The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Improving effect of Artemisiae Capillaris Herba extract in reflux esophagitis rats

인진호 추출물의 급성역류성 식도염 유발 흰쥐에 대한 개선 효과

  • Lee, Joo Young (Department of Korean Medicine, Daegu Haany University) ;
  • Seo, Bu Il (Department of Korean Medicine, Daegu Haany University) ;
  • Roh, Seong-Soo (Department of Korean Medicine, Daegu Haany University)
  • 이주영 (대구한의대학교 한의과대학 본초학교실) ;
  • 서부일 (대구한의대학교 한의과대학 본초학교실) ;
  • 노성수 (대구한의대학교 한의과대학 본초학교실)
  • Received : 2016.10.10
  • Accepted : 2016.11.16
  • Published : 2016.11.30
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Abstract

Objective : This study aimed to evaluate the protective effect of Artemisiae Capillaris Herba (AC) in reflux esophagitis (RE) rats. Methods : The AC was measured antioxidant activity through in vitro experiments, such as total polyphenol and flavonoid contents, 1, 1-diphenyl-2-picrylhydrazyl (DPPH) and 2, 2'-azinobis-3-ethyl-benzothiazoline-6-sulfonic acid (ABTS) radical scavenging activity. Base on the results, we had conducted in vivo experiments. Rats were divided normal, control, AC treatment 50 mg/kg BW (AC50), and AC treatment 100 mg/kg BW (AC100) groups. AC were orally administered 2 h before the induction of RE. RE was induced by tie the pylorus and the transitional junction between the forestomach and the corpus in Sprague-Dawley rats. The rats were sacrificed 5 h after the surgery. We analyzed the expression of inflammatory related markers by western blot and observed the production of reactive oxygen species (ROS) and hematoxylin-eosin staining, Results : The $IC_{50}$ of AC for DPPH and ABTS were showed 12.60 and $33.32{\mu}g/m{\ell}$ respectively. In the RE rat, AC decreased inflammatory related markers, such as phosphorylated inhibitor of ${\kappa}B{\alpha}$, nuclear factor-kappa B, cyclooxygenase-2, inducible nitric oxide synthase, and tumor necrosis factor alpha. Also, AC reduced the increased reactive oxygen species in serum. The anti-inflammatory effect of AC appeared to be partially mediated through the inhibition of ROS. Also, AC markedly ameliorated esophageal mucosa damage via the inhibition of protein expression related to inflammation. Conclusions : Therefore, these results suggest that AC would be used as a therapeutic material in protection and/or treatment for reflux esophagitis.

Keywords

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