• 대한본초학회지
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• 제35권4호
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• pp.9-16
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• 2020

Objective : To identify the effects of the water extract of Liriope platyphylla tuber (Liriopis tuber, LT) on the activation of astocytes, we investigated the regulatory effects of LT extract on H₂O₂-induced oxidative damage in C6 rat astrocytes. Methods : LT extract was extracted with boiling water. C6 cell line were treated with LT extract at 1, 2, and 3 mg/㎖ or without for 30 min and then stimulated with H₂O₂ at 5 ㎛ for 24 hr. The cell viability was measured by MTT assay. The expression of glial fibrillary acidic protein (GFAP), signal transducer and activator of transcription 3 (STAT3), phospho-STAT3 (pSTAT3), cyclooxygenase (COX-2), Nuclear factor-κB (NF-κB), superoxide dismutase 2 (SOD2), heme oxygenase-1 (HO-1), catalase, Akt, phospho-Akt (p-Akt) phosphoinositide 3-kinases (PI3K), and protein kinase C alpha (PKCα) proteins were determined by Western blot, respectively. GFAP expression was also observed with immunocytochemistry under a fluorescence microscope. Results : LT extract induced cell proliferation in H₂O₂-stimulated C6 cells. LT extract significantly inhibited the expression of GFAP, NF-κB and COX-2 and increased the expression of HO-1 and the phosphorylation of STAT3 in H₂O₂-stimulated C6 cells. LT extract also significantly increased the phosphorylation of Akt and decreased the expression of PKCα in a dose-dependent manner in H₂O₂-stimulated C6 cells. Conclusions : LT extract can regulate H₂O₂-induced activation of astrocytes through inhibiting the expression of NF-κB, COX-2 and regulating Akt / HO-1, STAT3 or PKCα signaling pathway.

• 농업과학연구
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• 제7권1호
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• pp.1-5
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• 1980

수도(水稻) 신품종(新品種)들의 내냉성(耐冷性) 판별(判別)과 냉해대책(冷害對策)을 위하여 생식생장기(生殖生長期)에 있어서 저온(低溫)의 정도(程度)와 기간(期間)이 임실(稔實)과 등숙(登熟)에 미치는 영향(影響)을 검토(檢討)하여 다음과 같은 결과(結果)를 얻었다. 1. 수도(水稻) 감수분열기(減數分裂期)에 있어서 저온(低溫)의 정도(程度)가 크고 기간(期間)이 길어 질수록 출수(出穗)가 지연(遲延)되고 불임(不稔)이 증가(增加)하였으며 저온(低溫)에 의(依)한 감수(減收)는 주(主)로 불임증가(不稔增加)에 기인(基因)하였으나 $15^{\circ}C$의 저온(低溫)에서는 출수(出穗) 지연(遲延)도 관여(關與)하는 것으로 보였다. 2. $15^{\circ}C$ 6일간(日間) 처리(處理)에서는 불임(不稔)과 발육정지립(發育停止粒)이 다 같이 증가(增加)하고 완전(完全) 발숙립(發熟粒)은 거의 없었다. 3. 신품종(新品種)들은 기존(旣存) 품종(品種)보다 생식생장기(生殖生長期) 냉해(冷害)가 현저(顯著)하였고 내냉성(耐冷性) 검정(檢定)의 parameter로서는 감수분열기(減數分裂期)의 $15^{\circ}C$ 4일간(日間) 처리(處理)가 적당(適當)한 것으로 보였다.

• Biomolecules & Therapeutics
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• 제29권2호
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• pp.227-233
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• 2021

Benzo[a]pyrene (B[a]P) is a polycyclic aromatic hydrocarbon and ubiquitous environmental toxin with known harmful effects to human health. Abnormal phenotypes of keratinocytes are closely associated with their exposure to B[a]P. Resorcinol is a component of argan oil with reported anticancer activities, but its mechanism of action and potential effect on B[a]P damage to the skin is unknown. In this study, we investigated the effects of resorcinol on B[a]P-induced abnormal keratinocyte biology and its mechanisms of action in human epidermal keratinocyte cell line HaCaT. Resorcinol suppressed aryl hydrocarbon receptor (AhR) activity as evidenced by the inhibition of B[a]P-induced xenobiotic response element (XRE)-reporter activation and cytochrome P450 1A1 (CYP1A1) expression. In addition, resorcinol attenuated B[a]P-induced nuclear translocation of AhR, and production of ROS and pro-inflammatory cytokines. We also found that resorcinol increased nuclear factor (erythroid-derived 2)-like 2 (Nrf2) activity. Antioxidant response element (ARE)-reporter activity and expression of ARE-dependent genes NAD(P)H dehydrogenase [quinone] 1 (NQO1), heme oxygenase-1 (HO-1) were increased by resorcinol. Consistently, resorcinol treatment induced nuclear localization of Nrf2 as seen by Western analysis. Knockdown of Nrf2 attenuated the resorcinol effects on ARE signaling, but knockdown of AhR did not affect resorcinol activation of Nrf2. This suggests that activation of antioxidant activity by resorcinol is not mediated by AhR. These results indicate that resorcinol is protective against effects of B[a]P exposure. The mechanism of action of resorcinol is inhibition of AhR and activation of Nrf2-mediated antioxidant signaling. Our findings suggest that resorcinol may have potential as a protective agent against B[a]P-containing pollutants.

