• The Journal of Korean Society of Virology
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• v.27 no.2
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• pp.239-255
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• 1997

Expression of the cDNA of the VP1 gene on the genome RNA B segment of infectious pancreatic necrosis virus (IPNV) DRT strain in E. coli and a recombinant baculovirus were carried out. The VP1 gene in the pMal-pol clone (Lee et al. 1995) was cleaved with XbaI and transferred into baculovirus transfer vector, pBacPAK9 and it was named pBacVP1 clone. The VP1 gene in the pBacVP1 clone was double-digested with SacI and PstI and then inserted just behind T5 phage promoter and the $6{\times}His$ region of the pQE-3D expression vector, and it was called pQEVPl. Again, the $6{\times}$His-tagged VP1 DNA fragment in the pQEVP1 was cleaved with EcoRI and transferred into the VP1 site of the pBacVP1, resulting pBacHis-VP1 recombinant. The pBacHis-VP1 DNA was cotransfected with LacZ-Hyphantria cunea nuclear polyhedrosis virus (LacZ-HcNPV) DNA digested with Bsu361 onto S. frugiperda cells to make a recombinant virus. One VP1-gene inserted recombinant virus was selected by plaque assay. The recombinant virus was named VP1-HcNPV-1. The $6{\times}$His-tagged VP1 protein produced by the pQEVP1 was purified with Ni-NTA resin chromatography and analyzed by SDS-PAGE and Western blot analysis. The molecular weight of the VP1 protein was 94 kDa. The recombinant virus, VP1-HcNPV-1 did not form polyhedral inclusion bodies and expressed VP1 protein with 95 kDa in the infected S. frugiperda cells, which was detected by Western blot. The titer of the VP1-HcNPV-1 in the first infected cells was $2.0{\times}10^5\;pfu/ml$ at 7 days postinfection.

• Journal of Biosystems Engineering
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• v.23 no.1
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• pp.49-56
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• 1998

The objectives of this study were to develop models to predict the contents of moisture and protein of single kernel of brown rice based on visible/NIR (near-infrared) spectroscopic technique. The reflectance spectra of rice were obtained in the range of the wavelength 400 to 2,500 nm with 2 nm intervals. Multiple linear regression(MLR) and partial least squares (PLS) were used to develop the models. The MLR model using the first derivative spectra(10 nm of gap) with Standard Normal Variate and Detrending (SNV and Drt.) preprocessing showed the best results to predict moisture content of the sin린e kernel brown rice. To predict the protein content of a single kernel of brown ricer the PLS model used the raw spectra with multiplicative scatter correction(MSC) preprocessing over the wavelength of 1,100~1,500 nm.

• Language and Information
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• v.8 no.2
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• pp.1-25
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• 2004

This paper examines two different approaches to resolving a theoretical problem which the bottom-up approach version of Discourse Representation Theory of Kamp et al. (2003) faces in dealing with anaphoric relations between pronouns and their potential antecedents in conditional sentences where consequent clauses precede their corresponding conditional clauses. In one of the approaches, every element is processed in the order of occurrence and conditional operators in a non-sentence-initial position cause the ongoing DR to split in two with the same index. The definition of accessibility is accordingly modified so that the right DR can be accessible from the left DR. In the other approach, a different type of discourse representation structure, K ${\Leftarrow}$ K, is introduced, which allows us to resolve the target problem without modifying accessibility proposed in Kamp et al. (2003). Compatibility of these two approaches with the bottom-up version of DRT is evaluated by examining their applicability to the analysis of quantified sentences where pronominal expressions precede generalized quantifiers.

