• Asian-Australasian Journal of Animal Sciences
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• v.31 no.8
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• pp.1119-1126
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• 2018

Objective: Present investigation was aimed to study the Single Nucleotide Variants of the luteinizing hormone beta ($LH{\beta}$) gene and to analyze their association with the semen quality (fresh and post-thawed frozen semen) and luteinizing hormone (LH) concentrations in Murrah buffalo bulls. Methods: Polymerase chain reaction-single stranded conformational polymorphism (PCR-SSCP) and Sanger sequencing method is used to study genetic variability in $LH{\beta}$ gene. LH assay was carried out using enzyme-linked immunosorbent assay method. A fixed general linear model was used to analyze association of single nucleotide polymorphism (SNP) of $LH{\beta}$ gene with semen quality in 109 and LH concentrations in 80 Murrah bulls. Results: $LH{\beta}$ gene was found to be polymorphic. Total six SNPs were identified in $LH{\beta}$ gene g C356090A, g C356113T, g A356701G, g G355869A, g G356330C, and g G356606T. Single Stranded Conformational Polymorphism variants of pattern 2 of exon 1+pattern 2 of exon 2+pattern 1 of exon 3 had highly significant (p<0.01) effect on sperm concentration (million/mL), percent mass motility, acrosome integrity and membrane integrity in fresh and frozen semen whereas significant (p<0.05) effect was observed on percent live spermatozoa. SSCP variants of pattern 2 of exon 1+pattern 2 of exon 2+pattern 1 of exon 3 had highly significant (p<0.01) effect on luteinizing hormone concentrations too. Conclusion: The observed association between SSCP variants of $LH{\beta}$ gene with semen quality parameters and LH concentrations indicated the possibilities of using $LH{\beta}$ as a candidate gene for identification of markers for semen quality traits and LH concentrations in Murrah buffaloes.

• Korean Journal of Veterinary Service
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• v.17 no.3
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• pp.227-246
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• 1994

Present investigations were undertaken in order to clarify the clinical status of Korean native cattle and dairy cattle(holstein). Blood, Feces and urine samples were collected from 247 Korean native cattle(222 adult and 25 calf), 224 dairy cattle(211 adult and 13 calf) at Chonbuk area and analyzed for clinical, serum chemical, hematological and urinary findings. In addition, we were examined the infection rate of Theileriosis, internal patasite and ring worm. The mean value for each component was calculated by statistical analysis using Excel computer program. From these investigations the following results were obtained. The mean values for RBC, PCV and etc in 433 adult cow(Korean native cattle and dairy cattle) were similar with other reports. But the mean values for MCHC of all species were lower than normal. Adult Korean native cattle and adult dairy cattle which showed hematologically normal levels were only 9.01% and 9.48%, respectively. Ahnormally high values for PCV, RBC and Hb were recorded in 7.66% of adult Korean native cattle, 20% of Korean native calf, 15.38% of dairy calf. Adult Korean native cattle and adult dairy cattle which showed hematologically anemia were 4.95% and 19.43% respectively, but all in Korean native calf and dairy calf showed normal values. Adult Korean native cattle, adult dairy cattle, Korean native calf and dairy calf which showed normal serum protein level were 84.0%, 90.8%, 50% and 44.4%, respectively. In present investigations, 50% of Korean native calf and 55.6% of dairy calf were decreased serum protein values under normal range. These abnormally decreased serum protein values mean the shortage of antibody, and these have a possibility to occrus to pneumonia and diarrhea. From these results, the economical loss caused by pneumonia was calculated as 124, 038, 833 won in the KNC and 742, 703, 430 won in the dairy calf rearing in Chonbuk area. Calculated economical loss caused by enteritis was 56, 658, 690 won in Korean native cattle. 476, 775, 799 won in dairy calf and the total loss amount to 533, 434, 488 won in Chonbuk area. Abnormally high values($21.7{\pm}4.0mg/dl) for serum calcium were recorded 49.6% in dairy cattle. The mean values of serum total cholesterol were $170.8{\pm}99.8mg/dl in Korean native cattle, $196.0{\pm}40.6mg/dl$ in Korean native calf, $202.9{\pm}86.0mg/dl$ in adult dairy cattle and $289.4{\pm}97.5mg/dl$ in dairy calf. The infection rate of internal parasite were as follows； adult Korean native cattle：21.2%, Korean native calf：80%, adult dairy cattle：67.8%. The estimated economical loss caused by internal parasites infection were 1, 120, 855, 837 won in Korean native calf, 4, 994, 959, 405 won in adult Korean native cattle, 3, 334, 751, 066 won in adult holstein, and the total loss amount to 9, 450, 566, 308 won. The infection rate of theileriosis were 1.4% in Korean native cattle and 6.6% in dairy cattle. The presumed Economical loss by T. sergenti infection were 154, 408, 482 won in Korean native cattle and 171, 577, 237 won in dairy cattle rearing at Chonbuk area. The infection rat of ringworm were 0.5% in Korean native cattle, 0.9% in adult dairy cattle and 7.7% in dairy calf. The presumed economical loss by dermatomycophyte were 12, 061, 532 won in Korean native cattle, 16, 895, 403 won in dairy cattle, and the total estimated loss amount to 28, 955, 935 won a year in Chonbuk area. The infection rate of ringworm were 0. 5% in Korean native cattle, 0.9% in adult dairy cattle and 7.7% in dairy calf. The presumed economical loss by dermatomycophyte were 12, 061, 532 won in Korean native cattle, 16, 895, 403 won in dairy cattle, and the total estimated loss amount to 28, 955, 935 won a year in Chonbuk area.

