• Journal of Ocean Engineering and Technology
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• v.21 no.5
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• pp.55-60
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• 2007

Bellows are mechanical components which prevent the damage of system by absorption of the vibration and the displacement of axle and radial direction. Thermal piping system is expanded by the fluid of the high temperature from the heat engine inside. At this time, bellows prevent the damage of the piping due to the thermal expansion. Recently, design of bellows is required to fit some other operational environments which are not suggested in the E.J.M.A code book. And it is difficult to produce and to maintain bellows of high temperature and high pressure bemuse of its complicated shape and this causes the manufacturing cost to rise. The objective of this study is to determine optimum shape of bellows which can endure in the high temperature and high pressure. The maximum stress has an effect on the fatigue life of bellows, therefore it needs to be minimized. This study attempts to find a shape which minimizes the stress occurring in the bellows by the design of experiment. The model used in this study is not presented in the E.J.M.A code book, therefore, from the result of design of experiment we find the factors which give effects on the characteristic value and we presents the recession model using the RSM, which can predict the characteristic values depending on the change of factor values.

• Herbal Formula Science
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• v.25 no.1
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• pp.51-68
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• 2017

Objectives : Oxidative stress due to excessive accumulation of reactive oxygen species (ROS) is one of the risk factors for the development of several chronic diseases, including neurodegenerative diseases. Methods : In the present study, we investigated the protective effects of cheongnoemyeongsin-hwan (CNMSH) against oxidative stress‑induced cellular damage and elucidated the underlying mechanisms in neuronal-derived SH-SY5Y cells. Results : Our results revealed that treatment with CNMSH prior to hydrogen peroxide (H2O2) exposure significantly increased the SH-SY5Y cell viability, indicating that the exposure of the SH-SY5Y cells to CNMSH conferred a protective effect against oxidative stress. CNMSH also effectively attenuated H2O2‑induced comet tail formation, and decreased the phosphorylation levels of the histone ${\gamma}H2AX$, as well as the number of apoptotic bodies and Annexin V‑positive cells. In addition, CNMSH exhibited scavenging activity against intracellular ROS generation and restored the mitochondria membrane potential (MMP) loss that were induced by H2O2, suggesting that CNMSH prevents H2O2‑induced DNA damage and cell apoptosis. Moreover, H2O2 enhanced the cleavage of caspase-3 and degradation of poly (ADP-ribose)-polymerase, a typical substrate protein of activated caspase-3, as well as DNA fragmentation; however, these events were almost totally reversed by pretreatment with CNMSH. Furthermore, CNMSH increased the levels of heme oxygenase-1 (HO-1), which is a potent antioxidant enzyme, associated with the induction of nuclear factor-erythroid 2-related factor 2 (Nrf2). According to our data, CNMSH is able to protect SH-SY5Y cells from H2O2-induced apoptosis throughout blocking cellular damage related to oxidative stress through a mechanism that would affect ROS elimination and activating Nrf2/HO-1 signaling pathway. Conclusions : Therefore, we believed that CNMSH may potentially serve as an agent for the treatment and prevention of neurodegenerative diseases caused by oxidative stress.

• International Journal of Stem Cells
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• v.15 no.3
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• pp.311-323
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• 2022

Background and Objectives: Human mesenchymal stem cells (MSCs) are emerging as a treatment for atopic dermatitis (AD), a chronic inflammatory skin disorder that affects a large number of people across the world. Treatment of AD using human umbilical cord blood-derived MSCs (hUCB-MSCs) has recently been studied. However, the mechanism underlying their effect needs to be studied continuously. Thus, the objective of this study was to investigate the immunomodulatory effect of epidermal growth factor (EGF) secreted by hUCB-MSCs on AD. Methods and Results: To explore the mechanism involved in the therapeutic effect of MSCs for AD, a secretome array was performed using culture medium of hUCB-MSCs. Among the list of genes common for epithelium development and skin diseases, we focused on the function of EGF. To elucidate the effect of EGF secreted by hUCB-MSCs, EGF was downregulated in hUCB-MSCs using EGF-targeting small interfering RNA. These cells were then co-cultured with keratinocytes, Th2 cells, and mast cells. Depletion of EGF disrupted immunomodulatory effects of hUCB-MSCs on these AD-related inflammatory cells. In a Dermatophagoides farinae-induced AD mouse model, subcutaneous injection of hUCB-MSCs ameliorated gross scoring, histopathologic damage, and mast cell infiltration. It also significantly reduced levels of inflammatory cytokines including interleukin (IL)-4, tumor necrosis factor (TNF)-α, thymus and activation-regulated chemokine (TARC), and IL-22, as well as IgE levels. These therapeutic effects were significantly attenuated at all evaluation points in mice injected with EGF-depleted hUCB-MSCs. Conclusions: EGF secreted by hUCB-MSCs can improve AD by regulating inflammatory responses of keratinocytes, Th2 cells, and mast cells.