• Language and Information
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• v.3 no.2
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• pp.67-83
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• 1999

This paper attempts to provide an account for the problems of intensional opacity of referring expressions and the presupposition inheritance in the belief contexts from the discourse perspective. I discuss Jaszczolt's discourse model based on DRT to account for the belief reports. Jaszczolt analyzes referring expressions in terms of the three readings(de re, de $dicto_1$ and de $dicto_2$) and attempts to represent the differences between them in the DRS's via different anchoring modes; external anchoring, formal anchoring and nonanchoring. I propose an extended model to account for the presupposition inheritance in the belief contexts and attempt to analyze the data in Korean based on this model. The differences in the PI and in the representations of DRS's which are induced by the different complement types, ${\ldots}ko(mitta)\;and\;{\ldots}kesul(mitta)$, are discussed.

• Proceedings of the Korean Society for Language and Information Conference
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• 2007.11a
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• pp.42-53
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• 2007

Looking back the history of formal treatment of linguistics, we cannot disregard the contribution of possible world semantics. Intensional logic of Montague semantics, DRT (Discourse Representation Theory), mental space, and situation theory are closely related to or compared with the notion of possible world. All these theories have commonly clarified the structure of belief context or uncertain knowledge, employing hypothesized worlds. In this talk, I firstly brief the pedigree of these theories. Next, I will introduce the recent development of modal logic for the representation of (i) knowledge and belief and (ii) time, in which belief modality is precisely discussed together with the accessibility among possible worlds. I will refer to BDI (belief-desire-intention) logic, CTL (computational tree logic), and sphere-based model in belief revision. Finally, I will discuss how these theories could be applied to the further development of analyses of natural language.

• Journal of Bio-Environment Control
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• v.22 no.3
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• pp.262-269
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• 2013

This study was carried out in order to examine the germination characteristics of Ligularia fischeri seeds, and it was to develop the more efficient pre-treatment and production system of the seeds. It was performed by two ways - temperature control (10, 15, 20 and $25^{\circ}C$) and shading treatment (Full sunlight, 35%, 50%, 75% and 95% of full sunlight). Seed pre-treatment before the each experiment was carried out by temperature (with low temperature and wetting treatment (LTW) for 0, 15, 30, 45 and 60days) and shading treatment (with drying at room temperature (DRT), drying at low temperature (DLT) and water soaking (WS) for 2 days). Seeds of L. fischeri were, regardless of seed pre-treatment, germinated well at $10^{\circ}C$ and the more temperature went up, the more germination rate went down. As a result of surveying shading treatment, 75% shading with DRT was the highest germination rate (68.1%) and 95% shading with WS was the lowest germination rate (48.6%). It was showed over-growth under 95% shading treatment experiment and withered in the full sunlight. As a result of surveying the whole experiment, L. fischeri seeds pre-treated with LTW for 15 days germinated well at $10^{\circ}C$ and under 50~75% shading treatment.

• Journal of the Korean Society of Radiology
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• v.6 no.5
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• pp.395-402
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• 2012

4D-Radiation Therapy is the optimal treatment to track moving organs(tumor) and give the appropriate prescription dose to tumor and low radiation dose to normal tissue surrounding tumor volume. The ABCHES is a 4DRT devices maintaining shallow breathing to patients. It allows the tumor's movement was minimize. Meanwhile, Abdominal compression device is limited the breath compressing abdomen on patients. In this paper we will quantitative analysis the movement of tumor on only ABCHES versus ABCHES with Abdomal compression device and Analysis tumor dose and normal tissue's dose by Dose Volume Histogram on two parts. The result of Comparision ABCHES and ABCHES with Abdominal compression device, SI(Superior-Inferior) direction, AP(Anterior-Posterior) direction and LR(Left-Right) direction was limited 1.0 mm, 0.2 mm, 0.2 mm(average). and also reduction rate of voluume in HPTV was $16{\pm}2%$, and LPTV was $15.8{\pm}0.8%$ under only using ABCHES and ABCHES with compression. The analysis dose volume histogram was more radiation dose in ABCHES and abdominal compression device than only using ABCHES, and less normal tissue-ipsilateral lung, whole lung, kidney-dose in ABCHES and abdominal compression device than only using ABCHES. The overall analysis was ABCHES with abdominal compression better than only using ABCHES method. In hereafter it will be studies that limitation of ABCHES and abdomonal compression device. In other words, patient's discomfort on compression intensity, method of application on patient with inaccurate respiration cycle.

