The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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The effects of water extract from Dictamnus dasycarpus Turcz on Hepatocellular Damage in vitro

백선 추출물의 간세포 손상에 대한 연구

  • Ha, Hun-Yong (Department of National Medicine Resources, Semyung University)
  • 하헌용 (세명대학교 자연약재과학과)
  • Received : 2014.08.04
  • Accepted : 2014.09.11
  • Published : 2014.09.30
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Abstract

Objectives : This study was carried out to evaluate whether the water extract from cause the cellular damage in HepG2 cell line. It was reported that Dictamnus dasycarpus Turcz(DDT) intake induce poisoning symptoms in human population. These symptoms was closely related to liver toxicity, however, mechanisms for liver toxicity caused by DDT have not been elucidated exactly. Here, hepatotoxicity caused by DDT was evaluated using HepG2 cell line. Methods : Water extract of DDT was treated into HepG2 cell with various doses such as 0, 0.1, 0.5, 1.0 and $5.0mg/m{\ell}$. In order to cell viability, both MTT and LDH assay were carried out. Also, apoptosis array kit was used to identify whether cell death caused by DDT is due to apoptosis or not. In addition, reactive oxygen species (ROS) was measured after treatment of water extract. Results : We found out significant changes in the apoptosis related factors of hepatocyte. The cell viability of HepG2 treated with DDT water extract was decreased in dose-dependent. Also most of the apoptosis related factors were significantly increased. We found out that Caspase 3, Cytochrome C and ROS had increased in dose-dependent. In addition, other apoptosis related factors Bcl 2 and Bax, which were also constant changes. However, there was no significance. Conclusions : These results suggest that water soluble extract of DDT is expected to have oral toxicity, including hepatocellular damage Therefore, it is suggested that DDT could cause various side effects and toxicity of clinical conditions.

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References

  1. Lee MH, Jo DJ, Yoon SR, Lee GD. Physicochemical properties of functional herb mixtures. J Korean Soc Food Sci Nutr. 2007 ; 36(12) : 1571-7. https://doi.org/10.3746/jkfn.2007.36.12.1571
  2. Kim HJ, Jun BS, Kim SK, Cha JY, Cho YS. Polyphenolic compound content and antioxidative activities by extracts from seed, sprout and flower of safflower(Carthamus Tinctorius L.). J Korean Soc Food Sci Nutr. 2000 ; 29 : 1127-32.
  3. Mun SP, Koo DS, Park SB, Kwon SD. Characteristics of bamboo smoke distillates made by three kinds carbonization kiln. Korean Soc Food Sci Technol Annual Meeting. 2000 ; 1 : 252-57.
  4. Lee JM, Son ES, Oh SS, Han DS. Contents of total flavonoid and biological activities of edible plants. Korean J Dietary Culture. 2001 ; 16 : 504-14.
  5. Ahn DK, Kim CS. A study on the side effect of crude drugs. Kor J Pharmacogn. 1983 ; 14(3) : 102-6.
  6. Hwang SY, Kwon W, Chai HY, Cho YM, Lee NJ, Ryu JM, Sin JS, Kim TM, Cho JH, Kim EJ, Park JH, Kang JK, Kim YB. Four-week repeated-dose toxicity study on Mori Radicis Cortex. Korean J Lab Animal Sci. 2004 ; 20(3) : 283-90.
  7. Jung JH, Kim SH, Ko KH, Jung KH, Hwang SW, Goh PG, Park NH, Nam GW, Kim JI, Moon HS, Lee ES. Clinical features of 28 acutely toxic hepatitis patients who ingested Dictamnus Dasycarpus: A single center clinical experience. Korean J Med. 2010 ; 78(4) : 457-65.
  8. An SY, Cheng JY, Kim SS, Lee DM, Seok JY, Kim YB, Cho SW. One case of fulminant hepatic failure related to Dictamnus Dasycarpus. Korean J Med. 2010 ; 78(4) : 490-4.
  9. Lee JH, Lee HY, Koh KC, Lee JK, Rhee PL, Kim JJ, Paik SW, Rhee JC, Oh YR, Chi YJ. Drug induced liver disease caused by ingestion of Dyctamnus Dasycarpus. Korean J Gastroenterol. 1998 ; 31(2) : 251-7.
  10. Kim SH, Cho WY, Kim GH, Jang JY, Shim CS, Kim BS, Jin SY. Case reports: Gastroenterology; A case of acute hepatitis caused by ingesting Dictamnus Dasycarpus. Korean J Med. 2009 ; 76(4) : 476-80.
  11. Choi HG, Lee DS, Li B, Jun KY, Jeong GS, Kim YC. Neuroprotective effect of the water-insoluble fraction of root barks of Dictamnus Dasycarpus 70% ethanolic extract on glutamate-induced oxidative damage in mouse hippocampal HT22 cells. Kor J Pharmacogn. 2011 ; 42(2) : 175-81.
  12. Park JS, Shin TY, Kim DG, Lee JH. The effects of Dictamni Radicis Cortex on the iNOS expression and proinflammatory cytokines production. Kor J Pharmacogn. 2011 ; 42(4) : 348-53.
  13. Qin M, Guo HB, Xu Y. Cortex Dictamni inhibits formation of early atherosclerotic lesions in apoE-deficient mice. ACTA Laboratorium Animalis Scientica Sinica. 2010 ; 18(3) : 191-5.
  14. Hu XM, Zhang WK, Zhang HJ, Dong HM, Xie KW, Wu BX, Zhonghwabencao jingxuanben. 2nd ed. Shanghai : kejichubanshe. 1998 : 1023-6.
  15. Ha HY. The another name of herbal medicine. 1st ed. Seoul : passanpass. 2007 : 460.
  16. Kim KS. Present status and perspectives of medicinal plant resources in korea. Res Nati Resources. 1999 ; 2 : 25-41.
  17. Kang BH. Ecological flora of surrounding resource plants for biological diversity and green survival. 1st ed. Paju : Korean Studies Information. 2013 : 316.
  18. Kweon KT. A reasearch on management system of herbal medicine in common use for food and medicine. Kor J Herbology. 2012 ; 27(2) : 25-9. https://doi.org/10.6116/kjh.2012.27.2.25
  19. Kim SK, Jang HC, Kim JH, Kim C, Yea SJ, Song MY. A searching service of toxic and contraindicating information of medicinal materials in traditional korean medicine. Korea J Orient Med. 2011 ; 17(3) : 69-76.
  20. Jeong HW, Jeon BH. The cytotoxic effect of Dictamni Cortex Radicis and Manitis Squama on human cancer cell-lines. Korean J Orient Physiol Pathol. 1997 ; 11(1) : 58-64.
  21. Han MH, Lee MH, Hong SH, Choi YH, Moon JS, Song MK, Kim MJ, Shin SJ, Hwang HJ. Comparison of anti-inflammatory activities among ethanol extracts of sophora flavescens, Glycyrrhiza Uralensis and Dictamnus Dasycarpus, and their mixtures in RAW 246.7 murine macrophages. J Life Sci. 2014 ; 24(3) : 329-35. https://doi.org/10.5352/JLS.2014.24.3.329