• Journal of Life Science
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• v.13 no.6
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• pp.950-957
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• 2003

The purpose of this study is to classify the contrast group and experiment group of a male white rat, and to identify a result of NFG's change that press the sciatic nerves injured peripheral ones for curing 1day, 3days, 5days and 7days. In contrast group, the nerve cells of immune-reaction were increased on 1 day and they were decreased for 3 days, 5 days, 7 days as a normal one. In experiment group, they were increased on 1 day but, decreased for 3 days, 5 days, 7 days more than contrast group. In conclusion, it is certain that the Needle TENS can make a NFG decrease and effect on the therapy.

• The Plant Pathology Journal
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• v.35 no.5
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• pp.521-529
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• 2019

Tomato yellow leaf curl China virus is a species of the widespread geminiviruses. The infection of Nicotiana benthamiana by Tomato yellow leaf curl China virus (TYLCCNV) causes a reduction in photosynthetic activity, which is part of the viral symptoms. ${\beta}C1$ is a viral factor encoded by the betasatellite DNA ($DNA{\beta}$) accompanying TYLCCNV. It is a major viral pathogenicity factor of TYLCCNV. To elucidate the effect of ${\beta}C1$ on plants' photosynthesis, we measured the relative chlorophyll (Chl) content and Chl fluorescence in TY-LCCNV-infected and ${\beta}C1$ transgenic N. benthamiana plants. The results showed that Chl content is reduced in TYLCCNV A-infected, TYLCCNV A plus $DNA{\beta}$ (TYLCCNV A + ${\beta}$)-infected and ${\beta}C1$ transgenic plants. Further, changes in Chl fluorescence parameters, such as electron transport rate, $F_v/F_m$, NPQ, and qP, revealed that photosynthetic efficiency is compromised in the aforementioned N. benthamiana plants. The presense of ${\beta}C1$ aggravated the decrease of Chl content and photosynthetic efficiency during viral infection. Additionally, the real-time quantitative PCR analysis of oxygen evolving complex genes in photosystem II, such as PsbO, PsbP, PsbQ, and PsbR, showed a significant reduction of the relative expression of these genes at the late stage of TYLCCNV A + ${\beta}$ infection and at the vegetative stage of ${\beta}C1$ transgenic N. benthamiana plants. In summary, this study revealed the pathogenicity of TYLCCNV in photosynthesis and disclosed the effect of ${\beta}C1$ in exacerbating the damage in photosynthesis efficiency by TYLCCNV infection.

• Journal of the Architectural Institute of Korea Structure & Construction
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• v.35 no.7
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• pp.165-170
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• 2019

The structural damage caused by earthquake to the upper structure of seismic base-isolated system can be suppressed effectively because it is designed to concentrate the input energy on the seismic isolation floor. Further, the response acceleration of seismic base-isolated system can be greatly reduced compared to the seismic structure because of the long period, which means that the design shear force of the seismic base-isolated system can be reduced appropriately. However, when the design shear force is determined to be reduced, the design stiffness will decrease, and the response acceleration will increase oppositely. Therefore, for finding the extent to which the design shear force of the upper structure can be reduced, this paper considered the seismic base-isolated structure as the analytical model and proposed the design shear force reduction factor of the base-isolated structure through the dynamic response analysis, while considering the decrement effect of response acceleration. The research result shows that the response acceleration of the isolated the upper structure can be reduced by 50%~70% of the seismic structure under the same design conditions, and the design shear force can be reduced by up to 40%. By increasing the design stiffness over to 1.8 times of the original design value, the design shear force can be reduced to the same extent as the response acceleration can be reduced compared to the seismic structure.

