• Title/Summary/Keyword: Damage tolerance factor

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Enhanced Tolerance to Oxidative Stress of Transgenic Potato (cv. Superior) Plants Expressing Both SOD and APX in Chloroplasts (SOD와 APX를 동시에 엽록체에 발현시킨 형질전환 감자 (cv. Superior)의 산화스트레스 내성 증가)

  • Tang, Li;Kwon, Suk-Yoon;Kim, Myoung-Duck;Kim, Jin-Seog;Kwak, Sang-Soo;Lee, Haeng-Soon
    • Journal of Plant Biotechnology
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    • v.34 no.4
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    • pp.299-305
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    • 2007
  • Oxidative stress is a major damaging factor for plants exposed to environmental stresses. Previously, we have generated transgenic potato (cv. Superior) plants expressing both CuZnSOD and APX genes in chloroplast under the control of an oxidative stress-inducible SWPA2 promoter (referred to as SSA plants) and selected the transgenic potato plant lines with tolerance against methyl viologen (MV)-mediated oxidative stress. When leaf discs of SSA plants were subjected to $3{\mu}M$ methyl viologen (MV), they showed approximately 40% less damage than non-transgenic (NT) plants. SSA plantlets were treated with $0.3{\mu}M$ MV stress, SSA plants also exhibited reduced damage in root growth. When 350 MV was sprayed onto the whole plants, SSA plants showed a significant reduction in visible damage, which was approximately 75% less damage than leaves of NT plants. These plants will be used for further analysis of tolerance to environmental stresses, such as high temperature and salt stress. These results suggest that transgenic potato (cv. Superior) plants would be a useful plant crop for commercial cultivation under unfavorable growth conditions.

Biotic and spatial factors potentially explain the susceptibility of forests to direct hurricane damage

  • Kim, Daehyun;Millington, Andrew C.;Lafon, Charles W.
    • Journal of Ecology and Environment
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    • v.43 no.4
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    • pp.364-375
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    • 2019
  • Background: Ecologists continue to investigate the factors that potentially affect the pattern and magnitude of tree damage during catastrophic windstorms in forests. However, there still is a paucity of research on which trees are more vulnerable to direct damage by winds rather than being knocked down by the fall of another tree. We evaluated this question in a mixed hardwood-softwood forest within the Big Thicket National Preserve (BTNP) of southeast Texas, USA, which was substantially impacted by Hurricane Rita in September 2005. Results: We showed that multiple factors, including tree height, shade-tolerance, height-to-diameter ratio, and neighborhood density (i.e., pre-Rita stem distribution) significantly explained the susceptibility of trees to direct storm damage. We also found that no single factor had pervasive importance over the others and, instead, that all factors were tightly intertwined in a complex way, such that they often complemented each other, and that they contributed simultaneously to the overall susceptibility to and patterns of windstorm damage in the BTNP. Conclusions: Directly damaged trees greatly influence the forest by causing secondary damage to other trees. We propose that directly and indirectly damaged (or susceptible) trees should be considered separately when assessing or predicting the impact of windstorms on a forest ecosystem; to better predict the pathways of community structure reorganization and guide forest management and conservation practices. Forest managers are recommended to adopt a holistic view that considers and combines various components of the forest ecosystem when establishing strategies for mitigating the impact of catastrophic winds.

Effect of Rhemanniae Radix on the Hyperglycemic Mice Induced with Streptozotocin (생지황(Rhemanniae Radix)이 Streptozotocin으로 유발된 고혈당 생쥐에 미치는 영향)

  • 김정상
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.33 no.7
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    • pp.1133-1138
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    • 2004
  • This study has been carried out to investigate the effect of the administration of Rhemanniae Radix extract (5.0 mL/kg/day, RR group) on the hyperglycemic mice (HM group) induced with streptozotocin (STZ). In blood glucose level, RR group showed a significant decrease compared with HM group. The result of glucose tolerance test was more favorable in RR than HM group. A lot of insulin-positive cells and insulin-like growth factor-II positive materials were observed in RR group. A number of apoptotic particles were observed in the HM group, but several apoptotic nuclei were found in RR group. Pancreatic islets of HM group were destructed by the administration of STZ, but islets were recovered from damage in the RR group. These results suggest that administration of Rhemanniae Radix extract to the hyperglycemic mice prevent from the damage induced by STZ.

An Empirical Approach to Analyze Creep Rupture Behavior of P91 Steel

  • Aslam, Muhammad Junaid;Gur, Cemil Hakan
    • Korean Journal of Materials Research
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    • v.31 no.5
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    • pp.255-263
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    • 2021
  • P91 steel has been a highly researched material because of its applicability for high-temperature applications. Considerable efforts have been made to produce experimental creep data and develop models for creep life prediction. As creep tests are expensive and difficult to conduct, it is vital to develop authenticated empirical methods from experimental results that can be utilized for better understanding of creep behavior and can be incorporated into computational models for reliable prediction of creep life. In this research, a series of creep rupture tests are performed on the P91 specimens within a stress range of 155 MPa to 200 MPa and temperature range of 640 ℃ (913 K) to 675 ℃ (948 K). The microstructure, hardness, and fracture surfaces of the specimens are investigated. To analyze the results of the creep rupture tests at a macro level, a parameter called creep work density is derived. Then, the relationships between various creep parameters such as strain, strain rate, time to rupture, creep damage tolerance factor, and creep work density are investigated, and various empirical equations are obtained.

