus enhancing the competitiveness of the financial industry and contributing to the utility of financial consumers. To this end, policymakers should make every effort to advance open-ended financial settlement infrastructure, open banking legal grounds, and minimize side effects such as customer data leakage and poor financial system stability. Banks and fintech companies will need to focus on scattered customer financial information on a single platform and develop it into a convergence and discrimination of true financial services.

• Korean Journal of Environment and Ecology
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• v.18 no.1
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• pp.61-74
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• 2004

The study was to investigate and compare plants and frequency of appearing species of vegetation transect in a straight and a restoration Section of Bulgwang stream in Seoul. Some part of Bulgwang stream was restored as a closed-to-nature stream. This present study consists of two investigations, the first investigation being conducted on May and the second one on August -September 2003. Based on the analysis of the data collected from both investigations, this study classified and listed all of the plants appeared in a straight and a restoration section of the stream. The study used the Belt-transect method in order to examine and analyze discover the appearance frequency of the vegetaton transect per 200 meters. During the period of the first investigation and the second one, plants of 33 families, 73 classes, and 88 species appeared in the straight section, while plants of 36 families, 76 classes, and 90 species appeared in the restoration section. The Naturalized Index of the straight section was 22.7％, and that of the restoration section was 21.1％. As shown on the results indicating that the Urbanization Index of the straight section was 7.8％, and that of the restoration section was 7.4％, the Urbanization Index of the straight section was slightly higher than the restoration section. The results using belt-transect analysis method showed that in case of the straight section, the plants of the highest appearance frequency on the riverside were Persicaria hydropiper and Humulus japonicus. On the contrary, in the case of restoration section, the plants showing a higher appearance frequency on the riverside were identified as Salix gracilistyla, Persicaria hydropiper, Rumex crispus, and Humulus japonicus. In conclusion, there was no difference between the straight and the restoration section in the bank with regard to vegetation transect. However, a remarkable difference was found on the riverside for the Salix gracilistyla which was introduced in the restoration section.

• Journal of Life Science
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• v.30 no.11
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• pp.1012-1020
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• 2020

Remicade is a therapeutic biosimilar natural antibody in which the mouse variable domain has been linked to the human constant domain. It is a chimeric monoclonal antibody specific to tumor necrosis factor-alpha (TNF-α) and has been developed for the treatment of rheumatoid arthritis. To investigate the biological activity of the Remicade antibody, we carried out a bioinformatics study using a protein data bank to characterize the TNF-α antigen binding mechanism of the Remicade natural antibody. Because the production of the Remicade antibody is often limited by genetic instability of the natural antibody-producing cell, we generated a Remicade single-chain variable domain fragment antibody (Remicade) in which a heavy chain variable domain (VH) is joined with a light chain variable domain (VL) by a polypeptide linker. Furthermore, Remicade was fused to a leucine zipper (RemicadeScZip) for higher production and higher antigen-binding activity than Remicade. The Remicade and Remicade ScZip were expressed in Escherichia coli and purified by a Ni⁺-NTA-agarose column. As expected, the purified proteins had migrated as 28.80 kDa and 33.96 kDa in sodium dodecyl sulfate-polyacrylamide electrophoresis. The TNF-α antigen binding activity of Remicade was not observed by ELISA and western blot. In contrast, RemicadeScZip showed antigen-binding activity. Additional bio-layer interferometry analysis confirmed the antigen-binding activity of RemicadeScZip, suggesting that the leucine zipper stabilized the folding of RemicadeScZip in a denatured condition and improved the TNF-α antigenbinding activity.

