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http://dx.doi.org/10.6116/kjh.2020.35.4.9.

A Study on the Effect of Liriopis tuber water extract on Hydrogen Peroxide-stimulated C6 Astrocyte Cells  

Park, Ki Ho (Department of Herbology, college of Korean Medicine, Dongguk University)
Kang, Seok Yong (Department of Herbology, college of Korean Medicine, Dongguk University)
Jung, Hyo Won (Department of Herbology, college of Korean Medicine, Dongguk University)
Park, Yong-Ki (Department of Herbology, college of Korean Medicine, Dongguk University)
Publication Information
The Korea Journal of Herbology / v.35, no.4, 2020 , pp. 9-16 More about this Journal
Abstract
Objective : To identify the effects of the water extract of Liriope platyphylla tuber (Liriopis tuber, LT) on the activation of astocytes, we investigated the regulatory effects of LT extract on H2O2-induced oxidative damage in C6 rat astrocytes. Methods : LT extract was extracted with boiling water. C6 cell line were treated with LT extract at 1, 2, and 3 mg/㎖ or without for 30 min and then stimulated with H2O2 at 5 ㎛ for 24 hr. The cell viability was measured by MTT assay. The expression of glial fibrillary acidic protein (GFAP), signal transducer and activator of transcription 3 (STAT3), phospho-STAT3 (pSTAT3), cyclooxygenase (COX-2), Nuclear factor-κB (NF-κB), superoxide dismutase 2 (SOD2), heme oxygenase-1 (HO-1), catalase, Akt, phospho-Akt (p-Akt) phosphoinositide 3-kinases (PI3K), and protein kinase C alpha (PKCα) proteins were determined by Western blot, respectively. GFAP expression was also observed with immunocytochemistry under a fluorescence microscope. Results : LT extract induced cell proliferation in H2O2-stimulated C6 cells. LT extract significantly inhibited the expression of GFAP, NF-κB and COX-2 and increased the expression of HO-1 and the phosphorylation of STAT3 in H2O2-stimulated C6 cells. LT extract also significantly increased the phosphorylation of Akt and decreased the expression of PKCα in a dose-dependent manner in H2O2-stimulated C6 cells. Conclusions : LT extract can regulate H2O2-induced activation of astrocytes through inhibiting the expression of NF-κB, COX-2 and regulating Akt / HO-1, STAT3 or PKCα signaling pathway.
Keywords
Liriopis tuber; Liriope platyphylla; C6 astrocytes; oxidative damage; STAT3; Akt; HO-1; $PKC{\alpha}$;
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Times Cited By KSCI : 4  (Citation Analysis)
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