• Asian-Australasian Journal of Animal Sciences
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• v.29 no.6
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• pp.775-781
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• 2016

A single trait linear mixed random regression test-day model was applied for the first time for analyzing the first lactation monthly test-day milk yield records in Karan Fries cattle. The test-day milk yield data was modeled using a random regression model (RRM) considering different order of Legendre polynomial for the additive genetic effect (4th order) and the permanent environmental effect (5th order). Data pertaining to 1,583 lactation records spread over a period of 30 years were recorded and analyzed in the study. The variance component, heritability and genetic correlations among test-day milk yields were estimated using RRM. RRM heritability estimates of test-day milk yield varied from 0.11 to 0.22 in different test-day records. The estimates of genetic correlations between different test-day milk yields ranged 0.01 (test-day 1 [TD-1] and TD-11) to 0.99 (TD-4 and TD-5). The magnitudes of genetic correlations between test-day milk yields decreased as the interval between test-days increased and adjacent test-day had higher correlations. Additive genetic and permanent environment variances were higher for test-day milk yields at both ends of lactation. The residual variance was observed to be lower than the permanent environment variance for all the test-day milk yields.

• Journal of Dairy Science and Biotechnology
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• v.35 no.3
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• pp.184-195
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• 2017

This study was carried out to investigate the micro-agribusiness realities of dairy farms by surveying both farmers' opinions and consumers' recognition of dairy farm products for in a value-chain analysis. In most cases, dairy farms produced fermented milk for sales and to prepare cheese both for sales and for providing the necessary material for farm visitors. Value-chain analysis did not seem to be a viable strategy for dairy farmers, except when assessing the quality of farmstead milk products in comparisons of dairy farm milks and commercial milk products. Consumers had a wide range of experience with milk products and had no willingness to buy products from dairy farms because of the high cost and the lack of product diversity. Our findings established three strategies for improving the economic feasibility of dairy farms. First, the price should be reduced by adopting a production balance to establish a farm-gate price, i.e., not as quota milk but as surplus milk. Second, consumers should be educated on the prescription of cheese at home. Third, a value-chain based on cooperative partnerships should be established among dairy farms. In addition, to improve the consumers' satisfaction on the diversity and quality of farm dairy products, developments of new value-added milk products made from the milk of cows allowed to graze on mountain pastures are needed.

