• Title/Summary/Keyword: DRP1

Search Result 49, Processing Time 0.033 seconds

Drp1 Expression and Phosphorylation in Steroidogenic Corpus Luteum during the Estrous Cycle in Rat Ovaries

  • Park, Ji-Eun;Lee, Seung Gee;Yoo, Young Hyun;Kim, Jong-Min
    • Development and Reproduction
    • /
    • v.26 no.2
    • /
    • pp.71-77
    • /
    • 2022
  • In response to luteinizing hormone (LH), a higher concentration of progesterone (P4) is produced in luteal cells of corpus luteum (CL). Mitochondria are an essential cellular organelle in steroidogenesis. The specific engagement of the concept regarding mitochondrial shaping with early stages of steroidogenesis was suggested in reproductive endocrine cells. Although the specific involvement of GTPase dynamin-related protein 1 (Drp1) with steroidogenesis has been demonstrated in luteal cells of bovine CL in vitro, its actual relationship with ovarian steroidogenesis during the estrous cycle remains unknown. In this study, while Fis1 and Opa1 protein levels did not show significant changes during the estrous cycle, Drp1, Mfn1, and Mfn2 proteins exhibited relatively lower levels at proestrus than at estrus or diestrus. 3β-HSD showed higher levels at proestrus than at estrus or diestrus. In addition, Drp1 phosphorylation (s637) was higher in proestrus than in estrus or diestrus. Immune-positive cells for Drp1, pDrp1 (s637), and 3β-HSD were all localized in the cytoplasm of luteal cells in the CL. The immune-positive cells for 3β-HSD were more frequently seen in the CL at proestrus than at estrus or diestrus. Immunoreactivity for Drp1 in luteal cells at proestrus was weaker than that at estrus or diestrus. However, pDrp1 (s637) immune-positive cells were mostly detected in luteal cells at proestrus. These results imply that steroidogenesis (P4 production) in the CL is closely related to phosphorylation of Drp1 at serine 637. Taken together, this study presents evidence that Drp1 phosphorylation at serine 637 is an important step in steroidogenesis in the CL.

A MAC Design for Collision Avoidance in Wireless USB Home Networks (WUSB 홈네트워크에서의 충돌회피를 위한 MAC설계)

  • Sim, Jae-Hwan
    • The Journal of the Korea institute of electronic communication sciences
    • /
    • v.8 no.1
    • /
    • pp.55-64
    • /
    • 2013
  • The USB-IF has specified a Wireless USB (WUSB) protocol based on UWB for high speed wireless home networks and WPANs. In this paper, firstly, performance degradation due to the Private Distributed Reservation Protocol (DRP) conflict problem caused by devices' mobility is analyzed. And a novel relay transmission protocol combined with Private DRP conflict resolution is proposed to overcome the performance degradation at Private DRP conflicts. In order to give the loser device due to Private DRP conflicts another chance to maintain resources, the proposed relay transmission protocol executed at each device helps the loser device reserve another indirect link maintaining the required resources via a relay node.

A Study on DRP System for Logistics Improvement - A Case of a Multi-sourcing Logistics System (물류개선을 위한 DRP시스템에 관한 연구 - 복수공급원이 있는 물류시스템을 중심으로-)

  • 장융남
    • Korean Management Science Review
    • /
    • v.11 no.1
    • /
    • pp.73-90
    • /
    • 1994
  • Recently the recongnition of Logistics becomes important to enterpries as a means for improving their competition, but the Korea enterpries falls for far behind in management techniques for analyzing realities and problems in the Logistics compare to the advanced countries. Especially the function of inventory control which is most undeveloped in them should be improved in Korea. Inventory is very important in Logistics, but there exists trade-off between inventory level and customer in the service level. It is difficult to coordinate both the reduction in the inventory level and the enhancement in the service level. Its effective technique is a DRP system. In general, it deals with arborescent distribution network in which a given channel member has a single source of supply. This article aims to present a DRP system, and more a DRP system which is flexible and responsive in the multisourcing, multi-echelon distribution network. Related research issues are discussed. Finally, using a DRP system on logistics and the management performance. So we can help the physical distribution improvement of the Korean enterprises.

