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http://dx.doi.org/10.5851/kosfa.2016.36.4.499

Isolation and Identification of Lactic Acid Bacteria from Traditional Dairy Products in Baotou and Bayannur of Midwestern Inner Mongolia and q-PCR Analysis of Predominant Species  

Wang, Dan (Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University)
Liu, Wenjun (Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University)
Ren, Yan (Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University)
De, Liangliang (Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University)
Zhang, Donglei (Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University)
Yang, Yanrong (Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University)
Bao, Qiuhua (Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University)
Zhang, Heping (Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University)
Menghe, Bilige (Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University)
Publication Information
Food Science of Animal Resources / v.36, no.4, 2016 , pp. 499-507 More about this Journal
Abstract
In this study, traditional culture method and 16S rRNA gene analysis were applied to reveal the composition and diversity of lactic acid bacteria (LAB) of fermented cow milk, huruud and urum from Baotou and Bayannur of midwestern Inner Mongolia. Also, the quantitative results of dominant LAB species in three different types of dairy products from Baotou and Bayannur were gained by quantitative polymerase chain reaction (q-PCR) technology. Two hundred and two LAB strains isolated from sixty-six samples were identified and classified into four genera, namely Enterococcus, Lactococcus, Lactobacillus, Leuconostoc, and twenty-one species and subspecies. From these isolates, Lactococcus lactis subsp. lactis (32.18%), Lactobacillus plantarum (12.38%) and Leuconosto mesenteroides (11.39%) were considered as the dominated LAB species under the condition of cultivating in MRS and M17 medium. And the q-PCR results revealed that the number of dominant species varied from samples to samples and from region to region. This study clearly shows the composition and diversity of LAB existing in fermented cow milk, huruud and urum, which could be considered as valuable resources for LAB isolation and further probiotic selection.
Keywords
lactic acid bacteria; traditional dairy products; 16S rRNA gene; q-PCR;
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1 Bao, Q., Yu, J., Liu, W., Qing, M., Wang, W., Chen, X., Wang, F., Li, M., Wang, H., and Lv, Q. (2012b) Predominant lactic acid bacteria in traditional fermented yak milk products in the Sichuan province of China. Dairy Sci. Technol. 92, 309-319.   DOI
2 Azadnia, P. and Khan Nazer, A. (2009) Identification of lactic acid bacteria isolated from traditional drinking yoghurt in tribes of Fars province. Iranian J. Vet. Res. 10, 235-240.
3 Bao, Q., Liu, W., Yu, J., Wang, W., Qing, M., Chen, X., Wang, F., Zhang, J., Zhang, W., and Qiao, J. (2012a) Isolation and identification of cultivable lactic acid bacteria in traditional yak milk products of Gansu Province in China. J. Gen. Appl. Microbiol. 58, 95-105.   DOI
4 Bulut, C., Gunes, H., Okuklu, B., Harsa, S., Kilic, S., Sevgi Coban, H., and Fazil Yenidunya, A. (2005) Homofermentative lactic acid bacteria of a traditional cheese, Comlek peyniri from Cappadocia region. J. Dairy Res. 72, 19-24.   DOI
5 Chen, X., Du, X., Wang, W., Zhang, J., Sun, Z., Liu, W., Li, L., Sun, T., and Zhang, H. (2010) Isolation and identification of cultivable lactic acid bacteria in traditional fermented milk of Tibet in China. Int. J. Dairy Technol. 63, 437-444.   DOI
6 Chen, Y., Liu, W., Xue, J., Yang, J., Chen, X., Shao, Y., Kwok, L. Y., Bilige, M., Mang, L., and Zhang, H. (2014) Angiotensin-converting enzyme inhibitory activity of Lactobacillus helveticus strains from traditional fermented dairy foods and antihypertensive effect of fermented milk of strain H9. J. Dairy Sci. 97, 6680-6692.   DOI
7 Liu, W., Bao, Q., Qing, M., Chen, X., Sun, T., Li, M., Zhang, J., Yu, J., Bilige, M., and Sun, T. (2012) Isolation and identification of lactic acid bacteria from Tarag in eastern inner Mongolia of China by 16S rRNA sequences and DGGE analysis. Microbiol. Res. 167, 110-115.   DOI
8 Dewan, S. and Tamang, J. P. (2007) Dominant lactic acid bacteria and their technological properties isolated from the Himalayan ethnic fermented milk products. Antonie van Leeuwenhoek 92, 343-352.   DOI
9 Gurses, M. and Erdogan, A. (2006) Identification of lactic acid bacteria isolated from Tulum cheese during ripening period. Int. J. Food Prop. 9, 551-557.   DOI
10 Lick, S., Keller, M., Bockelmann, W., and Heller, K. (1996) Optimized DNA extraction method for starter cultures from yoghurt. Milchwissenschaft 51, 183-186.
11 Liu, W., Sun, Z., Zhang, J., Gao, W., Wang, W., Wu, L., Sun, T., Chen, W., Liu, X., and Zhang, H. (2009) Analysis of microbial composition in acid whey for dairy fan making in Yunnan by conventional method and 16S rRNA sequencing. Curr. Microbiol. 59, 199-205.   DOI
12 Losio, M. N., Bozzo, G., Galuppini, E., Martella, V., Bertasi, B., Pavoni, E., and Finazzi, G. (2014) Silter cheese, a traditional Italian dairy product: A source of feasible probiotic strains. Int. J. Food Prop. 18, 492-498.