• Food Science of Animal Resources
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• v.36 no.4
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• pp.499-507
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• 2016

In this study, traditional culture method and 16S rRNA gene analysis were applied to reveal the composition and diversity of lactic acid bacteria (LAB) of fermented cow milk, huruud and urum from Baotou and Bayannur of midwestern Inner Mongolia. Also, the quantitative results of dominant LAB species in three different types of dairy products from Baotou and Bayannur were gained by quantitative polymerase chain reaction (q-PCR) technology. Two hundred and two LAB strains isolated from sixty-six samples were identified and classified into four genera, namely Enterococcus, Lactococcus, Lactobacillus, Leuconostoc, and twenty-one species and subspecies. From these isolates, Lactococcus lactis subsp. lactis (32.18%), Lactobacillus plantarum (12.38%) and Leuconosto mesenteroides (11.39%) were considered as the dominated LAB species under the condition of cultivating in MRS and M17 medium. And the q-PCR results revealed that the number of dominant species varied from samples to samples and from region to region. This study clearly shows the composition and diversity of LAB existing in fermented cow milk, huruud and urum, which could be considered as valuable resources for LAB isolation and further probiotic selection.

• Journal of Dairy Science and Biotechnology
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• v.27 no.1
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• pp.29-35
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• 2009

To focus attention on milk and dairy products, we celebrate World Milk Day on the first day of June. Milk is a primary source of essential nutrients for newborn mammals, including humans. Milk and its by-products have been integral to human life since domestication of the dairy cow. Even though a large portion of the population is lactase impersistent, consumption of dairy products, including liquid milk, has been increasing over the last decade in Korea. Per capita annual consumption of dairy products in Korea increased to 61.3 kg in 2008, up from 49.4 kg in 1998. However, consumption of liquid milk has only increased marginally from 30.3 kg in 1998 to 35 kg in 2008. In 2007, the price of farm milk in Korea was the highest in the world. High dairy prices are forcing major manufacturers to find alternative, less expensive sources of milk, which has led to an increase in imported dairy products. Recently, the International Dairy Federation (IDF) published a special report containing the figures for world milk production, milk processing, and consumption. Understanding the current situation with regard to world diary production and the outlook for the industry could be useful during the present world economic crisis. The authors of this brief review summarize the status of dairy production in countries throughout the world, including Korea, along with some of the data published in the IDF annual report. The authors greatly appreciate the support of IDF Korea and the IDF for generously providing the data used in this paper.

• Asian-Australasian Journal of Animal Sciences
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• v.32 no.8_spc
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• pp.1266-1274
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• 2019

This paper presents a review of dairy goat production in sub-Saharan Africa (SSA) from 2010- 2017, its current state, constraints and prospects for research and development. Since the introduction of dairy goats in SSA in pre-colonial times, their populations have continued to increase due to declining land size as a result of land fragmentation and increasing demand for goat milk. The current goat population in SSA is 372,716,040 head of which only 15.98% used for milk production. Populations in the Eastern and Western regions of SSA have shown an increasing trend from 2010 to 2017. The Southern Africa goat population is on the decline at an annual rate of about 1.77% whereas Central Africa has had a constant goat population within the same period. Eastern Africa reported the highest increase in the population of goats used for milk production. Milk production was highest in Eastern Africa and lowest in Southern Africa. However, dairy goat productivity remained constant in the Eastern region throughout the review period. Dairy goats are mainly raised under smallholder mixed crop-livestock systems. To enhance the development of the dairy goat, concerted efforts should be made to alleviate the constraints that stifle its growth. These constraints can be categorized into nutrition and feeding, breeding and reproduction, diseases, parasites, climate change, and underdeveloped dairy goat products market. Effective management of dairy goats requires a holistic approach and there is the need to expand the markets by further sensitization on the nutritional and medicinal advantages of dairy goat products. In order to achieve rapid development in the dairy goat sub sector, research and development initiatives should be directed towards alleviating the hurdles in nutrition and feeding, breeding, animal health and resilience as well as dairy goat markets.

