• Korean Journal of Medicinal Crop Science
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• v.17 no.4
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• pp.273-279
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• 2009

GATA-binding protein-3 (GATA-3) and T-box expressed in T-cells (T-bet) are now considered as master transcription factors involving Th cell differentiation, but the roles of these factors are still uncertain in vivo. This study was conducted to investigate the expression of these transcription factors in the liver damage induced by carbon tetrachloride ($CCl_4$) in rats. In this study, liver damage were induced with Scutellaria baicalensis Georgi water extracts (SBW) and followed for 4 weeks. The expression of GATA-3 and T-bet protein in liver damage induced by $CCl_4$ and the serum levels of immunoglobulin A (IgA), IgE were studied after 4 weeks of treatment. We found that effect of SBW on IFN-$\gamma$, STAT1, pSTAT1 and T-bet was decreased in vivo. Several genes were demonstrated to be IL-4 inducible prior to the discovery of STAT6. $CCl_4$+SBW group was significantly lower than $CCl_4$ group in IL-4, STAT6, pSTAT6 and GATA-3. Our data indicate that cytokine protein production were increased in $CCl_4$ group and $CCl_4$+SBW group. From these results, water extracts obtained from Scutellaria baicalensis Georgi may have an immunoregulatory effect in the liver induced by $CCl_4$ of rats.

• The Korea Journal of Herbology
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• v.29 no.5
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• pp.91-95
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• 2014

Objectives : This study was carried out to evaluate whether the water extract from cause the cellular damage in HepG2 cell line. It was reported that Dictamnus dasycarpus Turcz(DDT) intake induce poisoning symptoms in human population. These symptoms was closely related to liver toxicity, however, mechanisms for liver toxicity caused by DDT have not been elucidated exactly. Here, hepatotoxicity caused by DDT was evaluated using HepG2 cell line. Methods : Water extract of DDT was treated into HepG2 cell with various doses such as 0, 0.1, 0.5, 1.0 and $5.0mg/m{\ell}$. In order to cell viability, both MTT and LDH assay were carried out. Also, apoptosis array kit was used to identify whether cell death caused by DDT is due to apoptosis or not. In addition, reactive oxygen species (ROS) was measured after treatment of water extract. Results : We found out significant changes in the apoptosis related factors of hepatocyte. The cell viability of HepG2 treated with DDT water extract was decreased in dose-dependent. Also most of the apoptosis related factors were significantly increased. We found out that Caspase 3, Cytochrome C and ROS had increased in dose-dependent. In addition, other apoptosis related factors Bcl 2 and Bax, which were also constant changes. However, there was no significance. Conclusions : These results suggest that water soluble extract of DDT is expected to have oral toxicity, including hepatocellular damage Therefore, it is suggested that DDT could cause various side effects and toxicity of clinical conditions.

• Structural Engineering and Mechanics
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• v.52 no.6
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• pp.1157-1176
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• 2014

The objective of this research is to develop a practical design and assessment approach of steel frames with steel slit walls (SSWs) that focuses on the damage-control behavior to enhance the structural resilience. The yielding sequence of SSWs and frame components is found to be a critical issue for the damage-control behavior and the design of systems. The design concept is validated by the full-scale experiments presented in this paper. Based on a modified energy-balance model, a procedure for designing and assessing the system motivated by the framework regarding the equilibrium of the energy demand and the energy capacity is proposed. The damage-control spectra constructed by strength reduction factors calculated from single-degree-of-freedom systems considering the post stiffness are addressed. A quantitative damage-control index to evaluate the system is also derived. The applicability of the proposed approach is validated by the evaluation of example structures with nonlinear dynamic analyses. The observations regarding the structural response and the prediction during selected ground motions demonstrate that the proposed approach can be applied to damage-control design and assessment of systems with satisfactory accuracy.

• Environmental Mutagens and Carcinogens
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• v.22 no.2
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• pp.83-89
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• 2002

The single cell gel electrophoresis (comet) assay is one of the useful tools for the study of genetic damage in humans exposed to environmental mutagens and carcinogens. This study was undertaken to evaluate the status of DNA damage in peripheral lymphocytes depending on their sex, age, smoking habits, and other factors in normal healthy Korean population. The 99 volunteers included in the study and out of these, 36 volunteers were smoker and 63 volunteers were non-smoker aged between 20-59 years. All individual answered a questionnaire that assessed their general information including smoking habits and the extent of the environmental tobacco smoke (ETS) exposure, and blood samples were obtained. There was a statistically significant difference in the extent of DNA damage between smoker and non-smoker (p<0.001). A significant difference was also observed between male and female (p<0.001) and amongst the different group of age (p<0.005), however, correlation analysis showed that only smoking habit was a significant factor for DNA damage. No significant effect of smoking duration, number of cigarettes smoking a day, SPY (smoke pack years) in smokers and environmental tobacco smoke exposure in non-smokers on the status of DNA damage was observed.