Fatigue Life Prediction using Fuzzy Reliability theory (퍼지신뢰성이론에 의한 피로수명 예측)

  • 심확섭;이치우;장건의
    • Proceedings of the Korean Society of Precision Engineering Conference
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    • 1995.10a
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    • pp.672-675
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    • 1995
  • Because of a sudden growth of the research of fatigue failure, recent machines or structures have been designed by damage tolerance design in many fields. Consequently, it is the most primary factor to clarity the specific character of fatique failure in the design of machines or structures considering reliability. A statistical analysis is required to analyze the outcome of an experiment or a life estimate by reason of that fatigue failure contains lots of random elements. Reliability analysis which has tukenn the place of the existing analyses in the consideration of the uncertainty of a material, is a very efficient way. Even reliability analysis, however, is not a perfect way to analyses the uncertainties of all the materials. This thesis would refer to a newly conceived data analysis that the coefficient of a system could cause the ambiguity of the relationship of an input and output.

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Prevention of UV-induced Skin Damage by Activation of Tumor Suppressor Genes p53 and $p14^{ARF}$

  • Petersen, R.;John, S.;Lueder, M.;Borchert, S.
    • Proceedings of the SCSK Conference
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    • 2003.09a
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    • pp.338-351
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    • 2003
  • UV radiation is the most dangerous stress factor among permanent environmental impacts on human skin. Consequences of UV exposure are aberrant tissue architecture, alterations in skin cells including functional changes. Nowadays new kinds of outdoor leisure-time activities and changing environmental conditions make the question of sun protection more important than ever. It is necessary to recognize that self-confident consumers do not consider to change their way of life, they demand modern solutions on the basis of new scientific developments. In the past one fundamental principle of cosmetics was the use of physical and organic filter systems against damaging UV-rays. Today new research results demonstrate that natural protecting cell mechanisms can be activated. Suitable biological actives strongly support the protection function not from the surface but from the inside of the cell. A soy seed preparation (SSP) was proven to stimulate natural skin protective functions. The major functions are an increased energy level and the prevention of DNA damage. These functions can I be defined as biological UV protection. The tumor suppressor protein p53 plays a key role in the regulation of DNA repair. p53 must be transferred into the phosphorylated form to work as transcription factor for genes which are regulating the cell cycle or organizing DNA repair. A pretreatment with SSP increases the phosphorylation rate of p53 of chronically UV-irradiated human keratinocytes significantly. According to the same test procedure SSP induces a dramatic increase in the expression of the tumor suppressor protein p14$^{ARF}$ that is supporting the p53 activity by blocking the antagonist of p53, the oncoprotein Mdm2. Mdm2, a ubiquitin E3-ligase, downregulates p53 and at the same time it prevents phosphorylation of p53. The positive influence of the tumor suppressor proteins explains the stimulation of DNA repair and prevention of sunburn cell formation by SSP, which was proven in cell culture experiments. In vivo the increased skin tolerance against UV irradiation by SSP could be confirmed too. We have assumed, that an increased repair potential provides full cell functionality.y.

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Effect of Planting Site and Direction of Fruiting on Fruit Frost Damage in Persimmon (Diospyros kaki 'Fuyu') Fruits from Environment-friendly Orchard (친환경 과원의 단감 '부유'(Diospyros kaki 'Fuyu') 과실의 동상해에 미치는 나무의 식재위치와 착과방향의 영향)

  • Kang, Sung-Ku;Ahn, Kwang-Hwan;Choi, Seong-Tae;Do, Kyung-Ran;Cho, Kwang-Sik
    • Korean Journal of Organic Agriculture
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    • v.22 no.4
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    • pp.789-799
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    • 2014
  • We have analyzed the effect of planting site (elevation) and direction of fruiting on the frost damage (tolerance to transparent skin and blemishes) of persimmon (Diospyros kaki Thunb) 'Fuyu' fruit subjected to the early fall frost (November 2, 2012) in environment-friendly orchards of Changnyeong and Changwon (Gyeongsangnam-do Korea). In Changnyeong, the direction of fruiting showed a significant effect on the fruit weight and the fruit width (p<0.01), and the fruit firmness (p<0.05) while the planting site (elevation) did a highly significant effect on the fruit width and the frost damage (p<0.01), and fruit length (p<0.05). In particular, severe frost damage of fruit (15.2%) and leaves (about 90%) was observed in the plants located in the low elevation area of orchard. The damaged fruit had an transparent skin color and/or blemishes. In Changwon, there was no effect of the planting site and the direction of fruiting on the frost damage, and the fruit characteristics except the fruit width having been related to the planting site (p<0.05). So, the main effects for the fruit frost damage was a terrain factor around the orchards.