• Journal of agriculture & life science
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• v.51 no.6
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• pp.137-144
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• 2017

A diverse of recommendation has been made for the structure and management of dairy cows, despite demanding research, the relationship between lactation number and various factors is yet to be established. The present study was aimed to investigate the covariance among lactation number, growth performance, calving interval, and milk production was considered to increase an efficiency of selection schemes and to manage more efficiently Holstein dairy cows that have been raised on small-scale family farms in Republic of Korea. For that purpose, the data were observed from 850 Holstein dairy cows, which a total of 3929 milking, since April 2016 - January 2017. We measured the body weight, height, age, calving interval, and milk production of the each dairy cow. Also, information about the date of lactation, calving interval, and milk production was recorded using an automatic milking system(AMS) with identification numbers. Milk production was calculated per udder quarter in the AMS. Our study results showed the increased average body weight(p>0.05) in 1, 2, 3, and $4^{th}$ lactating dairy cows and afterwards, we noticed the tendency on the average body weight(p<0.05) per lactation progressed. There was no significant difference noticed on height measurement of dairy cows. From the processing data of 850 Holstein dairy cows, the lactation number 1 and 7 had a greater calving interval with significantly lowered milk production, and the lactation number 2, 3, 4, 5, and 6 had significantly lowered the calving interval(p<0.05) with a greater milk production. From our study results, we evidenced that there is a significant relationship between the lactation number, growth performance, calving interval, and milk yield, and the maximum production of milk occurring in the $3^{rd}$ and $4^{th}$ lactation dairy cows. The achieved results from this study can be used by the small-scale farmers to encourage the structure and management of growth performance, calving interval, and milk yield in Holstein dairy cows in Korea.

• Journal of Dairy Science and Biotechnology
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• v.33 no.4
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• pp.253-262
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• 2015

The authentication and implications of misleading labeling in milk and dairy products is important to protect against cheating consumers from adulteration and to alert sensitive consumers to any undeclared potential allergens. This need to support milk and dairy products labeling has led to the development of specific analytical techniques for the analysis of milk and dairy products ingredients. Recently, several methods based on polymerase chain reaction (PCR), including restriction fragment length polymorphism (PCR-RFLP), multiplex PCR, species-specific PCR, and real-time PCR, have been proposed as useful means for identifying species of origin in milk and dairy products, as well as quantifying and detecting any adulteration. These methods have particular advantages owing to their high specificity and sensitivity, as well as rapid processing time. In this review, we provide an updated and extensive overview of the PCR-based methods used for milk and dairy products authentication with a particular focus on the application of PCR methods to detect adulteration.

• Journal of Dairy Science and Biotechnology
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• v.38 no.1
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• pp.45-52
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• 2020

The advent of the Internet and social media has given rise to a number of anti-milk articles that contain a lot of misinformation. Should milk and dairy products really not be consumed past a certain age? To answer this question, the history of milk consumption must be examined to understand the long-term benefits of milk. Historically, dairy products were among the foods served to the angels in the Old Testament of the Bible. Furthermore, dairy products were consumed even before the Bronze Age, and are defined by many food and animal scientists, and nutritionists as a complete food containing a lot of nutrients. Milk and dairy products have significantly contributed to the Korean and Japanese people's current body structures; this is evident in the fact that they are taller than their ancestors, primarily due to the increased consumption of milk and dairy products following industrialization. The average height of Koreans rose drastically in the 1960s, after milk consumption increased as a result of the dairy promotion policy. This article seeks to help understand milk by looking into the content published by a life insurance company in 1927.