• Title/Summary/Keyword: DV

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Fused Polypeptide with DEF Induces Apoptosis of Lung Adenocarcinoma Cells

  • Liang, Ai-Ling;Zhang, Ting-Ting;Zhou, Ning;Huang, Di-Nan;Liu, Xin-Guang;Liu, Yong-Jun;Tu, Zhi-Guang
    • Asian Pacific Journal of Cancer Prevention
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    • v.14 no.12
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    • pp.7339-7344
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    • 2013
  • To analyze the effects of a new unknown peptide DEF on the growth of tumor cells, a fused polypeptide TAT-DV1-DEF was designed and synthesized. The lung adenocarcinoma cell line GLC-82 treated with TAT-DV1-DEF was analyzed with a cell counting kit 8, and the location of polypeptides in cells was observed under laser confocal microscopy. The efficiency of polypeptide transfection and changes in nuclear morphology were analyzed by flow cytometry and fluorescence microscopy, respectively. Finally, the mechanism of tumor cell growth inhibition was evaluated by Western blotting. We found that TAT-DV1-DEF could significantly inhibit the growth of the lung adenocarcinoma cell line GLC-82, but not the normal human embryonic kidney cell line HEK-293. Polypeptides were found to be mostly localized in the cytoplasm and some mitochondria. The efficiency of polypeptide transfection in the two cell types was approximately 99%. Apoptotic nuclei were observed under fluorescence microscopy upon treatment with polypeptides and DAPI staining. Western blot analyses indicated that the polypeptide inhibition of tumor cell growth was apoptosis dependent. In the present study, we demonstrated that fused polypeptides could induce apoptosis of the lung adenocarcinoma cell line GLC-82, indicating that the new unknown peptide DEF has antitumor effects.

Effects of Jet-Milled Defatted Soy Flour on the Physicochemical and Sensorial Properties of Hamburger Patties

  • Cho, Hyun-Woo;Jung, Young-Min;Auh, Joong-Hyuck;Lee, Dong-Un
    • Food Science of Animal Resources
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    • v.37 no.6
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    • pp.840-846
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    • 2017
  • We investigated the physicochemical and sensorial properties of hamburger patties made with three different defatted soybean flour (DSF) preparations which differed in particle size. Coarse ($Dv_{50}=259.3{\pm}0.6{\mu}m$), fine ($Dv_{50}=91.5{\pm}0.5{\mu}m$), and superfine ($Dv_{50}=3.7{\pm}0.2{\mu}m$) DSF were prepared by conventional milling and sifting, followed by jet milling at 7 bars. Hamburger patties containing 5% of each DSF were prepared for a property analysis. The hamburger patties made with 5% superfine DSF showed the lowest cooking loss among the treatment groups (p<0.05). The patties with superfine DSF also retained the texture profile values of the control patties in terms of hardness, gumminess, springiness, and chewiness, while the addition of coarse and fine DSF increased the hardness and chewiness significantly (p<0.05). The sensorial results of quantitative descriptive analysis (QDA) indicate that the patties containing superfine DSF were softer and tenderer than the controls (p<0.05). Although the overall acceptability of the patties made with coarse and fine DSF was poor, the overall acceptability of the superfine DSF patty was the same as that of the control patty. These results suggest that superfine DSF is an excellent food material that can supply dietary fiber, while maintaining the physical characteristics and texture of hamburger patty.

Depth compression method for 3D video (3차원 영상을 위한 깊이 영상 압축 방법)

  • Nam, Jung-Hak;Hwang, Neung-Joo;Cho, Gwang-Shin;Sim, Dong-Gyu;Lee, Soo-Youn;Bang, Gun;Hur, Nam-Ho
    • Journal of Broadcast Engineering
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    • v.15 no.5
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    • pp.703-706
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    • 2010
  • Recently, a need to encode a depth image has been raising with the deployment of 3D video services. The 3DV/FTV group in the MPEG has standardized the compression method of depth map image. Because conventional depth map coding methods are independently encoded without referencing the color image, coding performance of conventional algorithms is poor. In this letter, we proposed a novel method which rearranged modes of depth blocks according to modes of corresponding color blocks by using a correlation between color and depth images. In experimental results, the proposed method achieves bits reduction of 2.2% compared with coding method based on JSVM.

IEEE13941/Linux based Media Streaming for Digital Home Services (디지털 홈 서비스를 위한 리눅스 환경의 IEEE1394 기반 미디어 스트리밍)

  • Lee Sung Yong;Lee Jae Gil;Choi Chang Yeol
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.29 no.10B
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    • pp.857-865
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    • 2004
  • Recently the interest in digital home services and its related technology has increased rapidly. Multimedia data transmission between digital devices that are connected to home network is essential function. This paper presents the design and implementation of multimedia streaming system based on IEEE1394 in Linux environment. The key features of proposed system are as follows. First, it can transmit and receive not only DV format data but also MPEG2-TS format data through IEEE1394 bus. Second, it uses device drivers that handling IEEE1394 and IEC61883 protocol transparently to reduce the complexity of communication program. Third, it applied capacity adaptation technique considering various specifications of PCs to get smooth streaming for HD data. These characteristics are tested on testbed of IEEE1394 based home network. The proposed media streaming technique can be used as a home media sever in Linux environment.