• Journal of Korean Society for Geospatial Information Science
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• v.11 no.3 s.26
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• pp.23-34
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• 2003

Currently, the efficiency of GPS has been increased in the various precise survey like as the control survey and the navigation etc. Also, it is widely used in the deformation analysis of the structure, the measurement of the marine tides, the measurement of the river level and the topographic monitoring of seabed or riverbed by combined the measurement equipment for depth. In this study, we intend to increase in efficiency of the topographic monitoring of seabed or riverbed by combined with DGPS, RTK GPS and echo sounder. For this study, we defined the error correction of the echo sounder with the experiment of water tank which is considered the characteristic of estuary riverbed and then we developed the s/w for 3-dimensional monitoring of estuary riverbed and applied the s/w to field test and improved the various problems. On analyzing topography of estuary riverbed by combined GPS with echo sounder, the draught error which is yielded to change of length from the water surface by the movement of survey vessel to the end of the transducer was eliminated by geometrical rearrangement and we defined the correction formula $z=BM+SAH-DBR_{(i)}-DRT-ED$. The sounding error about the echo sounder and characteristic of estuary riverbed was found by understanding the relation of average diameter ind residual error and we defined correction formula, $Y=0.00474{\ast}ln(X)-0.0045$ by the regression analysis. and then we verified applicability of correction formula.

• Journal of the Korean Association of Geographic Information Studies
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• v.25 no.4
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• pp.107-118
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• 2022

As connected and automated driving(CAD) technology is entering its commercialization stage, service platforms providing CAD-based mobility services have increased these days. However, CAD-baded mobility services with these platforms need more consideration for the demand for mobility services when determining target areas for CAD-based mobility services because current CAB-based mobility design focus on driving performance and driving stability. For a more efficient design of CAD-based mobility services, we analyzed the applicability for the introduction of CAD-based mobility services in terms of driving difficulty of CAD and demand patterns of current non-CAD based-mobility services, e.g., taxi, demand-responsive transit(DRT), and special transportation systems(STS). In addition, for the spatial analysis of the applicability of the CAD-based mobility service, we propose the Index for Autonomous Driving Applicability (IADA) and analyze the characteristics of the spatial distribution of IADA from the network perspective. The analysis results show that the applicability of CAD-based mobility services depends more on the demand patterns than the driving difficulty of CAV. In particular, the results show that the concentration pattern of demand in a specific road link is more important than the size of demand. As a result, STS service shows higher applicability compared to other mobility services, even though the size of demand for this mobility service is relatively small.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.59 no.4
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• pp.406-412
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• 2014

The influence of rainfall during the ripening stage on pre-harvest sprouting, seed viability, and seed quality was investigated in two Korean rice cultivars, Shindongjin and Hopum. When the rainfall was artificially treated in a greenhouse, HP started to pre-harvest sprouting at three days of rainfall treatment (DRT), but Shindongjin did not show pre-harvest sprouting at 40 DAH treatment and just 0.3~0.8% at 50 DAH, which was much lower than 15.3~25.8% of Hopum in the same treatment. After harvest, the seed germination of Hopum decreased about 10~25% compared to non-treated seeds, but that of Shindongjin decreased much little rate than that of Hopum. The seed longevity tested by accelerated aging decreased with prolonged rainfall period in both cultivars, but the varietal difference was clear; Shindongjin could withstand longer accelerated aging than Hopum. Shindongjin maintained its germination (>50%) ability after 15 days of accelerated aging regardless of the rainfall treatment period and time, but Hopum dropped below 50% germination ability after only 5 days of accelerated aging. In conclusion, rainfall during the ripening stage induced not only pre-harvest sprouting, but also reduced seed quality and longevity during storage, which varied between two cultivars.