• Journal of the Korean Society of Safety
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• v.19 no.2
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• pp.1-5
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• 2004

Al-brass is the raw material of mnufacturing tubes for heat exchanger of vessel where seawater is used to coolant because it has high level of heat coductivity and excellent mechanical properties and high level of corrosion resistance due to cuprous oxide($Cu_2O$) layer against seawater. However, damage of Al-brass tubes for heat exchanger of vessel is reported that local corrosion such as stress corrosion cracking occurred by synergism effect between mechanical factor and corrosion environment. In this study, to investigate on the effect of stress on the polarization characteristics of Al-brass. At the stress of 0% and 95% yield strength by constant displacement tester, in 3.5% NaCl + 0.1% $NH_4OH$ solution, the polarization tests were carried out. And thus open circuit potential, corrosion current density, anodic polarization, cyclic polarization and dezincification behavior of Al-brass are investigated.

• Structural Engineering and Mechanics
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• v.31 no.6
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• pp.697-716
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• 2009

This paper summarizes an experimental and analytical study on the seismic behavior of high strength reinforced concrete columns under cyclic loading. In total six cantilever columns with different sizes and concrete compressive strengths were tested. Three columns, small size, had a $325{\times}325$ mm cross section and the three other columns, medium size, were $520{\times}520$ mm. Concrete compressive strength was 80, 130 and 180 MPa. All specimens were designed in accordance with the Japanese design guidelines. The tests demonstrated that, for specimens made of 180 MPa concrete compressive strength, spalling of cover concrete was very brittle followed by a significant decrease in strength. Curvature was much important for the small size than for the medium size columns. Concrete compressive strength had no effect on the curvature distribution for a drift varying between -2% and +2%. However, it had an effect on the drift corresponding to the peak moment and on the equivalent viscous damping variation. Simple equations are proposed for 1) evaluating the concrete Young's modulus for high strength concrete and for 2) evaluating the moment-drift envelope curves for the medium size columns knowing that of the small size columns. Experimental moment-drift and axial strain-drift histories were well predicted using a fiber model developed by the authors.

• Nuclear Engineering and Technology
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• v.54 no.7
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• pp.2702-2713
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• 2022

A steam generator tube rupture accompanying a core damage may cause the fission product to be released to environment bypassing the containment. In such an accident, the steam generator is the major path of the radioactive aerosol release. AEOLUS facility, the scaled-down model of Korean type steam generator, was built to examine the aerosol removal in the steam generator during the steam generator tube rupture accident. Integral and separate effect tests were performed with the facility for the dry and flooded conditions, and the decontamination factors were presented for different tube configurations and submergences. The dry test results were compared with the existing test results and with the analyses to investigate the aerosol retention physics by the tube bundle, with respect to the particle size and the bundle geometry. In the flooded tests, the effect of submergence were shown and the retention in the jet injection region were presented with respect to the Stokes number. The test results are planned to be used to constitute the aerosol retention model, specifically applicable for the analysis of the steam generator tube rupture accident in Korean nuclear power plants to evaluate realistic fission product behavior.

• The Korea Journal of Herbology
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• v.31 no.6
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• pp.37-44
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• 2016

Objective : This study aimed to evaluate the protective effect of Artemisiae Capillaris Herba (AC) in reflux esophagitis (RE) rats. Methods : The AC was measured antioxidant activity through in vitro experiments, such as total polyphenol and flavonoid contents, 1, 1-diphenyl-2-picrylhydrazyl (DPPH) and 2, 2'-azinobis-3-ethyl-benzothiazoline-6-sulfonic acid (ABTS) radical scavenging activity. Base on the results, we had conducted in vivo experiments. Rats were divided normal, control, AC treatment 50 mg/kg BW (AC50), and AC treatment 100 mg/kg BW (AC100) groups. AC were orally administered 2 h before the induction of RE. RE was induced by tie the pylorus and the transitional junction between the forestomach and the corpus in Sprague-Dawley rats. The rats were sacrificed 5 h after the surgery. We analyzed the expression of inflammatory related markers by western blot and observed the production of reactive oxygen species (ROS) and hematoxylin-eosin staining, Results : The $IC_{50}$ of AC for DPPH and ABTS were showed 12.60 and $33.32{\mu}g/m{\ell}$ respectively. In the RE rat, AC decreased inflammatory related markers, such as phosphorylated inhibitor of ${\kappa}B{\alpha}$, nuclear factor-kappa B, cyclooxygenase-2, inducible nitric oxide synthase, and tumor necrosis factor alpha. Also, AC reduced the increased reactive oxygen species in serum. The anti-inflammatory effect of AC appeared to be partially mediated through the inhibition of ROS. Also, AC markedly ameliorated esophageal mucosa damage via the inhibition of protein expression related to inflammation. Conclusions : Therefore, these results suggest that AC would be used as a therapeutic material in protection and/or treatment for reflux esophagitis.