  • PDF

Chemical Composition and Nutritional Evaluation of Variously Treated Defatted Rice Polishing for Broiler Feeding

  • Khalique, A.;Lone, K.P.;Pasha, T.N.;Khan, A.D.
    • Asian-Australasian Journal of Animal Sciences
    • /
    • v.16 no.6
    • /
    • pp.873-879
    • /
    • 2003
  • The study was conducted to improve the nutritive value of defatted rice polishing (DRP). DRP was treated with various concentrations of HCl, NaOH, $H_2O_2$ and Kemzyme-H $F^{(R)} and the effect on its chemical composition and nutritive value in broiler chicks was observed. The treatments levels of 0.4 N HCl, 0.2 N NaOH and 6% $H_2O_2$ were selected from many concentrations of HCl, NaOH and $H_2O_2$ tried earlier on DRP. The selection was made on the basis of release of nutrients from DRP. The Kemzyme-H $F^{(R)} was used at rate of 0.1% of DRP. The selected concentrations of HCl, NaOH, and $H_2O_2$ were then used for treatment of DRP that was used in biological experiments. Two hundred and forty, day-old Hubbard male broiler chicks (38-40 g) were randomly divided into 48 experimental units with five chicks each. Each chemically treated DRP was incorporated into broiler diets at 10, 20 or 30% levels replacing yellow corn from the control feed and thus sixteen experimental feeds were prepared. These feeds were randomly assigned to 48 experimental units such that there were three replicates of chicks on each diet. The results of the study suggest that DRP can be effectively used in broiler diets at 20% level. The best weight gain and feed conversion ratio were observed with diet containing 20% level of DRP treated with 6% $H_2O_2$. The diets containing 30% levels of treated DRP were uneconomical, as excess use of oil was required to compensate the energy needs of the birds.

A WD40 Repeat Protein, Arabidopsis Sec13 Homolog 1, May Play a Role in Vacuolar Trafficking by Controlling the Membrane Association of AtDRP2A

  • Lee, Myoung Hui;Lee, Sung Hoon;Kim, Heyran;Jin, Jing Bo;Kim, Dae Heon;Hwang, Inhwan
    • Molecules and Cells
    • /
    • v.22 no.2
    • /
    • pp.210-219
    • /
    • 2006
  • Dynamin-related protein 2A (AtDRP2A, formally ADL6), a member of the dynamin family, is critical for protein trafficking from the TGN to the central vacuole. However, the mechanism controlling its activity is not well understood in plant cells. We isolated Arabidopsis sec13 homolog1 (AtSeh1) that interacts with AtDRP2A by a yeast two-hybrid screening. AtSeh1 has four WD40 motifs and amino acid sequence homology to Sec13, a component of COPII vesicles. Coimmunoprecipitation and protein pull-down experiments demonstrated specific interaction between AtSeh1 and AtDRP2A. AtSeh1 bound to the pleckstrin homology domain of AtDRP2A in competition with the C-terminal domain of the latter, and this resulted in inhibition of the interaction between AtDRP2A and PtdIns3P in vitro. AtSeh1 localized to multiple locations: the nucleus, the prevacuolar compartment and the Golgi complex. Based on these results we propose that AtSeh1 plays a role in regulating cycling of AtDRP2A between membrane-bound and soluble forms.

Effects of Fly Ash and Gypsum Mixture on Reducing Phosphorus Loss from Paddy Soil (논 토양에서 석탄회와 석고의 혼합제를 활용한 인산유출 저감)

  • Lee, Yong-Bok;Lee, Seul-Bi;Oh, Ju-Hwan;Lee, Chang-Hoon;Hong, Chang-Oh;Kim, Pil-Joo
    • Korean Journal of Environmental Agriculture
    • /
    • v.27 no.1
    • /
    • pp.66-71
    • /
    • 2008
  • Phosphorus transfer from agricultural soils to surface waters is an important environmental issue. Fly ash and phospho-gypsum which are industrial by-product were investigated as a means of reducing dissolved phosphorus in arable soil. To determine the optimum mixing ratio of fly ash(FA) and phospho-gypsum(PG) for reducing dissolved reactive P(DRP) in soil, various mixture ratio of FA and PG were mixed with two soil. The DRP content and pH in soils were analysed after 3 weeks incubation under flooding condition. Although DRP content in soils was significantly decreased by FA-PG mixture compared with control, there were no significant difference among the FA and PG mixture ratio of 75:25, 50:50, and 25:75. The mixture of 75% FA and 25% PG was selected for field test. A field experiment was carried out to evaluate the reducing DRP content in paddy soil to which 0(NPK), 20(FG 20), 40(FG 40), and 60(FG 60) Mg $ha^{-1}$ of the mixture were applied. The DRP content was reduced by 31% at the application rate of 60 Mg $ha^{-1}$. In contrast to deceasing DRP, Ca-P content increased significantly with the mixture application rate. After rice harvesting, available $SiO_2$, P, and exchangeable Ca content in soil increased significantly with application rate due to high content of Si, P, and Ca in the mixture. Mixtures of fly ash and gypsum should reduce P loss from paddy soil and increase soil fertility.