• Journal of Nutrition and Health
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• 제54권3호
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• pp.321-333
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• 2021

본 연구에서는 진피-황금 혼합물 (CS)이 급성 역류성 식도염에 미치는 식도 점막 보호 효과를 평가하기 위하여 CS를 경구투여한 후 수술을 통해 역류성 식도염을 유발하였으며, 실험 종료 후 혈액 채취 및 식도 조직을 적출하였다. 동물에게서 적출한 식도 점막의 손상 정도를 육안으로 확인한 결과 CS투여군에서 식도 점막의 손상이 유의하게 감소하였으며, H&E staining을 통해 관찰한 결과 마찬가지로 CS투여군에서 식도 상피의 탈락 및 염증세포의 침윤이 현저하게 감소한 것을 확인하였다. 혈액을 이용하여 역류성 식도염의 원인으로 유효하다고 알려진 ROS의 수치를 확인한 결과, CS투여군에서 ROS 수치가 유의적으로 감소하였으며, western blotting을 통해 NADPH oxidase인 NOX4, p47^phox, p22^phox의 발현을 확인한 결과, 마찬가지로 CS 투여군에서 유의하게 감소하였고, 특히 CS200투여군에서 Normal군과 비슷한 수치를 나타냈다. 또한, CS투여는 염증성 단백질인 MAPK와 NF-κB 경로를 유의적으로 억제하였을 뿐 아니라 tight junction 단백질인 claudin-1과 claudin-4의 발현을 유의하게 조절한 것을 확인하였다. 이상의 결과를 종합해보면 진피-황금 추출물은 산화적 스트레스를 억제함으로써 염증성 단백질의 발현을 조절할 뿐 아니라 tight junction 단백질의 발현을 조절하여 식도 점막을 보호하는 것으로 판단되나 역류성 식도염은 음식물의 섭취와 밀접한 관련이 있는 만큼 추후 동물의 식이 섭취량을 조사하는 등 세부적인 추가 연구가 필요할 것으로 보인다.

• 한국인터넷방송통신학회논문지
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• 제23권2호
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• pp.157-162
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• 2023

태양광발전 설비의 고장 중 서지에 의한 고장이 전체 고장률의 20% 차지하고 있으며 발전 중 수십에서 수백[A]의 에너지 방출과 인버터, 접속반 등의 전기적 손상은 전기안전사고로 이어지고 있다. 특히 낙뢰의 경우 전기회로에 이상 전압이 유기되어 절연을 파괴할 뿐만 아니라 이때 흐르는 전류는 화재의 원인이 되고 부품의 열화를 촉진하는 요인으로 작용한다. 이러한 작용으로 도심 밖에서 주택, 아파트, 관공서 등의 도심 내부로 확산하고 있는 태양광 발전장치의 전기 안전 문제가 대두되고 있다. 낙뢰는 필드 기반 및 전도성 전기 간섭을 유발하기에 이 효과는 케이블 길이 또는 도체 루프 증가와 관련하여 증가한다. 또한 서지는 태양광 모듈, 인버터 및 모니터링장치뿐만 아니라 건물 설비의 장치도 손상하기에 최종적으로는 태양광발전시스템의 화재로 인한 운영 중단과 이에 따른 재정손실을 유발하게 시킬 수 있다. 따라서 본 논문에서는 태양광발전시스템의 낙뢰발생으로 인한 화재 및 전기안전사고 증가로 인하여 재산피해 및 인명피해를 줄일 수 있는 목적으로 태양광발전장치의 낙뢰보호 시스템을 연구하고자 한다.

• Molecules and Cells
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• 제26권5호
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• pp.427-435
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• 2008

In this review, we discuss the genes and the related signal pathways that regulate aging and longevity by reviewing recent findings of genetic longevity models in rodents in reference to findings with lower organisms. We also paid special attention to the genes and signals mediating the effects of calorie restriction (CR), a powerful intervention that slows the aging process and extends the lifespan in a range of organisms. An evolutionary view emphasizes the roles of nutrient-sensing and neuroendocrine adaptation to food shortage as the mechanisms underlying the effects of CR. Genetic and non-genetic interventions without CR suggest a role for single or combined hormonal signals that partly mediate the effect of CR. Longevity genes fall into two categories, genes relevant to nutrient-sensing systems and those associated with mitochondrial function or redox regulation. In mammals, disrupted or reduced growth hormone (GH)-insulin-like growth factor (IGF)-1 signaling robustly favors longevity. CR also suppresses the GH-IGF-1 axis, indicating the importance of this signal pathway. Surprisingly, there are very few longevity models to evaluate the enhanced anti-oxidative mechanism, while there is substantial evidence supporting the oxidative stress and damage theory of aging. Either increased or reduced mitochondrial function may extend the lifespan. The role of redox regulation and mitochondrial function in CR remains to be elucidated.

