• Journal of Microbiology and Biotechnology
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• v.29 no.12
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• pp.1882-1893
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• 2019

β-Glucosidases and β-xylosidases are two categories of enzymes that could cleave out non-reducing, terminal β-D-glucosyl and β-D-xylosyl residues with release of D-glucose and D-xylose, respectively. In this paper, two functional β-glucosidase Dth3 and β-xylosidase Xln-DT from Dictyoglomus thermophilum were heterologously expressed in E.coli BL21 (DE3). Dth3 and Xln-DT were relatively stable at 75℃ and were tolerant or even stimulated by glucose and xylose. Dth3 was highly tolerant to glucose with a K_i value of approximately 3 M. Meanwhile, it was not affected by xylose in high concentration. The activity of Xln-DT was stimulated 2.13-fold by 1 M glucose and 1.29-fold by 0.3 M xylose, respectively. Furthermore, the βglucosidase Dth3 and β-xylosidase Xln-DT showed excellent selectivity to cleave the outer C-6 and C-3 sugar moieties of ASI, which established an effective and green method to produce the more pharmacologically active CAG, an exclusive telomerase activator. We measured temperature, pH and dosage of enzyme using a single-factor experiment in ASI biotransformation. After optimization, the optimal reaction conditions were as follows: 75℃, pH 5.5, 1 U of Dth3 and 0.2 U of Xln-DT, respectively. Under the optimized conditions, 1 g/l ASI was transformed into 0.63 g/l CAG with a corresponding molar conversion of 94.5% within 3 h. This is the first report to use the purified thermostable and sugar-tolerant enzymes from Dictyoglomus thermophilum to hydrolyze ASI synergistically, which provides a specific, environment-friendly and cost-effective way to produce CAG.

• Journal of Korean Ophthalmic Optics Society
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• v.13 no.1
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• pp.77-81
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• 2008

Purpose: This study was to investigate the refractive state of an asian population (male: 39, female: 53) from 21 to 30 years old who visited the A optical shop at jongnogu in seoul. Methods: The visual acuity test was performed by the object and subject method. Results: Among the 184 eyes, myopia is 83.16% and emmetropia is 16.84%, respectively. As for the equivalent spheric power of myopic abnormal refractive eyes, the -m0.5Dt < spheric equivalent ${\leq}$ -2.00Dt was 40.53%, the -2.00Dt < spheric equivalent ${\leq}$ -6.00Dt was 51.63% and anything over the -6.00Dt was 7.85%. The percentages of with-the-rule, against-the-rule and oblique astigmatism among people with astigmatism were 46.67%, 35.56% and 7.77%, respectively. The average of pupillary distance in male (64.5${\pm}$2.9 mm) was greater than that in female (61.9${\pm}$2.3 mm). Conclusions: Korean opticians were provided some useful information about making up a prescription by this research.

• Journal of IKEEE
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• v.18 no.4
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• pp.586-592
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• 2014

In this paper, high efficiency power management IC(PMIC) with DT-CMOS(Dynamic threshold voltage Complementary MOSFET) switching device is presented. PMIC is controlled PWM control method in order to have high power efficiency at high current level. The DT-CMOS switch with low on-resistance is designed to decrease conduction loss. The control parts in Buck converter, that is, PWM control circuit consist of a saw-tooth generator, a band-gap reference(BGR) circuit, an error amplifier, comparator circuit, compensation circuit, and control block. The saw-tooth generator is made to have 1.2MHz oscillation frequency and full range of output swing from supply voltage(3.3V) to ground. The comparator is designed with two stage OP amplifier. And the error amplifier has 70dB DC gain and $64^{\circ}$ phase margin. DC-DC converter, based on current mode PWM control circuits and low on-resistance switching device, achieved the high efficiency nearly 96% at 100mA output current. And Buck converter is designed along LDO in standby mode which fewer than 1mA for high efficiency. Also, this paper proposes two protection circuit in order to ensure the reliability.

• The Journal of Korean Institute of Information Technology
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• v.17 no.5
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• pp.47-55
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• 2019

As the damage caused by GPS jamming has been increased, researches for detecting and preventing GPS jamming is being actively studied. This paper deals with a GPS jamming detection method using multiple GPS receiving channels and three-types machine learning techniques. Proposed multiple GPS channels consist of commercial GPS receiver with no anti-jamming function, receiver with just anti-noise jamming function and receiver with anti-noise and anti-spoofing jamming function. This system enables user to identify the characteristics of the jamming signals by comparing the coordinates received at each receiver. In this paper, The five types of jamming signals with different signal characteristics were entered to the system and three kinds of machine learning methods(AB: Adaptive Boosting, SVM: Support Vector Machine, DT: Decision Tree) were applied to perform jamming detection test. The results showed that the DT technique has the best performance with a detection rate of 96.9% when the single machine learning technique was applied. And it is confirmed that DT technique is more effective for GPS jamming detection than the binary classifier techniques because it has low ambiguity and simple hardware. It was also confirmed that SVM could be used only if additional solutions to ambiguity problem are applied.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.25 no.4B
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• pp.675-682
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• 2000

In this paper, we propose a new scheduling algorithm called the Adaptive Weighted Round Robin with Delay Tolerance (AWRR/DT). The proposed scheme is based on the per-class queueing mechanism in which a number of connections of similar characteristics are multiplexed into one class-queue. Traffic classes of the proposed method are classified into a single non-real-time traffic class and other real-time traffic classes. The proposed scheme determines the weights of classes according to the input traffic and delay characteristics of each class at the beginning of every cycle. Furthermore, this scheme incorporates a cell discarding method to reduce the QoS degradation that may be incurred by congestion of networks. We have evaluated the proposed scheme through discrete-event simulation. Simulation results indicate that the proposed scheme can reduce the average delay of non-real-time class while maintaining the QoS of real-timeclasses. The proposed algorithm can be effectively applied to high-speed networks such as ATM networks.