• BMB Reports
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• v.47 no.5
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• pp.249-255
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• 2014

Polo-like kinase-1 (Plk1) belongs to a family of serine-threonine kinases and plays a critical role in mitotic progression. Plk1 involves in the initiation of mitosis, centrosome maturation, bipolar spindle formation, and cytokinesis, well-reported as traditional functions of Plk1. In this review, we discuss the role of Plk1 during DNA damage response beyond the functions in mitotsis. When DNA is damaged in cells under various stress conditions, the checkpoint mechanism is activated to allow cells to have enough time for repair. When damage is repaired, cells progress continuously their division, which is called checkpoint recovery. If damage is too severe to repair, cells undergo apoptotic pathway. If damage is not completely repaired, cells undergo a process called checkpoint adaptation, and resume cell division cycle with damaged DNA. Plk1 targets and regulates many key factors in the process of damage response, and we deal with these subjects in this review.

• Computers and Concrete
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• v.21 no.1
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• pp.55-66
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• 2018

A large main shock may consist of numerous aftershocks with a short period. The aftershocks induced by a large main shock can cause the collapse of a structure that has been already damaged by the preceding main shock. These aftershocks are important factors in structural damages. Furthermore, despite what is often assumed in seismic design codes, earthquakes do not usually occur as a single event, but as a series of strong aftershocks and even fore shocks. For this reason, this study investigates the effect and potential of consecutive earthquakes on the response and behavior of concrete structures. At first, six moment resisting concrete frames with 3, 5, 7, 10, 12 and 15 stories are designed and analyzed under two different records with seismic sequences from real and artificial cases. The damage states of the model frames were then measured by the Park and Ang's damage index. From the results of this investigation, it is observed that the sequences of ground motions can almost double the accumulated damage and increased response of structures. Therefore, it is certainly insufficient to ignore this effect in the design procedure of structures. Also, the use of artificial seismic sequences as design earthquake can lead to non-conservative prediction of behavior and damage of structures under real seismic sequences.

• Journal of Korean Society of Rural Planning
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• v.24 no.1
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• pp.61-66
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• 2018

The purpose of this study is to identify the factors related to the heavy rain damage and to identify effect of repair and improvement for irrigation facilities on heavy rain damages. The results of the analysis are as follows. First, the imbalance of precipitation became worse over time from using the coefficient of variation. Second, the analysis using Spearman correlation coefficient shows positive relationship between heavy rain damage amount and precipitation amount, and negative correlation between heavy rain damage amount and repair and improvement for irrigation facilities cost. Third, the analysis of the panel regression model shows that the negative impact of the repair and improvement for irrigation facilities cost on the heavy rain damage, which means that the increase of the repair and improvement for irrigation facilities cost can reduce the heavy rain damage.

• Computational Structural Engineering : An International Journal
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• v.3 no.1
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• pp.9-17
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• 2003

This paper aims at investigating the damage process of steel members leading to the failure under strong repeated loading, proposing the damage index using various factors related to the damage, and developing the analysis method for evaluating the damage state. Cantilever-type steel members were analyzed under uniaxial load and combined with a constant axial load, considering a horizontal displacement history. In analyzing the models, loading patterns and steel types (SS400, SM570, Posten80) were considered as main parameters. From the analysis results, the effects of parameter on the failures mode, the deformation capacity, the damage process are also discussed. Each failure process was compared as steel types. Consequently, the failure of steel members under strong repeated loading was determined by loading. Especially it was seen that the state of the failure is closely related to the local strain.

• Journal of the Korean Geotechnical Society
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• v.28 no.7
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• pp.55-65
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• 2012

Total reduction factor that is used when calculating allowable tensile strength of geogrids is made by multiplying the installation damage reduction factor ($RF_{ID}$), chemical degradation reduction factor ($RF_D$), and creep reduction factor ($RF_{CR}$) etc. In case of a model estimating allowable tensile strength considering reduction factor over the short-term tensile strength of geogrids, it has a limit of not considering interaction force between reduction factors. Junction strength comes to be reduced by installation damages or chemical degradation in the same way as tensile strength. Single junction test method cannot properly test damaged samples and shows large deviations as it does not consider scale effect. Besides, regarding calculating shear strength, no reasonable study on reduction factors was conducted yet. Therefore, in this study, reduction factors that may affect the long-term performance of geogrids were revaluated considering various conditions and accurate long-term allowable tensile strength was calculated considering interrelation between reduction factors. Creep results after installation damage and chemical resistance test showed lower value than calculated value according to GRI GG-4. After the installation damage test and the chemical resistance test, the reduction factor of junction strength was less than that of tensile strength. Shear strength before and after installation damage showed no change or increase.

• Journal of Environmental Science International
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• v.19 no.3
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• pp.353-363
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• 2010

The most efficient measures to reduce damage from natural disasters include activities which prevent disasters in advance, decrease possibility of disasters and minimize the scale of damage. Therefore, developing of the risk assessment model is very important to reduce the natural disaster damage. This study estimated a typhoon damage which is the biggest damage scale among increased natural disasters in Korea along with climate change. The results of 3-second gust at the height of 10m level from the typhoon 'Maemi' which did considerable damage to Korean in 2003, using the wind data at the height of 700 hPa. September 12th 09 LST~13th 12 LST period by the time a typhoon Maemi approached to the Korean peninsula. This study estimate damage amount using 'Fragility curve' which is the damage probability curve about a certain wind speed of the each building component factors based on wind load estimation results by using 3-second gust. But the fragility curve is not to Korea. Therefore, we use the fragility curves to FPHLM(FDFS, 2005). The result of houses damage amount is about 11 trillion 5 million won. This values are limit the 1-story detached dwelling, $62.51\sim95.56\;m^2$ of total area. Therefore, this process is possible application to other type houses.