• 산업경영시스템학회지
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• 제39권4호
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• pp.117-124
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• 2016

Stress is resistance to stimuli to destroy a balance of mind and body in order to maintain an original steady state : A type of response and avoidance. Mild stress, which happens in tension, has a positive effect to improve concentration, but most of stress is likely to be a main factor to damage health. This is because it causes physical ailments and psychological diseases (e.g., depression, anger, frustration, loneliness, and anxiety). The purpose of this study is to analyze the difference between two groups, daytime and night university students, on ego-state, stressors, and stress coping types to improve their academic achievement by reducing their stress. There were 266 university students, who participated in a survey from November 30, 2015 until September 30, 2016. The study employed three measuring instruments : Ego-gram, stressor scale, and stress coping type scale. All the collected data were analyzed through regression analysis and t-test. The results of the regression analysis showed no significant differences in ego-state, stressors, and stress coping types between two groups. However, the results of t-test showed significant difference between the two groups : In terms of ego-state, a CP (Critical Parent) average of the night university students was significantly higher than the daytime university students'. Also, there is a significant difference in employment, future issues, and economic issues : the daytime university students considered employment and future issues more serious, while the night university students had more mental burdens of economic issues. When it comes to coping stress types, there was no significant difference between the two groups.

• 한국지리정보학회지
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• 제14권3호
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• pp.68-77
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• 2011

인구와 시설이 밀집되어 있는 도시지역은 대규모 재해가 발생하는 경우 엄청난 대규모의 피해가 발생 할 수 있으므로 재난에 대한 사후처리뿐 아니라 위험지역을 사전에 파악하고 위험을 저감하기 위한 대책 수립이 필요하다. 현재까지 지역안전도에 대한 많은 연구가 진행되어 왔으나, 나라별, 도시별 특성이 다를 수 있기 때문에 도시지역 특성을 반영한 표준화된 평가기법 및 지표의 개발이 필요하다. 본 연구에서 제시한 지역안전도 평가 모형은 위험 항목별 요인들을 조합한 위험도에 저감성을 고려한 것으로 홍수 위험에 미치는 3가지 요인과 저감성에 영향을 미치는 2가지 요인들을 선정하고, 이들을 대표할 수 있는 12개 인자를 선정한 후 GIS를 이용하여 인자별 평가결과의 가중 합을 통한 중첩으로 지역안전도평가 모형을 제안하였다. 제안된 지역안전도 평가모형을 이용하여 서울시에 대하여 모형의 시범적용을 수행하였다.

• International Journal of Concrete Structures and Materials
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• 제9권2호
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• pp.159-172
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• 2015

Estimation of the residual strength of corroded reinforced concrete beams has been studied from experimental and theoretical perspectives. The former is arduous as it involves casting beams of various sizes, which are then subjected to various degrees of corrosion damage. The latter are static; hence cannot be generalized as new coefficients need to be re-generated for new cases. This calls for dynamic models that are adaptive to new cases and offer efficient generalization capability. Computational intelligence techniques have been applied in Construction Engineering modeling problems. However, these techniques have not been adequately applied to the problem addressed in this paper. This study extends the empirical model proposed by Azad et al. (Mag Concr Res 62(6):405-414, 2010), which considered all the adverse effects of corrosion on steel. We proposed four artificial neural networks (ANN) models to predict the residual flexural strength of corroded RC beams using the same data from Azad et al. (2010). We employed two modes of prediction: through the correction factor ($C_f$) and through the residual strength ($M_{res}$). For each mode, we studied the effect of fixed and random data stratification on the performance of the models. The results of the ANN models were found to be in good agreement with experimental values. When compared with the results of Azad et al. (2010), the ANN model with randomized data stratification gave a $C_f$-based prediction with up to 49 % improvement in correlation coefficient and 92 % error reduction. This confirms the reliability of ANN over the empirical models.

• Biomolecules & Therapeutics
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• 제26권6호
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• pp.576-583
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• 2018

Human rhinoviruses (HRV) are one of the major causes of common cold in humans and are also associated with acute asthma and bronchial illness. Heat-shock protein 90 (Hsp90), a molecular chaperone, is an important host factor for the replication of single-strand RNA viruses. In the current study, we examined the effect of the Hsp90 inhibitor pochonin D, in vitro and in vivo, using a murine model of human rhinovirus type 1B (HRV1B) infection. Our data suggested that Hsp90 inhibition significantly reduced the inflammatory cytokine production and lung damage caused by HRV1B infection. The viral titer was significantly lowered in HRV1B-infected lungs and in Hela cells upon treatment with pochonin D. Infiltration of innate immune cells including granulocytes and monocytes was also reduced in the bronchoalveolar lavage (BAL) by pochonin D treatment after HRV1B infection. Histological analysis of the lung and respiratory tract showed that pochonin D protected the mice from HRV1B infection. Collectively, our results suggest that the Hsp90 inhibitor, pochonin D, could be an attractive antiviral therapeutic for treating HRV infection.