Growing pigs developed different types of diabetes induced by streptozotocin depending on their transcription factor 7-like 2 gene polymorphisms

  • Tu, Ching-Fu;Hsu, Chi-Yun;Lee, Meng-Hwan;Jiang, Bo-Hui;Guo, Shyh-Forng;Lin, Chai-Ching;Yang, Tien-Shuh
    • Laboraroty Animal Research
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    • v.34 no.4
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    • pp.185-194
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    • 2018
  • The different polymorphisms of the transcription factor 7-like 2 (TCF7L2) gene promote variances in diabetes susceptibility in humans. We investigated whether these genotypes also promote differences in diabetic susceptibility in commercial pigs. Growing pigs (Landrace, both sex, 50-60 kg) with the C/C (n=4) and T/T (n=5) TCF7L2 genotypes were identified and intravenously injected with streptozotocin (STZ, 40 mg/kg) twice in weekly intervals, then a high-energy diet was offered. Oral glucose tolerance tests, blood analyses and the homeostasis model assessment-insulin resistance (HOMA-IR) index calculations were performed. The animals were sacrificed at the end of 12 weeks of treatment to reveal the pancreas histomorphometry. The results showed that all of the treated pigs grew normally despite exhibiting hyperglycemia at two weeks after the induction. The glycemic level of the fasting or postprandial pigs gradually returned to normal. The fasting insulin concentration was significantly decreased for the T/T carriers but not for the C/C carriers, and the resulting HOMA-IR index was significantly increased for the C/C genotype, indicating that the models of insulin dependence and resistance were respectively developed by T/T and C/C carriers. The histopathological results illustrated a significant reduction in the pancreas mass and insulin active sites, which suggested increased damage. The results obtained here could not be compared with previous studies because the TCF7L2 background has not been reported. Growing pigs may be an excellent model for diabetic in children if the animals are genetically pre-selected.

A study on seismic behaviour of masonry mosques after restoration

  • Altunisik, Ahmet C.;Bayraktar, Alemdar;Genc, Ali F.
    • Earthquakes and Structures
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    • v.10 no.6
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    • pp.1331-1346
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    • 2016
  • Historical masonry structures have an important value for cultures and it is essential for every society to strengthen them and confidently transfer to the future. For this reason, determination of the seismic earthquake response, which is the most affecting factor to cause the damage at these structures, gain more importance. In this paper, the seismic earthquake behaviour of Kaya Çelebi Mosque, which is located in Turkey and the restoration process has still continued after 2011 Van earthquake, is determined. Firstly the dynamic modal analysis and subsequently the seismic spectral analysis are performed using the finite element model of the mosque constructed with restoration drawings in SAP2000 program. Maximum displacements, tensile, compressive and shear stresses are obtained and presented with contours diagrams. Turkish Earthquake Code and its general technical specifications are considered to evaluate the structural responses. After the analyses, it is seen that the displacements and compressive/shear stresses within the code limits. However, tension stresses exceeded the maximum values at some local regions. For this mosque, this is in tolerance limits considering the whole structure. But, it can be said that the tension stresses is very important for this type of the structures, especially between the stone and mortar. So, some additional strengthening solutions considering the originality of historical structures may be applicable on maximum tensile regions.

Creep and creep crack growth behaviors for base, weld, and heat affected zone in a grade 91 weldment

  • Kim, Woo-Gon;Sah, Injin;Kim, Seon-Jin;Lee, Hyeong-Yeon;Kim, Eung-Seon
    • Nuclear Engineering and Technology
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    • v.53 no.2
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    • pp.572-582
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    • 2021
  • This study investigated the creep and creep crack growth (CCG) behavior of the base metal (BM), weld metal (WM), and heat affected zone (HAZ) in a Gr. 91 weldment, which was made by a shield metal arc weld process. A series of tensile, creep, and CCG tests were performed for the BM, WM, and HAZ at 550 ℃. Creep behavior of the BM, WM, and HAZ was analyzed in terms of various creep laws; Norton's power-law, Monkman-Grant relation and damage tolerance factor (λ), and their constants were determined. In addition, each CCGR law for the BM, WM, and HAZ was proposed and compared in terms of a C*-fracture parameter. The WM and HAZ revealed faster creep rate, lower rupture ductility, and faster CCGRs than the BM, but they showed a similar behavior in the creep and CCG. The CCGRs obtained in the present study exhibited a marginal difference when compared with those of RCC-MRx of currently elevated design code in France. A creep crack path in the HAZ plane progressed towards a weak fine-grained HAZ adjacent to the BM.