Evaluation of Nutritional Content in Convenience Store Lunchboxes by Meal Type, Price, and Store Brand (편의점 도시락의 식단형태별, 가격대별, 편의점 브랜드별 영양성분 함량 평가)

  • Park, Jin-Seon;Han, Young-Hee;Hyun, Taisun
    • Korean Journal of Community Nutrition
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    • v.25 no.4
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    • pp.280-290
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    • 2020
  • Objectives: This study investigated the menu and nutritional contents of convenience store lunchboxes, and evaluated the nutritional content by meal type, price, and store brand. Methods: In September 2019, 93 convenience store lunchboxes from the top five franchise stores were purchased. Relevant information on price, food weight, food ingredients, cooking methods, and nutrition labeling were subsequently collected. Nutritional content was evaluated based on the daily value (DV) and Index of Nutritional Quality (INQ), and energy contribution of carbohydrate, protein, fat, saturated fat and sugar was compared with the recommended range. Results: Most lunchboxes included the food groups; grains/starches, meats/fish/eggs/legumes, and vegetables. However, none provided fruits, and only a few lunchboxes provided milk/milk products. Stir-frying, deep-frying, and pan-frying were the most frequent methods of cooking. The average energy content of the lunchboxes was 736.6 kcal, whereas the average contents of protein, fat and saturated fat were higher than 40% of the DV, and sodium content was 66.8% of the DV. The contents of most nutrients in traditional type lunchboxes were higher, as compared to nutrients in onedish type lunchboxes. Considering pricing of lunchboxes, protein and sodium contents were higher in the higher-priced lunchboxes as compared to lower-priced lunchboxes, but there were no differences in the INQs. The contents of energy, protein, fat, saturated fat, and cholesterol significantly differed by brand. Conclusions: Our results indicate that convenience store lunchboxes contain high levels of protein, fat, saturated fat, and sodium. The nutritional contents differed by meal type, price, and store brand, and higher price did not imply higher nutritional quality. We propose the need to educate consumers to check nutrition labels and purchase appropriate lunchboxes. Manufacturers also need to make efforts to reduce the amounts of fat, saturated fat, and sodium, and not provide protein in excess.

Application of DV-X$\alpha$ Method to ${\gamma}$-2CaO.SiO$_2$

  • Yamaguchi, Norio;Fujimori, Hirotaka;Ioku, Koji;Goto, Seishi;Nakayasu, Tetsuo
    • The Korean Journal of Ceramics
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    • v.6 no.4
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    • pp.339-342
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    • 2000
  • In the present study, we attempted to apply DV-X$\alpha$ method to expressing the reactivity of materials. The expression of reactivity was discussed by comparison between ${\gamma}$-C$_2$G having hydraulic activity and ${\gamma}$-C$_2$S not having hydraulic activity at normal conditions. It was found that the model cluster used for calculation can finely reproduce the bulk and surface states using with and without point charge, respectively. The hydration state was also represented by placing OH ̄ on the surface of the cluster. It was calculated that the bond strength of the first layer (as surface) was bigger than that of inner layers (as bulk) for ${\gamma}$-C$_2$S while that of the first layer for ${\gamma}$-C$_2$G was smaller than that of inner layers. Subsequently a model in which OH ̄ is coordinated on Ca at the surface was also calculated. The bond strength with OH ̄ was stronger than that without OH ̄, while for ${\gamma}$-C$_2$G the bond strength with OH ̄ was weaker than that without OH ̄. From these results, it is concluded that the hydraulic activity depends on whether the bond strength for hydrated state becomes weaker than that unhydrated state or not.

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The Characteristics of Internal Waves Observed by SAR and in-situ Measurement Data Near Ocheong-Do in the Yellow Sea (SAR와 현장관측에 의한 황해 어청도 주변 해역에서의 내부파 특성)

  • 김태림;최현용
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.8 no.2
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    • pp.132-137
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    • 2003
  • Observations of internal waves in the southwest coastal waters of Korea have been made using a mooring measurement and satellite SAR together. From May 28 to May 30 in 2002, thermistor chains with RCM and ADCP mooring measurements were carried out at 10 kin west of Ocheong-Do, together with a CTD field sur-vey on the surrounding waters. Also, a SAR image was acquired on May 29 at 06:53. The data from the in-situ measurement show several internal wave packets passing through the mooring point and the SAR image reveals numbers of internal wave packets distributed around the point. Temporal and spatial characteristics of internal waves in the southwest coastal waters were analyzed using the data from mooring measurement, SAR image, and the K-dv equation. The internal waves are important phenomena in terms of physical oceanography and military as well as marine biology. They should be considered as one of important features in the southwest coastal waters in summer.