• Journal of Ocean Engineering and Technology
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• v.5 no.2
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• pp.207-207
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• 1991

The crack length measurements by electrical potential(EP) method for 1% Cr-Mo-V and 12%Cr steel of 0.5T-CT specimen were performed at $500^{\circ}C, 600^{\circ}C 700^{\circ}C$, and an applicability of stress intensity factor($K_I$), net section stress($\sigma_{net}$), $C^*$-ingegral and $C_t$ parameter was studied to measure creep crack growth rate(da/dt) with side groove and without side groove under static and cyclic loading condition. The experimental result could be summarized as follows: 1) Crack measurement by EP method was available and coincided with the Johnson,s analytical equation. 2) da/dt by $K_I$ and $\sigma_{net}$ was not adequate because of the wide scatter band according to load and temperature, but $C^*$-integral, except for transition region, was adequate. 3) $C_t$ parameter showed the best fitted line through total creep region without relating with both temperature and load condition. 4) Under the cyclic loading condition, $C_t$ parameter was proper to extimate da/dt. And it was shown that da/dt for 1% Cr-Mo V steel under the static condition(R=1) was 1.16 times faster than the case under cyclic loading(R=0), and for 12% Cr steel, 1.43 times.

• Journal of Ocean Engineering and Technology
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• v.5 no.2
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• pp.67-75
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• 1991

The crack length measurements by electrical potential(EP) method for 1% Cr-Mo-V and 12%Cr steel of 0.5T-CT specimen were performed at $500^{\circ}C, 600^{\circ}C 700^{\circ}C$, and an applicability of stress intensity factor($K_I$), net section stress($\sigma_{net}$), $C^*$-ingegral and $C_t$ parameter was studied to measure creep crack growth rate(da/dt) with side groove and without side groove under static and cyclic loading condition. The experimental result could be summarized as follows: 1) Crack measurement by EP method was available and coincided with the Johnson, s analytical equation. 2) da/dt by $K_I$ and $\sigma_{net}$ was not adequate because of the wide scatter band according to load and temperature, but $C^*$-integral, except for transition region, was adequate. 3) $C_t$ parameter showed the best fitted line through total creep region without relating with both temperature and load condition. 4) Under the cyclic loading condition, $C_t$ parameter was proper to extimate da/dt. And it was shown that da/dt for 1% Cr-Mo V steel under the static condition(R=1) was 1.16 times faster than the case under cyclic loading(R=0), and for 12% Cr steel, 1.43 times.

• Journal of international Conference on Electrical Machines and Systems
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• v.1 no.1
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• pp.84-90
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• 2012

Various methods have been proposed to identify the flux position in an interior permanent magnet synchronous motor (IPMSM) without the use of mechanical sensors. To achieve this, a method that uses both the back electromotive force (EMF) and the saliency to identify the flux position in the IPMSM without the injection of high-frequency components at low speeds has been reported. This method was then extended in order to drive the motor with no load to a light load. We propose a combination of these methods with different proportional-integral (PI) controllers for controlling $di_{dest}$/dt and $di_{qest}$/dt. We also introduce compensation values $F_L$ and $F_H$ to reduce the position error when the estimation rule is being selected.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2004.05a
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• pp.74-76
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• 2004

It is the new promising method of obtaining the information about the state of a surface of a solid body, thin and multilayer structures. An idea of the method consists in reading and analyzing the relations U(t), dU/dt(t), dU/dt(U) on an electrical cell when anodizing an investigated object. By these relation it is possible to control the presence of impurities in the metal on the path of the anodization front, a structure and characteristics of the object being oxidized as well as of an oxide which is being formed during anodizing, the change in composition of the oxide layer, the thickness and composition of metallic and dielectric layers being a part of the layers boundaries.

• Structural Engineering and Mechanics
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• v.28 no.2
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• pp.221-238
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• 2008

Numerical solution to buckling analysis of beams and columns are obtained by the method of differential quadrature (DQ) and harmonic differential quadrature (HDQ) for various support conditions considering the variation of flexural rigidity. The solution technique is applied to find the buckling load of fully or partially embedded columns such as piles. A simple semi- inverse method of DQ or HDQ is proposed for determining the flexural rigidities at various sections of non-prismatic column ( pile) partially and fully embedded given the buckling load, buckled shape and sub-grade reaction of the soil. The obtained results are compared with the existing solutions available from other numerical methods and analytical results. In addition, this paper also uses a recently developed technique, known as the differential transformation (DT) to determine the critical buckling load of fully or partially supported heavy prismatic piles as well as fully supported non-prismatic piles. In solving the problem, governing differential equation is converted to algebraic equations using differential transformation methods (DT) which must be solved together with applied boundary conditions. The symbolic programming package, Mathematica is ideally suitable to solve such recursive equations by considering fairly large number of terms.