Evidence for the Drp1-dependent Mitochondrial Fission in the Axon of the Rat Cerebral Cortex Neurons (흰쥐 대뇌 피질 신경세포의 축삭에서 Drp1 의존적 미토콘드리아의 분열)

  • Cho, Bong-Ki;Lee, Seung-Bok;Sun, Woong;Kim, Young-Hwa
    • Applied Microscopy
    • /
    • v.41 no.4
    • /
    • pp.249-255
    • /
    • 2011
  • Neurons utilize a large quantity of energy for their survival and function, and thereby require active mitochondrial function. Mitochondrial morphology shows dynamic changes, depending on the cellular condition, and mitochondrial dynamics are required for neuronal development and function. In this study, we found that the length of mitochondria in the distal axon is significantly shorter than that of mitochondria in dendrites or proximal axons of cerebral cortical neurons, and the reason for this difference is the local fission within the axon. We also found that suppression of Drp1, a key regulator of mitochondrial fission, resulted in significant elongation of mitochondria in axons. Collectively, these results suggest that local mitochondrial fission within the axon contributes to region-dependent mitochondrial length differences in the axons of cortical neurons.

The coordinated regulation of mitochondrial structure and function by Drp1 for mitochondrial quality surveillance

  • Cho, Hyo Min;Sun, Woong
    • BMB Reports
    • /
    • v.52 no.2
    • /
    • pp.109-110
    • /
    • 2019
  • Mitochondrial morphology is known to be continuously changing via fusion and fission, but it is unclear what the biological importance of this energy-consuming process is and how it develops. Several data have suggested that mitochondrial fission executed by Drp1 is necessary to select out a damaged spot from the interconnected mitochondrial network, but the precise mechanism for the recognition and isolation of a damaged sub-mitochondrial region during mitochondrial fission is yet unclear. Recently, Cho et al. found that the mitochondrial membrane potential (MMP) is transiently reduced by the physical interaction of Drp1 and mitochondrial Zinc transporter, Zip1, at the fission site prior to the typical mitochondrial division, and we found that this event is essential for a mitochondrial quality surveillance. In this review, Cho et al. discuss the role of a mitochondrial fission in the mitochondrial quality surveillance system.

Analysis of Characteristics of Coupled Phosphorus in the Sewage (하수 중 인의 결합 특성 분석)

  • Choi, Hee-Jeong;Lee, Seung-Mok
    • Journal of Korean Society on Water Environment
    • /
    • v.23 no.1
    • /
    • pp.97-102
    • /
    • 2007
  • The present investigation deals to achieve an accurate determination of the phosphorous present in the wastewater samples using the membrane reactor. The study may enable to quantify the dissolved (DP) and adsorbed phosphorous (AP), also the adsorbed phosphorous categorically identified as inorganic coupled phosphorous (DRP) and organic coupled phosphorous (NRP). Moreover, the study has been conducted separately in anaerobic and aerobic chamber. The results obtained showed that dissolved phosphorous only can occur in anaerobic chamber with ca. 25%. The study conducted for adsorbed phosphorous showed that the DRP has the percent composition in anaerobic and aerobic chamber respectively 33% and 40% i.e., 7% more in aerobic chamber. The similar values obtained for NRP was found to be 42% and 60% i.e., 18% more in aerobic chamber. On the other hand while comparing the results for NRP and DRP, it has to be noted that NRP has 9% and 20% more percent composition than DRP respectively in anaerobic and aerobic chamber. Further, the adsorbed phase showed the species Al-P, Fe-P in the aerobic chamber with the quotient of 7.73 mg/g TS (total solid) whereas in the anaerobic chamber it showed the species Fe-P and $Fe(OH)_3$-P with the 7.16 mg/g TS.

On Suppressing the Occurrence of Redundant Sensing-Reporting Packets in Assets Monitoring Networks (관심 대상모니터링 네트워크에서의 중복된 감지-보고 패킷들의 발생 억제에 관한 연구)

  • Tscha, Yeong-Hwan
    • Journal of the Korea Institute of Information and Communication Engineering
    • /
    • v.13 no.9
    • /
    • pp.1955-1963
    • /
    • 2009
  • In the application of wireless sensor networks to monitor valuable assets under concern, the appearance of a certain asset can be identified and reported simultaneously by several adjacent nodes, and this may dissipate the power of related nodes and network bandwidth as well. In the proposed method, a node that senses the appearance of some asset is allowed to send the sensing-reporting packet only after it has been being idle for a certain period without receiving any DRP(Don't Report Packet) from neighbors. It turns out that not only the cost of propagating DRPs but also that of reporting the events to the sink becomes minimal when depth of each DRP propagation is 2. In case of depth 1, at least, two routes are set up for the delivery of the sensing-reporting packet hence, reliable transfer to the sink is provided.