Impact of GPS-RO Data Assimilation in 3DVAR System on the Typhoon Event (태풍 수치모의에서 GPS-RO 인공위성을 사용한 관측 자료동화 효과)

  • Park, Soon-Young;Yoo, Jung-Woo;Kang, Nam-Young;Lee, Soon-Hwan
    • Journal of Environmental Science International
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    • v.26 no.5
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    • pp.573-584
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    • 2017
  • In order to simulate a typhoon precisely, the satellite observation data has been assimilated using WRF (Weather Research and Forecasting model) three-Dimensional Variational (3DVAR) data assimilation system. The observation data used in 3DVAR was GPS Radio Occultation (GPS-RO) data which is loaded on Low-Earth Orbit (LEO) satellite. The refractivity of Earth is deduced by temperature, pressure, and water vapor. GPS-RO data can be obtained with this refractivity when the satellite passes the limb position with respect to its original orbit. In this paper, two typhoon cases were simulated to examine the characteristics of data assimilation. One had been occurred in the Western Pacific from 16 to 25 October, 2015, and the other had affected Korean Peninsula from 22 to 29 August, 2012. In the simulation results, the typhoon track between background (BGR) and assimilation (3DV) run were significantly different when the track appeared to be rapidly change. The surface wind speed showed large difference for the long forecasting time because the GPS-RO data contained much information in the upper level, and it took a time to impact on the surface wind. Along with the modified typhoon track, the differences in the horizontal distribution of accumulated rain rate was remarkable with the range of -600~500 mm. During 7 days, we estimated the characteristics between daily assimilated simulation (3DV) and initial time assimilation (3DV_7). Because 3DV_7 demonstrated the accurate track of typhoon and its meteorological variables, the differences in two experiments have found to be insignificant. Using observed rain rate data at 79 surface observatories, the statistical analysis has been carried on for the evaluation of quantitative improvement. Although all experiments showed underestimated rain amount because of low model resolution (27 km), the reduced Mean Bias and Root-Mean-Square Error were found to be 2.92 mm and 4.53 mm, respectively.

Gastric Cancer Cell Growth Inhibitory Effects of Cabbage Kimchi by Fermentation and Storage Conditions (김치 발효 및 저장조건에 따른 배추김치의 위암세포 성장 억제 효과)

  • Park, Ki-Bum;Kim, Su-Gon;Oh, Chan-Ho;Jeon, Jong-In;Oh, Suk-Heung
    • The Korean Journal of Food And Nutrition
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    • v.27 no.4
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    • pp.692-698
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    • 2014
  • In this study, we investigated cancer cell growth inhibitory effects of kimchi extracts obtained from cabbage kimchi. Kimchi extracts (S46h, S47h, S48h) were obtained from the samples fermented at $15^{\circ}C$ for 46 h, 47 h, and 48 h during the first 10 days, which were subsequently stored at $-1.4^{\circ}C$ in kimchi refrigerator (hereinafter DV kimchi extracts). The samples showed a higher anti-proliferative effect against AGS (human, gastric adenocarcinoma) cell lines compared to control kimchi extract (S0h) obtained from sample stored at $-1.4^{\circ}C$ without fermentation. The DV kimchi contained higher levels of ${\gamma}$-aminobutyric acid (GABA) and ornithine compared to the control kimchi extract. Among the DV kimchi extracts, the S46h sample showed a higher anti-proliferative effect against the cancer cell growth and contained higher amount of GABA than the other kimchi samples. These results suggest that the consumption of DV kimchi can be more beneficial, as it is rich in GABA and ornithine. Therefore, it could be helpful in retarding the proliferation of cancer cells compared to the control kimchi.

7가지 멀티미디어 기능을 만끽하라

  • Park, Nam-Su
    • Digital Contents
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    • no.4 s.143
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    • pp.80-83
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    • 2005
  • 지난 해‘PMP 열풍’으로 디지털 컨버전스라는 개념이 일반인의 머리 속에 깊이 자리 잡았다. 디지털 컨버전스하면 단연 휴대폰일 것이다. 휴대폰은 어떤 모바일 기기보다 기능이 많다. 현재 이러한 휴대폰의 인기에 편승해 휴대폰 형태로 디자인 된 MP3 플레이어와 디지털카메라가 속속 시장에 모습을 드러내고 있다. 웨이키안 DV-6500은 폴더 타입 휴대폰을 연상시키는 디자인에 7가지 멀티미디어 기능을 담은 최근 경향에 충실한 제품이다.

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