• Korean Security Journal
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• no.52
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• pp.11-40
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• 2017

This study analyzed individual-level and country-level factors affecting justification of domestic violence amid emerging social significance of family violence. For individual-level variables, prejudice against women in economic and social roles were used from the World Value Survey data. As for country-level variables, total of 36 countries were analyzed with indices that represents gender equality such as women's employment rate and democracy index. Women's employment rate was gathered from the Labour Market Database of the World Bank and democracy index was from the Economist Intelligence Unit. Results showed that both individual-level, prejudice against women in economic and social roles and country-level variables such as women's employment rate and democracy index had significant effects on justification of domestic violence. This result implies the importance of creating positive social culture which promotes positive attitudes towards perceptions of gender role and gender equality. As well, country-level endeavors to raise gender equality in employment deem important. Based on these findings, policy implications and recommendations for future research were discussed.

• Journal of Ginseng Research
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• v.35 no.1
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• pp.31-38
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• 2011

In order to distinguish the cultivation area of Panax ginseng, principal component analysis (PCA) using quantitative and qualitative data acquired from HPLC was carried out. A new HPLC method coupled with evaporative light scattering detection (HPLC-ELSD) was developed for the simultaneous quantification of ten major ginsenosides, namely $Rh_1$, $Rg_2$, $Rg_3$, $Rg_1$, Rf, Re, Rd, $Rb_2$, Rc, and $Rb_1$ in the root of P. ginseng C. A. Meyer. Simultaneous separations of these ten ginsenosides were achieved on a carbohydrate analytical column. The mobile phase consisted of acetonitrile-water-isopropanol, and acetonitrile-water-isopropanol using a gradient elution. Distinct differences in qualitative and quantitative characteristics for ginsenosides were found between the ginseng roots produced in two different Korean cultivation areas, Ganghwa and Punggi. The ginsenoside profiles obtained via HPLC analysis were subjected to PCA. PCA score plots using two principal components (PCs) showed good separation for the ginseng roots cultivated in Ganghwa and Punggi. PC1 influenced the separation, capturing 43.6% of the variance, while PC2 affected differentiation, explaining 18.0% of the variance. The highest contribution components were ginsenoside $Rg_3$ for PC1 and ginsenoside Rf for PC2. Particularly, the PCA score plot for the small ginseng roots of six-year old, each of which was light than 147 g fresh weight, showed more distinct discrimination. PC1 influenced the separation between different sample sets, capturing 51.8% of the variance, while PC2 affected differentiation, also explaining 28.0% of the variance. The highest contribution component was ginsenoside Rf for PC1 and ginsenoside $Rg_2$ for PC2. In conclusion, the HPLC-ELSD method using a carbohydrate column allowed for the simultaneous quantification of ten major ginsenosides, and PCA analysis of the ginsenoside peaks shown on the HPLC chromatogram would be a very acceptable strategy for discrimination of the cultivation area of ginseng roots.

• Journal of the Korea Society of Computer and Information
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• v.10 no.3 s.35
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• pp.297-306
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• 2005

Compendium of arithmetic, geometry, and proportions and proportionality' that was published in Venice in 1494 has been recognized as the first bookkeeping data in the world. Major characteristics of Pacioli's bookkeeping rules were reviewed in this study as follows: All the necessary particulars for double entry bookkeeping were provided in Pacioli's bookkeeping rules;. List of property was described at the time of start of business; Three major books were used; Details of daily transactions were considered to be important; Strike through was lined at each description in journal books, details of daily transactions, and list of property after entry of ledger; Amount columns were provided and Arabic numerals were used; Annual settlement custom was being initiated; Profit and loss account was prepared at year-end; Trial balance sheet was inevitably described; Books were verified prior to closing accounts; Control account was not established; Financial statements were not prepared and business analysis was made, too; Finished goods inventory was not adjusted; Mark was assigned to books; Inter-office account was prepared; Branch accounts and branch ledgers were prepared; There was entries of trust; Current arrangement was described; The principle 'Cost or market price, whichever is lower basis' was promoted: Petty cash system is explained Checks and bills of exchange are used in bank account. As mentioned, characteristics of Pacioli's bookkeeping rules were reviewed; the signs of necessity for preparation of profit and loss statement and balance sheet is found as well as preparation of trial balance sheet and the rules may be considered as a very excellent one in terms of the bookkeeping on initiating stage of double entry bookkeeping.