13 Marco, M. and Kleerebezem, M. (2008) Assessment of realtime RT-PCR for quantification of Lactobacillus plantarum gene expression during stationary phase and nutrient starvation. J. Appl. Microbiol. 104, 587-594.
14 Mathara, J. M., Schillinger, U., Kutima, P. M., Mbugua, S. K., and Holzapfel, W. H. (2004) Isolation, identification and characterisation of the dominant microorganisms of kule naoto: The Maasai traditional fermented milk in Kenya. Int. J. Food Microbiol. 94, 269-278.   DOI
15 Rhee, S.J., Lee, J.-E., and Lee, C.-H. (2011) Importance of lactic acid bacteria in Asian fermented foods. Microb. Cell Fact. 10, S5.   DOI
16 Olsen, K., Brockmann, E., and Molin, S. (2007) Quantification of Leuconostoc populations in mixed dairy starter cultures using fluorescence in situ hybridization. J. Appl. Microbiol. 103, 855-863.   DOI
17 Sun, T., Zhao, S., Wang, H., Cai, C., Chen, Y., and Zhang, H. (2009) ACE-inhibitory activity and gamma-aminobutyric acid content of fermented skim milk by Lactobacillus helveticus isolated from Xinjiang koumiss in China. Eur. Food Res. Technol. 228, 607-612.   DOI
18 Ouadghiri, M., Vancanneyt, M., Vandamme, P., Naser, S., Gevers, D., Lefebvre, K., Swings, J., and Amar, M. (2009) Identification of lactic acid bacteria in Moroccan raw milk and traditionally fermented skimmed milk 'lben'. J. Appl. Microbiol. 106, 486-495.   DOI
19 Passerini, D., Beltramo, C., Coddeville, M., Quentin, Y., Ritzenthaler, P., Daveran-Mingot, M.-L., and Le Bourgeois, P. (2010) Genes but not genomes reveal bacterial domestication of Lactococcus lactis. PLoS One 5, e15306.   DOI
20 Sakai, M., Ohta, H., Niidome, T., and Morimura, S. (2014) Changes in microbial community composition during production of Takanazuke. Food Sci. Technol. Res. 20, 693-698.   DOI
21 Sun, Z., Liu, W., Gao, W., Yang, M., Zhang, J., Wu, L., Wang, J., Menghe, B., Sun, T., and Zhang, H. (2010) Identification and characterization of the dominant lactic acid bacteria from kurut: The naturally fermented yak milk in Qinghai, China. J. Gen. Appl. Microbiol. 56, 1-10.   DOI
22 Takeda, S., Fujimoto, R., Takenoyama, S., Takeshita, M., Kikuchi, Y., Tsend-Ayush, C., Dashnyam, B., Muguruma, M., and Kawahara, S. (2013) Application of probiotics from Mongolian dairy products to fermented dairy products and its effects on human defecation. Food Sci. Technol. Res. 19, 245-253.   DOI
23 Wu, R., Wang, W., Yu, D., Zhang, W., Li, Y., Sun, Z., Wu, J., Meng, H., and Zhang, H. (2009) Proteomics analysis of Lactobacillus casei Zhang, a new probiotic bacterium isolated from traditional home-made koumiss in Inner Mongolia of China. Mol. Cell. Proteomics 8, 2321-2338.   DOI
24 Xu, H., Liu, W., Gesudu, Q., Sun, Z., Zhang, J., Guo, Z., Zheng, Y., Hou, Q., Yu, J., and Qing, Y. (2014) Assessment of the bacterial and fungal diversity in home-made yoghurts of Xinjiang, China by pyrosequencing. J. Sci. Food Agric. 95, 2007-2015.
25 Torres-Llanez, M., Vallejo-Cordoba, B., Díaz-Cinco, M., Mazorra-Manzano, M., and González-Córdova, A. (2006) Characterization of the natural microflora of artisanal Mexican Fresco cheese. Food Control 17, 683-690.   DOI
26 Wang, Z., Bao, Y., Zhang, Y., Zhang, J., Yao, G., Wang, S., and Zhang, H. (2013) Effect of soymilk fermented with Lactobacillus plantarum P-8 on lipid metabolism and fecal microbiota in experimental hyperlipidemic rats. Food Biophys. 8, 43-49.   DOI
27 Watanabe, K., Fujimoto, J., Sasamoto, M., Dugersuren, J., Tumursuh, T., and Demberel, S. (2008) Diversity of lactic acid bacteria and yeasts in Airag and Tarag, traditional fermented milk products of Mongolia. World J. Microbiol. Biotechnol. 24, 1313-1325.   DOI
28 Yu, J., Wang, H., Zha, M., Qing, Y., Bai, N., Ren, Y., Xi, X., Liu, W., Menghe, B., and Zhang, H. (2015) Molecular identification and quantification of lactic acid bacteria in traditional fermented dairy foods of Russia. J. Dairy Sci. (in press).
29 Yu, J., Wang, W., Menghe, B., Jiri, M., Wang, H., Liu, W., Bao, Q., Lu, Q., Zhang, J., and Wang, F. (2011) Diversity of lactic acid bacteria associated with traditional fermented dairy products in Mongolia. J. Dairy Sci. 94, 3229-3241.   DOI
30 Zamfir, M., Vancanneyt, M., Makras, L., Vaningelgem, F., Lefebvre, K., Pot, B., Swings, J., and De Vuyst, L. (2006) Biodiversity of lactic acid bacteria in Romanian dairy products. Syst. Appl. Microbiol. 29, 487-495.   DOI