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A WD40 Repeat Protein, Arabidopsis Sec13 Homolog 1, May Play a Role in Vacuolar Trafficking by Controlling the Membrane Association of AtDRP2A  

Lee, Myoung Hui (Center for Plant Intracellular Trafficking and Division of Molecular and Life Sciences, Pohang University of Science and Technology)
Lee, Sung Hoon (Center for Plant Intracellular Trafficking and Division of Molecular and Life Sciences, Pohang University of Science and Technology)
Kim, Heyran (Center for Plant Intracellular Trafficking and Division of Molecular and Life Sciences, Pohang University of Science and Technology)
Jin, Jing Bo (Center for Plant Intracellular Trafficking and Division of Molecular and Life Sciences, Pohang University of Science and Technology)
Kim, Dae Heon (Center for Plant Intracellular Trafficking and Division of Molecular and Life Sciences, Pohang University of Science and Technology)
Hwang, Inhwan (Center for Plant Intracellular Trafficking and Division of Molecular and Life Sciences, Pohang University of Science and Technology)
Publication Information
Molecules and Cells / v.22, no.2, 2006 , pp. 210-219 More about this Journal
Abstract
Dynamin-related protein 2A (AtDRP2A, formally ADL6), a member of the dynamin family, is critical for protein trafficking from the TGN to the central vacuole. However, the mechanism controlling its activity is not well understood in plant cells. We isolated Arabidopsis sec13 homolog1 (AtSeh1) that interacts with AtDRP2A by a yeast two-hybrid screening. AtSeh1 has four WD40 motifs and amino acid sequence homology to Sec13, a component of COPII vesicles. Coimmunoprecipitation and protein pull-down experiments demonstrated specific interaction between AtSeh1 and AtDRP2A. AtSeh1 bound to the pleckstrin homology domain of AtDRP2A in competition with the C-terminal domain of the latter, and this resulted in inhibition of the interaction between AtDRP2A and PtdIns3P in vitro. AtSeh1 localized to multiple locations: the nucleus, the prevacuolar compartment and the Golgi complex. Based on these results we propose that AtSeh1 plays a role in regulating cycling of AtDRP2A between membrane-bound and soluble forms.
Keywords
Arabidopsis Sec13 Homolog; Dynamin-related Protein 2A; Phospholipid Binding; Protein-Protein Interaction;
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